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Molecular characterization and functional analysis of daf-8 in the pinewood nematode,bursaphelenchus xylophilus

Journal of Forestry Research 2022年第2期33卷 689-698页

作者： Jinghan Wang Huan Hong Rong Xie Jingjing Ji Kai Guo Liqun Bai Jia Tang Hongshi Yu Jianren Ye Jiafu Hu Nanjing Forestry University Nanjing 210037China Zhejiang Agricultural and Forestry University Hangzhou 311300China Yuhuan Natural Resources and Planning Bureau Taizhou 317600China School of BioSciences The University of MelbourneParkvilleVIC 3010Australia

bursaphelenchus xylophilus,causal agent of pine wilt disease,causes extensive damage ***-gies are needed to inhibit population growth or block the spread of the invasive nematode to control pine wilt *** gene daf-8 plays crucial roles in larval develop-ment and dauer formation in Caenorhabditis elegans,but little is known about its orthologue in *** the present molecular characterization and functional analysis of daf-8 in ***(Bx-daf-8),RT-qPCR showed that the expression of Bx-daf-8 gradually increased during the embryonic stage,peaked in the second-stage juvenile(J2),then dramatically dropped in the J3,and remained at that low level for the rest of its ***-daf-8-transgenic *** was employed to mimic the spatiotemporal expression of Bx-daf-8,which was expressed close to the pharynx during early embryogenesis and weakly throughout the whole body during late *** was observed in head neurons and tail ganglions throughout all postembryonic *** embryos were severely abnormal,and hatching rate decreased sharply after Bx-daf-8 ***-16-1 and da-f16-2,downstream genes in the IIS pathway,also dropped sharply after Bx-daf-8 *** propose that TGFβmay crosstalk with the IIS pathway upstream of Bx-daf-16 and that daf-8 may act as a master regulator of daf-16 in ***,knockdown of Bx-daf-8 did not lead to constitutive developmental arrest at the dauer larval stage,indicating that dauer entry in *** might be controlled by several genes and is more complicated than in ***-daf-8 alone did not control the dauer entry in ***,but it was indispensable for embryogen-esis,providing a potential target for suppressing population growth of ***.

关键词： Daf-8 Expression pattern RNAi Embryogenesis bursaphelenchus xylophilus

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Bioinformatics analysis of structure and function in the MRP gene family and its expression in response to various drugs in bursaphelenchus xylophilus

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Journal of Forestry Research 2021年第2期32卷 779-787页

作者： Jian Diao Xin Hao Wei Ma Ling Ma College of Forestry Northeast Forestry UniversityHarbin 150040People’s Republic of China Heilongjiang University of Chinese Medicine Harbin 150000People’s Republic of China

Genes homologous to members of the MRP gene family in Caenorhabditis elegans are important in drug *** further explore the molecular mechanism of drug resistance in pine wood nematode(bursaphelenchus xylophilus),we used bioinformatics approaches to analyze genomic data for *** and identified Bx-MRP *** predicted the structure and function of the genes and encoded *** bioinformatics programs to predict and analyze various properties of the predicted proteins,including hydrophobicity,transmembrane regions,phosphorylation sites,and topologically isomeric structures,of these Bx-MRP genes,we determined that they function in transmembrane *** the results of RT-qPCR,the Bx-MRP family members confer significant differential resistance to different drug *** treatment with different concentrations of emamectin benzoate,avermectin and matrine,the expression of each gene increased with increasing drug concentrations,indicating that the family members play a positive role in the regulation of multidrug resistance.

关键词： bursaphelenchus xylophilus Bx-MRP gene family Gene and protein structure Bioinformatics Multidrug stress

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Cloning of sft-4 and its influence on vitality and virulence of pine wood nematode,bursaphelenchus xylophilus

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Journal of Forestry Research 2024年第5期35卷 191-200页

作者： Shuisong Liu Linsong Wang Ronggui Li Mengyu Chen Wenjun Deng Chao Wang Guicai Du Qunqun Guo College of Life Sciences Qingdao UniversityQingdao 266071People’s Republic of China

In our previous screening of the transcriptome of the causal agent of the devastating pine wilt disease,pine wood nematode(PWN,bursaphelenchus xylophilus),after treatment with the nematicide fomepizole,Surfeit locus gene sft-4,which encodes a regulatory factor,was found to be *** situ hybridization results showed that the sft-4 was continuously expressed from egg to adult and was especially high in the reproductive *** in a study of the effect of RNA interference(RNAi)of sft-4 and recombinant SFT-4 on PWN activity,treatment with sft-4 dsRNA inhibited feeding,reproduction,oviposition and egg hatching of PWN with the greatest inhibition on reproduction and oviposition,whereas recombinant SFT-4 had the opposite *** addition,RNAi of sft-4 changed the female–male ratio and lifespan of *** bioassays of PWNs,with RNAi of sft-4 on seedlings and 2-year-old Pinus thunbergii trees,none of the treated plants developed symp-toms during the monitoring period,indicating that virulence of PWNs was either significantly *** results indicate that the influence of sft-4 on PWN pathogenicity may be mainly through regulating reproductive function of PWN and its lifespan.

关键词： Black pine Pinaceae bursaphelenchus xylophilus Sft-4 In situ hybridization RNAi Pathogenicity

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Comparative study on the mRNA expression of Pinus massoniana infected by bursaphelenchus xylophilus

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Journal of Forestry Research 2020年第1期31卷 75-86页

作者： Wanfeng Xie Guanghong Liang Aizhen Huang Feiping Zhang Wenshuo Guo Jinshan College Fujian Agriculture and Forestry UniversityFuzhouChina Institute of Forestry Protection Forestry CollegeFujian Agriculture and Forestry UniversityFuzhouChina

Pine wilt disease(PWD)is a devastating disease affecting the growth of Pinus massoniana,often leading to withering and *** reveal the changes involved during disease progression,we investigated the mRNA expression profile of *** infested by bursaphelenchus *** infestation resulted in the downregulation of genes involved in interactions with pathogenic pathways such as disease resistance gene,CC-NBS-LRR resistancelike protein,and the gene encoding a putative nematode resistance *** infestation pressure(number of nematodes inoculated)caused a continuous decline in the gene expression of stem *** infestation of *** also resulted in a pathway enrichment of genes involved in phenylpropanoid metabolism and flavonoid biosynthesis,which in turn reduced the levels of total phenols and total flavonoids.A downregulation of auxin responsive family protein was observed in infested samples,which resulted in a suppression of plant ***,upon *** infestation,a downregulation of genes associated with the recognition of pathogens,PWD resistance,and growth regulation was observed in ***,together with a decrease in the levels of phytoalexinlike secondary substances,all of which resulted in withering and ultimately death of ***.

关键词： Auxin/IAA bursaphelenchus xylophilus Pinus massoniana Resistance gene Phytoalexin

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Transcriptomic response of Pinus massoniana to infection stress from the pine wood nematode bursaphelenchus xylophilus

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Stress Biology 2023年第1期3卷 534-545页

作者： Yibo An Yongxia Li Ling Ma Dongzhen Li Wei Zhang Yuqian Feng Zhenkai Liu Xuan Wang Xiaojian Wen Xingyao Zhang Key Laboratory of Forest Protection of National Forestry and Grassland Administration Ecology and Nature Conservation InstituteChinese Academy of Forestryl00091BeijingChina Co-Innovation Center for Sustainable Forestry in Southern China Nanjing Forestry UniversityNanjing 210037China Chongqing Forestry Investment and Development Co. Ltd.National Forestry and Grassland National Reserve Forest Engineering Technology Research CenterChongqing 401120China Northeast Forestry University College of ForestryHarbin 150040China

The pinewood nematode(PWN)bursaphelenchus xylophilus is a forestry quarantine pest and causes an extremely dangerous forest disease that is spreading *** to the complex pathogenic factors of pine wood nematode disease,the pathogenesis is still *** ultimately invades a host and causes ***,little is known about the defence-regulating process of host pine after infection by *** at the molecular ***,we wanted to understand how Pinus massoniana regulates its response to invasion by *** were artificially inoculated with *** solution,while those without *** solution were used as *** inoculated with *** solution for 0 h,6 h,24 h,and 120 h was subjected to highthroughput sequencing to obtain transcriptome *** various time points(0 h,6 h,24 h,120 h),gene transcription was measured in *** inoculated with *** different time points,*** gene transcription differed significantly,with a response to early invasion by *** to Gene Ontology(GO)classification and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis,*** response to PWN invasion involves a wide range of genes,including plant hormone signal transformation,flavonoid biosynthesis,amino sugar and nucleoside sugar metabolism,and MAPK signalling *** them,inoculation for 120 hours had the greatest impact on differential ***,weighted gene coexpression network analysis(WGCNA)was used to analyse transcriptional regulation of *** after PWN *** results showed that the core gene module of *** responding to PWN was“MEmagenta”,enriched in oxidative phosphorylation,amino sugar and nucleotide sugar metabolism,and the MAPK signalling *** family transcription factors with the highest number of changes between infected and healthy pine trees accounted for 20.4% of the total differentially expressed tran

关键词： Pinus massoniana bursaphelenchus xylophilus Transcriptome WGCNA

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Research Advances in Interplay of bursaphelenchus xylophilus and Other Key Biological Factors Related to Pine Wilt Disease

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Chinese Forestry Science and Technology 2006年第3期5卷 60-66页

作者： LIU Huimin NING Shaohua LIANG Jun LU Quan Research Institute of Forest Ecology Environment and Protection Chinese Academy of Forestry Beijing 100091 P. R. China

The paper separately discusses interplay of pine wood nematode and several key biological factors related to pine wilt disease, such as host trees, insect vectors, fungi, symbiotic bacteria, and natural enemy acarid, etc. By virtue of so much complicated interplay among the biological factors related to pine wilt disease which is different from other forest disease, it also points out that the further researches about pine wilt disease should focus on interplay mechanism of key biological factors to discover pathogenic mechanism, and simple and quick inspection and quarantine methods.

关键词： bursaphelenchus xylophilus biological factors interplay

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Suggestions on management measures of pine forest ecosystems invaded by bursaphelenchus xylophilus

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Forest Ecosystems 2008年第1期10卷 45-48页

作者： SHI Juan, LUO You-qing*, XIA Nai-bin, WU Hai-wei, SONG Ji-ying Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, P. R. China Key Laboratory for Silviculture and Conservation of Ministry of Education Beijing Forestry University Beijing 100083 P. R. China

Pine wood nematode (PWN), bursaphelenchus xylophilus (Steiner et Buhrer) Nickle is an important invasive alien species in forests of China and has become one of the most destructive forest diseases. In order to improve the resistance and resilience of pine forest ecosystems against B. xylophilus invasion and make the pine forest ecosystem more timely responsive to PWN invasion, we made some recommendations based on five years of intensive observations. We advocate a set of management measures with the theme "Prevention is priority, but integrated with curative techniques and ecological resilience" on the pine forest ecosystem invaded by B. xylophilus; details of accurate measures are proposed. The aim is to discover the underlying problems of present pine forest ecosystems and to take, correspondingly, administrative measures and strategies, which will encourage the pine forest ecosystem to develop in a benign way.

关键词： bursaphelenchus xylophilus pine forest ecosystem invasion management measures

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Pathogenicity of aseptic bursaphelenchus xylophilus

Pathogenicity of aseptic Bursaphelenchus xylophilus

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第三届中国森林保护学术大会

作者： Zhu Li-hua Wang Zhang-li Wu Xiao-qin Ye Jian-ren (College of Forest Resources and Environment,Nanjing Forestry University,Jiangsu Key Laboratory for Prevention and Management of Invasasion Species,Nanjing 210037)

In an attempt to elucidate the pathogenicity of the pine wood nematode and the bacterium associated with the nematodes,the tissue-cultured aseptic plantlets of Pinus densiflora were inoculated under aseptic conditions with aseptic pine wood nematodes,non-aseptic pine wood nematodes,bacterium isolates isolated from the nematodes,and sterile *** results showed that the aseptic tissue-cultured plantlets inoculated with the aseptic nematodes or non-aseptic nematodes wilted,and the wilting rate was 97%and 88%,respectively,while those inoculated with the bacterium isolates or sterile water did not *** were recovered both from wilted plantlets that inoculated with aseptic pine wood nematodes and non-aseptic pine wood *** asepsis of nematodes recovered from wilted plantlets that inoculated with aseptic pine wood nematodes were confirmed by their culture on the NA medium at 30℃for 3 ***,asepsis of the pine wood nematode did not cause it to lose its pathogenicity.

关键词： bursaphelenchus xylophilus asepsis Pinus densiflora pathogenicity

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Nitric Oxide Involved in Pinus thunbergii Reponse to the Invasion of bursaphelenchus xylophilus,and NOS Mediated the Generation of NO

Nitric Oxide Involved in Pinus thunbergii Reponse to the Inv...

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第三届全国生物入侵大会

作者： Lu-zhen Yu,Xiao-qin Wu~*,Jian-ren Ye,Sai-nan Zhang Nanjing Forestry University,The Key Laboratory of the Pest Prevention and Control of the Jiangsu Province,Nanjing 210037,China

Pine wilt disease caused by bursaphelenchus xylophilus has caused great damage to forest resource and threantened ecological environment in China in the past ***,the role of NO(nitric oxide,NO) and ROS(reactive oxygen... 详细信息

关键词： bursaphelenchus xylophilus Pinus thunbergii Nitric oxide Hydrogen peroxide Nitric oxide synthase Nitrate reductase

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Construction and analysis of SSH cDNA library of different pathogenicity strains of bursaphelenchus xylophilus

Construction and analysis of SSH cDNA library of different p...

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第三届全国生物入侵大会

作者： Xu-ling Xu,Xiao-qin Wu~*,Jian-ren Ye,Lin Huang Nanjing Forestry University,The key Laboratory of the pest prevention and control of Jiangsu Province, Nanjing 210037,China

bursaphelenchus xylophilus as the pathogen of pine wilt disease,has caused destructive damage to pine plantations and resulted in the annual loss of millions of pine trees in *** of B. xylophilus is an important factor in the *** has been reported that the pathogenicity of B. xylophilus isolated from different geographical origins has significant *** present,the molecular mechanism of pathogenicity differentiation is still *** order to screen the

关键词： bursaphelenchus xylophilus Cdna Pathogenicity SSH

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