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A single-cell transcriptome atlas reveals the trajectory of early cell fate transition during callus induction in Arabidopsis

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Plant Communications 2024年第8期5卷 18-31页

作者： Ruilian Yin Ruiying Chen Keke Xia Xun Xu College of Life Sciences University of Chinese Academy of SciencesBeijing 10049China BGI Research Beijing 102601China Guangdong Provincial Key Laboratory of Genome Read and Write BGI-ShenzhenShenzhen 518120GuangdongChina

The acquisition of pluripotent callus from somatic cells plays an important role in plant development studies and crop genetic *** developmental process incorporates a series of cell fate transitions and ***,our understanding of cell heterogeneity and mechanisms of cell fate transition during callus induction remains quite ***,we report a time-series single-cell transcriptome experiment on Arabidopsis root explants that were induced in callus induction medium for 0,1,and 4 days,and the construction of a detailed single-cell transcriptional atlas of the callus induction *** identify the cell types responsible for initiating the early callus:lateral root primordium-initiating(LRPI)-like cells and quiescent center(QC)-like ***-like cells are derived from xylem pole pericycle cells and are similar to lateral root *** delineate the developmental trajectory of the dedifferentiation of LRPI-like cells into QC-like ***-like cells are undifferentiated pluripotent acquired cells that appear in the early stages of callus formation and play a critical role in later callus development and organ *** also identify the transcription factors that regulate QC-like cells and the gene expression signatures that are related to cell fate ***,our cell-lineage transcriptome atlas for callus induction provides a distinct perspective on cell fate transitions during callus formation,significantly improving our understanding of callus formation.

关键词： callus induction single-cell RNA-seq cell fate transition developmental trajectory transcriptional regulation pluripotency

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Proline and Glutamine Improve in vitro callus induction and Subsequent Shooting in Rice

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Rice science 2015年第6期22卷 283-289页

作者： Bhausaheb PAWAR Prashant KALE Jyoti BAHURUPE Ashok JADHAV Anil KALE Sharad PAWAR State Level Biotechnology Centre Mahatma Phule Agricultural University

This study was conducted to evaluate the effects of proline and glutamine on in vitro callus induction and subsequent regeneration and to develop a reproducible and highly efficient plant regeneration protocol in four rice genotypes, viz. Pawana, Jaya, Indrayani and Ambemohar. Considerable variation in response to plant growth regulators and amino acid supplements used was observed in all the four genotypes. Medium supplemented with proline and glutamine was shown to be superior to medium without proline and glutamine. The best callusing from mature embryo was observed on Murashige and Skoog （MS） medium supplemented with 2.0 mg/L 2,4-dichlorophenoxyacetic acid （2,4-D）, 500 mg/L proline and 500 mg/L glutamine. Shoot induction was higher in the callus obtained from medium supplemented with 500 mg/L proline and 500 mg/L glutamine. The highest shoot regeneration frequency （83.2%） was observed on MS medium with 2.0 mg/L benzylaminopurine, 0.5 mg/L 1-naphthaleneacetic acid, 500 mg/L proline, and 500 mg/L glutamine in the callus obtained from MS medium supplemented with 2.0 mg/L 2,4-D, 500 mg/L proline and 500 mg/L glutamine. Among the four genotypes, Pawana has the highest regeneration efficiency （83.2%）, whereas the regeneration efficiency of the rest three rice genotypes was in the range of 32.0% to 72.3%. This optimized regeneration protocol can be efficiently used for Agrobacterium mediated genetic transformation in rice.

关键词： callus induction glutamine proline rice Agrobacterium mediated genetic transformation 2,4-dichlorophenoxyacetic acid Murashige and Skoog medium

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Validating field regeneration capacity for selected accessions of Gossypium hirsutum using callus induction and regeneration capacity

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Journal of Cotton Research 2023年第2期6卷 98-111页

作者： TAJO Sani Muhammad PAN Zhaoe HE Shoupu JIA Yinhua MAHMOOD Tahir NAZIR Mian Fasil HU Daowu WANG Liru SADAU Salisu Bello IBRAHIM Sani AUTA Umar GENG Xiaoli DU Xiongming Zhengzhou Research Base National Key Laboratory of Cotton Bio‑Breeding and Integrated UtilizationZhengzhou UniversityZhengzhou 450001China National Key Laboratory of Cotton Bio‑Breeding and Integrated Utilization Institute of Cotton ResearchChinese Academy of Agriculture SciencesAnyangChina Oil Crops Reaserch Institute Chinese Academy of Agriculture SciencesWuhanChina Bioresources Development Centre National Biotechnology Development AgencyAbujaNigeria

Background Gossypium hirsutum undergoes rapid clonal propagation to regenerate a mature plant through tissue ***,the correlation between cotton leaf regeneration,callus induction,and regeneration ability was still *** this research,cotton leaf regeneration level for 21 accessions in the field(new leaves)was observed after the first harvest,and a comparison between field regeneration level and callus induction with its regeneration capacity(new shoots and roots)for the same 21 accessions was carried *** traits,including plant height,leaf area,fresh leaf weight,dry leaf weight,the number of flowers and bolls,and biochemical(proline content)and physiological(chlorophyll and carotenoid content)traits during the flowering stage of 21 upland cotton accessions,were *** A significant correlation between physiological parameters and callus induction was *** induction and regeneration capacity of roots and shoots for hypocotyl,cotyledons,and shoot tip tissues were used to validate field leaf regeneration level after the first *** 24 showed significant leaf regeneration in the field and callus induction capacity through callus induction and *** We found a substantial relationship between field regeneration capability and callus induction with its regeneration capacity for the hypocotyl,cotyledons,and shoot *** results showed that ZS061,Lumian 378,Jimian 863,and ZS065 have the highest moisture retention capacity,while CCRI 24,Liaoyang Duomaomian,and Beizhe Gongshemian have the lowest moisture retention *** 24 has the highest leaf regeneration capacity in the field,while Beizhe Gongshemian has the lowest leaf regeneration *** our result provides a clue for checking the regeneration capacity through leaf regeneration level in the field.

关键词： Upland cotton Agronomic traits Biochemical traits Tissue culture callus induction Regeneration capacity

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callus induction of Young Leaf Coconut cv. MATAG with Combination of 2,4-Dichlorophenoxyacetic Acid (2,4-D), α-Naphthalene Acetic Acid (NAA) and Benzyl Amino Purin (BAP)

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Advances in Bioscience and Biotechnology 2022年第5期13卷 254-263页

作者： Zuraida Ab Rahman Sentoor Kumaran Govindasamy Ahmad Ngalim Nur Auni Syazalien Adlan Nur Najwa Arifah Basiron Ayu Nazreena Othman Biotechnology & Nanotechnology Research Centre Headquarters of Malaysian Agricultural Research and Development Institute Serdang Malaysia Crop Science and Soil Science Research Centre Headquarters of Malaysian Agricultural Research and Development Institute Serdang Malaysia Universiti Malaysia Pahang Gambang Campus Gambang Malaysia

This research was to study in vitro callus induction in Coconut cv MATAG from young leaf explants. Young leaf segments from mature coconut were cultured on Y3 medium supplemented with different concentrations of 2,4-D and a combination of NAA and BAP. Each of these plant growth regulators (PGR) gives different responses toward callus formation, the percentage of explants producing callus, the percentage of callus proliferation, and the morphology of callus. A series of different concentrations were used for 2,4-D (1, 5, 10, 20, 40, 60, 100 mg/L), NAA (1, 3, 5 mg/L) and BAP (1, 3, 5 mg/L) respectively. The range of days of callus formation using 2,4-D treatments is 7 - 12 months, while the 2,4-D combined with NAA is recorded at 2 - 5 months. Despite the variety of different months between these plant growth regulators for callus formation, the percentages of explants producing callus and callus proliferation are different. The highest percentage of explants producing callus (2.9%) was observed at 2,4-D (40 mg/mL), followed by 2.7% at 2,4-D (10.0 mg/mL) with NAA (1 mg/mL). At a concentration of 100 mg/mL of 2,4-D, the highest percentage of callus proliferation was found, as well.

关键词： callus induction Coconut NAA 2 4-D

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callus induction from leaves of different paulownia species and its plantlet regeneration

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Journal of Forestry Research 2001年第4期12卷 209-214,276页

作者：范国强翟晓巧翟翠娟毕会涛河南农业大学泡桐研究所郑州450002

The experiment was carried out on five different species of Paulownia for callus induction from leaves. MS medium was adopted as basic medium, and from different combinations of NAA and BA the suitable media were determined for callus induction, bud differentiation, and root differentiation of five different species. MS+0.5NAA+4BA, MS+0.3NAA+2BA, MS+0.5NAA+4BA, MS+0.3NAA+6BA, and MS+0.3NAA+8BA were suitable media of callus inductions of leaves, respectively, for Paulownia tomentosa, Paulownia australis, Paulownia fortunei, Paulownia elongata and P. tmentosa x P. fortunei, and MS+0.3NAA+12BA, MS+0.3NAA+12BA, MS+0.5NAA+12BA, MS+0.5NAA+12BA, and MS+0.7NAA+12BA were suitable media for bud differentiation from leaf callus respectively for above five species. The rooting media was determined as 2MS+0.1NAA, 1/2MS+0.1NAA, 1/2MS, 1/2MS+0.3NAA, and 1/2MS+0.5NAA. These results provide reference data for breeding new fine va-rieties with different kinds of Paulownia protoplasts fusions.

关键词： Paulownia callus induction Plantlet regeneration Medium Hormone

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Influences of Genotype and Explant on callus induction and Shoot Regeneration in Sunflower （Helianthus annuus L.）

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Journal of Agricultural Science and Technology 2011年第2期5卷 226-231页

作者： M. Bayraktaroglu N. Dagustu Department of Field Crops Faculty of Agriculture Uludag University Gorukle Bursa 16059 Turkey

In vitro regeneration was performed with the aim of developing efficient callus and shoot regeneration from different explants of sunflower （Helianthus annuus L.）. The 6 genotypes （RHAI0, RHA 14, RHA15, PR6404, N Record 109/Sanay 3-5, N Record 109/Sera） were used as plant materials. The roots, hypocotyls and cotyledons were excised from 4 day-old seedlings and cultured on embryo induction medium （EIM） supplemented with Benzylaminopurine （BAP）, Naphthaleneacetic acid （NAA） and Gibberellic acid （GA3）. The experiments were kept in 18/6 hour light/dark photoperiod at 26± 2 ℃for four weeks. The rates of callus and root organ formation on explants were 67%-100% and 7%-31% respectively, depending on the genotype. Root explants produced statistically high callus formation （2.6 score） compared to cotyledon （2.0 score） and hypocotyl explants （2.5 score） at all sunflower genotypes used. The highest shoot regeneration was obtained from RHAI 5 （7%） while PR6404 （100%） produced the highest callus formation.

关键词： Sunflower (Helianthus annuus L.) genotype explant callus induction shoots regeneration.

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Effects of Cultivar and Explant Sources on callus induction and Plant Regeneration in Rice （Oryza sativa L.）

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Journal of Agricultural Science and Technology(B) 2018年第2期8卷 97-104页

作者： Hasan Akay Orhan Kurt Field Crops Department Faculty of Agriculture Ondokuz Mayis University Samsun 55200 Turkey

In vitro callus induction and plant regeneration potentiality were studied from mature embryo of three Indian rice （Oryza sativa L.） groups at Field Crops Department, Agricultural Faculty, Ondokuz May？s University, Samsun, Turkey. The study was done by using callus induction MS medium having different concentration of four. The present research was conducted according to the design of randomized blocks trial. A total of 696 calluses, 193 plants and 917 seeds were obtained from Indica group; 2,110 calluses, 103 plants and 235 seeds were obtained from Japonica group; 1,243 calluses and 13 plants were obtained from Javanica group. With regard to number of calluses obtained from each explant source, 52 calluses were obtained from whole-plant explants, 1,668 calluses from root explants, 629 calluses from shoot explants, 649 calluses from the 1st node explants, 240 calluses from the 2nd node explants, 269 calluses from the 1st internode explants and 12 calluses from the 2nd internode explants. With regard to number of plants obtained from each explant source, 27 plants were obtained from whole-plant explants, 195 plants from shoot explants, 43 plants from the 1st node explants, 40 plants from the 2nd node explants and four plants from the 1st internode explants. With regard to number of seeds, 823 seeds were obtained from shoot explants and 329 seeds were obtained from the 2nd node explants. Germination rate of harvested seeds was over 90%. The establishment of this regeneration system is essential for the development of a genetic transformation system for commercial rice cultivars.

关键词： Rice Oryza sativa L. callus induction plant regeneration.

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Optimizing the callus induction protocol of Catharanthus roseus by uniform design and Proliferation

Optimizing the Callus induction protocol of Catharanthus ros...

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2009中国植物学会植物细胞生物学学术年会

作者： WU Chun-lian~(1*),FAN Jian-ming~2,YANG Jun~1,CHEN Wei-ying~1,SUN Min~3 1.School of Life Sciences,China West Normal University,Nanchong,Sichuan province 637002,China 2.The Laboratory of Toxicology,College of Public Health,Zhengzhou University, Zhengzhou 450001,China 3.School of Life Science,Southwest University,Chongqing 400715,China

Catharanthus roseus(L.) *** is one of the most extensively investigated medicinal plants and is known mainly for its pharmacologically important indole *** leaves of Catharanthus roseus which were used as *** parameters,two hormones and Vitamin C(Vc),with four concentrations,were designed for callus induction of Catharanthus roseus by uniform design(UD),and 8 experiments were *** results showed that MS medium with 2.0 mg·L 6-BA,1.0 mg·L 2,4-D and 20 mg·L Vc was the optimum medium for callus induction(100%).The optimum proliferation medium was MS+6-BA O.5 mg·L+2,4-D 0.5 mg·L+20 mg·L *** results indicated UD could be used to optimize conditions for callus induction of Catharanthus *** could inhibit browning of callus induction of Catharanthus roseus.

关键词： Catharanthus Roseus optimum culture medium uniform design callus induction

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Carbon Sources and Supporting Materials in callus induction Effects on Regeneration of Indica Rice(Oryza sativa ***6and RD15)

Carbon Sources and Supporting Materials in Callus Induction ...

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The 8th International Symposium on Biocontrol and Biotechnology

作者： Panutat Darachai Sutee Chutipaijit Kanokporn Sompornpailin College of Nanotechnology King Mongkut's Institute of Technology Ladkrabang

The effect of carbon source and supporting material compositions on callus induction and plant regeneration from mature seeds of two indica rice varieties(RD6 and RD15)was studied in the present ***1 medium was used for callus induction from the sterilized *** then,three-week-old calli were transferred to four media(NBSl,NBS 2,NBM1 and NBM2)which were different in carbon sources and supporting materials for 3 *** of RD6 and RD15 varieties derived from the NBM2 medium(30 g Lmaltose as a carbon source and 2 g Lagar+4 g LPhytagelas a supporting material)showed the higher frequency for green spots and shoot regenerations when compared to the *** spot frequency was 91.67%and83.33%in RD6 and RD15 varieties,***,calli obtained from the same medium also showed maximum shoot regenerations in RD6 and RD15 varieties(75.00%and 66.67%,respectively).Well-developed plantlets were hardened and transferred to the soil.

关键词： callus induction maltose RD6 RD15 regeneration

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callus induction from Mature Embryos of Maize

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Agricultural Science & Technology 2009年第6期10卷 67-68,93页

作者：肖莉杰王丽艳闵丽黑龙江八一农垦大学生命科学技术学院黑龙江大庆163319 黑龙江省农垦科研育种中心黑龙江哈尔滨150090

In this study we studied the factors influencing the callus induction from mature embryos of maize inbred lines Qi 319, Zhen 58, Chang 7 -2, Lx 9801 and 81162, such as genotype, combination of plant growth regulators, and low-temperature pretreatment. The results showed that the induction rate of Qi 319 was the highest among the four genotypes tested; combination of 4.0 mg/L 2,4-D ＋ 0.5 mg/L 6-BA was suitable for inducing callus from mature embryos; three days of 4℃ pretreatment can promote the callus induction significantly. The indices optimized in the present study are helpful for establishing genetic transformation system in maize without considering seasonal variation.

关键词： Maize Mature embryos callus induction

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