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Microprofiles of activated sludge aggregates using microelectrodes in completely autotrophic nitrogen removal over nitrite （canon） reactor

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Frontiers of Environmental Science & Engineering 2016年第2期10卷 390-398页

作者： Yongtao LV Xuan CHEN Lei WANG Kai JU Xiaoqiang CHEN Rui MIAO Xudong WANG School of Environmental and Municipal Engineering Xi'an University of Architecture and Technology Xi'an 710055 China College of Environment and Resource Shaanxi University of Science and Technology Xi'an 710021 China

Microsensor measurements and fluorescence in situ hybridization （FISH） analysis were combined to investigate the microbial populations and activities in a laboratory-scale sequencing batch reactor （SBR） for completely autotrophic nitrogen removal over nitrite （canon）. Fed with synthetic wastewater rich in ammo- nia, the SBR removed 82.54-5.4% ofinfluent nitrogen and a maximum nitrogen-removal rate of 0.52kgN.m 3.d-l was achieved. The FISH analysis revealed that aerobic ammonium-oxidizing bacteria （AerAOB） Nitrosomonas and anaerobic ammonium-oxidizing bacteria （AnAOB） dominated the community. To quantify the microbial activities inside the sludge aggregates, microprofiles were measured using pH, dissolved oxygen （DO）, NH＋, NO＋ and NO3 microelectrodes. In the outer layer of sludge aggregates （0-700 μm）, nitrite-oxidizing bacteria （NOB） showed high activity with 4.1 gmol-cm3＋ h-1 of maximum nitrate production rate under the condition of DO concentration higher than 3.3 mg. L 1. Maximum AerAOB activity was detected in the middle layer （depths around 1700 μm） where DO concentration was 1.1 μg. L-1. In the inner layer （2200-3500μm）, where DO concentration was below 0.9 mg. L-1, AnAOB activity was detected. We thus showed that information obtained from microscopic views can be helpful in optimizing the SBR performance.

关键词： aerobic ammonium-oxidizing bacteria anae-robic ammonium-oxidizing bacteria nitrite-oxidizing bac-teria microelectrodes canon

Characterization of canon reactor performance and microbial community shifts with elevated COD/N ratios under a continuous aeration mode

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Frontiers of Environmental Science & Engineering 2019年第1期13卷 73-85页

作者： Yao Zhang Yayi Wang Yuan Yan Haicheng Han Min Wu State Key Laboratory of Pollution Control and Resources Reuse Shanghai Institute of Pollution Control and Ecological Security College of Environmental Science and Engineering Tongji University Shanghai 200092 China

The effects of increasing COD/N on nitrogen removal performance and microbial structure were investigated in a SBR adopting a completely autotrophic nitrogen removal over nitrite process with a continuous aeration mode (DO at approximately 0.15-0.2 mg/L). As the COD/N increased from 0.1 to W0.59, the nitrogen removal efficiency QMRE) increased from 88.7% to 95.5%;while at COD/N ratios of 0.59-0.82, the NRE remained at 90.7%-95.5%. As the COD/N increased from 0.82 to 1.07, the NRE decreased continuously until reaching 60.1%. Nitrosomonas sp.(AOB) and Candidatus Jet tenia (anammox bacteria) were the main functional genera in the SBR. As the COD/N increased from 0.10 to 1.07, the relative abundance of Nitrosomonas decreased from 13.4% to 2.0%, while that of Candidatus Jettenia decreased from 35% to 9.9% with COD/N < 0.82 then increased to 45.4% at a COD/N of 1.07. Aerobic heterotrophic bacteria outcompeted AOB at high COD loadings (650 mg/L) because of oxygen competition, which ultimately led to deteriorated nitrogen removal pertormance.

关键词： canon process COD/N ratio Anammox Ammonia oxidizing bacteria Aerobic heterotrophic bacteria

Drivers of Forecasting the Behavioral Intention and Acceptance Behavior of the Hail canon Technology(HCT): Using Logistic and System Dynamics Modeling

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Chinese Geographical Science 2023年第3期33卷 549-564页

作者： ABADI Bijan HAGHANINIA Mohammad Faculty of Agriculture University of MaraghehGolshahr Town 55181-83111Iran

Globally known about the drivers through which farmers are instigated to uphold and use Hail canon Technology(HCT)is ***,this article intended to examine the drivers of forecasting the behavioral intention and acceptance behavior of the HCT,249 apple farmers from northwestern Iran were recruited,including adopters(n1=114)and non-adopters(n2=135).The conceptual foundation included demographic theory,resource-based theory,theory of planned behavior,innovation diffusion model,and institutional support *** also used the system dynamics model(SDM)in the Netlogo to assess the results of the conventional statistical approach(i.e.,the logistic model).Authenticated the fitness of conceptual model with the data,logistic model manifests that the most outstanding determinants of the acceptance of HCT entail age,experience,total land size,income,attitude,compatibility,visibility,relative advantage,and financial *** the SDM,it was also shown that the results of the logistic model are confirmed by the *** conclusion,management implications are available for the university extension to eliminate the adoption obstacles and stir up farmers to join in applying HCT,furthermore,researchers would avail themselves of remarks for future research.

关键词： canon visibility furthermore

canon Process for Nitrogen Removal from Effluents of Municipal Sewage Treatment Plants

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Transactions of Tianjin University 2013年第4期19卷 255-259页

作者：彭新红吴立波于宏兵艾丽娜付丽霞 College of Environmental Science and Engineering Nankai University

The feasibility and performance of nitrogen removal from municipal sewage were investigated through the completely autotrophic nitrogen removal over nitrite (canon) process in a continuous reactor. canon process was successfully started up with the transformation of nitrogen into gas by mass-balance analysis. For the synthetic waste-water (up to 480 mg NH4+-N/(L·d)), removal rates of the ammonia nitrogen and total nitrogen (TN) were about 80% and 55%, respectively, at 1.25 h hydraulic retention time (HRT). For the secondary effluent of municipal sewage, the effluent concentrations of NH4+-N and TN were below 5 mg/L and 9 mg/L, respectively. It is in accordance with the water quality standard for scenic environment with the reuse of urban recycling water (GB/T 18921-2002).

关键词： biodegradation canon optimisation municipal sewage

Galen’s place in Avicenna’s The canon of Medicine: Respect,confirmation and criticism

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Journal of Integrative Medicine 2020年第1期18卷 21-25页

作者： Sajjad Sadeghi Farzaneh Ghaffari Ghazaleh Heydarirad Mehdi Alizadeh Department of Traditional Medicine School of Traditional MedicineShahid Beheshti University of Medical SciencesTehran ***Iran

Galen(129–199 A.D.), an ancient Greek scholar, is one of the most influential and recognized physicians and surgeons in the history of medicine. He is one of the most influential Greek scholar in Persian medicine, who has been repeatedly cited by Iranian scholars. The purpose of this article is to describe the exact position of Galen in Avicenna’s The canon of Medicine, the most important work of Persian medicine. In The canon of Medicine, Galen is cited about 300 times, and Avicenna always admired and praised Galen repeatedly. Galen’s ideas were important to Avicenna, but there are also many elements of Galen’s views that Avicenna challenged or criticized. For example, Avicenna diverged from Galen’s philosophical view in medicine and rejected some of Galen’s pharmaceutical opinions and Galen’s views on the physiology of pain, the physiology of pulse, the same nature of the tendons and nerves and separation of the mind and body. Although the views of Greek scholars, especially Galen, in The canon of Medicine have been quoted abundantly, Avicenna revised the knowledge of the ancient scholars through critical thinking and relying on observation and testing, systematized the science of medicine and introduced many ideas and innovations. As such, Europeans considered Avicenna as the most prominent physician of the Islamic Golden Age.

关键词： Galen Avicenna The canon of Medicine Persian medicine

canon工艺短程硝化恢复调控及微生物种群结构变化

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中国环境科学 2019年第6期39卷 2354-2360页

作者：张铃敏常青龙史勤赵宇豪谢弘超王亚宜同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室上海污染控制与生态安全研究院

以短程硝化恶化的全程自养脱氮工艺(canon)污泥为研究对象,在间歇曝气序批式反应器(SBR)中开展自养脱氮运行效果的恢复调控研究,并基于微生物群落结构变化探究微生物调控机理.结果表明,高氨氮(NH4+-N≥300mg/L)和调节曝停比至1:3,可有... 详细信息

以短程硝化恶化的全程自养脱氮工艺(canon)污泥为研究对象,在间歇曝气序批式反应器(SBR)中开展自养脱氮运行效果的恢复调控研究,并基于微生物群落结构变化探究微生物调控机理.结果表明,高氨氮(NH4+-N≥300mg/L)和调节曝停比至1:3,可有效抑制亚硝酸盐氧化菌(NOB).稳定后期,亚硝酸盐氧化率(NOR)逐渐降至2.30g N/(m^3·h),ΔNO3^--N/ΔNH4^+-N逐渐降至0.13,总氮去除率(TNRR)提高到0.35kg N/(m^3·d),后延长厌氧段时间至90min,可使NOR进一步下降接近0.16S rDNA高通量测序结果表明,从恢复阶段初到稳定阶段,检测到NOB主要菌属为Nitrospira,其相对丰度从3.96%降至0.64%,CandidatusJettenia菌属(属于厌氧氨氧化菌(An AOB))相对丰度从46.68%上升至49.98%.可见,通过短期提高进水氨氮浓度可使NOB得到有效抑制,并逐渐被淘汰,使An AOB得到富集,AOB相对丰度稳态,canon系统得到恢复.

关键词： canon SBR反应器恢复稳定高通量测序

侧流FNA处理的canon工艺恢复策略及脱氮路径

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中国环境科学 2022年第10期42卷 4588-4595页

作者：任志强李冬张杰北京工业大学水质科学与水环境恢复工程北京市重点实验室北京100124 哈尔滨工业大学城市水资源与水环境国家重点实验室黑龙江哈尔滨150090

为探究侧流游离亚硝酸(FNA)处理后的颗粒-絮体污泥全程自养脱氮(canon)工艺重新建立的有效策略,采用序批式反应器(SBR)进行实验,探讨不同恢复方式对系统长期运行性能的影响,实现canon工艺长期稳定运行.结果显示,不同恢复方式对canon系... 详细信息

为探究侧流游离亚硝酸(FNA)处理后的颗粒-絮体污泥全程自养脱氮(canon)工艺重新建立的有效策略,采用序批式反应器(SBR)进行实验,探讨不同恢复方式对系统长期运行性能的影响,实现canon工艺长期稳定运行.结果显示,不同恢复方式对canon系统重新建立有很大影响,采用非原位高曝气恢复策略的R1经过19d的运行重新建立了稳定的canon工艺,而采用原位低曝气恢复策略的R2和原位高曝气恢复策略的R3均未能有效重新建立稳定的canon工艺.R1稳定运行34d时再次出现了亚硝酸盐氧化菌(NOB)增殖的现象.定期水力筛分排出絮状污泥进行FNA处理,达到溶解氧(DO)控制+侧流FNA处理的“双抑制”,能够有效抑制NOB实现canon工艺的长期稳定运行.典型周期分析结果表明,在恢复过程中,R1内氨氧化菌(AOB)、厌氧氨氧化菌(AnAOB)活性均高于R2和R3,并且有效抑制了反应器内残余NOB的活性.随着R1中canon工艺的重新建立,亚硝化/厌氧氨氧化(SNA)脱氮路径占比由第15d的8.91%增加到了第45d的19.39%,提高了NH_(4)^(+)-N的去除率.

关键词： FNA 颗粒-絮体污泥 canon 恢复策略 NOB 脱氮路径

canon系统中4种脱氮相关菌丰度和多样性

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应用基础与工程科学学报 2016年第5期24卷 863-877页

作者：高景峰李婷樊晓燕潘凯玲吴桂霞张树军北京工业大学环境与能源工程学院北京100124 北京城市排水集团有限责任公司北京100022

全程自养脱氮(Completely Autotrophic Nitrogen removal Over Nitrite,canon)是适用于低碳源、高氨氮水质的极具应用前景的新工艺,研究其中脱氮微生物种群和数量特征具有重要意义.利用PCR、克隆、实时荧光定量PCR等分子生物学技术,研... 详细信息

全程自养脱氮(Completely Autotrophic Nitrogen removal Over Nitrite,canon)是适用于低碳源、高氨氮水质的极具应用前景的新工艺,研究其中脱氮微生物种群和数量特征具有重要意义.利用PCR、克隆、实时荧光定量PCR等分子生物学技术,研究了小试(Lab-scale,L)和中试(Pilot-scale,P)2个canon系统中4种与脱氮相关的细菌(分别是氨氧化细菌(Ammonia-Oxidizing Bacteria,AOB)、亚硝酸盐氧化菌(Nitrite-Oxidizing Bacteria,NOB)、反硝化细菌(De Nitrifying Bacteria,DNB)和厌氧氨氧化细菌(ANaerobic AMMonium Oxidation,Anammox))的数量和种属,并研究了污泥形态对这4种菌的数量影响.结果发现,(1)L和P系统中AOB、NOB、DNB和Anammox菌数量均值分别处于1010、109、108和1010copies/g(以干污泥计)数量级.AOB和Anammox菌是canon系统的优势菌,而NOB和DNB数量较少.(2)AOB、NOB、DNB和Anammox菌多样性不同:DNB种属最丰富,10多个属划分为5大簇;其次是Anammox菌,主要划分为Candidatus Brocadia和Candidatus Kuenenia 2簇;再次是AOB,集中在Nitrosomonas属中的***和*** 2小簇;最后是NOB,它的种属最为单一,仅含Nitrospira marine小簇.(3)污泥形态不同影响这4种菌数量:絮体污泥更适合AOB生长,生物膜更适合DNB和Anammox菌生长,但不能确定哪种形态的污泥更适合NOB生存.研究结果将为canon系统的脱氮机理揭示和工艺优化提供理论支撑.

关键词： canon 脱氮厌氧氨氧化多样性丰度

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canon生物膜载体储存及活性恢复研究

中国环境科学 2020年第5期40卷 2062-2072页

作者：韩文杰吴迪周家中皇甫小留李果何强阚渝姣青岛思普润水处理股份有限公司生物膜研究院山东青岛266555 重庆大学三峡库区生态环境教育部重点实验室重庆400045 山东科技大学安全与环境工程学院山东青岛266590

采用不同活性恢复手段研究了短期低温(20d,15℃)储存及常温(25℃)低负荷运行后,canon-MBBR反应器的活性恢复效果.结果显示,canon悬浮载体经短期低温储存后,通过先恢复温度后缓慢提高进水负荷的方式可使其TN去除负荷恢复率达到102%.若在... 详细信息

采用不同活性恢复手段研究了短期低温(20d,15℃)储存及常温(25℃)低负荷运行后,canon-MBBR反应器的活性恢复效果.结果显示,canon悬浮载体经短期低温储存后,通过先恢复温度后缓慢提高进水负荷的方式可使其TN去除负荷恢复率达到102%.若在温度未恢复的基础上直接将进水负荷提升至原水平运行,canon悬浮载体生物膜将无法维持正常的EPS含量而易发生脱膜现象,致使TN去除负荷恢复率仅为84%.另外,系统有一定基质浓度的基础上,经过25d常温(25℃)低负荷SBR运行后,通过同样的恢复手段可使TN去除负荷恢复率达到96%.通过微生物菌群分析,确定canon-MBBR系统中功能微生物优势菌属分别为Nitrosomonas和Candidatus Kuenenia,采取合适的恢复手段使系统进入稳定运行阶段后,两者丰度均可分别达到11%以上及27%以上,且悬浮载体脱膜并未对微生物组成造成明显影响.

关键词： canon MBBR 悬浮载体储存低负荷运行活性恢复