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中国畜牧兽医学会兽医公共卫生学分会第六次学术研讨会

作者： Jing Qian Zhaoyang Bu Xulong Lang Guangmou Yan Yanling Yang Xiuran Wang Xinglong Wang Key Laboratory of Jilin Province for Zoonosis Prevention and Control Institute of Military Veterinary MedicineAcademy of Military Medical Sciences Laboratory of Infectious Diseases College of Veterinary MedicineJilin University Key Laboratory of Zoonosis Research Ministry of EducationJilin University Institute of Special Wild Animal & Plant Science Chinese Academy of Agricultural Sciences School of Life Science Jilin Agricultural University

Canine Brucellosis,caused by brucella canis,is a persistent infectious reproductive disease in *** absence of effective treatment to the intracellular pathogen and the irreversible consequence of infection makes the need of a specific vaccine *** ghosts（BGs） are the empty envelopes of bacteria with no genome content inside,which emerge as a proper vaccine candidate due to its intact outer *** is generally derived from a genetically engineered strain,through the expression of Bacteriophage phiX174 lysis E gene upon *** this study,we combined the homologous recombination（HR） and bacterial ghost technologies,generating a genetically stable *** ghost strain which bears no drug resistance *** the ghost strain grows to OD600 of 0.6,100% inactivation can be achieved under 42°C in 60 *** resultant BGs showed guaranteed safety and comparable immunogenicity to a live *** bacterial B0419 protein was depleted during HR process,which is subsequently proved to work as a molecular tag in distinguishing natural infection and BGs immunization through ***,the BGs also conferred protection against *** RM6/66 and *** 16 ***,the application of current BGs as a vaccine candidate and the corresponding serological diagnostic approach may provide better *** prevention strategy.

关键词： brucella canis bacterial ghost safety immunogenicity protective efficacy differential diagnosis

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GI_(FeGSH):A New Genomic Island Might Explain the Differences in brucella Virulence

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Open Journal of Animal Sciences 2017年第2期7卷 141-148页

作者： Tara Wahab Astrid Skarp Viveca Baverud Rene Kaden Swedish Forum for Biopreparedness Diagnostics(FBD) UmeaUppsala and SolnaSweden Public Health Agency of Sweden SolnaSweden Department of Medical Sciences Uppsala UniversityUppsalaSweden National Veterinary Institute UppsalaSweden

An imported dog was confirmed to be positive with canine brucellosis in Sweden in 2010. The whole genome of brucella canis SVA10 was subjected to phage analysis (WGS-PA) and was assigned to the Asian B. canis cluster. Further analysis indicated that the genome of B. canis SVA10 is smaller compared to genomes of the same species. A 35,781 bp genomic island (GI) was found to be absent in strain SVA10 which was detected by read mapping the paired reads to the genome of B. canis ATCC 23,365T. The lacking genes of genomic island GIFeGSH are mainly coding for iron uptake enzymes and parts of the glutathione pathway. A screening of all available whole genome sequences of brucella strains confirmed that GIFeGSH is also missing in four more strains of B. canis but present in several strains of B. abortus, B. melitensis, B. suis, B. ovis, B. microti, B. pinnipedialis, and B. ceti. Parts of the GI were present, but scattered in two other B. canis strains. The aim of this study was to find differences in the genomes of brucella which might explain former described differences in virulence. The analysis was extended to all available brucella genomes after the detection of a genomic island in strain SVA10.

关键词： Zoonoses Brucellosis brucella canis Genomic Island

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