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Transcriptome and genome analysis to identify c2h2 genes participating in low-temperature conditioning-alleviated postharvest chilling injury of peach fruit

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Food Quality and Safety 2022年第4期6卷 500-509页

作者： Yaqin Zheng Zhenning Liu hui Wang Wenna Zhang Shuaijie Li Meng Xu college of Agriculture and Forestry Sciences Linyi UniversityLinyiChina Shandong(Linyi)Institute of Modern Agriculture Zhejiang UniversityLinyiChina

Objectives:This work intended to identify candidate c2h2 genes participating in low-temperature conditioning(LTc)-alleviated postharvest chilling injury of peach fruit.Materials and Methods:For LTc treatment,fruit were pre-stored at 10℃for 5 d and then transferred to 0℃storage.Fruit firmness was measured by a hardness tester.h_(2)O_(2)content was determined by luminosity measurement model using a multifunctional enzyme labeler.Identification of c2h2 family members was performed by hMMScAN according to peach genome.The cis-acting element of gene promoters was analyzed using the Plant cARE website.Weighted gene coexpression network analysis(WGcNA)was performed by the WGcNA package in the BMK cloud platform.Results:LTc treatment decreased flesh browning rate and h_(2)O_(2)production of‘Beijing No.9’peach.Transcription factor identification of differentially expressed genes in 0℃and the LTc treatment indicated that peach c2h2 participated in the regulation of chilling injury.A total of 47 c2h2 genes were identified based on peach genome.Real-time quantitative polymerase chain reaction(qRT-PcR),phylogenetic analysis and promoter cis-acting element analysis revealed that ZFP21 was involved in the regulation of LTc-alleviated chilling injury in peach.WGcNA and dual luciferase assay suggested that ZFP21 participated in LTc-alleviated chilling injury by downregulating the expression of reactive oxygen species-related genes Rboh.conclusions:Our investigation,based on genome and RNA-seq,revealed that ZFP21 was involved in LTc treatment-alleviated chilling injury of peach fruit.This work is useful for the identification of peach cold tolerance-related genes and the study of c2h2 family in peach.

关键词： Peach fruit low-temperature conditioning chilling injury c2h2 cold storage

Direct growth of graphene on gallium nitride using c2h2 as carbon source

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Frontiers of physics 2016年第2期11卷 97-102页

作者：王兵赵云伊晓燕王国宏刘志强段瑞飞黄鹏王军喜李晋闽 Semiconductor Lighting Technology R&D center Institute of Semiconductors Chinese Academy of Sciences Beijing 100083 China

Growing graphene on gallium nitride （GaN） at temperatures greater than 900℃ is a challenge that must be overcome to obtain high quality of GaN epi-layers. We successfully met this challenge using c2h2 as the carbon source. We demonstrated that graphene can be grown both on copper and directly on GaN epi-layers. The Raman spectra indicated that the graphene films were about 4-5 layers thick. Meanwhile, the effects of the growth temperature on the growth of the graphene films were systematically studied, and 830℃ was found to be the optimum growth temperature. We successfully grew high-quality graphene films directly on gallium nitride.

关键词： graphene c2h2 gallium nitride chemical vapor deposition Raman spectroscopy

c_2h_2 sensing at V_1+V_3 band with a hollow-core photonic bandgap fiber

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Optoelectronics Letters 2011年第6期7卷 463-465页

作者：景磊姚建铨黄晓慧陆颖 college of precision instruments and optoelectronic engineering Tianjin University Key laboratory of Optoelectronic Information Technology Ministry of Education of China Tianjin University

In this paper, we demonstrate the acetylehe (c2h2) sensor with high sensitivity using a hollow-core photonic bandgap fiber (hc-PcF). Experiments for measuring c2h2 concentrations in gas mixture are performed. Using a 2 m-long hc-PcF as gas cell, the spectrum of acetylene at n1+n3 band has been measured, and the P11-branch has been selected for the purpose of sensing. A minimum detectivity of 143 parts per million by volume (ppmv) for the system configuration is estimated.

关键词：光子带隙光纤光纤传感乙炔空心气体混合物 c2h2 高灵敏度 PcF

Additivity Rule for Electron-Molecule Total cross Section calculations at 50-5000 eV： A Geometrical Approach

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chinese Physics Letters 2007年第10期24卷 2819-2822页

作者：施德恒孙金锋马恒朱遵略杨向东 college of Physics and Information Engineering Henan Normal University Xinxiang 453007 Institute of Atomic and Molecular Physics Sichuan University Chengdu 610065

To quantify the changes of the geometric shielding effect in a molecule as the incident electron energy varies, we present an empirical fraction, which represents the total cross section （TcS） contributions of shielded atoms in a molecule at different energies. Using this empirical fraction, a new formulation of the additivity rule is proposed. Using this new additivity rule, the TcSs for electron scattering by cO2, c2h2, c6h12 （cyclo-hexane） and csh16 （cyclo-octane） are calculated in the range 50-5000 e V. here the atomic cross sections are derived from the experimental TcS results of simple molecules （h2, O2, cO）. The quantitative TcSs are compared with those obtained by experiments and other theories, and good agreement is attained over a wide energy range.

关键词： POLYATOMIc-MOLEcULES ScATTERING IMPAcT c2h2 cO ENERGIES ATOMS hcN

h2 production by ethanol decomposition with a gliding arc discharge plasma reactor

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Frontiers of chemical Science and Engineering 2013年第2期7卷 145-153页

作者： Baowei WANG Wenjie GE Yijun LV Wenjuan YAN Key Laboratory for Green chemical Technology of the Ministry of Education School of Chemical Engineering and Technology Tianjin University Tianjin 300072 China

一个滑行的弧分泌物(GRD ) 反应堆被用来把乙醇分解成首先有 ch4， c2h2， c2h4，和 c2h6 的小数量的 h2 和公司。乙醇集中，电极差距，输入电压和 Ar 流动率都影响了乙醇的变换，结果从 40.7% ～ 58.0%。有趣地，为所有试验性的条件， ... 详细信息

一个滑行的弧分泌物(GRD ) 反应堆被用来把乙醇分解成首先有 ch4， c2h2， c2h4，和 c2h6 的小数量的 h2 和公司。乙醇集中，电极差距，输入电压和 Ar 流动率都影响了乙醇的变换，结果从 40.7% ～ 58.0%。有趣地，为所有试验性的条件， Sh2/ScO 选择比率在约 1.03 点是相当稳定的。为乙醇的分解的机制也被描述。

关键词：等离子体反应器乙醇浓度滑动弧放电 c2h2 分解生产 c2h4 电极间隙

Preparation of carbon Nanotubes with high Yield and Narrow Diameter Distribution from c_2h_2 over Lacu_(0.2)Ni_(0.8)O_x

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chinese chemical Letters 2004年第2期15卷 231-233页

作者： changXingTANG MeiZhenQU BoLanZhANG ZuoLongYU chengduInstituteofOrganicchemistry ChineseAcademyofSciencesChengdu610041

carbon nanotubes (cNTs) were prepared by decomposition of c2h2 over newly developed Lacu0.2Ni0.8Ox in the temperature range from 600 to 850℃. The effect of the reaction temperature on the yield of cNTs was investigated in detail. At 680℃,the yield of cNTs reaches 17 g/g.catal. or so. The morphology of cNTs was examined by TEM. The diameter of cNTs rangs from 9 nm to 14 nm.

关键词： cNTs c2h2 Lacu0.2Ni0.8Ox .

陆地棉c2h2型锌指蛋白全基因组鉴定及表达分析

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分子植物育种 2022年

作者：唐玉萍彭凡嘉郭莉莉赵瑞元郑巨云张志刚陈浩东李玉军李彩红梅正鼎湖南农业大学农学院湖南省棉花科学研究所新疆农业科学院

c2h2锌指结构蛋白(c2h2 zinc finger protein)广泛参与植物器官形态发生、激素信号转导、逆境响应等事件，在非生物逆境应激响应中发挥着重要作用。为明确陆地棉(Gossypium hirsutumL.) c2h2锌指蛋白的概况、功能及其对环境胁迫的应激... 详细信息

c2h2锌指结构蛋白(c2h2 zinc finger protein)广泛参与植物器官形态发生、激素信号转导、逆境响应等事件，在非生物逆境应激响应中发挥着重要作用。为明确陆地棉(Gossypium hirsutumL.) c2h2锌指蛋白的概况、功能及其对环境胁迫的应激响应特征，本研究通过拟南芥(Arabidopsis thaliana L.) c2h2锌指蛋白序列的Blast映射及结合c2h2锌指结构基因家族pfam从陆地棉基因组数据库筛选到343个棉花(Gossypium spp)c2h2型锌指蛋白家族成员，并对其进行了系统进化分析、基因结构、调控序列元件及组织表达分析。结果表明，棉花c2h2型锌指蛋白家族成员可分为4个亚群，且在A和D基因组的26条染色体上均有分布。4个亚类成员在基因结构、功能、组织表达特异性和逆境响应上也呈现出显著差异。此外，发现16个Q型转录因子分别在根、茎、叶等组织中呈现明显的表达差异，且多数成员表达水平在高温、盐或重金属镉逆境胁迫下差异显著，是非生物逆境应激响应过程的重要参与者。陆地棉c2h2锌指蛋白家族成员的特征分析及其差异呈现将有助于其参与的逆境响应分子机制的进一步解析，为抗逆基因的挖掘以及c2h2锌指蛋白在棉花抗逆育种中的利用提供重要启示作用。

关键词：棉花 c2h2 基因家族表达分析

Mutagenesis at a-423^(Ile) of MoFe protein reduces the catalytic activity of nitrogenase in Klebsiella oxytoca

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chinese Science Bulletin 2014年第9期59卷 849-856页

作者： Qingjuan Guo Tao Peng Tianju chang Gang Zhang Wei Jiang Ying Li Jilun Li State Key Laboratory for Agrobiotechnology and college of Biological Sciences China Agricultural University

Nitrogenase catalyzes the ATP-dependent reduction of dinitrogen to ammonia,the central process of biological nitrogen fixation.The mechanism of electron transfer from the P-cluster to the iron-molybdenum cofactor(FeMo-co)during the process of substrate reduction remains unclear.Based on our previous hypothesis,the dual pathway model,three mutants of Klebsiella oxytoca strain M5al(termed Nb102A,Ka431h and Ia423P)were constructed to map the h?/e-transfer pathway,in which b-102Asn,a-431Lys and a-423Ile of the molybdenum-iron(MoFe)protein of nitrogenases were replaced by alanine,histidine and proline,respectively.All three substitutions affected the diazotrophic growth,especially of the Ia423P strain with very low capability of diazotrophic growth and c2h2reduction activity.In a 42-L fermenter,the Ia423P strain was induced to the maximal c2h2reduction activity,which was only*17%of that of M5al.however,the transcription level of the nifD gene was approximately four-fold higher than that of the M5al strain,indicating that the low c2h2reduction activity of Ia423P was not because of downregulation of nifD expression.The maximal c2h2and h?reduction activities of the Ia423P MoFe protein were 13%and 21%of those of the wild-type MoFe protein,respectively.In conclusion,we propose that the changed properties of the Ia423P MoFe protein are related to the disruption of hydrogen bond formation between a-423Ile and homocitrate,and that a-423Ile serves as a gate to the Mo site in FeMo-co during the h?/e-transfer in nitrogenase.

关键词：钼铁蛋白固氮酶催化活性产酸乙炔还原活性诱变 c2h2 双通道模式

百香果c2h2型锌指蛋白家族特征分析及PeZAT10在寄主中对病毒的作用研究

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百香果C2H2型锌指蛋白家族特征分析及PeZAT10在寄主中对病毒的作...

作者：杨汉志福建农林大学

学位级别：硕士

鸡蛋果(passion fruit)又名百香果,因风味独特而备受消费者喜爱。病毒病是影响百香果产量和品质的最重要因素之一,严重威胁百香果产业的健康发展。c2h2型锌指蛋白是一个庞大的转录因子家族,在植物抵御生物和非生物胁迫过程中发挥重要作... 详细信息

鸡蛋果(passion fruit)又名百香果,因风味独特而备受消费者喜爱。病毒病是影响百香果产量和品质的最重要因素之一,严重威胁百香果产业的健康发展。c2h2型锌指蛋白是一个庞大的转录因子家族,在植物抵御生物和非生物胁迫过程中发挥重要作用,但关于百香果c2h2基因家族的特征及其在病毒侵染中的作用研究目前还未见报道。本文首先对百香果c2h2型锌指蛋白基因家族成员(PeZFPs)进行了生物信息学分析,并研究了它们在病毒侵染条件下的表达特征;随后选择病毒侵染后大幅上调的PeZAT10,研究了该基因在百香果与病毒互作中的作用,主要研究结果如下:(1)百香果c2h2基因家族的基本特征及其对病毒侵染的响应:百香果c2h2基因家族拥有124个成员,其中122个分布于实验室前期组装的百香果的9条染色体上,2个未被组装到染色体。百香果c2h2基因家族可以分为四个簇(cluster 1-4)。cluster 1成员普遍没有内含子,cluster 2和3成员普遍有1或2个内含子,而cluster 4成员内含子数量从0到10不等。共线性分析和染色体定位结果表明,c2h2基因家族内部发生了39次共线性复制事件。该基因家族启动区元件可分为胁迫响应类,激素调节类和生长发育类三种,其中胁迫响应类元件数量最多。分析实验室前期获得的转录谱发现,124个c2h2型锌指蛋白基因中,有12个在病毒侵染后显著上调,其中PeZFP074(PeZAT10)、PeZFP110上调幅度最大。(2)PeZFP074(PeZAT10)在病毒侵染中的作用研究:PeZAT10拥有两个典型的锌指结构,一个核定位信号区(B-box),一个转录抑制活性区(DLN-box)及一个可能富含亮氨酸(Leu)的区域(L-box)。将PeZAT10构建到p GBKT7载体,发现转化p GBKT7-PeZAT10的酵母可在SD/-T/-h/-A缺陷培养基上生长,说明PeZAT10具有转录激活活性。在本氏烟上同时接种表达PeZAT10和携带黄瓜花叶病毒(cucumber mosaic virus,cMV)或烟草花叶病毒(tobacco mosaic virus,TMV)侵染性克隆的农杆菌,然后以q RT-PcR和Northern Blot检测病毒积累水平,发现PeZAT10显著促进病毒侵染。同样的实验发现PeZAT10双锌指结构突变体丧失了促进病毒积累的能力。此外,PeZAT10蛋白的N端和c端分别有一个磷酸化位点,对两个位点进行突变,进行同样的病毒接种实验,发现突变体也丧失了促进病毒侵染的能力。为进一步验证PeZAT10在百香果与病毒互作的作用,本文在本氏烟上沉默了PeZAT10的同源基因Nb ZAT10,并获得了拟南芥At ZAT10的敲除突变体。研究发现,沉默Nb ZAT10的本氏烟及敲除At ZAT10的拟南芥对cMV的感病性均明显降低。综上,本文揭示了百香果c2h2型锌指蛋白基因家族的基本特征,研究了它们对病毒侵染的响应情况,并探讨了PeZAT10在百香果与病毒互作中的作用。所得结果对深入研究百香果c2h2型锌指蛋白基因家族的功能及百香果病毒病防控具有重要参考意义。

关键词： c2h2 百香果病毒病毒侵染锌指蛋白PeZAT10