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Quantitation of camellianin a in HepG2 cells using a high performance liquid chromatography-electrospray ionization tandem mass spectrometric method

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Chinese Journal of Natural Medicines 2017年第3期15卷 234-240页

作者： CHEN Qian-Qian XI Hai-Ling WANG Cai-Yun XU Feng-Guo ZHANG Wei State Key Laboratory of Quality Research in Chinese Medicine Macao University of Science and Technology School of Public Health Baotou Medical College Key Laboratory of Drug Quality Control and Pharmacovigilance(Ministry of Education) China Pharmaceutical University

The present study was designed to develop a sensitive and selective high performance liquid chromatography-tandem mass spectrometric method for the determination of camellianin a in HepG2 *** extraction of camellianin a was achieved using 15% trichloroacetic acid and then separated on a C_(18) column interfaced with a triple quadrupole tandem mass spectrometer in multiple reaction monitoring *** mobile phase was consisted of methanol-water(0.1% formic acid)(55:45,V/V).The total run time was 5.0 *** method was linear in the concentration range of 0.25-250.0 ng·mL^(-1).The lower limit of quantification was 0.25 ng·mL^(-1).The intra-and inter-day relative standard deviations of entire concentration range were less than 9.3%.The proposed HPLC-MS/MS method was successfully applied to detect the intracellular concentration of camellianin a in HepG2 cells.

关键词： camellianin a HepG2 cells HPLC-MS/MS

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Roles of Adinandra nitida(Theaceae) and camellianin a in HCl/ethanol-induced acute gastric ulcer in mice

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Food Science and Human Wellness 2022年第4期11卷 1053-1063页

作者： Erdong Yuan Yingyi Lian Qiuhua Li Zhaoxiang Lai Lingli Sun Xingfei Lai Ruohong Chen Shuai Wen Junquan Zhu Wenji Zhang Shili Sun School of Food Science and Engineering South China University of TechnologyGuangzhou 510641China Tea Research Institute Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation&UtilizationGuangzhou 510640China Guangdong Society of Plant Protection Guangdong 510640China

Gastric ulcer is a global health concern nowadays. Adinandra nitida, known as Shibi tea, is a flavonoidrich plant found in South China. A. nitida possesses many healthy properties, such as antioxidation and reducing blood pressure. However, its effects on gastric ulcer have not been investigated. This study aimed to investigate the effects of the Shibi tea water extract(STE) and its main flavonoid camellianin a(CA) in hydrochloric acid(HCl) and ethanol(Et OH)-induced acute gastric ulcer in mice. Administration of CA and STE for continuous two days after stimulation by HCl/Et OH significantly attenuated the deterioration of gastric mucosal damage by lowering the gross gastric mucosal index, histopathological injury index, the oxidative stress, the expression of the inflammatory cytokines TNF-α and IL-6, and the expression of inflammatory mediators i NOS and COX-2. Western blotting and immunohistochemistry analysis showed that CA and STE regulated the inflammatory signaling pathway protein levels of IκB-α and NF-κB. Taken together, our study verified that CA and STE have antioxidative and anti-inflammatory effects in gastric ulcer mice. We propose that A. nitida should be developed as natural functional food for acute gastric ulcer patients base on the gastroprotective effects of STE and its main flavonoid CA.

关键词： Adinandra nitida(Theaceae) camellianin a Gastric ulcer Oxidative stress Anti-inflammation

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