检索结果-南通市图书馆

The effects of high temperature level on square bt protein concen-tration of bt cotton

Journal of Integrative Agriculture 2015年第10期14卷 1971-1979页

作者： WANG Jun CHEN Yuan YAO Meng-hao LI Yuan WEN Yu-jin CHEN Yuan ZHANG Xiang CHEN De-hua Jiangsu Provincial Key Laboratory of Crops Genetics and Physiology Yangzhou University Yangzhou 225009 P.R. China Department of Soil and Crop Sciences Texas A&M University College Station TX77843 USA Syngenta Biotechnology Inc. Carry NC 27519 USA

Higher boll worm survival rates were detected after high temperature presented during square period in bt cotton. The objective of this study was to investigate the effects of high temperature level on the bt efficacy of two different types of bt cotton cultivars at squaring stage. During the 2011 to 2013 cotton growth seasons, high temperature treatments ranged from 34 to 44°C in climate chambers, and field experiments under high temperature weather with various temperature levels were conducted to investigate the effects of the high temperature level on square bt protein concentration and nitrogen metabolism. The climate chamber experiments showed that the square insecticidal protein contents reduced after 24 h elevated temperature treatments for both cultivars, whereas significant declines of the square insecticidal protein contents were detected at temperature 〉38°C, and only slightly numerical reductions were observed when temperature below 38°C. Similar high temperature responses were also observed at the two field experimental sites in 2013. Correspondingly, high temperature below 38°C seems have little effect on the square amino acid concentrations, soluble protein contents, glutamic-pyruvic transaminase（GPT） and glutamic-oxalacetic transaminase（GOT） activities as well as protease and peptidase activities; however, when the temperature was above 38°C, reduced soluble protein contents, enhanced amino acid concentrations, decreased GPT and GOT activities, bolstered protease and peptidase activities in square were detected. In general, the higher the temperature is（〉38°C）, the larger the changes for the above compound contents and key enzymes activities of the square protein cycle. The findings indicated that the unstable insect resistance of the square was related to high temperature level during square stage.

关键词： bt cotton square high temperature level stress bt protein nitrogen metabolism

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Inhibition of protein degradation increases the bt protein concentration in bt cotton

引用

Journal of Integrative Agriculture 2024年第6期23卷 1897-1909页

作者： Yuting Liu Hanjia Li Yuan Chen Tambel Leila.I.M Zhenyu Liu Shujuan Wu Siqi Sun Xiang Zhang Dehua Chen Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009China Agricultural Research Cooperation Biotechnology and Biosafety Research CenterKhartoum 13314Sudan

Bacillus thuringiensis(bt)cotton production is challenged by two main problems,i.e.,the low concentration of bt protein at the boll setting stage and the lowest insect resistance in bolls among all the cotton plant’s ***,increasing the bt protein concentration at the boll stage,especially in bolls,has become the main goal for increasing insect resistance in *** this study,two protein degradation inhibitors(ethylene diamine tetra acetic acid(EDTA)and leupeptin)were sprayed on the bolls,subtending leaves,and whole cotton plants at the peak flowering stage of two bt cultivars(medium maturation Sikang 1(SK1))and early maturation Zhongmian 425(ZM425)in 2019 and *** bt protein content and protein degradation metabolism were *** results showed that the bt protein concentrations were enhanced by 21.3 to 38.8%and 25.0 to 38.6%in the treated bolls of SK1 and ZM425 respectively,while they were decreased in the subtending leaves of these treated *** the treated leaves,the bt protein concentrations increased by 7.6 to 23.5%and 11.2 to 14.9%in SK1 and ZM425,*** combined application of EDTA and leupeptin to the whole cotton plant increased the bt protein concentrations in both bolls and subtending *** bt protein concentrations in bolls were higher,increasing by 22.5 to 31.0%and 19.6 to 32.5%for SK1 and ZM425,*** organs treated with EDTA or/and leupeptin showed reduced free amino acid contents,protease and peptidase activities and significant enhancements in soluble protein *** results indicated that inhibiting protein degradation could improve the protein content,thus increasing the bt protein concentrations in the bolls or/and leaves of cotton ***,the increase in the bt protein concentration without yield reduction suggested that these two protein degradation inhibitors may be applicable for improving insect resistance in cotton production.

关键词： bt cotton bt protein inhibition of protein degradation protein degradation metabolism

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Animal Safety Test of Bacillus thuringiensis bt protein

引用

Asian Agricultural Research 2024年第2期16卷 31-32,35页

作者： Weimeng NING Dingkuo LIU Fang LIU Yuan LI Xiaowei YANG Zhenguo GUAN Guijun YANG Bo ZHANG Pengcheng HU Dingzheng Xinxing Biotechnology(Tianjin)Co. Ltd.Tianjin 300383China Tianjin Agricultural College Tianjin 300393China Tianjin Key Laboratory of Biological Feed Additives Tianjin 300383China Tianjin Feed Small Molecule protein Engineering Center Tianjin 300383China Tianjin Agricultural Ecological Environment Monitoring and Agricultural Product Quality Testing Center Tianjin 300193China

[Objectives]To determine the biological safety of bt protein from Bacillus thuringiensis(bt)fermentation broth to mammals at high doses.[Methods]Healthy mice were randomly divided into 4 groups with 10 mice in each *** experimental groups were fed with bt fermentation supernatant at 0.2,0.6 and 1.0 mL/kg,respectively,once a day for 7 consecutive *** blank control group was fed normally without intragastric administration.[Results]There was no significant difference in blood routine and blood biochemical analysis between the experimental group and the control *** intragastric administration,the mice were dissected,and no obvious pathological changes were found;the heart,liver,spleen,lung and kidney were taken to make tissue sections,and no pathological changes were found by microscopic observation.[Conclusions]Mice can tolerate high doses of bt protein from *** fermentation broth.

关键词： Bacillus thuringiensis(bt) bt protein Biological safety

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Expression of bt protein in Transgenic Pest-resistant Rice

引用

Agricultural Science & Technology 2012年第3期13卷 489-491页

作者：于志晶蔡勤安林秀峰马瑞吉林省农业科学院生物技术研究中心吉林130033

[Objective] The aim of this study was to study on expression of bt protein in transgenic pest-resistant rice. [Method] Enzyme-linked immunosorbent assay （ELISA） was used to measure bt protein expression in different tissues of transgenic pest-resistant rice at same growth stage. [Result] Absolute content of bt protein from high to low was as follows： leaves 〉 immature seeds and glumes 〉 roots 〉 stems in different tissues of transgenic rice in grain-filling stage; bt protein content of trans- genic rice changed a little in different growth stages （including tillering stage, booting stage, and grain-filling stage）; in general, its level declined a little in later growth stage, but the resistibility would not be influenced significantly. [Conclusion] The ex- periment is significant for pest prevention and transgenic rice breeding.

关键词： Enzyme-linked immunosorbent assay (ELISA) Transgenic pest-resistant rice bt protein

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Relationship Between Leaf C/N Ratio and Insecticidal protein Expression in bt Cotton as Affected by High Temperature and N Rate

引用

Journal of Integrative Agriculture 2014年第1期13卷 82-88页

作者： ZHANG Xiang LU Chun-hua CHEN Yuan WANG Gui-xia CHEN Yuan CHEN De-hua Jiangsu Provincial Key Laboratory of Crops Genetics and Physiology Yangzhou University Yangzhou 225009 P.R.China Department of Soil and Crop Sciences Texas A&M University College Station 77843 USA

Expression of insecticidal protein for transgenic Bacillus thuringiensis （bt） cotton is unstable and related to nitrogen metabolism. The objective of this study was to investigate the relationship between leaf carbon nitrogen ratio （C/N） and insecticidal efficacy of two bt cotton cultivars. C/N ratio and bt protein content were both measured at peak square period and peak boll period respectively under 5-7 d high temperature and different nitrogen fertilizer rates on the Yangzhou University Farm and the Ludong Cotton Farm, China. All plants were grown in field. The results showed that the C/N ratio enhanced slightly and the bt protein content remained stable at peak square period, but significant increases for the C/N ratio and decreases markedly for the leaf bt protein concentration were detected at the peak boll period. The similar patterns at the two growth periods were found for the leaf C/N ratio and bt protein content by different N fertilizer treatments. When nitrogen rate was from 0 to 600 kg ha-l, the C/N ratio was reduced by 0.017 and 0.006 for Sikang 1 and Sikang 3 at peak square period, compared to the 1.350 to 1.143 reduction for Sikang 1 and Sikang 3 at peak boll period, respectively. Correspondingly, the leaf bt protein contents were bolstered by 2.6-11.8 and 26.9-36.9% at the two different growth periods, respectively. The results suggested that enhanced C/N ratio by high temperature and nitrogen application may result in the reduction of inseetiocidal efficacy in bt cotton, especially in peak boll period.

关键词： bt cotton C/N ratio bt protein insecticidal efficacy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of inhibitors on the protease profiles and degradation of activated Cry toxins in larval midgut juices of Cnaphalocrocis medinalis (Lepidoptera:Pyralidae)

引用

Journal of Integrative Agriculture 2021年第8期20卷 2195-2203页

作者： YANG Ya-jun XU Hong-xing WU Zhi-hong LU Zhong-xian State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Institute of Plant Protection and MicrobiologyZhejiang Academy of Agricultural SciencesHangzhou 310021P.R.China

Midgut juice plays an important role in food digestion and detoxification in *** order to understand the potential of midgut juice of Cnaphalocrocis medinalis(Guenée)to degrade bt proteins,the enzymatic activity of midgut juice and its degradation of bt proteins(Cry2A,Cry1C,Cry1Aa,and Cry1Ac)were evaluated in this study through protease inhibitor *** activities of total protease in midgut juices were significantly inhibited by phenylmethylsulfonyl fluoride(PMSF),tosyl-L-lysine chloromethyl ketone(TLCK),pepstatin A and *** enzymatic activity of chymotrypsin was significantly inhibited by PMSF,and enzymatic activity of trypsin was significantly inhibited by ethylenediaminetetraacetic acid(EDTA),PMSF,tosyl phenylalanine chloromethyl ketone(TPCK),TLCK and trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane(E-64).EDTA could significantly inhibit the degradation of Cry2A by ***,PMSF,TPCK,and TLCK could inhibit the degradation of Cry1C and ***,PMSF,TPCK,TLCK,and E-64 could inhibit the degradation of *** results indicated that some protease inhibitors hindered various enzymatic activities in the larval midgut of ***,which may reduce the insect’s ability to degrade bt *** findings may aid the application of protease inhibitors in the management of this insect pest in the future.

关键词： Cnaphalocrocis medinalis midgut juice protease inhibitor enzyme activity degradation bt protein

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bacillus thuringiensis （bt） Toxin,Gossypol,and Condensed Tannin Contents of Transgenic bt Cotton（Gossypium hirsutum L.） Seedlings Affected by Mepiquat Chloride Application and Growth Temperature

Bacillus thuringiensis （Bt） Toxin,Gossypol,and Condensed T...

引用

中国棉花学会2005年年会暨青年棉花学术研讨会

作者： JIANG Lin-jian, DUAN Liu-sheng, TIAN Xiao-li, WANG Bao-min, ZHZNG Ming-cai, LI Zhao-hu(Centre of Crop Chemical Control, Department of Agronomy, State Key Lab of Plant Physiology and Biochemistry, China Agricultural University, Beijing 100094, China)

Seedling responses of two cultivars of transgenic Bacillus thuringiensis （bt） cotton （Gossypium hirsutum L.）, 99B and SGK9708-41, to mepiquat chloride （MC） applications (0 and 200 mg·kg-1) were stu

关键词： bt protein bt cotton heat stress gossypol condensed tannin

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：