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Epidemiological trends of mosquito-borne viral diseases in Pakistan

Animal Diseases 2022年第2期2卷 127-136页

作者： Muhammad Imran Jing Ye Muhammad K.Saleemi Iqra Shaheen Ali Zohaib Zheng Chen Shengbo Cao State Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityWuhan 430070HubeiChina Key Laboratory of Preventive Veterinary Medicine in Hubei Province College of Veterinary MedicineHuazhong Agricultural UniversityWuhan 430070HubeiChina The Cooperative Innovation Center for Sustainable Pig Production Huazhong Agricultural UniversityWuhan 430070HubeiChina Department of Pathology Faculty of Veterinary ScienceUniversity of AgricultureFaisalabad 38040Pakistan Livestock and Dairy Development Department PunjabPakistan Department of Microbiology Faculty of Veterinary&Animal SciencesThe Islamia University of BahawalpurBahawalpur 63100Pakistan Department of Preventive Veterinary Medicine College of Animal Science and TechnologyJiangxi Agricultural UniversityNanchang 330045JiangxiChina

Globally,arboviruses are public health *** has seen a fast-paced increase in mosquito-borne Flavivirus diseases such as dengue because of deforestation,climate change,urbanization,poor sanitation and natural *** magnitude and distribution of these diseases are poorly understood due to the lack of a competitive nationwide surveillance *** dengue-endemic countries,the recent epidemics of chikungunya(chikv)and human West Nile virus(WNV)have created panic among the public and are thought to provoke an outbreak of Zika virus(ZIKV)in ***,hospital-based surveillance has indicated the presence of Japanese encephalitis virus(JEV),which is deeply concerned by developing countries such as *** situation could become more devastating because of poorly developed diagnostic *** date,no licensed vaccine has been used in Pakistan,and preventive measures are mainly based on vector *** review provides comprehensive information concerning the association of risk factors with disease occurrence,epidemiological trends,and prediction of the spread of mosquito-borne diseases,attention to new threats of ZIKV,and future perspectives by benchmarking global health policies.

关键词： Arboviruses Dengue chikv JEV WNV ZIKA Public health

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Chikungunya virus,epidemiology,clinics and phylogenesis:A review

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Asian Pacific Journal of Tropical Medicine 2014年第12期7卷 925-932页

作者： Alessandra Lo Presti Alessia Lai Eleonora Cella Gianguglielmo Zehender Massimo Ciccozzi Department of Infectious Parasitic and Immunomediated Diseases Epidemiology UnitReference Centre on Phytogeny Molecular Epidemiology and Microbial Evolution(FEMEM).Istituto Superiore di Sanita RomeItaly Department of Biomedical and Clinical Sciences L Sacco HospitalUniversity of MilanMilanItaly University Campus-Biomedico RomeItaly

Chikungunva virus is a mosquito-transmitted alphavirus that causes chikungunva fever,a febrile illness associated with severe arthralgia and *** virus is transmitted by culicine mosquitoes:Chikungunya virus replicates in the skin,disseminates to liver,muscle,*** tissue and brain,presumably through the *** studies showed that the Indian Ocean and the Indian subcontinent epidemics were caused by two different introductions of distinct strains of East/Central/South African genotype of *** paraphyletic grouping of African CHIK viruses supports the historical evidence that the virus was introduced into Asia from *** analysis divided Chikungunva virus isolates into three distinct genotypes based on geographical origins:the first,the West Africa genotype,consisted of isolates from Senegal and Nigeria;the second contained strains from East/Central/South African genotype,while the third contained solely *** most recent common ancestor for the recent epidemic,which ravaged Indian Ocean islands and Indian subcontinent in 2004- *** found to date in *** lineage dated about 1952 and exhibits similar spread patterns of the recent Indian Ocean outbreak lineage,with successive epidemics detected along an eastward *** group splitted into two clades:an Indian lineage and a south east *** of Chikungunya virus fever in Asia have not been associated necessarily with outbreaks in *** tools can reconstruct geographic spread of Chikungunva virus during the epidemics *** good management of patients with acute Chikungunva virus infection is essential for public health in susceptible areas with current Aedes spp activity.

关键词： chikv Epidemiology Phylogeny

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Determination of Chikungunya Virus RNA by Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay

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Advances in Microbiology 2022年第7期12卷 398-414页

作者： Barnali Sen Sabrina Afrin Sharmin Zahan Saif Ullah Munshi Sifat Uz Zaman Department of Virology IEDCR Dhaka Bangladesh Department of Microbiology Popular Medical College Dhaka Bangladesh Department of Community Medicine Mugda Medical College Dhaka Bangladesh Department of Virology BSMMU Dhaka Bangladesh Infection Prevention and Control Division Medlife Healthcare Limited Dhaka Bangladesh

As a matter of fact, infectious diseases hamper everyone’s life and produce a lifelong threat to everyone neutral of age, sex, lifestyle and socio-economic status. Nowadays, come into the sight of Chikungunya viral (chikv) infection injured many Asian and African countries, also deliberated threat in rising countries and also low socio-economic countries. chikv is a positive-sense, enveloped single-stranded, RNA virus belonging to the genus Alphavirus, family Togaviridae. As the Dengue & Chikungunya viruses are spread simultaneously at the same time, so it is tough to identify them. In our resource-limited countries, swift detection of chikv by RT-LAMP is the simplest molecular technique in low-equipment settings without the use of any expensive decoration. Heat-treated centrifuged and uncentrifuged samples were used in this study and they showed the same result (100%). Different instruments like heat block, water bath, conventional thermal cycler & real-time thermal cycler were used to amplify the chikv RNA and they indicated that 100% samples were identified by all four instruments. The amplified products were visualized by turbidity test, color change by HNB, step-ladder band pattern in agarose gel electrophoresis and amplification curve in real-time thermal cycler naked eye, here the results also showed 100% samples were determined by all visualized methods. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a recent technique for amplifying RNA under limited temperature, with high tangibility, quickness and competency. To identify the chikv RNA Reverse transcription loop-mediated isothermal amplification was fabricated and validated, and the results were also compared with reverse transcription polymerase chain reaction (RT-PCR). The sensitivity was 95.71% and specificity was 100%, these results indicate that RT-LAMP is a feasible method for quick detection of chikv RNA.

关键词： chikv Chikungynea RT-LAMP Infectious Diseases Viral Infection chikv-RNA

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