检索结果-南通市图书馆

Discrimination of marine algal taxonomic groups based on fluorescence excitation emission matrix, parallel factor analysis and chemtax

引用

Acta Oceanologica Sinica 2014年第12期33卷 192-205页

作者： CHEN Xiaona SU Rongguo BAI Ying SHI Xiaoyong YANG Rujun College of Chemistry and Chemical Engineering Ocean University of China

An in vivo three-dimensional fluorescence method for the determination of algae community structure was developed by parallel factor analysis （PARAFAC） and chemtax. The PARAFAC model was applied to fluo-rescence excitation-emission matrix （EEM） of 60 algae species belonging to five divisions and 11 fluorescent components were identified according to the residual sum of squares and specificity of the composition profiles of fluorescent. By the 11 fluorescent components, the algae species at different growth stages were classified correctly at the division level using Bayesian discriminant analysis （BDA）. Then the reference fluo-rescent component ratio matrix was constructed for chemtax, and the EEM-PARAFAC-chemtax method was developed to differentiate algae taxonomic groups. The correct discrimination ratios （CDRs） when the fluorometric method was used for single-species samples were 100% at the division level, except for Bacil-lariophyta with a CDR of 95.6%. The CDRs for the mixtures were above 94.0% for the dominant algae species and above 87.0% for the subdominant algae species. However, the CDRs of the subdominant algae species were too low to be unreliable when the relative abundance estimated was less than 15.0%. The fluorometric method was tested using the samples from the Jiaozhou Bay and the mesocosm experiments in the Xiaomai Island Bay in August 2007. The discrimination results of the dominant algae groups agreed with microscopy cell counts, as well as the subdominant algae groups of which the estimated relative abundance was above 15.0%. This technique would be of great aid when low-cost and rapid analysis is needed for samples in a large batch. The fluorometric technique has the ability to correctly identify dominant species with proper abundance both in vivo and in situ.

关键词： chemtax fluorescence excitatiqn-emission matrices in vivo fluorescent components,parallel factor analysis phytoplankton

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The modification and optimizing of the chemtax running in the South China Sea

引用

Acta Oceanologica Sinica 2015年第2期34卷 124-131页

作者： WANG Lei HUANG Bangqin LIU Xin XIAO Wupeng Fujian Provincial Key Laboratory for Coastal Ecology and Environmental Studies/Key Laboratory of Coastal and Wetland Ecosystems of Ministry of Education College of the Environment and Ecology Xiamen University

In order to determine the phytoplankton community composition, the modification and optimizing of the CHEMical TAXonomy（chemtax） running was carried out through samples grouping, successive run and evaluate the results for HPLC-pigment samples in the South China Sea（SCS）. The vertical distribution of the ratio of pigment to total Chl a（TChl a） exhibited three different patterns, including increasing with depth pattern（e.g., But-Fuco）, decreasing with depth pattern（e.g., Zea） and increasing at deep chlorophyll maximum（DCM） pattern（e.g., Hex-Fuco）. The vertical profiles for Fuco/TChl a and Pras/TChl a was higher in coast than in the shelf and basin, and the Zea and Dv-Chl a expressed conversely. So the samples in the coastal stations must be separated for the cluster analysis group procedure in the SCS. Successive run was introduced into the chemtax calculation and the output results were evaluated by the convergence of pigment/TChl a ratios. Most of the ratios were well converged at the fifth running, except Zea/TChl a for Prochlorococcus and Chl b/TChl a for prasinophytes and so on. To evaluate the fifth running＇s results, haptophytes_8 and chlorophytes were two phytoplankton groups with much uncertainty. But the fifth estimated value was better than running once was supported by the regression evidence between the measured pigment concentration and calculation values. Synechococcus was another component with much mutability, and the chemtax＇s result should be compared to the flow cytometry＇s cell abundance.

关键词： phytoplankton chemtax pigment/Chl a ratio successive run South China Sea

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Phytoplankton pigment pattern in the subsurface chlorophyll maximum in the South Java coastal upwelling system, Indonesia

引用

Acta Oceanologica Sinica 2018年第12期37卷 97-106页

作者： GAO Chunlei FU Mingzhu SONG Hongjun WANG Lei WEI Qinsheng SUN Ping LIU Lin ZHANG Xuelei First Institute of Oceanography Ministry of Natural Resources Qingdao 266061 China Laboratory of Marine Ecology and Environmental Science Pilot National Laboratory for Marine Science and Technology (Qingdao) Qingdao 266237 China Key Laboratory of Science and Engineering for Marine Ecological Environment Ministry of Natural Resources Qingdao 266061 China Third Institute of Oceanography Ministry of Natural Resources Xiamen 361005 China

Upwelling occurs on the coast of Java between June and October, forced by local alongshore winds associated with the southeasterly monsoon. This causes variations in phytoplankton community composition in the upwelling zone compared with the surrounding offshore area. Based on pigments analysis with subsequent calculations of group contributions to total chlorophyll a(Chl a) using chemtax, we studied the distribution and composition of phytoplankton assemblages in the subsurface chlorophyll maximum along the south coast of Java and the influence of upwelling. Nineteen phytoplankton pigments were identified using high-performance liquid chromatography, and chemtax analysis associated these to ten major phytoplankton groups. The phytoplankton community in the coastal area influenced by upwelling was characterized by high Chl a and fucoxanthin concentrations, indicating the dominance of diatoms. In contrast, in the offshore area, the Chl a and fucoxanthin concentrations declined to very low levels and the community was dominated by haptophytes represented by 19′-Hexanoyloxyfucoxanthin. Accordingly, microphytoplankton was found to be the major size class in the coastal area influenced by upwelling, while nanophytoplankton was most abundant in the offshore area. Low concentrations of other accessory pigments indicated less contribution from dinoflagellates,prasinophytes, chlorophytes and cryptophytes. Photo-pigment indices revealed that photosynthetic carotenoids(PSCs) were the largest component of the pigment pool, exceeding the proportion of Chl a, with the average PSCTP up to 0.62. These distribution trends can mainly be explained by phytoplankton adaption strategies to upwelling and subsurface conditions by changing species composition and adjusting the pigment pool.

关键词： Java upwelling phytoplankton pigment HPLC subsurface chlorophyll maximum chemtax size structure

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Phytoplankton pigment patterns and community composition in the northern South China Sea during winter

引用

Chinese Journal of Oceanology and Limnology 2011年第2期29卷 233-245页

作者：翟红昌宁修仁唐学玺郝锵乐凤凤乔静 College of Marine Life Science Ocean University of China Second Institute of Oceanography SOA State Key Lab of Satellite Ocean Environment Dynamics

Phytoplankton pigment patterns and community composition were investigated in the northern South China Sea using high-performance liquid chromatography and the chemtax software from February 11 to 23, 2009. We recognized four different vertical distribution patterns of pigments: chlorophyll a (Chl a)-like type, divinyl chlorophyll a (DV Chl a) type, even distribution type, and surface type. The average value of ratios of accessory photo-protective pigments (APP) to accessory photo-synthetic pigments was 0.89±0.63 in the upper 50 m and 0.16±0.06 below 50 m depth. With increasing depth, APP decreased and photo-synthetically active radiation was attenuated. There was an obvious succession in the phytoplankton community from inshore to the open sea. Diatoms were dominant in the inshore region, while pelagophytes, Prochlorococcus, cyanobacteria and prymnesiophytes were dominant in the open sea. The vertical distribution of phytoplankton also differed greatly from inshore to the open sea. In the coastal and shelf region, diatoms were important components in the whole water column. Cyanobacteria also had a high abundance at the Subsurface Chlorophyll a Maxima (SCM) in the shelf region. In the slope and open sea, Prochlorococcus and cyanobacteria were important groups above the SCM, while pelagophytes dominated below the SCM.

关键词： phytoplankton pigment community composition northern South China Sea HPLC chemtax

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Phytoplankton composition and its ecological effect in subsurface cold pool of the northern Bering Sea in summer as revealed by HPLC derived pigment signatures

引用

Acta Oceanologica Sinica 2014年第6期33卷 103-111页

作者： ZHUANG Yanpei JIN Haiyan LI Hongliang CHEN Jianfang WANG Bin CHEN Fajin BAI Youcheng LU Yong TIAN Shichao Laboratory of Marine Ecosystem and Biogeochemistry Second Institute of OceanographyState Oceanic Administration State Key Laboratory of Satellite Ocean Environment Dynamics Second Institute of OceanographyState Oceanic Administration College of Ocean and Meteorology Guangdong Ocean University

chemtax analysis of high-performance liquid chromatography （HPLC） pigment was conducted to study phytoplankton community structure in the northern Bering Sea shelf, where a seasonal subsurface cold pool emerges. The results showed that fucoxanthin （Fuco） and chlorophyll a （Chl a） were the most abundant diagnostic pigments, with the integrated water column values ranging from 141 to 2160 μg/m2 and 477 to 5 535 μg/m2, respectively. Moreover, a diatom bloom was identified at Sta. BB06 with the standing stock of Fuco up to 9214 μg/m3. The results of chemtax suggested that the phytoplankton community in the northern Bering Sea shelf was dominated by diatoms and chrysophytes with an average relative contribu- tion to Chl a of 80% and 12%, respectively, followed by chlorophytes, dinoflagellates, and cryptophytes. Dia- toms were the absolutely dominant algae in the subsurface cold pool with a relative contribution exceeding 90%, while the contribution of chrysophytes was generally higher in oligotrophic upper water. Additionally, the presence of a cold pool would tend to favor accumulation of diatom biomass and a bloom that occurred beneath the halocline would be beneficial to organic matter sinks, which suggests that a large part of the phytoplankton biomass would settle to the seabed and support a rich benthic biomass.

关键词： pigment phytoplankton community structure chemtax northern Bering Sea shelf subsurface cold pool

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

面向浮游植物类群遥感的HY-1C/D卫星数据应用初探

引用

遥感学报 2023年第1期27卷 128-145页

作者：孙德勇陈宇航刘建强王胜强何宜军南京信息工程大学海洋科学学院南京210044 自然资源部空间海洋遥感与应用重点实验室北京100081 江苏省海洋环境监测工程技术研究中心南京210044 自然资源部国家卫星海洋应用中心北京100081

浮游植物是全球初级生产力的重要贡献者,浮游植物群落结构的变化影响着初级生产力,进而影响着海洋的物质循环与能量转换,因此具体量化分析浮游植物各群落结构的生物量对了解浮游植物群落结构变化,进而了解全球初级生产力极其重要。本文... 详细信息

浮游植物是全球初级生产力的重要贡献者,浮游植物群落结构的变化影响着初级生产力,进而影响着海洋的物质循环与能量转换,因此具体量化分析浮游植物各群落结构的生物量对了解浮游植物群落结构变化,进而了解全球初级生产力极其重要。本文基于2016年与2018年4个渤海航次的实测遥感反射率数据和实测HPLC(High Performance Liquid Chromatography)浮游植物色素数据,通过chemtax(CHEMical TAXonomy)方法将HPLC色素数据转化为相应藻种浓度数据,其中硅藻、隐藻、蓝藻与绿藻对总叶绿素a的占比较大。结合奇异值分解和多元线性回归方法,构建适用于中国近海硅藻、隐藻、蓝藻和绿藻浓度的反演模型;利用留一交叉验证法对模型进行验证,结果表明:隐藻、蓝藻和绿藻模型精度较高,决定系数R2均在0.70及以上,硅藻R2为0.44(p均小于0.001),硅藻、隐藻、蓝藻和绿藻浓度反演模型的中值误差ME各为44.81%、45.34%、51.20%和62.80%。随后,将模型应用于国产HY-1C/D卫星海洋水色扫描仪COCTS(China Ocean Color&Temperature Scanner)的遥感反射率日产品数据,获得渤海4个藻种浓度的空间分布特征,发现与实测浓度的空间分布特征分布一致。进一步分析COCTS与MODIS-Aqua、GOCI-Ⅱ的藻种浓度反演模型精度,发现基于COCTS波段的隐藻浓度反演模型精度高于基于MODIS-Aqua、GOCI-Ⅱ波段模型,硅藻、蓝藻和绿藻浓度反演模型精度和MODIS-Aqua相近且均高于GOCI-Ⅱ。在藻种浓度监测的示范性应用上,COCTS效果更好。综上所述,国产卫星HY-1C/D数据在藻种浓度监测方面具有强大的应用潜力。

关键词：藻种浓度 chemtax 奇异值分解 HY-1C/D 渤海

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

结合光吸收的近海水体硅藻浓度反演模型构建

引用

遥感学报 2023年第2期27卷 363-375页

作者：樊杰孙德勇王胜强张海龙环宇何宜军南京信息工程大学海洋科学学院南京210044 江苏省海洋动力遥感与声学重点实验室南京210044 江苏省海洋环境监测工程技术研究中心南京210044

浮游植物种群结构对了解海洋碳循环,生物多样性等研究具有重要意义。针对某种浮游植物种群生物量的研究,可以掌握浮游植物种群结构变化特征。本研究基于航次实测色素数据,利用chemtax方法获取近海水体硅藻浓度信息,分析实测硅藻浓度数... 详细信息

浮游植物种群结构对了解海洋碳循环,生物多样性等研究具有重要意义。针对某种浮游植物种群生物量的研究,可以掌握浮游植物种群结构变化特征。本研究基于航次实测色素数据,利用chemtax方法获取近海水体硅藻浓度信息,分析实测硅藻浓度数据的空间分布状态。对高斯模型中心波长和半波宽参数进行优化,结合高斯模型分解获取的高斯峰值,分析其与硅藻浓度之间的相关关系,并遴选相关系数较高的高斯峰值。利用不同数学函数,开展高斯峰值与硅藻浓度之间的关系模型构建研究,并结合精度指标(决定系数R^(2),中值误差ME)判定各数学函数模型的硅藻浓度反演精度,以提出最优的反演模型。研究结果表明:(1)实测硅藻浓度整体上呈现出近岸高,离岸低的空间分布特征,高值区域主要分布在渤海湾及长江口附近;(2)硅藻浓度反演模型精度较高,通过留一交叉验证法对模型进行验证,决定系数为0.79,中值误差为49%,p值小于0.001,该模型为后续开展卫星遥感反演硅藻浓度的工作奠定了基础。

关键词：遥感硅藻浓度高斯模型 chemtax 中国近海

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于三维荧光光谱-平行因子技术联用的湖泊浮游藻化学分类学研究

引用

环境科学 2014年第3期35卷 924-932页

作者：陈小娜韩秀荣苏荣国石晓勇中国海洋大学化学化工学院海洋化学理论与工程技术教育部重点实验室青岛266100

浮游藻荧光分析技术因其能够实现现场、快速、低成本测定而受到广泛研究和应用.本研究以浮游藻活体三维荧光光谱(EEM)为基础,利用平行因子(PARAFAC)和chemtax发展了浮游藻群落组成荧光分析技术.首先,将PARAFAC模型应用于23种浮游藻的EEM... 详细信息

浮游藻荧光分析技术因其能够实现现场、快速、低成本测定而受到广泛研究和应用.本研究以浮游藻活体三维荧光光谱(EEM)为基础,利用平行因子(PARAFAC)和chemtax发展了浮游藻群落组成荧光分析技术.首先,将PARAFAC模型应用于23种浮游藻的EEM,通过残差分析、荧光成分谱形分析等方法确定浮游藻EEM由12个荧光成分组成;然后,利用Bayesian判别分析表明浮游藻12个荧光成分的组成具有明显的门类特征性;最后,以获得的12个荧光成分构建浮游藻"荧光成分比值矩阵",结合chemtax建立浮游藻荧光识别分析技术.通过测试表明,该技术对531个单种藻样品的平均识别正确率是99.1%,其中,绿藻的识别正确率为97.5%,其余藻的识别正确率为100%.对于95个实验室混合样品,优势藻和次优势藻的平均识别正确率分别为98.5%和90.5%;测定的平均相对含量分别为69.7%和26.4%.结果表明,本研究所建立的EEM-PARAFAC-chemtax方法能够实现浮游藻群落组成的快速定性定量测定.

关键词：浮游藻群落组成三维荧光光谱平行因子活体 chemtax

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Changes in the Composition and Abundance of Phytoplankton in a Coastal Lagoon of Baja California,Mexico,during 2011

引用

Open Journal of Marine Science 2015年第2期5卷 169-181页

作者： María Fernanda Gracia-Escobar Roberto Millán-Núnez Enrique Valenzuela-Espinoza Adriana González-Silvera Eduardo Santamaría-del-Angel Facultad de Ciencias Marinas Universidad Autónoma de Baja CaliforniaEnsenadaMéxico Instituto de Investigaciones Oceanológicas Universidad Autónoma de Baja CaliforniaEnsenadaMéxico

A time series with weekly sampling was conducted from February 20, to December 16, 2011 at a station in the interior of the San Quintín Bay to estimate seasonal changes in the composition and abundance of phytoplankton. Water temperature was recorded and the upwelling index was calculated for the period. Phytoplankton abundance was estimated and phytoplankton were identified by using an inverted microscope and the chemtax program. There were 16 positive phytoplankton anomalies during the year, but only three were considered to be blooms. The blooms were dominated by diatoms and were recorded in the winter, spring and summer. Different genera composed 80% of the total phytoplankton abundance of each of the blooms. The first bloom consisted of diatoms of the genera Pseudo-nitzschia sp. (15.7%), Skeletonema sp. (14.6%), Eucampia sp. (7%), and Navicula sp. (7%);a haptophyte of the genus Imantonia sp. (13.1%);and dinoflagellates of the genus Prorocentrum sp. (4.6%). The second bloom consisted mainly of diatoms of the genera Guinardia sp. (30.6%), Pseudo-nitzschia sp. (21.5%), Skeletonema sp. (14.5%), Chaetoceros sp. (8.4%) and Eucampia sp. (5.3%). The third bloom consisted of Chaetoceros sp. (46.3%), Pseudo-nitzschia sp. (22.6%), Skeletonema sp. (7.29%), and Imantonia sp. (6%). Dinoflagellates were observed in the winter, summer and autumn, but they contributed less biomass than diatoms. Prorocentrum sp., Gymnodinium sp., and Ceratium sp. were among the dinoflagellates that were observed. The differences in abundance and composition of phytoplankton groups in the blooms in San Quintín Bay during 2011 were due primarily to seasonal changes in the physical and chemical factors of the seawater and to upwelling events.

关键词： Phytoplankton Bloom Pigments chemtax Coastal Lagoon

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of Freshwater Influx on Phytoplankton in the Mandovi Estuary (Goa, India) during Monsoon Season: Chemotaxonomy

引用

Journal of Water Resource and Protection 2013年第3期5卷 349-361页

作者： Sushma G. Parab S. G. Prabhu Matondkar Helga do R. Gomes Joaquim I. Goes Lamont Doherty Earth Observatory at Columbia University New York USA National Institute of Oceanography Dona Paula Goa India

The Mandovi estuary is a prominent water body that runs along the west coast ofIndia. It forms an estuarine network with the adjacent Zuari estuary, connected via the Cumbharjua canal. The physico-chemical conditions seen in the Mandovi estuary are influenced by two factors: the fresh water runoff during the monsoon season (June-September) and the tidal influx of coastal seawater during the summer (October to May) season. However, the effects of monsoon related changes on the phytoplankton of the Mandovi estuary are not yet fully understood. An attempt to understand the same has been made here by applying the process of daily sampling at a fixed station throughout the monsoon season. It was noticed that the onset of the monsoon is responsible for an increase in nitrate levels upto 26 μM from a (chl a) as high as 14 μg·L-1 during the same period. The phytoplankton population was observed through both chemotaxonomy and microscopy and was found to be composed mainly of diatoms. chemtax analysis further uncovers the presence of several other groups of phytoplankton, the presence of which is yet to be reported in many other tropical estuaries. It includes chrysophytes, cyanobacteria, prasinophytes, prymnesiophytes and chlorophytes. The appearance of phytoplankton groups at various stages of the monsoon was recorded, and this data is discussed in relation to environmental changes in the Mandovi estuary during the monsoon season.

关键词： Phytoplankton Pigment Analysis Monsoon Freshwater Runoff chemtax

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：