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Biomedical and Environmental Sciences 2022年第12期35卷 1106-1114页

作者： WANG Jia Qi CHEN Xiao Li ZHOU Gui Lan WANG Hai Rui GU Yi Xin ZHANG Jian Zhong SHAO Zhu Jun ZHANG Mao Jun State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China National Institute of Environmental Health Chinese Center for Disease Control and PreventionBeijing 100021China

Objective To determine the distribution of two important virulence factors[lipooligosaccharide(LOS)and capsular polysaccharide(CPS)]in campylobacter jejuni(C.jejuni)isolated from different sources in China and to develop a rapid screening method for Guillain–Barrésyndrome(GBS)-associated strains.Methods Whole-genome sequencing was carried out for 494 C.jejuni strains.The Ortho MCL software was used to define the LOS/CPS gene clusters.CPS genotyping was performed with serotype-specific sequence alignment using the BLAST software.Real-time Polymerase chain reaction(PCR)was developed with the unique sequences of specific CPS types.Results Nine novel and 29 previously confirmed LOS classes were identified.LOS classes A,B,and C were the most common(48.2%,238/494)among the 494 strains.Twenty-six capsular types were identified in 448 strains.HS2,HS4c,HS5/31,HS19,and HS8/17 were the most frequent CPS genotypes(58.7%,263/448).Strains of 17 CPS genotypes(strain number>5)had one or two prevalent LOS classes(Pjejuni strains in China.The multiplex real-time PCR developed in this study will facilitate enhanced surveillance of GBS-associated strains in China.

关键词： campylobacter jejuni Lipooligosaccharide Capsular polysaccharide Multiplex real-time PCR

Genetic and Phenotypic Variation of campylobacter jejuni NCTC11168 Caused by flhA Mutation during Laboratory Passage

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Biomedical and Environmental Sciences 2023年第7期36卷 604-613页

作者： CHEN Xiao Li LIANG Hao GUO Peng Bo GU Yi Xin WANG Jia Qi WANG Hai Rui ZHOU Gui Lan SHAO Zhu Jun ZHANG Jian Zhong ZHANG Mao Jun State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Department of Microbiology School of Public HealthCheeloo College of MedicineShandong UniversityJinan 250100ShandongChina Shandong First Medical University Shandong Academy of Medical SciencesJinan 250000ShandongChina

Objective campylobacter jejuni NCTC11168 is commonly used as a standard strain for flagellar biosynthesis research.In this report,two distinguished phenotypic isolates(CJ1Z,flhA mutant strain,lawn;CJ2S,flhA complemented strain,normal colony)appeared during laboratory passages for NCTC11168.Methods Phenotypic assessments,including motility plates,transmission electron microscopy,biofilm formation assay,autoagglutination assay,and genome re-sequencing for these two isolates(CJ1Z,flhA mutant strain;CJ2S,flhA complemented strain)were carried out in this study.Results Transmission electron microscopy revealed that the flagellum was lost in CJ1Z.Phenotypic assessments and genome sequencing of the two isolates were performed in this study.The capacity for biofilm formation,colony auto-agglutination,and isolate motility was reduced in the mutant CJ1Z.Comparative genomic analysis indicated a unique native nucleotide insertion in flhA(nt,2154)that caused the I719Y and I720Y mutations and early truncation in flhA.Conclusion FlhA has been found to influence the expression of flagella in C.jejuni.To the best of our knowledge,this is the first study to describe the function of the C-terminal of this protein.

关键词： campylobacter jejuni Phenotypic variation Comparative genomics flhA

In Vitro Protein Expression Profile of campylobacter jejuni Strain NCTC11168 by Two-dimensional Gel Electrophoresis and Mass Spectrometry

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Biomedical and Environmental Sciences 2013年第1期26卷 48-53页

作者： ZHANG Mao Jun GU Yi Xin DI Xiao ZHAO Fei YOU Yuan Hai MENG Fan Liang ZHANG Jian Zhong Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and Prevention

Objective To investigate the protein expression profiles of the major food‐borne pathogen campylobacter jejuni NCTC11168.Methods Membrane and soluble cellular proteins were extracted from the genome‐sequenced C.jejuni strain NCTC11168.Protein expression profiles were determined using two‐dimensional gel electrophoresis（2‐DE）.All the detected spots on the 2‐DE map were subjected to matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry（MALDI‐TOF/TOF） analysis.Results A total of 537 and 333 spots were detected from the whole cell and membrane‐associated proteins of C.jejuni NCTC11168 cultured on Columbia agar medium at 42 ℃ by 2‐DE and Coomassie Brilliant Blue staining,respectively.Analyses of whole cell and membrane‐associated proteins included 399 and 133 spots,respectively,which included 182 and 53 functional proteins identified by MALDI‐TOF/TOF analysis.Conclusion The comprehensive expression protein profiles of C.jeuni NCTC11168 obtained in this study will be useful for elucidating the roles of these proteins in further pathogenesis investigation.

关键词： campylobacter jejuni Two‐dimensional gel electrophoresis MALDI‐TOF Soluble cellular protein Membrane protein

Rapid detection of campylobacter jejuni using fluorescent microspheres as label for immunochromatographic strip test

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Rapid detection of Campylobacter jejuni using fluorescent mi...

A campylobacteriosis Outbreak Caused by One Asymptomatic Food Handler Carrier

第八届全国免疫学学术大会

作者： Di Xu Lijun Wang Xing Ming Tingtao Chen Feng Xu Hua Wei Nanchang University

campylobacter jejuni is a worldwide foodborne pathogen recognized as a leading cause of human gastrointestinal enteritis.A rapid, sensitive, and specific method is required to monitor food and water in cases of contamination by this pathogen.This report presents a novel immunochromatographic test(ICT) using fluorescent microspheres labeled with specific polyclonal antibodies of C.jejuni as the capture reagent dispensed onto the conjugate pad.Polyclonal antibodies against the outer membrane protein PEB1 of C.jejuni were used as the detective reagent at the test line, whereas the goat anti-rabbit Ig G was used on the control line.PEB1 was obtained by gene cloning and expression to prepare its antibody.In this study, for the first time we report a simple and rapid ICT for detecting C.jejuni with a detection limit of 106 CFU/ml.

关键词： campylobacter jejuni protein PEB1 fluorescent microsphere polyclonal antibodies immunochromatographic strip test

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生物医学与环境科学:英文版 2023年第8期36卷 779-782页

作者： YAN Ai Xia ZHOU Gui Lan GAO Peng KANG Ying LI Shou Fei WANG Yuan Yuan WANG Feng Shuang WANG Hai Rui LI Ying ZHANG Mao Jun Shunyi District Center for Disease Control and Prevention Beijing 101300China State Key Laboratory Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China

In August 2021,three students with diarrhea from the same school visited a local hospital in the S district of Beijing.An epidemic investigation showed that there were more students with diarrhea in the same school and they had one meal together.campylobacter jejuni was isolated from both patients with diarrhea and asymptomatic food handlers;however,the latter also carried campylobacter coli.Phylogenomic analysis showed that there was a campylobacteriosis outbreak among the students,and the asymptomatic food handler may have been the source of the infection.Routine inspection and surveillance for campylobacter is needed for the food producing staff,particularly those cooking in the cafeteria in schools or other public food services.

关键词： campylobacter jejuni Outbreak Whole genome sequence Asymptomatic food handler

Effect of dairy Probiotics production on flaA 28 promoter in campylobacter jejuni

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Effect of dairy Probiotics production on flaA 28 promoter in...

Antibacterial Activity and Mechanism of Action of Zinc Oxide Nanoparticles against campylobacter jejuni

中国食品科学技术学会第五届年会暨第四届东西方食品业高层...

作者： Ding Wu1 Koul Liping Wang2 Haifeng (1.College of Food Science &Engineering,Northwest A&F University,Yangling,Shaanxi 712100,China 2.Canadian Research Institute for Food Safety,University of Guelph.ON.Canada NIG 2W1

＜正＞Lactobacilli and Bifidobacteria are important members of the gastrointestinal microflora of humans and animals and are thought to have positive effects on human health.Therefore,there is an increasing interest in using these microorganisms as probiotics,either in fermented dairy products or formulated as tablets.However,convincing scientific data to support claims of their health benefits are scarce.Study the inhibition of germfree extracts derived form milk fermented by lactic acid bacteria （LAB）on virulence gene flaA expression of foodborne pathogen bacteria campylobacter jejuni.A luminescent phenotype fusion between the flaA σ28 promoter and a promoterless luxCDABE cassette carried on the plasmid pRYluxCDABE in campylobacter jejuni was used.Though evaluation the bioluminescence to assess the effect of germfree extracts derived form milk fermented by lactic acid bacteria（LAB）（5 Bifidobacterium strains and 6 Lactobacillus strains）on flaA σ28promoter activity. The results show that gremfree extracts of milk fermented by LAB were found to have an significant（p 0.05）inhibitive effect on the expression of flaA σ28promoter,down-regulate virulence gene flaA expression.

关键词： campylobacter jejuni flaA σ28 promoter luminescence probiotics

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Antibacterial Activity and Mechanism of Action of Zinc Oxide...

Genetic and Antibiotic Resistance Characteristics ofcampylobacterjejuni Isolated from Diarrheal Patients,Poultry and Cattle in Shenzhen

中国食品科学技术学会第八届年会暨第六届东西方食品业高层...

作者： Peter L.Irwin Tony Jin School of Agriculture ＆ Biology Shanghai Jiao Tong University

＜正＞The antibacterial effect of zinc oxide（ZnO ）nanoparticles on campylobacter jejuni was investigated for inhibition and inactivation of cell growth.The results showed that C.jejuni was extremely sensitive to treatment with ZnO nanoparticles.The MIC of ZnO nanoparticles for C.jejuni was determined to be 0.05 to 0.025 mg/ml,which is 8- to 16-fold lower than that for Salmonella enterica serovar Enteritidis and Escherichia coli O157:H7（0.4 mg/ml）.The action of ZnO nanoparticles against C.jejuni was determined to be bactericidal,not bacteriostatic.Scanning electron microscopy examination revealed that the majority of the cells transformed from spiral shapes into coccoid forms after exposure to 0.5 mg/ml of ZnO nanoparticles for 16 h,which is consistent with the morphological changes of C.jejuni under other stress conditions. These coccoid cells were found by ethidium monoazide-quantitative PCR（EMA-qPCR）to have a certain level of membrane leakage.To address the molecular basis of ZnO nanoparticle action,a large set of genes involved in cell stress response,motility,pathogenesis,and toxin production were selected for a gene expression study.Reverse transcription-quantitative PCR（RT-qPCR）showed that in response to treatment with ZnO nanoparticles,the expression levels of two oxidative stress genes（katA and ahpC）and a general stress response gene（dnaK）were increased 52-,7-,and 17-fold,respectively.These results suggest that the antibacterial mechanism of ZnO nanoparticles is most likely due to disruption of the cell membrane and oxidative stress in campylobacter.

关键词： ZnO nanoparticles campylobacter jejuni antibacterial mechanism oxidative stress gene expression

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Biomedical and Environmental Sciences 2018年第8期31卷 579-585页

作者： JU Chang Yan ZHANG Mao Jun MA Yan Ping LU Jing Rang YU Mu Hua CHEN Hui LIU Chu Yun GU Yi Xin FU Yan Yan DUAN Yong Xiang Nanshan Center for Disease Control and Prevention Shenzhen 518054GuangdongChina State Key Laboratory of Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment ofInfectious DiseasesNational Institute for Communicable Disease Control and PreventionChinese Center for DiseaseControl and PreventionBeijing 102206China ORD Environmental Protection AgencyCincinnatiOhio 45220United States

Objective To investigate genetic and antibiotic resistance characteristics of campylobacter jejuni(C. jejuni) isolated from Shenzhen. Methods Multilocs sequence typing and agar dilution methods were used to define the genotype and antibiotic resistance of C. jejuni, respectively. Results In total, 126 C. jejuni strains were isolated. The prevalence of C. jejuni was 5.3% in diarrheal patients. The prevalence in poultry meat(36.5%) was higher than that in cattle meat(1.1%). However, the prevalence in poultry cloacal swabs(27.0%) was lower than that in cattle stool(57.3%). Sixty-two sequence types were obtained, among which 27 of the STs and 10 alleles were previously unreported. The most frequently observed clonal complexes were ST-21(11.9%), ST-22(10.3%), and ST-403(7.1%). ST-21, ST-45, ST-354, ST-403, and ST-443 complexes overlapped between isolates from patients and cattle, whereas ST-45 and ST-574 complexes overlapped between isolates from patients and poultry. All C. jejuni were resistant to at least one antibiotic. The highest resistance rate was toward ciprofloxacin(89.7%), followed by tetracycline(74.6%), and nalidixic acid(69.0%). Conclusion This is the first report of the genotypes and antibiotic resistance of C. jejuni in Shenzhen. Overlapping clonal complexes were found between isolates from patients and cattle, and between patients and poultry.

关键词： campylobacter jejuni Multilocus sequence typing Antibiotic resistance Poultry Cattle Diarrheal patients

A Case-control Study on Children with Guillain-barre Syndrome in North China

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Biomedical and Environmental Sciences 2003年第2期16卷 105-111页

作者： GAI-FEN LIU ZHENG-LAI WU HU-SHENG WU QUAN-YI WANG GE-TU ZHAO-RI CHUN-YONG WANG ZHI-XUE LIANG SHU-LAN CUI JIAN-DE ZHENG CorrespondenceshouldbeaddressedtoGai-FenLIU PekingUnionMedicalCollegeandChineseAcademyofMedicalSciencesBeijing100005China

To explore the risk factors for Guillain-barre syndrome. Methods Case-control study design was used in 51 cases of Guillain-barre syndrome,and 51 matched controls.All of the 51 cases in this study had been examined by electrophysiology. Serum IgG antibodies specific for C. jejuni were determined in all the subjects by ELISA. Each case and control were interviewed using an ad hoc questionnaire, including his/her demographic information,onset of the illness, their personal hygiene and so on. Results The study showed that Guillain-barre syndrome was associated with a few factors, such as polio vaccine immunization before onset of illness (OR=7.27), no hand washing after defecation and before meals (OR=6.15). Infection of C. jejuni was strongly associated with the illness (OR=9.5,Pjejuni and poor personal hygiene in children.

关键词： Guillain-Barre syndrome (GBS) Case-control study campylobacter jejuni

HLA alleles in patients with Guillain Barre syndrome

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Chinese Medical Journal 2000年第5期 45-48页

作者：李海峰袁锦楣郝洪军阎征王申五

To determine whether genetic factors may work in concert with autoimmune factors in the pathogenesis of Guillain Barre syndrome (GBS) Methods We determined the differences in the distribution of HLA alleles between GBS patients and normal controls HLA DQA, DQB and DRB alleles were typed by the sequence specific primer polymerase chain reaction (PCR SSP) methods in 47 GBS, 7 patients with campylobacter jejuni (CJ) enteritis and 50 normal controls Results There were no differences in the frequency of HLA DQA, DQB and DRB among GBS group, CJ group, normal controls and GBS subgroups with respect to recent CJ infection, GM1 IgG and GM1 IgM antibodies ( P >0 05) There was an increasing tendency of DQA1*0301 ( P =0 056, RR =2 991) in the subgroup with GM1 IgG antibodies and DQA1 *0302 ( P =0 087, RR =3 587) in the subgroup with recent CJ infection No difference was found among GBS, CJ enteritis patients and normal controls Conclusions The increasing tendency of the two alleles suggests that there may be some relation between genetic factors and immunological factors, but a definite conclusion waits for more cases

关键词： Guillain Barre syndrome human leukocyte antigens polymerase chain reaction genotyping campylobacter jejuni GM1