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Type III interferon-induced cbfβinhibits HBV replication by hijacking HBx

Cellular & Molecular Immunology 2019年第4期16卷 357-366页

作者： Fengchao Xu Hongxiao Song Qingfei Xiao Na Li Hong Zhang Genhong Cheng Guangyun Tan Department of Immunology Institute of Translational MedicineThe First Hospital of Jilin UniversityChangchunJilin 130061China Department of Nephrology The First HospitalJilin UniversityChangchunJilin 130021China Department of Obstetrics The First Hospital of Jilin UniversityChangchunJilin 130021China Phase I Clinical Research Center The First Hospital of Jilin UniversityJilin 130021China Department of Microbiology Immunology and Molecular GeneticsUniversity of CaliforniaLos AngelesCA 90095USA Center of Systems Medicine Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005 Suzhou Institute of Systems Medicine SuzhouJiangsu 215123China

Hepatitis B virus(HBV)and its associated chronic infection remain serious health threats ***,there is still no impactful approach for clinical treatment of hepatitis B ***,developing a better understanding of the interactions between HBV and its host is particularly *** infection has been reported to induce type-III but not type-I or type-II interferon(IFN).In this study,we identified cbfβ,an HIV enhancer,as an HBV restriction factor that is specifically induced by type-III IFN in the early stages of HBV ***-III IFN-induced IL-10 played an important role in the production of cbfβ.Interestingly,the interaction between cbfβ-and HBV-encoded regulatory protein X(HBx)enhanced the stability of cbfβ,but notably blocked HBx-mediated promotion of HBV ***βexpression was lower in HBV patients than in healthy persons,and the addition of serum from HBV patients inhibited cbfβexpression in HepG2 *** the contrary,HBV via HBsAg inhibited type-III IFN-induced cbfβexpression and decreased the anti-HBV activity of type-III IFN,suggesting that HBV inhibits antiviral interferon-stimulated gene(ISG)expression and induces IFN ***,our results demonstrate that type-III IFN-triggered and IL-10-induced cbfβare crucial factors for inhibiting HBV replication,and the HBx–cbfβ–HBsAg axis reveals a new molecular mechanism of interaction between HBV and its hosts.

关键词： inhibited interferon cbf

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An apple rootstock overexpressing a peach cbf gene alters growth and flowering in the scion but does not impact cold hardiness or dormancy

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Horticulture Research 2016年第1期3卷 287-295页

作者： Timothy S Artlip Michael E Wisniewski Rajeev Arora John L Norelli USDA-ARS Appalachian Fruit Research StationKearneysvilleWVUSA Department of Horticulture Iowa State UniversityAmesIAUSA

The C-repeat binding factor(cbf)transcription factor is involved in responses to low temperature and water deficit in many plant *** of cbf genes leads to enhanced freezing tolerance and growth inhibition in many *** overexpression of a peach cbf(Ppcbf1)gene in a transgenic line of own-rooted apple(Malus×domestica)M.26 rootstock(T166)trees was previously reported to have additional effects on the onset of dormancy and time of spring *** the current study,the commercial apple cultivar‘Royal Gala’(RG)was grafted onto either non-transgenic M.26 rootstocks(RG/M.26)or transgenic M.26(T166)rootstocks(RG/T166)and field grown for 3 *** Ppcbf1 transcript was detected in the phloem or cambium of RG scions grafted on T166 rootstocks indicating that no graft transmission of transgene mRNA had *** contrast to own-rooted T166 trees,no impact of Ppcbf1 overexpression in T166 rootstocks was observed on the onset of dormancy,budbreak or non-acclimated leaf-cold hardiness in RG/T166 ***,however,as measured by stem caliper,current-year shoot extension and overall height,was reduced in RG/T166 trees compared with RG/M.26 *** flowering was evident in both RG/T166 and RG/M.26 trees in the second season,the number of trees in flower,the number of shoots bearing flowers,and the number of flower clusters per shoot was significantly higher in RG/M.26 trees than RG/T166 trees in both the second and third year after *** levels of RGL(DELLA)gene expression were observed in RG/T166 trees and T166 trees,which may play a role in the reduced growth observed in these tree types.A model is presented indicating how cbf overexpression in a rootstock might influence juvenility and flower abundance in a grafted scion.

关键词： impact cbf grafted

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The transcription factor ICE1 functions in cold stress response by binding to the promoters of cbf and COR genes

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Journal of Integrative Plant Biology 2020年第3期62卷 258-263页

作者： Kai Tang Lun Zhao Yuying Ren Shuhua Yang Jian-Kang Zhu Chunzhao Zhao Shanghai Center for Plant Stress Biology and CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of SciencesShanghai 200032China Department of Horticulture and Landscape Architecture Purdue UniversityWest LafayetteIN 47907USA University of the Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan UniversityKaifeng 475004China

A recent paper by Kidokoro et al.(2020)in The Plant Cell reported a transgene-dependent transcriptional silencing phenomenon in the dominant ice1-1 Arabidopsis mutant containing the cbf3-LUC reporter,and questioned whether ICE1 may regulate cbf genes and may be involved in plant cold ***,we evaluate available evidence supporting the involvement of ICE1 in plant cold response,and provide ChIP-seq data showing ICE1 binding to the promoters of cbf genes and other regulatory genes known to be critical for cold response as well as to the promoters of some COR genes.

关键词： ICE1 cbf stress

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Vitis amuerensis cbf3 Gene Isolation,Sequence Analysis and Expression

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Agricultural Sciences in China 2010年第8期9卷 1127-1132页

作者： WANG Zhan-bin FENG Lian-rong WANG Jin-jie WANG Zhi-ying Northeast Forestry University/Key Labotatory of Forest Tree Genetic Improvement and Biotechnology Ministry of Agricuture Harbin 150040 P.R.China Institute of Poplar of Liaoning Province Gaizhou 115200 P.R. China

The full length cDNA sequence of cbf3 （CRT/DRE-binding factor） was cloned from Vitis amurensis by reverse transcription polymerase chain reaction （RT-PCR） using the primers designed based on cbf genes available in GenBank. Sequence analysis showed that the gene had 854 bp long and its coding sequence contained 720 bp, encoding a protein with 239 amino acids and an AP2 structural domain. The molecular mass of cbf3 was predicted to be 25.9 kDa and its theoretical isoelectric point was 7.02 and aliphatic index was 59.29. The average hydropathicity of the protein was -0.551. The tertiary structures of cbf3 were also analyzed. The prokaryotic expression vector pGEX-4T-cbf3 containing cbf3 gene was constructed and cbf3 fusion protein （52 kDa） was produced in Escherichia coli after induction with 1 mmol L-1 IPTG. Further studies are needed to evaluate its potential application for improving plant resistance to cold and other stress condition such as drought and salinity.

关键词： Vitis amurensis cbf gene cloning sequence analysis expression in prokaryote

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The precise regulation of different COR genes by individual cbf transcription factors in Arabidopsis thaliana

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Journal of Integrative Plant Biology 2017年第2期59卷 118-133页

作者： Yihao Shi Jiaying Huang Tianshu Sun Xuefei Wang Chenqi Zhu Yuxi Ai Hongya Gu State Key Laboratory for Protein and Plant Gene Research College of Life Sciences Peking University Beijing 100871 China

The transcription factors cbf1/2/3 are reported to play a dominant role in the cold responsive network of Arabidopsis by directly regulating the expression levels of cold responsive （COR） genes. In this study, we obtained CRISPR/Casg-mediated loss-of-function mutants of cbf1-3. Over 3,ooo COR genes identified by RNA-seq analysis showed a slight but significant change in their expression levels in the mutants compared to the wild- type plants after being treated at 4 ℃ for 12 h. The C-repeat （CRT） motif （5＇-CCGAC-3＇） was enriched in promoters of genes that were up-regulated by cbf2 and cbf3 but not in promoters of genes up-regulated by cbfI. These data suggest that cbf2 and cbf3 play a more important role in directing the cold response by regulating different sets of downstream COR genes. More than 2/3 of COR genes were co-regulated by two or three cbfs and were involved mainly in cellular signal transduction and metabolic processes; less than 1[3 of the genes were regulated by one cbf, and those genes up-regulated were enriched in cold-related abiotic stress responses. Our results indicate that cbfs play an important role in the trade-off between cold tolerance and plant growth through the precise regulation of COR genes in the complicated transcriptional network.

关键词： CRISPR Cas9 cbf cold tolerance COR single cbf-regulated genes

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Nitric Oxide Regulates Mitochondrial Fatty Acids and Promotes cbf Expression of Peach Fruit during Cold Storage

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Phyton-International Journal of Experimental Botany 2022年第2期91卷 409-427页

作者： Xiaoshan Guo Dandan Huang Siyu Wen Yang Bai Shuhua Zhu Jianrong Feng Department of Horticulture College of AgricultureShihezi UniversityShihezi832000China College of Chemistry and Material Science Shandong Agricultural UniversityTaian271018China

Low-temperature storage is convenient for postharvest preservation of peach fruit,but peach fruit is sensitive to cold damage,which lowers its *** oxide(NO)has the potential to improve the bitter resistance of peach *** this work,peach fruit was treated with 15μmol L^(−1)NO and 5μmol L^(−1)c-PTIO[2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxo-3-oxide],to study changes in mitochondrial fatty acids and expression of the C-repeat binding factor(cbf).The results showed that 15μmol L^(−1)exogenous NO significantly maintained fruit quality,reduced peroxidation of mitochondrial fatty acids,increased the activities of the antioxidants superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),ascorbic acid peroxidase(APX),and reduced the content of hydrogen peroxide(H_(2)O_(2)).Meanwhile,NO treatment suppressed the increase in browning index and ion leakage rate,increased the activity of S-nitrosoglutathione reductase(GSNOR),the contents of S-nitrosothiols(SNOs),and the ratios of mitochondrial NAD^(+)/NADH and NADP^(+)/NADPH,increased the expression levels of Ppcbf1/5/***,the expression levels of Ppcbf2/3/4 were not significantly regulated by exogenous *** treated with c-PTIO showed opposite effects to those treated with exogenous *** results suggest that exogenous NO can improve antioxidant capacity,preserve mitochondrial fatty acids,and upregulate the expression of Ppcbf1/5/6 to alleviate cold tolerance and maintain the peach quality during storage.

关键词： Peach cold storage nitric oxide mitochondrial fatty acid cbf

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Identification and characterization of cbf/DREB1-related genes in Populus hopeiensis

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Forestry Studies in China 2008年第3期10卷 143-148页

作者： WANG Ze-liang AN Xin-min LI Bo REN Yuan-yuan JIANG Xi-bing BO Wen-hao ZHANG Zhi-yi Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education Beijing Forestry University Beijing 100083 P. R. China

The dehydration-responsive element-binding factor （DREB） is a plant-specific family of transcription factors and plays an important role in plant response and adaptation to abiotic stress. In the present work, two highly similar cbf/DREB l-like genes, designated as Phcbf4a and Phcbf4b, were identified from Populus hopeiensis. These two genes contain all conserved domains known to exist in other cbf/DREB1 genes. In the AP2 domain, there is only one different amino acid residue between Phcbf4a and Phcbf4b, alanine or valine, a nonpolar amino acid, suggesting that Phcbf4a and Phcbf4b may have similar DNA binding ability. Their expression is induced by water-loss treatment and their expression patterns are similar. Moreover, with a genomic DNA as template, the presence of the same bands in PCR products as those in expression pattern analysis indicated that Phcbf4a and Phcbf4b exist in the genome ofP. hopeiensis. Their detailed functions are discussed and will need further study.

关键词： cbf DREB abiotic stress Populus hopeiensis

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BZR1 Positively Regulates Freezing Tolerance via cbf-Dependent and cbf-Independent Pathways in Arabidopsis

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Molecular Plant 2017年第4期10卷 545-559页

作者： Hui Li Keyi Ye Yiting Shi Jinkui Cheng Xiaoyan Zhang Shuhua Yang State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beijing 100193 China These authors contributed equally to this article.

Cold stress is a major environmental factor that adversely affects plant growth and development. The C-repeat binding factor/DRE binding factor 1 （cbf/DREB1） transcriptional regulatory cascade has been shown to play important roles in plant response to cold. Here we demonstrate that two key components of brassinosteroid （BR） signaling modulate freezing tolerance of Arabidopsis plants. The loss-of-function mutant of the GSK3-1ike kinases involved in BR signaling, bin2-3 bill bil2, showed increased freezing tolerance, whereas overexpression of BIN2 resulted in hypersensitivity to freezing stress under both non-acclimated and acclimated conditions. By contrast, gain-of-function mutants of the transcription factors BZR1 and BES1 displayed enhanced freezing tolerance, and consistently cold treatment could induce the accumulation of dephosphorylated BZR1. Biochemical and genetic analyses showed that BZR1 acts upstream of cbf1 and cbf2 to directly regulate their expression. Moreover, we found that BZR1 also regulated other COR genes uncoupled with cbfs, such as WKRY6, PYL6, SOCl, JMT, and SAG21, to modulate plant response to cold stress. Consistently, wrky6 mutants showed decreased freezing tolerance. Taken together, our results indicate that BZR1 positively modulates plant freezing tolerance through cbf-dependent and cbf-independent pathways.

关键词： BIN2 BZR1 freezing tolerance cbf Arabidopsis

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外源水杨酸对低温下杏花抗氧化酶和cbf转录因子表达的影响?

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植物生理学报 2014年第2期50卷 171-177页

作者：张俊环王玉柱孙浩元杨丽姜凤超北京市农林科学院林业果树研究所北京100093

为探讨水杨酸(SA)对杏花抗寒性的影响机制,以早熟品种‘骆驼黄’杏的显蕾期花枝为试材,分析–2℃的低温下适宜浓度SA及其抑制剂ABT和PAC对杏花MDA、抗氧化酶和cbf转录因子的影响。结果表明,–2℃低温条件下,对照和2个SA抑制剂处理的杏... 详细信息

为探讨水杨酸(SA)对杏花抗寒性的影响机制,以早熟品种‘骆驼黄’杏的显蕾期花枝为试材,分析–2℃的低温下适宜浓度SA及其抑制剂ABT和PAC对杏花MDA、抗氧化酶和cbf转录因子的影响。结果表明,–2℃低温条件下,对照和2个SA抑制剂处理的杏花细胞膜系统均受到严重伤害,CAT、POD和SOD等抗氧化酶活性降低,MDA含量明显升高。而SA预处理的杏花在低温胁迫期间抗氧化酶活性增强,MDA含量比对照和抑制剂处理的有明显降低且相对稳定。通过荧光定量检测cbf转录因子的表达水平,表明SA能诱导杏花cbf基因的表达,尤其在低温处理3 h时,SA预处理的杏花中cbf的表达量明显高于对照和SA抑制剂处理。由此认为,适宜浓度的外源SA可能是通过调控低温下杏花中cbf转录因子的表达、增强细胞的抗氧化酶活性,减轻低温造成的膜脂过氧化伤害,从而在一定程度上增强了杏花的抗寒性。

关键词：杏花抗氧化酶 cbf 低温水杨酸

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cbf转录因子在植物抗逆和生长发育中的重要功能

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植物生理学报 2017年第12期53卷 2045-2056页

作者：李健王雅晴刘洋杨兴洪山东农业大学生命科学学院作物生物学国家重点实验室山东泰安271018

cbf/DREB转录因子属于AP2(APETALA2)转录因子家族,能够识别下游COR(cold-regulated)基因启动子上的CRT/DRE顺式作用元件。在低温等非生物胁迫条件下,cbf(C-repeat binding factor)转录因子通过结合在CRT/DRE元件上激活COR基因的表达,从而提高植物抗逆能力。另外,cbf转录因子也会影响植物的生长和发育。本文从cbf转录因子的结构、调控cbf的上游调节因子、在应对非生物胁迫过程中激素信号与cbf通路的关系、cbf在植物适应逆境胁迫中的作用、不同cbf之间作用的共性与特异性,以及cbf对植物生长发育的影响等方面,阐述了cbf转录因子的调控机制及其在植物适应逆境胁迫和生长发育等方面的重要作用。

关键词： cbf DREB 非生物胁迫抗逆性生长发育

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