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Impact of Seed Halopriming on Germination,Morphological Traits,and Cry1Ac Gene Expression in Bt cotton(Gossypium hirsutum)

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Phyton-International Journal of Experimental Botany 2025年第1期94卷 229-241页

作者： Wenqi Shi Binyameen Bin Shafqat Muhammad Arif Ayesha Fazal Nawaz Muhammad Amir Zia Xu Ling Dingli Hong Ruhong Xu Luhua Li College of Agriculture Guizhou UniversityGuiyang550025China Faculty of Medicine and Health Technology Tampere UniversityTampere33720Finland Department of Life Sciences University of TriesteVia Licio Giorgieri 5Trieste34127Italy National Institute for Genomics and Advanced Biotechnology(NIGAB) National Agricultural Research Centre(NARC)Islamabad45500Pakistan

Seed priming is an effective seed pretreatment technology that enhances germination and overall crop performance by optimizing seed hydration and metabolic processes before *** quality is a critical determinant of cotton(Gossypium hirsutum)crop performance,influencing germination,plant vigor,and *** study evaluates the effects of seed priming with potassium salts(1%and 2%KCl and K2SO4)on germination,morphological traits,and Cry1Ac gene expression in three Bt cotton cultivars(IUB-2013,NIAB-878B,FH-142)as Cry1Ac enhance the pest resistance in Bt cotton and reduce the plant’s dependence on chemical *** were primed for six hours,air-dried,and sown in the *** rates,plant height,number of bolls per plant,boll weight,seed cotton yield,and ginning outturn(GOT)were assessed at crop ***1Ac gene expression was quantified to explore the influence of priming treatments on transgene *** demonstrated that 1%K2SO4 priming significantly enhanced germination and yield-related traits,with Cry1Ac expression peaking in the IUB-2013 cultivar under 1%K2SO4 *** findings suggest that potassium-based halopriming improves cotton seedling establishment and Bt gene *** study addresses the critical gaps in understanding the effects of seed halopriming on morphological traits,germination,and expression of the Cry1Ac gene in Bt cotton while providing a novel eco-friendly and cost-effective halopriming approach,offering the potential to improve cotton production.

关键词： cotton seed priming halopriming Cry1Ac Bt cotton germination yield traits

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cotton cultivation technology with Chinese characteristics has driven the 70-year development of cotton production in China

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Journal of Integrative Agriculture 2022年第3期21卷 597-609页

作者： FENG Lu CHI Bao-jie DONG He-zhong Zhengzhou Research Base State Key Laboratory of Cotton Biology School of Agricultural Sciences Zhengzhou University cotton Research Center Shandong Academy of Agricultural Sciences College of Agricultural Science and Technology Shandong Agriculture and Engineering University

Since the founding of the People’s Republic of China in 1949,significant achievements have been made in cotton production in *** has maintained its position as the world’s largest cotton producer for 33 years(1983–2015),with average annual increases of 3.5 and 3.9% in the unit yield and total output of cotton,*** production has played an extremely important role in the development of the national economy and the improvement of living *** the cotton planting area has been reduced in recent years,the total output has remained relatively unchanged due to the continuous increase in the unit ***’s dominant position in global cotton production is undoubtedly attributed to the progress and development of cotton cultivation *** the past 70 years,China has established a high-yielding and high-efficiency cotton cultivation mode that corresponds to its national conditions,including a large population and a limited land ***,cotton cultivation technology is constantly being innovated and developed to keep pace with the *** this paper,we review the development of cotton production and cultivation in China over the past 70 years,with a particular focus on the innovation and development of cotton cultivation technology with Chinese *** review is intended to provide guidance for the sustainable development of China’s cotton production in the future and to provide a reference for global cotton production.

关键词： China cotton cultivation technology outlook

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cotton GhBRC1 regulates branching,flowering,and growth by integrating multiple hormone pathways

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The Crop Journal 2022年第1期10卷 75-87页

作者： Quan Sun Yuanhui Xie Huimin Li Jinlei Liu Rui Geng Ping Wang Zongyan Chu Ying Chang Guanjun Li Xiao Zhang Youlu Yuan Yingfan Cai State Key Laboratory of cotton Biology Henan Key Laboratory of Plant Stress BiologySchool of Life SciencesHenan UniversityKaifeng 475000HenanChina Chongqing Key Laboratory of Big Data for Bio Intelligence College of BioinformationChongqing University of Posts and TelecommunicationsChongqing 400065China State Key Laboratory of cotton Biology Key Laboratory of Cotton Genetic ImprovementMinistry of AgricultureInstitute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang 455000HenanChina Kaifeng Academy of Agriculture and Forestry Kaifeng 475000HenanChina

cotton architecture is partly determined by shoot branching and flowering *** BRC1 was previously identified by RNA-seq analysis of nulliplex-branching and normal-branching ***,the roles of Gh BRC1 in cotton remain *** the present study,investigations of nuclear localization and transcriptional activity indicated that Gh BRC1 has characteristics typical of transcription *** expression analysis showed that Gh BRC1 was highly expressed in axillary buds but displayed different expression patterns between the two branching *** of Gh BRC1 in Arabidopsis significantly inhibited the number of branches and promoted *** contrast,silencing Gh BRC1 in cotton significantly promoted seedling *** BRC1 was induced by multiple hormones,including strigolactones,which promoted seedling growth and seed germination of Arabidopsis plants overexpressing Gh *** with these findings,RNA-seq analysis of virus-induced gene silencing treated cotton revealed that a large number of genes were differentially expressed between Gh BRC1-silenced and control plants,and these genes were significantly enriched in plant hormone signalling ***,our data indicates that Gh BRC1 regulates plant branching and flowering through multiple regulatory pathways,especially those regulating plant hormones,with functions partly differing from those of Arabidopsis *** results provide insights into the molecular mechanisms controlling plant architecture,which is important for breeding cotton with ideal plant architecture and high yield.

关键词： cotton GhBRC1 Branching Flowering Growth

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cotton ethylene response factor Gh ERF91 is involved in the defense against Verticillium dahliae

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Journal of Integrative Agriculture 2024年第10期23卷 3328-3342页

作者： Nurimanguli Aini Yuanlong Wu Zhenyuan Pan Yizan Ma Qiushuang An Guangling Shui Panxia Shao Dingyi Yang Hairong Lin Binghui Tang Xin Wei Chunyuan You Longfu Zhu Dawei Zhang Zhongxu Lin Xinhui Nie Key Laboratory of Oasis Eco-agriculture Xinjiang Production and Construction Group/Agricultural CollegeShihezi UniversityShihezi 832003China National Key Laboratory of Crop Genetic Improvement/College of Plant Sciences&Technology Huazhong AgriculturalUniversityWuhan 430070China cotton Research Institute Shihezi Academy of Agricultural ScienceShihezi 832011China Institute of Economic Crops Xinjiang Academy of Agricultural SciencesUrumqi 830091China

Verticillium dahliae causes significant losses in cotton *** reveal the mechanism of the defense response to *** in cotton,transcriptomic analyses were performed using cotton cultivars M138(***-resistant)and P2(***-susceptible).The results revealed 11,076 and 6,640 differentially expressed genes(DEGs)in response to ***,*** weighted gene co-expression network analysis of 4,633 transcription factors(TFs)indicated a“MEblue”module containing 654 TFs that strongly correlate with resistance to *** these TFs,the ethylene response factor Ghi_A05G10166(GhERF91)was identified as a putative hub gene with a defense response against ***.A virus-induced gene silencing assay and exogenous application of ethephon showed that GhERF91 is activated by ethylene and positively regulates the response to *** exposure in *** study provides fundamental transcriptome data and a putative causal gene(GhERF91)associated with resistance to ***,as well as genetic resources for breeding ***-resistant cotton.

关键词： cotton Verticillium wilt RNA-sequencing GhERF91 virus-induced gene silencing

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cotton maturity and responses to harvest aids following chemical topping with mepiquat chloride during bloom period

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Journal of Integrative Agriculture 2022年第9期21卷 2577-2587页

作者： QI Hai-kun DU Ming-wei MENG Lu XIE Liu-wei AEgrinya ENEJI XU Dong-yong TIAN Xiao-li LI Zhao-hu Engineering Research Center of Plant Growth Regulator Ministry of Education/College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193P.R.China Department of Soil Science Faculty of AgricultureForestry and Wildlife Resources ManagementUniversity of CalabarCalabar 540271Nigeria Hebei cottonseed Engineering Technology Research Center Hejian 062450P.R.China

Early maturity, complete defoliation and boll opening are essential for the efficient machine harvesting of cotton. Chemical topping, involving one extra application of mepiquat chloride(MC) in addition to its traditional multipleapplication strategy, may be able to replace manual topping. However, it is not known whether this chemical topping technique will influence maturity or cotton responses to harvest aids. In this 2-yr field study, we determined the effects of the timing of chemical topping using various rates of MC on boll opening percentage(BOP) before application of harvest aids(50% thidiazuron··ethephon suspension concentrate, referred to as TE), and the defoliation percentage(DP) and BOP 14 days after TE application. The results indicated that late chemical topping(near the physiological cutout, when the nodes above white flower is equal to 5.0) significantly decreased BOP before TE by 5.9–11.2% compared with early(at peak bloom) or middle(seven days after peak bloom) treatments in 2019, which was a relatively normal year based on crop condition. Also, a high MC rate(270 g ha) showed a significantly lower(22.0%) BOP before TE than low(90 g ha) or medium(180 g ha) rates. In 2020, which was characterized by stronger vegetative growth in the late season, the late chemical topping reduced the number of leaves before TE application relative to early or middle treatments, but had lower DP(23.2–27.2%) 14 days after TE application. The high MC rate showed a leaf count before TE application that was similar to the low and medium rates, but it showed the most leaves after TE and much lower(15.0–21.7%) DP in 2020. These results suggest that late timing of chemical topping and a high MC rate decreased the sensitivity of leaves to harvest aids. Further analysis indicated that the late chemical topping mainly affected the leaf drop from the mainstem and fruiting branches where the late regrowth occurred, and the high MC rate reduced leaf shedding from these parts and

关键词： cotton mepiquat chloride thidiazuron ethephon defoliation maturity

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cotton pixel identification with CBERS-02 CCD data based on spectral knowledge

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Science China(Technological Sciences) 2005年第S2期48卷 129-144页

作者： LI Jing1,2,3, LIU Qinhuo1,3, LIU Qiang1, CHEN Liangfu1, BO Junhua3 & LI Shaokun3 1. State Key Laboratory of Remote Sensing Science, IRSA, Chinese Academy of Sciences, Beijing 100101, China 2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China 3. The Key Oasis Eco-agriculture Laboratory of Xinjiang Production and Construction Corps, Shi Hezi 832000, China 1. State Key Laboratory of Remote Sensing Science IRSA Chinese Academy of Sciences 100101 Beijing China 2. Graduate University of Chinese Academy of Sciences 100049 Beijing China 3. The Key Oasis Eco-agriculture Laboratory of Xinjiang Production and Construction Corps 832000 Shi Hezi China

In this paper the in-situ experiment data are collected from the Spectral Library to estimate the possible values of the primary structural parameters in each cotton growing season by statistics. Based on these values the spectra of the cotton major growing seasons were simulated and analyzed using the canopy reflectance model, SAILH. In this way we also simulated the cotton pixel spectra corresponding to CBERS-02 CCD and took them as the reference spectra for spectra fitting. Then No.143 Regiment of No.8 Agricultural Division of Xinjiang Production and Construction Corps (XPCC) was chosen as the study area, and two spectra fitting methods, Mahalanobis distance and spectra angle, were used to identify cotton pixel from CBERS-02 CCD image of the study area. At last we analyzed the effect of the calibration coefficients error on cotton identifying. The results showed that within 4% error the spectral angle classifier can still have a good performance.

关键词： CBERS-02 cotton pixel identification spectra fitting.

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Quantification of the effects of management factors on maize (Zea mays L.) and cotton(Gossypium hirsutum L.) residues decomposition rate

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Journal of Environmental Sciences 2002年第2期14卷 165-172页

作者： JONES Chilima, HUANG Chang yong, WU Ci fang (College of Natural Resources and Environment, Zhejiang University, Hangzhou 310029, China. College of Natural Resources and Environment Zhejiang University Hangzhou 310029 China

Efforts to quantify management effects on decomposition rate of added substrates to the soil is important especially where such information is to be used for prediction in mathematical or simulation models. Using data from a short term (60 days) greenhouse simulation study, a procedure for quantifying effects of management on SOM and substrate decomposition is presented. Using microbial growth rate u (q), microbial efficiency in substrate utilization e (q), specific decomposition rates for added plant residues to two contrasting soils, red earth (Ferrasol) and black earth (Acrisol) were estimated. The treatments included straw addition + buried, (T1); straw addition + mineral N (T2); and straw addition + tillage, (T3). Sampling was done every 15 days. Straw decomposition rate was affected by external mineral N sources (Urea 46% N). Addition of an external N source significantly increased decomposition rates. The study could not, however, fully account for the effect of tillage on residues because of the limited effect of the tillage method due to the artificial barrier to mechanical interference supplied by the mesh bags. It is concluded that using few decomposer parameters, decomposition rates and consequently SOM trends in a soil system can be monitored and quantification of the influence of perturbations on decomposition rate of added substrates possible.

关键词： management factors residue decomposition rate maize cotton red soil black soil

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A Novel Intelligent System for Dynamic Observation of cotton Verticillium Wilt

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Plant Phenomics 2023年第1期5卷 73-84页

作者： Chenglong Huang Zhongfu Zhang Xiaojun Zhang Li Jiang Xiangdong Hua Junli Ye Wanneng Yang Peng Song Longfu Zhu College of Engineering Huazhong Agricultural UniversityWuhan 430070PR China College of Plant Science&Technology Huazhong Agricultural UniversityWuhan 430070PR China National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research(Wuhan)Huazhong Agricultural UniversityWuhan 430070PR China

Verticillium wilt is one of the most critical cotton diseases,which is widely distributed in cotton-producing ***,the conventional method of verticillium wilt investigation is still manual,which has the disadvantages of subjectivity and low *** this research,an intelligent vision-based system was proposed to dynamically observe cotton verticillium wilt with high accuracy and high ***,a 3-coordinate motion platform was designed with the movement range 6,100 mm×950 mm×500 mm,and a specific control unit was adopted to achieve accurate movement and automatic ***,the verticillium wilt recognition was established based on 6 deep learning models,in which the VarifocalNet(VFNet)model had the best performance with a mean average precision(mAP)of ***,deformable convolution,deformable region of interest pooling,and soft non-maximum suppression optimization methods were adopted to improve VFNet,and the mAP of the VFNet-Improved model improved by 1.8%.The precision–recall curves showed that VFNet-Improved was superior to VFNet for each category and had a better improvement effect on the ill leaf category than fine *** regression results showed that the system measurement based on VFNet-Improved achieved high consistency with manual ***,the user software was designed based on VFNet-Improved,and the dynamic observation results proved that this system was able to accurately investigate cotton verticillium wilt and quantify the prevalence rate of different resistant *** conclusion,this study has demonstrated a novel intelligent system for the dynamic observation of cotton verticillium wilt on the seedbed,which provides a feasible and effective tool for cotton breeding and disease resistance research.

关键词： system category cotton

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Preparation of superhydrophobic CdS cotton using visible light response and its application for the control of water pollution

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Science China(Technological Sciences) 2019年第12期62卷 2236-2242页

作者： GE Bo REN GuiNa ZHAO PengFei JIN ChuanYu LI WenZhi ZHANG ZhaoZhu School of Materials Science and Engineering Liaocheng UniversityLiaocheng 252000China School of Environmental and Material Engineering Yantai UniversityYantai 264405China State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China

Sewage discharge produced by homes and industries contains both oil and water-soluble components. In order to remove oil from water, materials with specific wettability can be used;however, these materials cannot remove dissolved contaminants from water. In this research, we prepared a superhydrophobic Cd S cotton for water purification. This material has the ability to adsorb different types of oil due to its non-wettability(WCA = 152°). Moreover, its response behavior towards visible light enables it to remove water-soluble pollutants as well. The highest oil absorption rate of superhydrophobic Cd S cotton is nearly 30 times, and it can absorb light below 564 nm and produce ·OH to degrade water soluble pollutants. Finally, based on Mott-Schottky curve,the position of Fermi energy level was determined(–0.55 e V). Also, the positions of valence and conduction bands were calculated based on the analysis of band gap which helps in proving photoelectric response characteristics.

关键词： superhydrophobic cotton CdS photocatalytic

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Enhanced antibacterial activity of cotton via silver nanocapsules deposited by atmospheric pressure plasma jet

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Plasma Science and Technology 2023年第3期25卷 130-137页

作者：张潇漫马晓萍李茂洋季佩宇黄天源诸葛兰剑吴雪梅 School of Physical Science and Technology Soochow UniversitySuzhou 215006People’s Republic of China Collaborative Innovation Center of Suzhou Nano Science and Technology Soochow UniversitySuzhou 215006People’s Republic of China The Key Laboratory of Thin Films of Jiangsu Soochow UniversitySuzhou 215006People’s Republic of China Analysis and Testing Center Soochow UniversitySuzhou 215123People’s Republic of China

In this work,the antibacterial activity of cotton containing silver nanocapsules prepared by atmospheric pressure plasma(APP)deposition is *** nanocapsules consist of a shell and a silver nanoparticle(Ag NP)core,where the core is used to bring antibacterial activity,and the shell is utilized to suppress the potential toxicity of Ag *** surface morphology and the elements of the samples are analyzed by scanning electron microscopy(SEM),energy dispersive x-ray and x-ray photoelectron spectroscopy(XPS).The SEM results show that the skin of the cotton fibers will fall off gradually after APP treatment over 3 min,and the XPS results show that the Ag content will rise to 1.6%after APP deposition for 10 ***,the antimicrobial activity tests show that the reduction rates of Escherichia coli and Staphylococcus aureus can achieve 100%when the sample is treated for 10 min,which exhibits excellent antibacterial *** addition,the UV absorption properties of the cotton will also be correspondingly improved,which brings a broader application prospect for antibacterial cotton.

关键词： atmospheric pressure plasma(APP) nanocapsules antimicrobial activity cotton

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