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Predominant mucosal IL-8 mRNA expression in non-caga Thais is risk for gastric cancer

World Journal of Gastroenterology 2013年第19期19卷 2941-2949页

作者： Sirikan Yamada Shunji Kato Takeshi Matsuhisa Luksana Makonkawkeyoon Masaru Yoshida Thiraphat Chakrabandhu Nirush Lertprasertsuk Pawit Suttharat Bandhuphat Chakrabandhu Shin Nishiumi Wilaiwan Chongraksut Takeshi Azuma Division of Gastrointestinal Surgery and Endoscopy Department of SurgeryChiang Mai UniversityChiang Mai 50200Thailand Department of Surgery Nippon Medical SchoolSendagiBunkyo-kuTokyo 113-8603Japan Department of Gastroenterology and Endoscopy Tama-Nagayama HospitalNippon Medical SchoolNagayamaTama-shiTokyo 206-8512Japan Department of Biochemistry Faculty of MedicineChiang Mai UniversityChiang Mai 50200Thailand Department of Gastroenterology International Center for Medical Research and treatmentKobe University School of MedicineKobeHyogo 650-0017Japan Department of Pathology Faculty of MedicineChiang Mai UniversityChiang Mai 50200Thailand

AIM: To study gastric mucosal interleukine-8 (IL-8) mRNA expression, the cytotoxin-associated gene A (caga) mutation, and serum pepsinogen (PG)?I/II ratio related risk in Thai gastric cancer.METHODS: There were consent 134 Thai non-cancer volunteers who underwent endoscopic narrow band imaging examination, and 86 Thais advance gastric cancer patients who underwent endoscopic mucosal biopsies and gastric surgery. Tissue samples were taken by endoscopy with 3 points biopsies. The serum PG?I, II, and Helicobacter pylori (H. pylori) immunoglobulin G (IgG) antibody for H. pylori were tested by enzyme-linked immunosorbent assay technique. The histopathology description of gastric cancer and non-cancer with H. pylori detection was defined with modified Sydney Score System. Gastric mucosal tissue H. pylori DNA was extracted and genotyped for caga mutation. Tissue IL-8 and cyclooxygenase-2 (COX-2) mRNA expression were conducted by real time relative quantitation polymerase chain reaction. From 17 Japanese advance gastric cancer and 12 benign gastric tissue samples, all were tested for genetic expression with same methods as well as Thai gastric mucosal tissue samples. The multivariate analysis was used for the risk study. Correlation and standardized t-test were done for quantitative data, P value caga gene of 86.8 per cent in Thai gastric cancer although there are high yields of the East Asian type in the positive caga. The H. pylori infection prevalence in this study is reported by combined histopathology and H. pylori IgG antibody test with 77.1% and 97.4% of sensitivity and specificity, respectively. The serum PG?I/II ratio in gastric cancer is significantly lower than in the non-cancer group, P = 0.045. The serum PG?I/II ratio of less than 3.0 and IL-8 mRNA expression ≥ 100 or log10 ≥ 2 are significant cut off risk differences between Thai cancer and non-cancer, P = 0.03 and P < 0.001,

关键词： Gastric cancer caga mutation Interleukine-8 mRNA expression Helicobacter pylori Pepsinogen I/II ratio

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