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Association of aphid life stages using DNA sequences： A case study of tribe Eriosomatini （Hemiptera： Aphididae Pemphiginae）

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Insect Science 2008年第6期15卷 545-551页

作者： He-Cai Zhang Dong Zhang Ge-Xia Qiao Institute of Zoology Chinese Academy of Sciences Beijing Henan Normal University Xinxiang Henan Province Graduate University of the Chinese Academy of Sciences Beijing China

Association of aphid life stages based on a portion of coi sequence was applied in Eriosomatini. Three aphid specimens, nos. 17496, 19265a, and 19265b collected on Gramineae roots all clustered with Tetraneura chinensis Mordvilko with strong support. The average pairwise p-distance among the four taxa was 0.001 （range, 0.000-0.002）, and that among all the ingroup taxa was 0.065 （range, 0.000-0.141）. This indicated that nos. 17496 and 19265 b were the secondary-host morphs of T. chinensis. In this paper the secondary host morph of T. chinensis is described for the first time. With the identification of more species＇ secondary-host morphs, aphids identification based on this morph will be made easily.

关键词： coi sequence Eriosomatini primary host morph secondary host morph Tetraneura chinensis

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A New Record of Ponyfish Deveximentum megalolepis(Perciformes: Leiognathidae) in Beibu Gulf of China

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Journal of Ocean University of China 2017年第3期16卷 468-472页

作者： JU Yuman SONG Na CHEN Guobao SUN Dianrong HAN Zhiqiang GAO Tianxiang Fisheries College Ocean University of China South China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences School of Fishery Zhejiang Ocean University

A new record ponyfish, Deveximentum megalolepis Mochizuki and Hayashi, 1989, was documented based on its morphological characteristics and DNA barcode. Fifty specimens were collected from Beibu Gulf of China and identified as D. megalolepis by morphological characterization. The coloration, meristic traits, and morphometric measurements were consistent with previously published records. In general, it is a silver-white, laterally compressed and deep bodied ponyfish with 6–9 rows of scales on cheek; scale rows above lateral line 6–8; scale rows below lateral line 14–17. Mitochondrial cytochrome oxidase I subunit(coi) gene fragment was sequenced for phylogenetic analysis. There is no sequence variation of coi gene between the specimens collected in this study. The genetic distances between D. megalolepis and other congeneric species range from 3.6% to 14.0%, which were greater than the threshold for fish species delimitation. The coi sequence analysis also supported the validity of D. megalolepis at genetic level. However, the genetic distance between Chinese and Philippine individuals was about 1.2% and they formed two lineages in gene tree, which may be caused by the geographical distance.

关键词： Deveximentum megalolepis Beibu Gulf new record coi sequence

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