检索结果-南通市图书馆

检索条件"主题词=Cα–Cβ bond cleavage"

共 1 条记录，以下是1-10 订阅

全选清除本页清除全部题录导出标记到"检索档案"

详细简洁

排序：

cα–cβ bond cleavage Occurs at the Side chain of the N-Terminal Phenylalanine Residue During cID of Tyrosine-containing Peptide Radical cations

Cα–Cβ Bond Cleavage Occurs at the Side Chain of the N-Ter...

引用

2018年中国质谱学术大会（cMSc2018）

作者： Xiaoyan Mu Wai Kit Tang Naiping Dong Mengzhu Li chi-Kit Siu Ivan K.chu Department of chemistry University of Hong Kong Department of chemistry City University of Hong Kong

Molecular radical cationic peptides(M) display rich and diverse gas-phase ion chemistry that can differ substantially from that of their protonated counterparts. Dissociation of M radical cations can provide relevant structural information for protein sequencing, often complementary to that obtained using traditional approaches1-2. In this study, low-energy collision-induced dissociation(cID) of a series of tyrosine-containing peptide molecular radical cations having an N-terminal phenylalanine residue led to new products resulting from radical-mediated c–c bond cleavage at the side chain of the phenylalanine residue. The chemical identity of the species formed through side chain loss(91 Da) occurring via N-terminal c–c bond cleavage was examined using a combination of lowenergy cID experiments, isotopic labeling mass spectrometric experiments, and density functional theory calculations. This unusual dissociation process occurs with fascinating unimolecular gas phase ion chemistry and requires a π-radical cationic tyrosine residue with significant spin density delocalized into the N-terminal amino group. The radical character at the resulting –NH moiety then induces β-scission at the side chain c–c bond of the phenylalanine residue. This unique neutral radical loss(91 Da) from the N-terminal phenylalanine residue, observed here for the first time, should provide additional sequence information for biological tandem mass spectrometry. This discovery also demonstrates the role of the NH group in facilitating c–c bond cleavage of the phenylalanine residue by the proximal tyrosine π-radical cation, probably involving a through-space electron transfer process.

关键词： Radical-site migration hydrogen atom transfer proton transfer cα–cβ bond cleavage 91 Da loss

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

全选清除本页清除全部题录导出标记到“检索档案”

共1页 << < 1 > >>

回到顶部

执行限定条件

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：