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Breeding and Characterization of a New Rice Restorer Line Containing bt gene

Rice science 2009年第3期16卷 194-200页

作者： GAO Fang-yuan LU Xian-jun HE Shu-lin CHEN Xiao-juan Lu Dai-hua SUN Shu-xia LI Zhi-hua LIU Guang-chun ZHANG Yi-zheng REN Guang-jun Institute of Crop Sciences Sichuan Academy of Agricultural Sciences Chengdu 610066 China College of Life Sciences Sichuan University Chengdu 610041 China Innovation Center of Rice Regional Technology Ministry of Agriculture Chengdu 610066China Institute of Plant Protection Sichuan Academy of Agricultural Sciences Chengdu 610066 China

bt5198, a new rice restorer line containing bt gene, was developed from the cross and backcross of the elite restorer line Chenghui 177 with bt Minghui 63, a transgenic bt restorer line. The inbred lines were evaluated using PCR amplification, test paper evaluation, insect resistance evaluation in both the laboratory and paddy fields, nursery evaluation of rice blast resistance and pedigree selection of agronomic traits. Larval mortalities on bt5198 and bt Minghui 63 were 100% when rice culms were inoculated with the eggs of the striped stem borer （SSB） in the laboratory. bt5198 was highly resistant against SSB and the yellow stem borer （YSB） under field conditions. The F1 hybrids derived from bt5198 and four cytoplasmic male sterile （CMS） lines were also highly resistant to SSB and YSB and had a significant heterosis. Two-year evaluation of rice blast resistance confirmed that the resistance levels of bt5198 to leaf blast and neck blast were similar to those of Chenghui 177 and significantly better than those of bt Minghui 63. Seed germination ability and pollen yield of bt5198 were similar with Chenghui 177, suggesting that the introduction of the bt gene into the new restorer line had no significant effects on seed vitality or the yield of seed production. To identify the presence of the bt gene, it was effective to combine test paper examination with the evaluation of insect-resistance, both in the laboratory and under field conditions.

关键词： hybrid rice restorer line bt gene insect resistance Magnaporthe oryzae seed germination pollen yield

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The Research of bt and OC gene Cotransformation in Tobacco Chloroplast

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Agricultural Sciences in China 2002年第6期1卷 657-661页

作者： SU Ning, YANG Bo, MENG Kun, LI Yi-nu, SUN Meng, SUN Bing-yao and SHEN Gui-fang(Biotechnology Research Institute, Chinese Academy of Agricultural Sciences , Beijing 100081 , P. R . China) Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 P. R. China

The bt Cry IA (C) chloroplast expression cassette and OC chloroplast expression cassette were constructed. The bt expression cassette contained the 3.5 kb wild type bt Cry I4(C) gene under the control of the strong light-induced psbA promoter and terminator from rice (Oryza saliva . L) chloroplast, the gene: trnH-psbA-trnk from tobacco (Nicotiana tabacum. L) as the homologous fragment. The OC chloroplast expression cassette contained the OC gene under the control of 16S promoter and terminator from tobacco, the tobacco gene: psbA-ORF512 as homologous fragment. The two cassettes both had the aadA gene expression cassette as the selectable marker. Leaves of tobacco were cotransformed with the particle bombardment method. After selection by spectinomycin, the transformants were obtained. The integration of bt and OC gene were confirmed by Southern-blotting analysis, and Western-blotting analysis. Proteinase inhibitor assays showed that the bt and OC gene had expressed. Bioassays showed that the transgenic tobacco had a significant resistance to the larvae of cotton bollworm (helicoverpa zea).

关键词： OC gene bt gene Chloroplast transformation Insect resistance

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Transformation of Cabbage(Brassica oleracea var.capitata)with bt and the Inheritance of Target gene

Transformation of Cabbage(Brassica oleracea var.capitata)wit...

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湖北省遗传学会第七次代表大会暨学术讨论会

作者： Ruohe Yin~(1 2), Hanxia Li~2, Bo Ouyang~1, Yinhua Chen, Yachun Lu, Zhibiao Ye~1 (1 National Key Laboratory of Crop genetic Improvement, 2 College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070)

Cabbage was widely planted in china for its abundant nutrition and special flavor. It has been extensively investigated for plant genetic engineering. bt genes were derived from Bacillus thuringiensis, and their toxin proteins could kill lepidopters specifically but were harmless to higher animal and human being. Thereby, it was of potential to obtain insect-resistance resource by introducing bt gene into cabbage. The hypocotyl segments of cabbage (Brassica oleracea var. capitata cv. Zhonggan-8 and Niuxin) sterile seedlings were used as explants in this experiment. Through

关键词： bt gene transgenic cabbage gertetie analysis Plutella xylostella linne

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Advances in the Identification of genetically Modified Rice with Real-time PCR and Multiplex PCR

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Agricultural Biotechnology 2017年第3期6卷 23-25,29页

作者： Juan QIU Institute of Food Crops Hubei Academy of Agricultural Sciences

In recent years, food security and safety have attracted increasing attention due to the worldwide research and development of genetically modified （GM） rice, and the controversy over the commercialization of GM rice. And the identification of GM rice is of great significance. Therefore, in the present study, the po- tential problems in the identification of GM rice with PCR were analyzed both at a technical level and from a theoretical perspective. In addition, PCR detection on the transgenic elements： promoter, terminator, internal reference gene and target gene was discussed, respectively. The possible solutions were proposed based on the principles of plant virology and genetic engineering.

关键词： genetically modified （GM） rice Qualitative detection Promoter Terminator bt gene Multiplex PCR Real-time PCR

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