检索结果-南通市图书馆

Cloning and expression of Sgcyp450-4from Siraitia grosvenorii

Acta Pharmaceutica Sinica B 2016年第6期6卷 614-622页

作者： Dongping Tu Xiaojun Ma Huan Zhao Changming Mo Qi Tang Liuping Wang Jie Huang Limei Pan Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical College Yunnan Branch Institute of Medicinal Plant Development Chinese Academy of Medical Sciences Guangxi Botanical Garden of Medicinal Plants Horticulture & Landscape College Hunan Agriculture University Guangxi University of Chinese Medicine

cyp450 plays an essential role in the development and growth of the fruits of Siraitia grosvenorii. However, little is known about the Sgcyp450-4 gene in S. grosvenorii. Here, based on transcriptome data, a full-length c DNA sequence of Sgcyp450-4 was cloned by reverse transcriptasepolymerase chain reaction(RT-PCR) and rapid-amplification of c DNA ends(RACE) strategies. Sgcyp450-4 is 1677 bp in length(Gen Bank accession No. AEM42985.1) and contains a complete open reading frame(ORF) of 1422 bp. The deduced protein was composed of 473 amino acids, the molecular weight is 54.01 kDa, the theoretical isoelectric point(PI) is 8.8, and the protein was predicted to possess cytochrome P450 domains. Sgcyp450-4 gene was highly expressed in root, diploid fruit and fruit treated with hormone and pollination. At 10 days after treatment with pollination and hormones, the expression of Sgcyp450-4had the highest level and then decreased over time, which was consistent with the development of fruits of S.Grosvenorii. Hormonal treatment could significantly induce the expression of Sgcyp450-4. These results provide a reference for regulation of fruit development and the use of parthenocarpy to generate seedless fruit, and provide a scientific basis for the production of growth regulator application agents.

关键词： Siraitia grosvenorii Auxin Sgcyp450-4 cyp87a3 Race clone Expression pattern

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：