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Effect of Icariin on apoptosis and expression of Fas,Fas ligand,B cell lymphoma,and Bcl-2-associated X protein in cd4＋ t lymphocytes from patients with ankylosing spondylitis

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Journal of traditional Chinese Medicine 2017年第2期37卷 207-213页

作者： Wang Hailong Jiang Quan Feng Xinghua Division of Rheumatology Guang'anmenHospital China Academy of Chinese Medical Science Beijing 100053 China Basic Medical School Guiyang University of Chinese Medicine Guiyang 550025 China the SecondAffiliated Hospital Guiyang University of Chinese MedicineGuiyang 550003 China Division of Rheurnatology Guang'anrnen Hospital China Academy of Chinese MedicalScience Beijing 100053 China

OBJECtIVE:to investigate the effects of icariin on apoptosis and the expression of Fas, Fas ligand(Fas L), B cell lymphoma(Bcl-2), and Bcl-2-associated X protein(Bax) in cd4+ t lymphocytes from patients with ankylosing ***:Primary cultures of peripheral blood cd4+ t lymphocytes were established and treated with icariin at high, medium, and low doses(0.5,0.25, and 0.125 mg/mL).Sulfasalazine treated and helthy cells were used as *** of treated cells was determined by flow *** transcription polymerase chain reaction and enzyme-linked immunosorbent assays were used to determine the effects of icariin on the expression of Fas, Fas L, Bcl-2, and *** activity of caspase 8 and caspase 3 was determined by a colorimetric ***:the m RNA and protein expression of Fas,and activity of caspase 8 and caspase 3 in cd4+ t lymphocytes were increased by icariin(P the m RNA and protein expression of Bcl-2 was decreased(P the expression of Fas L and Bax were not significantly different between *** proapoptotic effects of icariin were ***:Icariin induces the apoptosis of cd4 + t cells from patients with AS comparing to normal ***, the induction of apoptosis may be the likely mechanism of action of icariin's antirheumatics activities.

关键词： Spondylitis ankylosing Fas Fas Ligand protein Lymphoma B-Cell Bcl-2-associated X protein cd4-positive t-lymphocytes Apoptosis Icariin

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Colon cancer and the immune system:the role of tumor invading t cells

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World Journal of Gastroenterology 2006年第45期12卷 7233-7238页

作者： Maximilian Waldner Carl C Schimanski Markus F Neurath I. Department of Medicine University of Mainz Mainz Germany

Colon cancer is still one of the leading causes of cancer death worldwide. Although the host immune system has been shown to react against tumor cells, mainly through tumor infi ltrating lymphocytes and NK cells, tumor cells may utilize different ways to escape anti-tumor immune response. tumor infi ltration of cd8+ and cd4+ (t-bet+) effector t cells has been attributed to a beneficial outcome, and the enhancement of t cell activation through t cell receptor stimulation and co-stimulatory signals provides promising strategies for immunotherapy of colon cancer. Growing evidence supports a role for the Fas/FasL system in tumor immunology, although the mechanisms and consequences of FasL activation in colon cancer are not completely understood. In animal models, depletion of regulatory t cells (cd4+ cd25+ t cells) can enhance the anti-tumor immune response under certain conditions. taken together, recent insights in the immune reaction against colon carcinoma have provided new approaches to immunotherapy, although much remains to be learned about the exact mechanisms.

关键词： cd4-positive t-lymphocytes cd8-positive t-lymphocytes Immunology Colonic neoplasms therapy Colorectal neoplasms Humans lymphocytes tumor-infiltrating tumor escape

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Study on Cytokines IL-2,IL-6,IL-10 in Patients of Chronic Allergic Rhinitis treated with Acupuncture

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Journal of traditional Chinese Medicine 2002年第2期22卷 104-111页

作者： FILOMENA BANGRAZI PEttI M.Sc.Ph.D. ALDO LIGUORI M.D. FLORA IPPOLItI M.D.Ph.D. Paracelso Chair

OBJECtIVES: to observe the plasmatic concentration of IL-6, IL-10 and IL-2 in the patient of chronic allergic rhinitis before and after acupuncture therapy. MEtHODS: Cytokine levels were determined before and after treatment in 30 healthy volunteers (Group A) and 90 patients of chronic allergic rhinitis (Group B) with an increased plasma IL-10 level. Group B was then divided into 3 subgroups: 30 patients treated with real acupuncture (Group B1); 30 patients treated with sham acupuncture (Group B2); 30 non-treated patients (Group B3). RESULtS: the allergic subjects of group B1, compared with controls, showed a significant reduction of IL-10 after a specific treatment with acupuncture (P

关键词： Acupuncture therapy Adult cd4-positive t-lymphocytes Female Humans Interleukin-10 Interleukin-2 Interleukin-6 Male Middle Aged Rhinitis, Allergic, Perennial

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Expression of chemokine receptors CCR3, CCR5 and CXCR3 on cd4^＋ t cells in CBA/J×DBA/2 mouse model, selectively induced by IL-4 and IL-10, regulates the embryo resorption rate

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Chinese Medical Journal 2009年第16期122卷 1917-1921页

作者： JIANG Pei-juan ZHAO Ai-min BAO Shi-min XIAO Shi-jin XIONG Miao Department of Obstetrics and Gynecology Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200127 China Laboratory Animal Center of Shanghai Chinese Academy of Science Shanghai 201615 China Department of Obstetrics and Gynecology Shanghai Pudong New Area Gongli Hospital Shanghai 200135 China

Background Chemokines and their receptors have been a research focus in transplantation immunology. Chemokines and their receptors play a role in lymphocyte recruitment and differentiation process. this study aimed to observe whether IL-4 and IL-10 may regulate the expression of chemokine receptors CCR3, CCR5 and CXCR3 on cd4^＋ t cells in CBA/J×DBA/2 mouse model and to explore the role of CCR3, CCR5, CXCR3 in immune tolerance in pregnancy. Methods the mouse model of spontaneous abortion （CBA/J×DBA/2） and the normal pregnant mouse model （CBA/J×BALB/c） were used. CBA/J×DBA/2 mice were injected with IL-4 （CBA/J×DBA/2-IL-4）, IL-4 and IL-10 （CBA/J×DBA/2-IL-4＋IL-10）, or normal saline （CBA/J×DBA/2-NS） as a control. the expression of CCR3, CCR5 and CXCR3 on cd4^＋ t cells from mouse peripheral blood was measured by the double-labelled FCM method, and the embryo resorption rate was also examined. Results the embryo resorption rate in the CBA/J×DBA/2 group without any treatment was significantly higher than that in the CBA/J×BALB/c group （17.9% vs 3.7%, P 〈0.01）. the embryo resorption rate in the CBA/J×DBA/2 group immunized with IL-4 or IL-4 together with IL-10 was significantly decreased, compared with that in the control and NS groups respectively. CCR3 expression on cd4^＋ t cells in the CBA/J×DBA/2 group without any treatment was significantly lower than that in the CBA/J×BALB/c group （0.3738±0.3575 vs 1.2190±0.2772, P 〈0.01）; both CCR5 （3.0900±1.5603 vs 1.2390±0.6361, P〈0.01） and CXCR3 （2.4715±0.9074 vs 0.9200±0.5585, P 〈0.01） expressions on cd4^＋ t cells of the CBA/J×DBA/2 group without any treatment were significantly higher than those of the CBA/J×BALB/c group. Significant up-regulation of CCR3 and down-regulation of CXCR3 were found in the CBA/J×DBA/2 group treated with IL-4 （CCR3： 2.0360±0.6944, CXCR3： 1.3510±0.5263, P〈0.01） or IL-4 and IL-10 （CCR3： 1.8160±1.0947, CXCR3：1.0940±0.7168, P〈0.01）. Because of the CCR5, IL-4 and IL-10 （1.9400±0.8504 vs 3.0900±1.5603, P 〈0.0

关键词： chemokine receptors cd4-positive t-lymphocytes abortion, spontaneous interleukin-4 interleukin-10

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Relationship between expression of chemokine receptors CCR3, CCR5 and CXCR3 on cd4^＋ t cells and spontaneous abortion in mice

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Chinese Medical Journal 2009年第4期122卷 390-395页

作者： JIANG Pei-juan LIN Qi-de BAO Shi-min ZHAO Ai-min ZHANG Yu XIAO Shi-jin Department of Obstetrics and Gynecology Renji Hospital Schoolof Medicine Shanghai Jiao Tong University Shanghai 200127China Laboratory Animal Center of Shanghai Chinese Academy ofScience Shanghai 201615 China

Background Previous studies have shown that local immune cells in the feto-maternal interface are recruited from peripheral blood, and that chemokines and their receptors play an initial and key role in this recruitment process. In this study, we aimed to determine whether spontaneous abortion is associated with the expression of chemokine receptors CCR3, CCR5, and CXCR3 on cd4^＋ t cells. Methods Peripheral blood, spleen, and thymus were collected from the spontaneous abortion mouse model CBA/JxDBA/2 （SA group, n=14）, the normal pregnant mouse model CBA/JxBALB/c （NP group, n=13）, and normal non-pregnant CBA/J mice （NNP group, n=11）. the number of chemokine receptors CCR3, CCR5, and CXCR3 expressed on cd4^＋ t cells was measured by double-label flow cytometry （FCM） method. Results In peripheral blood, the SA group had significantly lower CCR3 expression （P 〈0.01） and higher CCR5 and CXCR3 expression （P 〈0.01） on cd4^＋ t cells than did the NP group. But comparing these chemokines between the SA and NNP groups, there was no significant difference （P 〉0.05）. In spleen, the SA group expressed significantly lower CCR3 expression （P 〈0.01） and higher CCR5 and CXCR3 expression （P 〈0.05） on cd4^＋ t cells than did the NP group. When compared with the NNP group, the SA group had significantly higher CCR3 expression （P 〈0.01）, but was not statistically different with regards to the other two chemokines （P 〉0.05）. In thymus, the SA group had significantly lower CCR3 expression （P 〈0.05） and higher CXCR3 expression （P 〈0.05） on cd4^＋ t cells than the NP group, with no significant difference in CCR5 expression （P 〉0.05）. Compared with the NNP group, the SA group had higher CCR3 expression （P 〈0.01）, but there was no statistical difference in CXCR3 and CCR5 expression （P 〉0.05） between the two groups. Conclusion the abnormal expression of CCR3, CCR5 and CXCR3 on cd4^＋ t cells may play an important role in the pathogenesis of spontaneous abortion.

关键词： receptors, CCR3, CCR5, CXCR3 cd4-positive t-lymphocytes abortion, spontaneous

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t helper cells in patients with chronic hepatitis B virus infection

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Chinese Medical Journal 2002年第3期115卷 422-424页

作者：姜荣龙冯筱榕郭亚兵卢桥生侯金林骆抗先富宁第一军医大学南方医院感染内科广州510515

OBJECtIVE: to investigate the compositions of th1/th2/th3 cells in chronic hepatitis B virus (HBV)-infected individuals by determining the expression of interleukin-4 (IL-4), inetrferon-gamma (IFN-gamma), and transform growth factor-beta (tGF-beta) in single cd4(+) t cells isolated from peripheral blood mononuclear cells (PBMCs) and the role of polarized th cell populations in chronic HBV-infection was discussed. MEtHODS: PBMCs from chronically infected HBV individuals were isolated, stimulated by PMA/Ionomycin/Monensin, and IL-4, IFN-gamma and tGF-beta production by cd4(+) t cells was determined by using fluorescence activated cell sorter (FACS) analysis. RESULtS: the percentage of IFN-gamma-producing t cells, IL-4-producing t cells and tGF-beta-producing t cells ranged from 2.3% - 18.6%, 1.1% - 8.7% and 0.7% - 7.1% respectively in cd4(+) t cells from non-infected individuals. Most of cd4(+) t cells from PBMCs in chronically infected HBV individuals were th0 cells. the proportion of th1 cells increased significantly with hepatic inflammatory activity, and in the active period of chronic hepatitis B infection were higher than those in the non-active period (P 0.05), but were higher than that from controls (P

关键词： cd4-positive t-lymphocytes Hepatitis B, Chronic Humans Interferon type II Interleukin-4 t-lymphocytes, Helper-Inducer th1 Cells th2 Cells transforming Growth Factor beta

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