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Parasitic nematodes of amphibians from Lombok Island,Indonesia with description of camallanus senaruensis *** Meteterakis lombokensis ***.

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Journal of Coastal Life Medicine 2016年第9期4卷 708-713页

作者： Endang Purwaningsih Kartika Dewi Herjuno Ari Nugroho Zoological Division of Research Center for Biology-Indonesian Institute of Sciences Widyasatwaloka BuildingJl.Raya Jakarta Bogor Km 46Cibinong 16911Indonesia

Objective:To determine and describe the nematode species of amphibians collected from Lombok Island,West Nusa Tenggara,***:The materials examined were found in the intestines of twenty-four Fejervarya cancrivora(***),sixteen Fejervarya verruculosa,six Duttaphyrnus melanostictus(***)from Senaru and Gangga District,Lombok Island on April *** amphibian hosts were collected by *** observing the nematodes,the hosts were anesthesized to death with *** ventral of the host was opened by longitudinal incision and the internal organs were removed,placed separately in the Petri-dish and then examined under a dissecting *** nematodes found were fixed with warm 70%*** specimens for light microscope observation were cleared in glycerol and mounted in the same solution,and for the SEM Jeol scanning electron microscope 5310 low vacuum were re-fixed in caccodylate buffer and glutaraldehyde,dehydrated through a graded series of alcohol and vacuum-dried using TAITEC Vacuum-96Nitrogen,prior to attaching to stubs with double sided cello-tape,coated with gold 400?thickness in an Eico I-B2 ion *** were made with the aid of a drawing tube attached to a Nikon compound *** were given in micrometers(μm)as the average,followed by the range in parentheses,unless otherwise ***:Two new species of nematodes were found and described herein:camallanus senaruensis ***.,and Meteterakis lombokensis ***.,parasitic in the intestine of *** and ***,*** differs from other congeners in having a bluntly rounded tip of tail in the male and female,the structure of trident and having teeth in the buccal *** lombokensis differs from other previously described species in having no vulval flap,has a strongly widened proximal end of spicules,forming a cup shaped,and the number of caudal *** species found are Meteterakis

关键词： Nematode camallanus senaruensis sp.nov. Meteterakis lombokensis sp.nov. Amphibian Lombok Island

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