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Monitoring the Expression of Maize Genes in Developing Kernels under Droug ht Stress usingOligo-microarray

Journal of Integrative Plant Biology 2010年第12期52卷 1059-1074页

作者： Meng Luo Jia Liu R. Dewey Lee Brian T. Scully Baozhu Guo The University of Georgia Department of Crop and Soil Sciences Tifton GA 31793 USA J. Craig Venter Institute Rockville MD 20850 USA US D-epartment of Agriculture Agricultural Research Service Crop Protection and Management Research Unit Tifton GA 31793 USA

Preharvest aflatoxin contamination of grain grown on the US southeastern Coast Plain is provoked and aggravated by abiotic stress. The primary abiotic stress is drought along with high temperatures. The objectives of the present study were to monitor gene expression in developing kernels in response to drought stress and to identify drought-responsive genes for possible use in germplasm assessment. The maize breeding line Tex6 was used, and gene expression profiles were analyzed in developing kernels under drought stress verses well-watered conditions at the stages of 25, 30, 35, 40, 45 d after pollination （DAP） using the 70 mer maize oligo-arrays. A total of 9 573 positive array spots were detected with unique gene IDs, and 7 988 were common in both stressed and well-watered samples. Expression patterns of some genes in several stress response-associated pathways, including abscisic acid, jasmonic acid and phenylalanine ammonia-lyase, were examined, and these specific genes were responsive to drought stress positively. Real-time quantitative polymerase chain reaction validated microarray expression data. The comparison between Tex6 and B73 revealed that there were significant differences in specific gene expression, patterns and levels. Several defense-related genes had been downregulated, even though some defense-related or drought responsive genes were upregulated at the later stages.

关键词： CORN GENETICS GENETIC regulation in plants EFFECT of drought on plants EFFECT of stress on plants DNA microarrays AFLATOXINS crops -- postharvest technology

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