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Biosynthesis and Immunological Evaluation of a Dual-Antigen Nanoconjugate Vaccine Against brucella melitensis

Engineering 2023年第10期29卷 95-109页

作者： Jing Huang Yufei Wang Kangfeng Wang Shulei Li Peng Sun Yan Guo Jiankai Liu Ruifu Yang Ming Zeng Chao Pan Hengliang Wang Li Zhu State Key Laboratory of Pathogen and Biosecurity Beijing Institute of BiotechnologyBeijing 100071China Beijing Minhai Biotechnology Co. Ltd.Beijing 102600China State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China The Third Medical Center PLA General HospitalBeijing 100039China College of Life Science Hebei UniversityBaoding 071002China

Brucellosis,caused by brucella,is one of the most common ***,there is still no vaccine for human *** some live attenuated vaccines have been approved for animals,the protection effect is not *** this study,we developed a dual-antigen nanoconjugate vaccine containing both polysaccharide and protein antigens against ***,the antigenic polysaccharide was covalently coupled to the outer membrane protein Omp19 using protein glycan coupling technology,and then it was successfully loaded on a nano-carrier through the SpyTag/SpyCatcher *** confirming the efficient immune activation and safety performance of the dual-antigen nanoconjugate vaccine,the potent serum antibody response against the two antigens and remarkable protective effect in non-lethal and lethal brucella infection models were further demonstrated through different routes of *** results indicated that the dual-antigen nanoconjugate vaccine enhanced both T helper 1 cell(Th1)and Th2 immune responses and protected mice from brucella ***,we found that this protective effect was maintained for at least 18 *** our knowledge,this is the first brucella vaccine bearing diverse antigens,including a protein and polysaccharide,on a single ***,we also present an attractive technology for co-delivery of different types of antigens using a strategy applicable to other vaccines against infectious diseases.

关键词： Protein glycan coupling technology(PGCT) Dual-antigen Nanoconjugate vaccine brucella melitensis

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In vitro assay for the anti-brucella activity of medicinal plants against tetracycline-resistant brucella melitensis

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第7期11卷 506-511页

作者： Hossein MOTAMEDI Esmaeil DARABPOUR Mahnaz GHOLIPOUR Seyyed Mansour SEYYED NEJAD Department of Biology Faculty of ScienceShahid Chamran University

Brucellosis, a zoonosis caused by four species of brucella, has a high morbidity. brucella melitensis is the main causative agent of brucellosis in both human and small ruminants. As an alternative to conventional antibiotics, medicinal plants are valuable resources for new agents against antibiotic-resistant strains. The aim of this study was to investigate the usage of native plants for brucellosis treatment. For this purpose, the anti-brucella activities of ethanolic and methanolic extracts of Salvia sclarea, Oliveria decumbens, Ferulago angulata, Vitex pseudo-negundo, Teucrium polium, Plantago ovata, Cordia myxa, and Crocus sativus were assessed. The activity against a resistant Br. melitensis strain was determined by disc diffusion method at various concentrations from 50-400 mg/ml. Antibiotic discs were also used as a control. Among the evaluated herbs, six plant (Salvia sclarea, Oliveria decumbens, Ferulago angulata, Vitex pseudo-negundo, Teucrium polium, and Crocus sativus) showed anti-brucella activity. Oliveria de- cumbens was chosen as the most effective plant for further studies. A tested isolate exhibited resistance to tetracycline, nafcillin, oxacillin, methicillin, and colistin. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values for Oliveria decumbens against resistant Br. melitensis were the same (5 mg/ml), and for gentamicin they were both 2 mg/ml. Time-kill kinetics for a methanolic extract of Oliveria decumbens was 7 h whereas for an ethanolic extract it was 28 h. Also, Oliveria decumbens extracts showed a synergistic effect in combination with doxycycline and tetracycline. In general, the similar values of MIC and MBC for Oliveria decumbens suggest that these extracts could act as bactericidal agents against Br. melitensis. In addition to Oliveria decumbens, Crocus sativus and Salvia sclarea also had good anti-brucella activity and these should be considered for further study.

关键词： Brucellosis Antibiotic resistance brucella melitensis Medicinal plant Oliveria decumbens

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Epidemiologically characteristics of human brucellosis and antimicrobial susceptibility pattern of brucella melitensis in Hinggan League of the Inner Mongolia Autonomous Region,China

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Infectious Diseases of Poverty 2020年第3期9卷 146-147页

作者： Hai-Tao Yuan Cheng-Ling Wang Li-Na Liu Dan Wang Dan Li Zhen-Jun Li Zhi-Guo Liu Hinggan League Center for Disease Control and Prevention Ulanhot 137400China 不详

Background:Hinggan League is located in the Northeast of the Inner Mongolia Autonomous Region,the historically endemic area of animal and human *** this study,the epidemiological characteristics of human brucellosis were analyzed,and the genotypic profile and antimicrobial susceptibilities of brucella melitensis strains isolated from humans in Hinggan League were ***:The epidemic characteristics were described using case number,constituent ratio,and *** 418 human blood samples were collected and tested by bacteriology,and suspect colonies were isolated and identified by conventional biotyping assays,the VITEK 2.0 microbial identification system,and AMOS(brucella abortus,***,***,and ***)-***,all strains were genotyped using multiple-locus variable-number tandem repeat analysis(MLVA)assays,and the antimicrobial susceptibility pattern of brucella strains against the 10 most commonly used antibiotics was determined by microdilution ***:A total of 22848 cases of human brucellosis were reported from 2004 to 2019,with an annual average incidence of 87.2/*** incidence rates in developed areas of animal husbandry(Horqin Youyi Qianqi[161.2/100000]and Horqin Youyi Zhongqi[112.1/100000])were significantly higher than those in forest areas(Arxan[19.2/100000])(χ2=32.561,P<0.001).In addition,peak morbidity occurred during May–August,accounting for 72.6%(16582/22848)of *** highest number of cases occurred in the 40+age group,accounting for 44.4%(10137/22484)of cases,and morbidity in males was significantly higher than that in females in all age groups(χ2=299.97,P<0.001),the most common occupation was farmers.A total of 54 *** strains were divided into 37 genotypes(GT1–37)with 80–100%genetic *** 25 strains were sensitive to seven tested antibiotics,phenotypic resistance to cotrimoxazole and azithromycin was observed in 5(20%)and 25(100%)of the isolates,***:Hu

关键词： Brucellosis Epidemiology characteristic brucella melitensis Genotyping Antimicrobial susceptibility Hinggan league Inner Mongolia Autonomous Region

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Identification of brucella melitensis and Molecular Cloning of Its BP26 Gene into p ET24a(+) Vector

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Animal Husbandry and Feed Science 2015年第6期7卷 358-363页

作者： Feng Ying Wang Yu Zhao Shihua Gao Wa Zhan Shubai Chen Wei Veterinary Research Institute of Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences Medicinal Chemistry and Pharmacology Institute Inner Mongolia University for the Nationalities

brucella spp. are pathogenic to humans and domestic animal. Nowadays,there is no effective vaccine and control strategy in China. So,it is necessary to research effective vaccines for prevention and treatment of this disease. In order to deal with these,we isolated and identified the type of brucella in Darhan Muminggan Joint Banner and Siziwang Banner of Inner Mongolia. Totally 26 samples of sheep blood which were positive in serological test,one sample of spleen from aborted and one sample of secretion from birth canal were isolated,and the 16 S r DNA genes of positive samples were sequenced. Phylogenetic analysis proved that there were four isolates similar to B. melitensis. As an important diagnostic antigens of B. melitensis,the BP26 gene was amplified. The BP26 gene was cloned into vector p ET24a( +) and conducted sequence analysis. The BP26 gene was 900 bp,with an open reading frame of 753 bp. The homology of BP26 gene with the vaccine strain M5 was 100%,and that with S2 and A19 vaccine strains was 99. 99%. These finding supported the development of BP26-based specific serodiagnostic test and vaccine for B. melitensis in China.

关键词： brucella melitensis Protein BP26 Vector construction

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A retrospective survey of brucella melitensis human infection in Hainan Province,China

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Biosafety and Health 2021年第3期3卷 131-135页

作者： Shuyi Ma Xuming Wang Miao Wang Zhiguo Liu Zhenjun Li School of Medical Technology Baotou Medical CollegeBaotou 014040China Hainan Provincial People's Hospital Haikou 570311China Ulanqab Central for Endemic Disease Control and Prevention Jining 012000China State Key Laboratory for Infectious Disease Control and Prevention National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China.

In China,brucellosis has spread from the northern provinces to the southern coastal area,and brucella melitensis has become the overwhelmingly dominant species *** human Brucellosis cases have been reported in many parts of Hainan Province,investigations into the source of infection have been *** this case,we have used the techniques of bacteriology,genome sequencing,and WGS-SNP to investigate the source of infection on a patient with a traveling history between Hainan and Inner Mongolia autonomous *** collected a 5mL blood sample under sterile conditions from this suspected case of Brucellosis infection and incubated it in a culture bottle.A week later,brucella colonies were purified and identified under the standard bacteriology procedures to show that the strain appeared as *** ***,a draft genome sequencing of this strain was ***-SNP comparison analysis of Chinese *** selected(n=36)fromGenBank revealed that the strain in this studywas more similar to the strains isolated from China's northern parts,especially those from the Inner Mongolia Autonomous *** data indicated that the source of infection of the patient was in the Inner Mongolia Autonomous Region,but the actual location where this patient became infected was *** suggest that travelers from the southern part of China be cautious when visiting the traditional endemic areas of Brucellosis.

关键词： brucella melitensis Biotyping Draft genome sequence SNP

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Unusual causes of intrahepatic cholestatic liver disease

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World Journal of Gastroenterology 2007年第12期13卷 1879-1882页

作者： Elias E Mazokopakis John A Papadakis Diamantis P Kofteridis Department of Internal Medicine University Hospital of Heraklion Crete Greece Department of Internal Medicine NavalHospital of Crete Greece Department of Internal Medicine University Hospital of Heraklion Crete Greece

We report five cases with unusual causes of intrahepatic cholestasis, including consumption of Teucrium polium （family Lamiaceae） in the form of tea, Stauffer＇s syndrome, treatment with tamoxifen citrate for breast cancer, infection with Coxiella Burnetii （acute Q fever）, and infection with brucella rnelitensis （acute brucellosis）.

关键词： brucella melitensis Coxiella Burnetii Cholestasis Hepatitis Stauffer's syndrome Tamoxifen Teucrium polium

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