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Horticultural Plant Journal 2024年第1期10卷 273-287页

作者： Wei Huang Danni Ma Fawad Zaman Xulei Hao Li Xia E Zhang Pu Wang Mingle Wang Fei Guo Yu Wang Dejiang Ni Hua Zhao National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanHubei 430070China

In plants,the lysine and histidine transporter(LHT)family represent a class of proteins that mediate the uptake,translocation,and utilization of amino acids.The tea plant(camellia sinensis)is a perennial evergreen with a relatively high level of amino acids.However,systematic identification and molecular characterization of the LHT gene family has rarely been reported in tea plants.In this study,22 CsLHTs were identified from the‘Shuchazao’genome and classified into two groups.The modeled three-dimensional structure and the conserved domains presented a high similarity among the LHTs proteins.Moreover,it was predicted that a few genes were conserved through the analysis of the physiochemical characters,structures and cis-elements in promoters.The expression patterns in tea plants revealed that CsLHT7 was mainly expressed in the roots,and CsLHT4 and CsLHT11 exhibited relatively high expression in both the roots and leaves.Moreover,the expression of all three genes could be induced by organic nitrogen.Additionally,heterogeneous expression of CsLHT4,CsLHT7 and CsLHT11 in Arabidopsis thaliana decreased the aerial parts biomass compared with that in WT plants while significantly increased the rosette biomass only for CsLHT11transgenic plants versus WT plants.Overall,our results provide fundamental information about CsLHTs and potential genes in N utilization for further analysis in tea plants.

关键词： camellia sinensis Nitrogen Lysine and histidine transporter(LHT)family

Analysis of NPK in camellia sinensis, Gliricidia sepium, and Musa acuminata Biomasses for Preparation of an Organic Fertilizers Formula for Young Tea Plants (Musa acuminata) and Studying of Their Nutrient Release Capacity in the Biodegradation Process

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Journal of Agricultural Chemistry and Environment 2024年第1期13卷 115-132页

作者： Madushan Dhammika Gunarathna Ashan Sithija Wickramaarachchi Polegodage Dilushi Sureka Ruwan Kumari Hiti Mudiyanselage Sithara Dilrukshi Wijekoon Deeyagahage Sujeewa Mallik de Silva Department of Chemistry Faculty of Science University of Kelaniya Kelaniya Sri Lanka Department of Microbiology Faculty of Science University of Kelaniya Kelaniya Sri Lanka Department of Statistics and Computer Science University of Kelaniya Kelaniya Sri Lanka

This study investigates the nitrogen (N), phosphorous (P), and potassium (K) contents in raw biomasses of camellia sinensis, Gliricidia sepium, and Musa acuminata. Therein, the highest N and P content was seen in camellia sinensis 116.80 ± 0.08 mg and 66.00 ± 0.14 mg respectively. The highest K content (106.80 ± 0.04 mg) was observed in Musa acuminata. Next, all three types of plant materials were allowed to decompose in water for 3 weeks, and a sample from each was analyzed for NPK after the 1st, 2nd, and 3rd week during decomposition. A significant increase in the release of N, P, and K by the camellia sinensis to water (P Musa acuminate were not significantly changed (P > 0.05) over time. The ratio for N:P:K was calculated for raw biomass samples and decomposed samples to find the best fitting N:P:K ratio to apply to young tea plants as organic fertilizers. In addition to that, the microbial insight of these organic compounds was analyzed by observing how microbial population increased with decomposition by the enumeration of the total microbial count. A considerable increment in total microbial count was observed up to 3.28 × 106, 1.21 × 1010, 2.18 × 108, and 6.49 × 107 CFU/ml for camellia sinensis, Gliricidia sepium, Musa accuminata (leaves), and Musa accuminata (trunk) respectively. The presence of phosphate solubilizing bacteria (PSB) and nitrogen solubilizing bacteria (NSB) throughout the decomposition period was confirmed by their growth on NBRIP and a modified nutrient medium that was specifically designed for the identification of ammonifiers respectively. Prepared fertilizer samples were applied to young tea plants that were grown in the Mawanella area in Sri Lanka (7°15'12.42"N 80°26'47.62"E) and according to the results, it is clear that fertilizer mixture 1 (N:P:K, 10:5:10, tea dust + Gliricidia + banana trunk) and fertilizer mixture 2 (N:P:K, 10:5:10, tea dust + Gliricidia + banana leaves) has the potential to increase the growth of young tea plants.

关键词： camellia sinensis Musa accuminata Gliricidia sepium Phosphate Solubilizing Bacteria Nitrogen Solubilizing Bacteria

Natural variation of main biochemical components,morphological and yield traits among a panel of 87 tea [camellia sinensis(L.) O. Kuntze] cultivars

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Horticultural Plant Journal 2023年第3期9卷 563-576页

作者： Fawad Zaman EZhang Li Xia Xielong Deng Muhammad Ilyas Ahmad Ali Fei Guo Pu Wang Mingle Wang Yu Wang Dejiang Ni Hua Zhao Key Laboratory of Horticultural Plant Biology of Ministry of Education Huazhong Agricultural UniversityWuhanHubei 430070China College of Horticulture and Forestry Sciences Huazhong Agricultural UniversityWuhanHubei 430070China College of Plant Science and Technology Huazhong Agricultural UniversityWuhanHubei 430070China

Many attentions have been previously focused to identify the multiple biochemical components related to tea quality and health benefits,however, the natural variation of biochemical components present in tea germplasm has not been adequately evaluated. In this study, the main biochemical components, leaf morphological and yield characteristics were evaluated for four rounds of tea leaves in a panel of 87 elite tea cultivars suitable for black, green, or oolong tea. Significant variations were observed among the tea cultivars, as well as seasonal differences in the levels of the free amino acid(FAA), caffeine(CAF), tea polyphenols(TP), water extract(WE) and TP to FAA ratio(TP/FAA). Results showed that the average levels of FAA showed a seasonal change, with the highest level of 4.0% in the 1st spring tea in the cultivars suitable for green tea and the lowest of 3.2% in summer tea in the cultivars suitable for black tea. The average CAF content was highest 3.2% in the cultivars suitable for oolong tea in the 1st spring and the lowest 2.5% in the cultivars suitable for green tea in summer. Limited seasonal and varietal variations were noticed in the average levels of WE among the three categories of tea. In addition, significant natural variation of the morphological characteristics, bud length varying from 2.5 cm to 8.7 cm, bud density from 190.3 buds · m-2to 1 730.3 buds · m-2, mature leaves biomass from 128.4 kg · hm-2to 2 888.4 kg · hm-2, and yield component traits of 100 buds(one bud with two leaves) dry weight from 3.7 g to37.7 g, tea yield/round from 444.6 kg · hm-2to 905.3 kg · hm-2, were observed. The aim of our evaluation was not only to identify the advantages of seasonal and clonal variations but also to provide a new viewpoint for their further application. Representative accessions were selected from the germplasm to promote the establishment of an inherent biochemical constituent expressing the quality of black, green, and oolong tea. The findings might be utilized to establish early selection criteri

关键词： camellia sinensis Biochemical components Germplasm Natural variation Morphology Yield

Differential metabolites and their transcriptional regulation in seven major tea cultivars (camellia sinensis) in China

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Journal of Integrative Agriculture 2023年第11期22卷 3346-3363页

作者： GAO Ting HOU Bing-hao SHAO Shu-xian XU Meng-ting ZHENG Yu-cheng JIN Shan WANG Peng-jie YE Nai-xing College of Horticulture Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in Universities of Fujian ProvinceFuzhou 350002P.R.China College of Horticulture Northwest A&F UniversityShaanxi 712100P.R.China

Various genetic and biochemical characteristics exist in tea plant cultivars,and they largely determine production suitability and tea quality.Here,we performed transcriptomic and metabolomic analyses of young shoots of seven tea cultivars and identified major regulatory transcription factors(TFs)for the characteristic metabolites in different cultivars based on weighted gene co-expression network analysis(WGCNA).Phenotypically,we found that‘Tieguanyin’(TGY)and‘Fujian Shuixian’(FJSX),which are suitable for oolong tea,had higher catechin contents.The metabolites of‘Jinxuan’(JX)were more prominent,especially the contents of phenolic acids,flavonoids,terpenes,and tannins,which were higher than those of the other six cultivars.Moreover,‘Fudingdabai’(FDDB),which is suitable for white tea,was rich in amino acids,linolenic acid,and saccharides.At the molecular level,hydroxycinnamoyl CoA quinate hydroxycinnamoyl transferase(HCT)(CsTGY12G0001876,and CsTGY06G0003042)led to the accumulation of chlorogenic acid in TGY.The main reason for the higher l-ascorbic acid content in FJSX was the high expression levels of L-galactono-1,4-lactone hydrogenase(GalLDH)(CsTGY13G0000389)and Myo-inositol oxygenase(MIOX)(CsTGY14G0001769,and CsTGY14G0001770),which were regulated by WRKY(CsTGY11G0001197).Furthermore,FDDB,‘Longjing 43’(LJ43),‘Shuchazao’(SCZ)and‘Baihaozao’(BHZ)had higher free fatty acid contents,among which MYB(CsTGY14G0002344)may be a hub gene for the regulation of palmitoleic acid accumulation.More importantly,we found that the shoots of TGY were green with purple,mainly due to the accumulation of anthocyanins and the downregulation of the Mg-protoporphyrin IX nonomethyl ester cyclase(MPEC)(CsTGY10G0001989)gene that affects chlorophyll synthesis.These results will provide a theoretical reference for tea cultivar breeding and suitability.

关键词： camellia sinensis transcriptomics metabolomics WGCNA

Functional mechanism on stem cells by tea(camellia sinensis)bioactive compounds

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Food Science and Human Wellness 2022年第3期11卷 579-586页

作者： Yao Cheng Jiachen Sun Hui Zhao Hongxing Guo Jianying Li School of Biotechnology and Food Science Tianjin University of CommerceTianjin 300134China The Third Central Clinical College Tianjin Medical UniversityTianjin 300170China

camellia sinensis(tea),one of the most popular commercial crops,is commonly applied in all parts of the world.The main active ingredients of tea include polyphenols,alkaloids,polysaccharides,amino acids,aroma and volatile constitutes,all of which are potentially responsible for the activities of tea.Stem cells(SCs)are the immature and undifferentiated cells by a varying capacity for proliferation,self-renewal and the capability to differentiate into one or more different derivatives with specialized function or maintain their stem cell phenotype.Herein,a thorough review is conducted of the functional mechanism on SCs by tea bioactive compounds.

关键词： Functional mechanism Stem cell camellia sinensis Bioactive compounds

Differentially Expression of Tual,a Tubulin-encoding Gene,during Flowering of Tea Plant camellia sinensis (L.) O.Kuntze Using cDNA Amplified Fragment Length Polymorphism Technique

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Acta Biochimica et Biophysica Sinica 2006年第9期38卷 653-662页

作者： Wan-Ping FANG~(1,2) Chang-Jun JIANG~1* Mei YU~1 Ai-Hua YE~1 Zhao-Xia WANG~(1,3)1 Key Laboratory of Tea Biochemistry and Biotechnology,Ministry of Agriculture,Anhui Agricultural University,Hefei 230036,China 2 College Of Horticulture,Nanjing Agriculture University,Nanjing 210095,China 3 Auhui Institute of Education,Hefei 230036,China Key Laboratory of Tea Biochemistry and Biotechnology Ministry of Agriculture Anhui Agricultural UniversityHefei 230036 China College Of Horticulture Nanjing Agriculture UniversityNanjing 210095 China Auhui Institute of EducationHefei 230036 China

The complementary DNA （cDNA） amplified fragment length polymorphism technique wasused to isolate transcript-derived fragments corresponding to genes involved in the flowering of tea plant.Comparative sequence analysis of an approximately 300 bp differential fragment amplified by primer combi-nation E11M11 revealed 80%-84% similarity to the corresponding part of an α-tubulin gene of other species.The complete cDNA sequence of this α-tubulin was cloned by the rapid amplification of cDNA ends technique;its full length is 1537 bp and contains an open reading frame of 450 amino acid residues with two N-glycosylation sites and four protein kinase C phosphorylation sites.The deduced amino acid sequences didshow significant homology to the α-tubulin from other plants that has been reported to be a pollen-specificprotein and could be correlated with plant cytoplasm-nucleus-interacted male sterility.We named this completecDNA Tual.The nucleotide and amino acid sequence data of Tual have been recorded in the GenBanksequence database with the accession No.DQ340766.This Tual gene was cloned into the pET-32aexpression system and expressed in Escherichia coli BL21trxB（DE3）.The molecular weight of expressedprotein was deduced to be approximately 49 kDa.Western blot analysis was used to identify the temporalexpression of Tual in tea plant.Further studies of the effect of Tual protein on pollen tube growth indicatedthe Tual solution obviously promoted the growth of tea pollen tube.

关键词： cDNA-AFLP camellia sinensis flower bud α-tubulin Western blot pollen

JA-mediated MYC2/LOX/AOS feedback loop regulates osmotic stress response in tea plant

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Horticultural Plant Journal 2024年第3期10卷 931-946页

作者： Junyan Zhu Hongrong Chen Lu Liu Xiaobo Xia Xiaomei Yan Xiaozeng Mi Shengrui Liu Chaoling Wei State Key Laboratory of Tea Plant Biology and Utilization/Key Laboratory of Tea Biology and Tea Processing of Ministry of Agriculture/Anhui Provincial Laboratory of Tea Plant Biology and Utilization Anhui Agricultural UniversityHefeiAnhui 230036China

Osmotic stress caused by low-temperature,drought and salinity was a prevalent abiotic stress in plant that severely inhibited plant development and agricultural yield,particularly in tea plant.Jasmonic acid(JA)is an important phytohormone involving in plant stress.However,underlying molecular mechanisms of JA modulated osmotic stress response remains unclear.In this study,high concentration of mannitol induced JA accumulation and increase of peroxidase activity in tea plant.Integrated transcriptome mined a JA signaling master,MYC2 transcription factor is shown as a hub regulator that induced by mannitol,expression of which positively correlated with JA biosynthetic genes(LOX and AOS)and peroxidase genes(PER).CsMYC2 was determined as a nuclei-localized transcription activator,furthermore,ProteinDNA interaction analysis indicated that CsMYC2 was positive regulator that activated the transcription of CsLOX7,CsAOS2,CsPER1 and CsPER3via bound with their promoters,respectively.Suppression of CsMYC2 expression resulted in a reduced JA content and peroxidase activity and osmotic stress tolerance of tea plant.Overexpression of CsMYC2 in Arabidopsis improved JA content,peroxidase activity and plants tolerance against mannitol stress.Together,we proposed a positive feedback loop mediated by CsMYC2,CsLOX7 and CsAOS2 which constituted to increase the tolerance of osmotic stress through fine-tuning the accumulation of JA levels and increase of POD activity in tea plant.

关键词： camellia sinensis Jasmonic acid(JA) MYC2 transcription factor Lipoxygenase(LOX) Osmotic stress Peroxidase(POD) Reactive oxygen species(ROS)

Identification of miRNAs and their targets in tea (camellia sinensis)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第10期14卷 916-923页

作者： Quan-wu ZHU Yao-ping LUO Institute of Tea Science Zhejiang University

MicroRNAs(miRNAs) are endogenous small RNAs playing a crucial role in plant growth and development,as well as stress responses.Among them,some are highly evolutionally conserved in the plant kingdom,this provide a powerful strategy for identifying miRNAs in a new species.Tea(camellia sinensis) is one of the most important commercial beverage crops in the world,but only a limited number of miRNAs have been identified.In the present study,a total of 14 new C.sinensis miRNAs were identified by expressed sequence tag(EST) analysis from 47 452 available C.sinensis ESTs.These miRNAs potentially target 51 mRNAs,which can act as transcription factors,and participate in stress response,transmembrane transport,and signal transduction.Analysis of gene ontology(GO),based on these targets,suggested that 37 biological processes were involved,such as oxidation-reduction process,stress response,and transport.Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis inferred that the identified miRNAs took part in 13 metabolic networks.Our study will help further understanding of the essential roles of miRNAs in C.sinensis growth and development,and stress response.

关键词： MicroRNA(miRNA) camellia sinensis Tea Gene ontology Pathway

Effect of drying methods and parameters on the antioxidant properties of tea(camellia sinensis)leaves

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食物生产加工与营养(英文)

食物生产加工与营养(英文) 2020年第1期2卷 72-82页

作者： A.S.Roslan A.Ismail Y.Ando A.Azlan Department of Nutrition and Dietetics Faculty of Medicine and Health SciencesUniversiti Putra Malaysia43400 SerdangSelangorMalaysia Department of Biological Functions Engineering Graduate School of Life Science and Systems EngineeringKyushu Institute of Technology2-4 HibikinoWakamatsu-kuKitakyushuFukuoka 808-0196Japan

Background:Conventional drying using heated air oven is commonly used as a method for preserving the product but often affects the nutritional value,taste,and texture.However,the heat from the drying method can oxidize and destroy heat-sensitive compounds.Superheated steam(SHS)drying uses superheated steam instead of hot air or combustion gases in a direct dryer and was reported better at preserving the nutritional values of food products.Aim:To evaluate the effect of SHS drying on antioxidant properties of tea leaves.The study also compared SHS drying with conventional and freeze-drying methods.Results:Tea leaves dried using freeze drying retained the highest level of antioxidant properties compared to other drying methods.The leaves dried using SHS exhibited significantly higher radical scavenging activity,ORAC and FRAP values compared to oven drying method.At different drying temperatures(150℃and 175℃),oven dried leaves showed significantly higher(p<0.05)antioxidant properties than that of SHS dried ones.Tea leaves dried for 60,75,and 90 min using SHS showed significantly higher(p<0.05)FRAP and ORAC values,and also total phenolic content compared to oven dried tea leaves.Conclusion:Tea leaves dried using SHS drying method retained higher level of antioxidant properties compared to oven drying.The drying method also retained lower antioxidant properties as drying time increased.Further study involving SHS drying in food-related fields should be conducted to support its usefulness.

关键词： camellia sinensis Superheated steam drying Antioxidant capacity Drying method

Structural,morphological,and optical properties of tin(Ⅳ) oxide nanoparticles synthesized using camellia sinensis extract: a green approach

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International Journal of Minerals,Metallurgy and Materials 2017年第9期24卷 1043-1051页

作者： J.Celina Selvakumari M.Ahila M.Malligavathy D.Pathinettam Padiyan Department of Physics Manonmaniam Sundaranar UniversityTirunelveli 627012Tamil NaduIndia

Tin oxide(SnO) nanoparticles were cost-effectively synthesized using nontoxic chemicals and green tea(camellia sinensis) extract via a green synthesis method. The structural properties of the obtained nanoparticles were studied using X-ray diffraction, which indicated that the crystallite size was less than 20 nm. The particle size and morphology of the nanoparticles were analyzed using scanning electron microscopy and transmission electron microscopy. The morphological analysis revealed agglomerated spherical nanoparticles with sizes varying from 5 to 30 nm. The optical properties of the nanoparticles’ band gap were characterized using diffuse reflectance spectroscopy. The band gap was found to decrease with increasing annealing temperature. The O vacancy defects were analyzed using photoluminescence spectroscopy. The increase in the crystallite size, decreasing band gap, and the increasing intensities of the UV and visible emission peaks indicated that the green-synthesized SnOmay play future important roles in catalysis and optoelectronic devices.

关键词： tin oxide nanoparticles camellia sinensis band gap photoluminescence