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Down-regulation of c-type natriuretic peptide receptor by vasonatrin peptide in cardiac myocytes and fibroblasts

Acta Pharmacologica Sinica 2004年第4期25卷 26-32页

作者： Shun-yan Lü, Miao-zhang ZHU2, Dian-shi WANG3, Jun YU, Hai-tao GUO, Yu-zhen HU, Qi-ming WEI4 Department of Physiology, Departmentof Anatomy, Fourth Military Medical University, Xi-an 710032, china 4cardiorenal 3 Molecular Laboratory, University of Maryland School of Medicine, Baltimore21201, USA Department of Physiology Fourth Military Medical University Xi'an 710032 cardiorenal Molecular Laboratory University of Maryland School of Medicine Baltimore 21201 美国

AIM: To investigate the regulatory effects of vasonatrin peptide (VNP) on the expression of c-type natriuretic peptide receptor (NPR-c) in cultured neonatal rat cardiac myocytes and fibroblasts. METHODS: Quantitative RT- PcR was undertaken to evaluate the levels of NPR-c mRNA and radioimmunoassay was used to determine the formation of intracellular cGMP. RESULTS: Twenty-four hours hypoxic exposure increased the level of NPR-c mRNA in cardiomyocytes, while did not alter the expression of NPR-c in cardiac fibroblasts. VNP (1×10-8-1×10-6 mol/L) reduced the levels of NPR-c mRNA in cardiac myocytes induced by hypoxia in a concentration-dependent manner, and with high concentration (1×10-6mol/L) also decreased the expression of NPR-c in cardiac fibroblasts and air-control cardiac myocytes. The inhibitory effects of VNP on the expression of NPR-c was mimicked by 8- bromo-cGMP 1×10-6 mol/L (a membrane permeable analog of cGMP). VNP (1×10-8-1×10-6mol/L) increased the formation of intracellular guanosine-3',5'-cyclic monophosphate (cGMP) in both cardiac myocytes and fibroblasts. HS-142-1, the particulate guanylyl cyclase-coupled receptor antagonist, partially abrogated the above effects of VNP. cONcLUSION: Hypoxic exposure for 24 h up-regulated the expression of NPR-c in cultured neonatal rat cardiac myocytes. VNP decreased the expression of NPR-c in cardiac myocytes and fibroblasts under both air- control and hypoxic condition, which was at least partially mediated by guanylate cyclase linked natriuretic peptide receptors through increasing the intracellular cGMP.

关键词： c-type natriuretic peptide myocardium fibroblasts hypoxia

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The role of c-type natriuretic peptide in rat testes during spermatogenesis

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Asian Journal of Andrology 2011年第2期13卷 275-280页

作者： Dong-Hui Huang Shi-Wei Zhang Hu Zhao Ling Zhang Family Planning Research Institute Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China centre Laboratory Zhongshan Hospital ofHubei Province Wuhan 430033 China Department of Human Anatomy Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

c-type natriuretic peptide （cNP） is a 22-amino acid peptide and act as a local paracrine or autocrine regulator. There is growing evidence that chIP is involved in male reproductive processes. To investigate the role of cNPduring spermatogenesis, we measured the mRNA expression of cNPand its specific membrane-bound natriuretic peptide receptor-B （NPR-B） using real-time RT-PcR in the testes of normal rats on different postnatal days. After that spermatogenesis dysfunction model induced by ornidazole was established with the aim to study the correlation of cNPwith spermatogenic dysfunction. Then, Sertoli cells from 18- to 22-day-old healthy male rats were cultured in the presence of different cNPconcentrations （1 ×10-6, 1×10-7 and1×10-8 mol l-1）, and the mRNA expression levels of *** protein, inhibin B and transferrin were examined at 0 min, 30 min, 1 h, 2 h, 4 h, 8 h, 12 h, 24 h and 48 h. During the postnatal development of rat testes, the highest mRNA expression levels of cNPand NPR-B were found at postnatal Do, and the levels then declined gradually, with a second chIPpeak at postnatal D35. In the ornidazole-induced infertile rat testes, cIVPgene expression was lower than in the uninduced rats （P〈0.05）, while IVPR-Bgene expression was greater （P〈0.05）. In cultured Sertoli cells, supplementation with cNP stimulated the gene expression of androgen-binding pmteirginhibin B/transferrin, particularly at 12 h, and 1× 10-7 mol l-1 cNP had the highest upregulation effect. The gene expression levels of cNPIIVPR-B in rat testes at different postnatal stages and in infertile rat testes indicated that cNP may participate in the physiology and/or pathology related to spermatogenesis. Moreover, chIP regulated endocrine function in Sertoli cells. Taken together, these results showed that cNP is closely tied to spermatogenesis.

关键词： c-type natriuretic peptide natriuretic peptide receptor-B Sertoli cell culture spermatogenesis

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Role of c-type natriuretic peptide in the function of normal human sperm

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Asian Journal of Andrology 2016年第1期18卷 80-84页

作者： Hui Xia Yao chen Ke-Jia Wu Hu Zhao cheng-Liang Xiong Dong-Hui Huang Family Planning Research Institute Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China People's Hospital of Luoshan county Xinyang 464200 China Department of Human Anatomy Tongji Medical College Hua Zhong University of Science and Technology Wuhan 430030 China

c-type natriuretic peptide （cNP） is a newly discovered type of local regulatory factor that mediates its biological effects through the specific, membrane-bound natriuretic peptide receptor-B （NPR-B）. Recent studies have established that cNP is closely related to male reproductive function. The aims of this study were to determine the distribution of cNP/NPR-B in human ejaculated spermatozoa through different methods （such as immunolocalization, real time polymerase chain reaction and Western Blot）, and then to evaluate the influence of cNP on sperm function in vitro, such as motility and acrosome reaction. Human semen samples were collected from consenting donors who met the criteria of the World Health Organization for normozoospermia. Our results show that the specific receptor NPR-B of cNP is localized in the acrosomal region of the head and the membrane of the front-end tail of the sperm, and there is no signal of cNP in human sperm. compared with the control, cNP can induce a significant dose-dependent increase in spermatozoa motility and acrosome reaction. In summary, cNP/NPR-B can affect sperm motility and acrosome reaction, thus regulating the reproductive function of males. cNP may be a new key factor in regulating sperm function.

关键词： acrosome reaction c-type natriuretic peptide natriuretic peptide receptor-B sperm motility

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Regulation of c-type natriuretic peptides and natriuretic peptide Receptor-B Expression in Diabetic Rats Renal Treated by Tongluo Recipe(通络方)

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chinese Journal of Integrative Medicine 2013年第7期19卷 524-531页

作者：曲卫姜兆顺张春阳邹俊杰孙亮亮石勇铨刘志民 Department of Endocrinology General Military Hospital of Jinan Military Region Department of Endocrinology Tongji Hospital Department of Endocrinology Changzheng HospitalSecond Military Medical University

To investigate the expression of c-type natriuretic peptides （cNP） and natriuretic peptide receptor-B （NPR-B） receptor in diabetic rats renal cortex, and the regulation by Tongluo Recipe （通络方, TLR）. Methods： Sixty male SD rats were divided into 3 groups： the normal control group, diabetic model group and diabetic TLR group. Each group was further divided into two subgroups of ten in each, according to 4-week or 12-week observation period. Streptozotocin （STZ）-induced diabetic rats were treated with TLR （1.0 ***＇d1） for 4 and 12 weeks, respectively. （1） The essential information was collected for comparing renal mass, serum creatinine and 24 h urine albumen on each group was calculated. （2） cNP mRNA and NPR-B mRNA were detected by realtime-polymerase chain reaction （PcR） on rats renal cortex. （3） concentration of cNP on renal cortex or serum were analyzed by enzyme-linked immunosorbent assay （ELISA）. （4） Pathological evaluation and NPR-B immunostaining for renal tissue were also performed. Results：（1） cNP and NPR-B mRNA levels were detected in each treated or untreated group, with slight elevated in untreated diabetes rats administrated with STZ after 4-week and cNP mRNA level remarkable elevated at 39.21 times higher than normal control group after 12 weeks, but NPR-B mRNA level showed a remarkably down-regulation at 98.07% after 12 weeks. cNP mRNA of TLR-treated group was also elevated after 12-week treatment, but less than untreated group. （2） concentrations of cNP in renal cortex were obviously increased in treated or untreated diabetes rats, within these groups the treatment of TLR was found more significantly on prompting cNP concentration. comparing to normal group, serum concentrations of cNP were also increased in treated or untreated diabetic groups, but there was no difference between these diabetic groups. （3） Renal lesions like glomerular volume increased are observed mostly in the relative eady stage after 4 weeks. Although TLR treated group had no s

关键词： diabetic nephropathy rat c-type natriuretic peptide natriuretic peptide receptor-B Tongluo Recipe

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Research Progress of In vitro Oocyte Maturation

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Reproductive and Developmental Medicine 2021年第3期5卷 183-192页

作者： Yuan-Xue Jing Yi-Qing Wang Hong-Xing Li Feng Yue Shi-Long Xue Xue-Hong Zhang Reproductive Medicine center the First Hospital of Lanzhou UniversityLanzhou 730000China Key Laboratory for Reproductive Medicine and Embryo of Gansu Lanzhou 730000China The First School of clinical Medicine Lanzhou UniversityLanzhou 730000China

In vitro maturation(IVM)has been used in clinical settings for 30 *** merits of IVM include that it needs a relatively small amount of hormones and short treatment ***,because the effectiveness of IVM is lower than that of controlled ovarian hyperstimulation,there are few centers routinely use IVM,and it is only applicable to a few special *** this article,several oocyte sources related to IVM have been discussed and the effects of gonadotropin priming and triggering on IVM are ***,we have reviewed the optimization of IVM culture conditions in recent years along with the effects of IVM on genes of oocytes and cumulus cells and the obstetric and neonatal *** aim to provide indications for future improvement of IVM technology so that the success rates of IVM technology in special populations can be *** hope that this mild and natural protocol can be applied to more populations,including individuals with normal ovulation.

关键词： c-type natriuretic peptide cyclic Adenosine Monophosphate Epidermal Growth Factor-Like peptides In vitro Maturation Melatonin Oocyte

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