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Expressions of cellular retinoic acid binding proteins i and retinoic acid receptor-β in the guinea pig eyes with experimental myopia

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international Journal of Ophthalmology(English edition) 2011年第2期4卷 131-136页

作者： Jia Huang Xiao-Mei Qu and Ren-Yuan Chu Department of Ophthalmology Eye and ENT Hospital Affiliated to Fudan University Shanghai 200031 China

OBJECTiVE:All-trans retinoic acid (RA) is the only extrinsic biochemical candidate known to date that could act as a growth controller, the aim of this study was to investigate the expression cellular retinoic acid binding proteins i (crabp-i) and retinoic acid receptor-β (RAR-β) in retina of the guinea pig eyes with experimental myopia.;METHODS:Ninety guinea pigs aged 14 days were equally and randomly divided into three groups: form deprivation (FD), -5D lens, and control. The diffusers for FD were white translucent hemispheres, and -5D lenses were used to introduce hyperopic defocus. Refraction was measured with streak retinoscopy after cycloplegia, and axial length was calculated with Cinescan A/B ultrasonography. Retina harvested at different time points were used to measure RA level with HPLC and expressions of cellular retinoic acid binding proteins i (crabp-i) and RA receptor-β (RAR-β) were assayed with Western blot and Real-time PCR. SPSS13.0 software was used for statistical analysis.;RESULTS:Up-regulations of crabp-i and RAR-β in ocular tissues correlated with changes in the refractive status and growth rate of the guinea pig eye (Pivation led to -5.14D myopia and a 0.281mm axial elongation; 14 days of monocular defocus produced -3.64D myopia and a 0.163 mm axial elongation. The level of retinal RA started to elevate in 7 days (Pisual manipulation in both FD and -5D lens groups and became more prominent by 14 days (Pions of crabp-i and RAR-β increased by 14 days after visual manipulation (Pincreased by 7 days after visual manipulation (Pich suggested that changes of expressions of crabp-i and RAR-β might lag behind the change of RA.;CONCLUSiONS:The levels of crabp-i and RAR-β were elevated in retina of the guinea pig eye with experimental myopia. During the progression of experimental myopia, the retinal RA level increased rapidly, and there might be a positive feedback between the

关键词： retinoic acid crabp-i RAR-β experimental myopia guinea pig

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