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Factors affectingin-vivo fertility of crossbred Egyptian - Italian buffalo semen

Asian pacific Journal of Reproduction 2017年第6期6卷 264-267页

作者： GA El-Sisy KGhM Mahmoud AAE El-sokary MF Nawito YF Ahmed Department of Animal Reproduction and A.I. National Research CenterDokkiGizaEgypt General Organization for Veterinary Services DokkiEgypt

Objective:To assess the effect of region, season and year of insemination onin-vivo fertility of Italian-Egyptian crossbred buffalo ***: A total number of 4799 female buffaloes were inseminated by frozen semen with at least 50% post-thaw motility of Egyptian-Italian crossbred bulls in three localities in Delta, lower Egypt (El-Behira, El-Sharkia and Damietta) during the period of 2013, 2014 and 2015. The pregnancy rate after two months was evaluated during the four ***:The rate of pregnancy was significantly (P<0.0001) differ among the three localities. The effect of year of insemination on pregnancy rate was significantly higher during 2014 and 2015 than 2013 in El-Sharkia and El-Behira. But in Damietta, the rate of pregnancy was significantly higher in 2014 than 2013 and 2015. There were no significant differences among seasons in El-Behira and Damietta governorates but there was significant (P<0.05) differences in pregnancy rate in El-Sharkia. It was higher in summer, spring and autumn than in ***:Localities, year of insemination and season of the year have effects on fertility of crossbred Egyptian-Italian buffalo semen.

关键词： Artificial insemination Pregnancy rate Egyptian-Italian crossbred Buffalo bull

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Ergot Alkaloid Effects on Bovine Sperm Motility <i>In Vitro</i>

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Advances in Reproductive Sciences 2019年第1期7卷 7-15页

作者： Ryan Page Toby Lester Rick Rorie Charles Rosenkrans Jr. Department of Animal Science Division of Agriculture University of Arkansas System Fayetteville Arkansas USA

Cattle in some parts of the world graze pastures that consist of tall fescue that may contain ergot alkaloid contamination. Those ergot alkaloids are associated with reduced reproductive rates in cattle. Our objective was to determine if ergot alkaloids [dihydroergotamine (DHET), ergonovine (EN), and ergotamine (ET)] directly affect bovine sperm characteristics. Spermatozoa were collected from mature Angus (n = 2) and Balancer (n = 4) bulls. Within bull, treatments were structured as a 3 × 5 factorial with three alkaloids (DHET, EN, and ET) and five concentrations of each alkaloid (0, 33, 66, 100, or 200 μM). Spermatozoa (25 × 106) were incubated (39?C) in 1 mL of modified sperm medium. Sperm motility characteristics were evaluated using CASA (Hamiliton Thorne IVOS, Beverly, MA) at 0, 3, and 6 h after initial alkaloid exposure. Initial sperm motility was (69% ± 1.1%) and declined (P = 0.01) to (35% ± 2.6%) at 6 h. Sperm motility decreased (P P < 0.05) both characteristics. Our results demonstrate that ergot alkaloids (ET and DHET) can directly alter bovine sperm motility and morphology, which adds to our understanding of how ergot alkaloids may hinder cattle reproductive rates.

关键词： CASA bull Spermatozoa Toxins

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Bovine Sperm Motility as Affected by Alpha Tocopherol and Ascorbic Acid during Storage

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Advances in Reproductive Sciences 2019年第2期7卷 39-49页

作者： Ryan Page Charles Rosenkrans Jr. Department of Animal Science Division of Agriculture University of Arkansas System Fayetteville AR USA

Our purpose was to determine the impact of ascorbic acid and α-tocopherol on bovine sperm motility following short-term storage or cryopreservation. Semen was collected from mature Angus bulls (n = 4). The experimental design was a randomized complete block, with bull serving as block, and treatments were structured as a 4 × 4 factorial with four concentrations of ascorbic acid (0, 5, 10, 20 mM) and four concentrations of α-tocopherol (0, 0.05, 0.5, 5 mM). Sperm motility characteristics were evaluated using computer assisted sperm analyses at 0, 4, and 8 h of incubation (39?C) and post-cryopreservation. Initial sperm motility was (79.6% ± 1.6) and decreased to (6.1% ± 1.6%) after cryopreservation. Cryopreserved spermatozoa had lower (P P > 0.1) by α-tocopherol. Addition of 20 mM ascorbic acid to storage media decreased (P P α-tocopherol. Stepwise regression models predicted (P α-tocopherol was not beneficial for short-term storage of spermatozoa;however, our results were inconclusive with regards to inclusion of ascorbic acid and α-tocopherol in egg yolk-based cryopreservation media.

关键词： bull Spermatozoa Antioxidants Cryopreservation

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ENVIRONMENTAL GEOLOGY

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Abstracts of Chinese Geological Literature 2010年第3期26卷 162-173页

20102049 Bu Ruimei (The First China Railway Survey and Design Institute Group,Xi’an 710043) Assessment of Threatening Engineering Geohazards on the Section between Xining and Golmud as Part of the Second Phase of the... 详细信息

关键词： landslide geologic rock hazard Tibetan tables bull

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