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Assessment of bone charcoal dusts of six species of mammal for the postharvest control of callosobruchus maculatus Fabricius(Coleoptera:Chrysomelidae)infestations of cowpea seeds:can the charcoal dust affect seed viability in laboratory and in field?

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Food Quality and Safety 2019年第4期3卷 279-287页

作者： Luke Chinaru Nwosu Department of Crop and Soil Science University of Port HarcourtPort HarcourtRivers StateNigeria

Objectives:The possibility that bone charcoal dusts of some species of mammal will control the major insect pest of stored cowpea seeds and the implications on seed viability(after pest control process)were investigated in the laboratory at ambient temperature(30±3°C)and relative humidity(70±5%)and in *** and Methods:Standard entomological and agronomical techniques were ***:The results showed that at 1.0%w/w dosage,the bone charcoal dusts of Ovis aries and Bos taurus were more effective than permethrin standard insecticide in killing adult callosobruchus maculatus *** cowpea seeds in *** taurus had a faster action speed than permethrin at 1.0%w/*** 1.5%w/w of dust,all the species of mammal investigated deterred oviposition considerably in *** females,whereas only the bone charcoal dusts of *** and Sus scrofa were comparable with permethrin in suppressing adult emergence and seed damage;*** only was comparable with permethrin in reducing larval *** charcoal dusts of *** and *** require chemical and olfactory analyses to know if they might have caused irritation to the insects and disrupted insect circadian rhythm,affecting behaviour and mating activities,*** bone charcoal dusts of the six species of mammal at a highest test dose of 1.5%w/w allowed high seed viability in both laboratory and ***:The study recommends the use of bone charcoal dusts of *** and *** at economical-and quality-favourable dose 1.5%w/w for managers seeking to control *** insect attacking cowpea seeds in *** transitive components of the bones,abrasion of the insect epicuticle lipid layer by the charcoal dust,and combustion-related toxic factors were responsible for the insecticidal activities of the bone charcoal dusts of the mammals.

关键词： bone charcoal dust mammal stored cowpea seed viability callosobruchus maculatus

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Insecticidal toxicity of two bruchid-resistant cowpea cultivar powders as cowpea seed protectants against callosobruchus maculatus(Fab.)(Coleoptera:Chrysomelidae)

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Food Quality and Safety 2019年第1期3卷 35-39页

作者： Kayode D.Ileke Department of Biology School of ScienceFederal University of TechnologyOndo StateNigeria

Toxicity of bruchid-resistant cultivars MIT04K-399-1 and MIT07K-299-92 powders as cowpea protectants against callosobruchus maculatus(Fab.)was investigated at an ambient temperature of 28±2°C and a relative humidity of 75±5%.Their efficacy was tested at dosage,0.5,1.0,1.5,2.0,2.5,and 3.0 g/20 g of cowpea seeds on adult mortality,oviposition,adult emergence,progeny development,seed damage,weight loss,and Beetle Perforation Index(BPI)by cowpea *** result showed that toxicity of the bruchid-resistant cowpea cultivar powders on adult bruchid is dosage-and *** a dosage of 3.0 g/20 g,cultivars MIT04K-399-1 and MIT07K-299-92 powders achieved 70%and 72.5%lethal effect on adult *** after 4 days of treatment,*** and percentage adult emergence of *** decrease with an increase in powder ***,reduction in progeny development of *** increases with an increase in powder *** mean number of adult emergence decreased at 2 g/20 g,2.5 g/20 g and 3 g/20 *** MIT04K-399-1 and MIT07K-299-92 powders significantly affected egg *** MIT07K-299-92 powder was more effective against oviposition and adult emergence at higher concentrations than MIT04K-399-1 powder,an effect that was not significantly different(P>0.05)from each *** BPI of 9.1 and 6.2 was recorded on cowpea seeds treated with MIT04K-399-1 and MIT07K-299-92 powders at a dosage of 3 g/20 g,*** fear of residual toxicity on nontargeted organisms is not associated with the use of resistant cowpea cultivars as seed *** farmers are familiar with the technology involved in the preparation and application of powders as *** of bruchid-resistant cowpea cultivars MIT04K-399-1 and MIT07K-299-92 powders into the management of coleopteran pests will ensure availability of viable seeds for future farming in developing nations such as Nigeria.

关键词： bruchid resistant cultivar MIT04K-399-1 cultivar MIT07K-299-92 callosobruchus maculatus Vignia unguiculata

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Evaluation of the dust and methanol extracts of Garcinia kolae for the control of callosobruchus maculatus (F.) and Sitophilus zeamais (Mots)

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第12期8卷 912-916页

作者： OGUNLEYE R.F. ADEFEMI S.O. Department of Zoology University of Ado-Ekiti Ado Ekiti Ekiti State 234 Nigeria Department of Chemistry University of Ado-Ekiti Ado Ekiti Ekiti State 234 Nigeria

Insecticidal effects of different doses of the dust and methanol extracts of Garcinia kolae on callosobruchus maculatus and Sitophilus zeamais were tested. The dust had no significant effect on the two insects, none of them died even at 3 d after treatment. The methanol extracts, however, had rapid lethal effects on both C. maculatus and S. zeamais. The mortality of C. maculatus by the lowest concentration of methanol extracts ranged from 95%-100% whereas in S. zeamais, the mortality ranged from 87.5%-100% and 70%-100% in concentrations of 1 g extract＋3 ml methanol and 1 g extract＋5 ml methanol, respectively, from 24 to 48 h. The least concentration of 1 g extract＋l 5 ml methanol had no significant lethal effect on Sitophilus zeamais.

关键词： Insecticidal effect Garcinia kolae Sitophilus zeamais callosobruchus maculatus

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Effects of Initial Infestation Levels of callosobruchus maculatus (F.)(Coleoptera: Chrysomelidae) on Cowpea and Use of Nicotiana tabacum *** Extract as Grain Protectant

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Journal of Northeast Agricultural University(English Edition) 2015年第4期22卷 1-6页

作者： Musa A K Odunayo A Adeyeye O E Department of Crop Protection University of Ilorin

This study determined the effects of initial infestation of cowpea seeds （Ife brown variety） with different insect densities （0, 2, 4 and 6 pairs per 50 g seeds） of callosobruchus maculatus （F.） and evaluated the effects of aqueous leaf extract of Nicotiana tabacum L. on C. maculatus in the laboratory. It was observed that adult beetle population increased significantly （p〈0.05） with increase in insect density. The increase in population of beetles and corresponding weight loss of the seeds in different levels of infestation showed that the cowpea variety was susceptible to beetle infestation, emergence and survival of progeny. Significantly more adults emerged on higher infestation compared to lower and no infestation. In Nigeria, Nicotiana tabacum L. is a locally available plant, with known insecticidal properties. The plant leaf extract was easily extracted with water and confirmed its effectiveness as a protective agent for stored cowpea seeds. Experiment was conducted to assess the effects of aqueous extracts ofN. tabacum at 0, 0.1, 0.2 and 0.3 mL＂ 50 g-1 cowpea seeds on C. maculatus. Data was recorded and showed varying levels of effectiveness against C. maculatus. Result showed that seed appearance was dependent on levels of insect population, while N. tabacum aqueous extract exerted effects on survival of C. maculatus. Aqueous leaf extract of N. tabacum probably contained some insecticidal properties which might have significantly conferred beetle mortality and reduced beetle emergence leading to a decrease in seed weight loss.

关键词： callosobruchus maculatus grain protectant infestation Nicotiana tabacum seed damage

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Combination of Piper guineense Essential Oil with Cowpea Varietal Resistance in Control of Cowpea Seed Beetle, callosobruchus maculatus Fabricius (Coleoptera： Chrysomelidae： Bruchinae)

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Journal of Northeast Agricultural University(English Edition) 2017年第3期24卷 10-18页

作者： Samuel Adelani Babarinde Ebenezer Oluwaseun Esan Olagoke Zacchaeus Olatunde David Seye Ajayi Jesusegun Paul Olaniyi Department of Crop and Environmental Protection Ladoke Akintola University of TechnologyP.M.B.400Ogbomoso 210001Nigeria Maize Improvement Program for Southern Ecology Institute of Agricultural Research and TrainingMoor PlantationIbadan 200262Nigeria Department of Pure and Applied Chemistry Ladoke Akintola University of TechnologyP.M.B.400Ogbomoso 210001Nigeria

To reduce the risks associated with over-dependence on synthetic insecticides and ensure leguminous food security, an experiment was conducted to evaluate the potentials of integrating Piper guineense essential oil （PgEO） with cowpea varietal resistance in the control of cowpea seed beetle, Callosobruehus maculatus Fabricius. Four cowpea varieties （Sokoto, Drum, Oloyin and Gwallam） were studied. Data collected included oviposition, progeny emergence, Reproductive Efficiency （RE） and Percentage Seed Damage （PSD）. At first filial generation, numbers of eggs laid on Oloyin variety treated with 25 μL PgEO per 25 g cowpea seeds （2.15） were significantly （p〈0.05） lower than the values obtained in untreated cowpea （3.61） and hexane-treated seeds （3.07）. C. maculatus RE on Oloyin （33.73） was significantly （p〈0.05） lower than values obtained on other varieties （47.34-54.67） for seeds without PgEO; and were significantly greater than 0.00 observed on Oloyin seeds treated with 50 pL PgEO. PSD on Oloyin （6.63%） was significantly （p〈0.05） lower than the values obtained on Sokoto （10.04%） and Drum （9.79%） for seeds without PgEO, while there was no damage in Oloyin treated with 50 pL PgEO. Significantly （p〈0.05） lower F2 progeny emergence occurred on Oloyin （4.02） and Sokoto （4.71） compared with Drum （9.03） and Gwallam （6.07） from the untreated cowpea seeds. The results implied that application of PgEO to Oloyin gave better protection from infestation of C. maculatus than other varieties. Hence, combination of varietal resistance with PgEO for management ofbruchid could be an effective mean of improving cowpea food security.

关键词： cowpea variety callosobruchus maculatus varietal resistance Piper guinensee filial generation integrated pest management

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Chemical Composition and Bioactivity of Lippia adoensis Hochst ***(Verneneaceae) Leaf Essential Oil Against callosobruchus maculatus Fabricius(Coleoptera: Chrysomelidae)

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Journal of Northeast Agricultural University(English Edition) 2016年第4期23卷 8-14页

作者： Babarinde Samuel Adelani Oladoye Sunday Olusegun Adesina Gabriel Olulakin Adebayo Moses Adeolu Department of Crop and Environmental Protection Ladoke Akintola University of Technology Department of Pure and Applied Chemistry Ladoke Akintola University of Technology Department of Crop Production and Soil Science Ladoke Akintola University of Technology

Essential oil （EO） of Nigeria-grown Lippia adoensis leaf was analyzed using gas chromatography mass spectrometry （GCMS） and its fumigant and repellent properties against callosobruchus maculatus were evaluated. Sixteen compounds predominated by monoterpenes were identified. The major compounds were Eucalyptol （28.36%）, α-Terpineol （25.99%）, γ-Terpinene （15.24%）, α-Pinene （5.08%）, 1H-Cyclopropa[a]naphthalene （4.25%） and 1, 3, 6, 10-Dodeeatetraene （3.74%）. Percentage mortality due to fumigant toxicity was dose- and exposure period-dependent. One hour after treatment （HAT）, application of L. adoensis leaf EO at 107 μL· L^-1 air caused significantly （p〈0.05） higher mortality （50.00%） than 0.00% mortality observed at 0-53 μL· L^-1 air, but not significantly （p〉0.05） different from 22.50% observed in 80 μL· L^-1 air. At 3 HAT, application ofL. adoensis EO at 80 μL· L^-1 air caused significantly higher mortality （90.00 %） than mortality observed at 0 μL· L^-1 air. At 6 HAT, application of L. adoensis EO at 53-107 μL· L^-1 air caused significantly higher mortality （100.00 %） than that was observed in the control. The same trend was observed at 12 HAT where 100 % mortality observed in 27-107 μL· L^-1 air was significantly greater than 13.33 % observed in the control. At 3 HAT, percentage repellence was significantly （p〈0.05） affected by doses. Application of EO at 10-30 μL· cm^2 caused class V repellence （86.67%-100%） compared with the control which caused class I repellence （0-20%）.

关键词： Lippia adoensis callosobruchus maculatus GCMS bioactivity repellence fumigant

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四纹豆象callosobruchus maculatus(F.)的种型及其防治

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粮食储藏 1988年第3期 3-9页

作者：孙宝根商业部四川粮食储藏科学研究所

四纹豆象是一种田间及储藏物豆类中重要害虫.在本文中论述四纹豆象生物学及其防治的6个主要方面.(1)四纹豆象种名的同义词.(2)四纹豆象与鹰咀豆象之间主要差异.(3)四纹豆象成虫的多型现象.(4)四纹豆象的标准型与活动型之间生物学特性.(5... 详细信息

四纹豆象是一种田间及储藏物豆类中重要害虫.在本文中论述四纹豆象生物学及其防治的6个主要方面.(1)四纹豆象种名的同义词.(2)四纹豆象与鹰咀豆象之间主要差异.(3)四纹豆象成虫的多型现象.(4)四纹豆象的标准型与活动型之间生物学特性.(5)活动型虫口的观察.(6)四纹豆象虫口的制止及防治.

关键词：四纹豆象成虫羽化豆类 callosobruchus maculatus 活动型种型

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Chemical Composition of Different Extracts of Conyza bonariensis: Insecticidal and Nematicidal Activities

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American Journal of Analytical Chemistry 2023年第2期14卷 95-120页

作者： Kodjo Adande Kodjo Eloh Oudjaniyobi Simalou Marie-France Bakaï Pierluigi Caboni Laboratoire de chimie organique et des substances naturelles (Lab COSNat) Département de Chimie Faculté Des Sciences Université de Lomé Lomé Togo Laboratoire de chimie organique et des Sciences de l’Environnement (LaCOSE) Département de Chimie Faculté Des Sciences et Techniques Université de Kara Kara Togo Department of Life and Environmental Sciences University of Cagliari Cagliari Italy

C. bonariensis (L.) Cronq. known as hairy fleabane was first described in Argentina but it is now widely spread through most warmer regions of Europe, Africa, Asia, the Caribbean and Central America. In this work, a chemical analysis by liquid and gas chromatography coupled with mass spectrometry of the whole plant, aerial part, flowers and roots extracts of C. bonariensis harvested in Togo (West Africa) was carried out. Two acetylenic compounds Lachnophyllum ester and limonene were identified as the main components of essential oils while Lachnophyllum and Matricaria lactones were dominant in chloroform extracts. Based on the plant chemical compositions, essential oils and chloroform extracts were tested on cowpea weevil callosobruchus maculatus adults which are considered as one of the most cosmopolitan pests of stored beans, and on freshly hatched second-stage juveniles of root-knot nematode Meloidogyne incognita. Results showed that the whole plant essential oil demonstrated an LC50/24h value of 1.75 μL oil/L air on C. maculatus while at 3.91 μL oil/L air, it showed 100% mortality. Furthermore, the plant root chloroform extracts partitioned in diethyl ether-hexane mixture showed the strongest nematicidal activity with an LC50/72h value of 0.47 mg/mL. Our findings suggest that the widely diffused plant C. bonariensis and its acetylenic constituents could be considered as potent botanical insecticidal and nematicidal agents.

关键词： Conyza bonariensis Insecticidal Activity Nematicidal Activity callosobruchus maculatus Meloidogyne incognita

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