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brucea javanica oil inhibits proliferation of hepatocellular carcinoma cells and induces apoptosis via the PI3K/AKT pathway

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Traditional Medicine Research 2021年第2期6卷 44-55页

作者： Yan-Peng Du Zhan Ye Zhao-Jun Zheng You-Dong Li Jing Chen Farah Zaaboul Yong-Jiang Xu Yuan-Fa Liu School of Food Science and Technology Jiangnan UniversityWuxi 214122China State Key Laboratory of Food Science and Technology National Engineering Laboratory for Cereal Fermentation TechnologyNational Engineering Research Center for Functional FoodJiangnan UniversityWuxi 214122China

Background:brucea javanica oil(BJO),distributed primarily in Southeast Asia,has long been utilized as a therapeutic agent for treating ***,its anticancer mechanisms are not clearly *** objective of this study was to examine the mechanisms underlying its treatment of hepatocellular carcinoma ***:CCK8 assay was used to evaluate cell ***33342 staining and flow cytometry analyses were used to examine ***-Tracker Red CMXRos kit was used to measure the membrane potential of *** assay kit was used to evaluate ATP *** blots were used to assess the presence of AKT,adenosine monophosphate-activated protein kinase,Caspase3,Caspase9,Bax,and ***:BJO inhibited the proliferation of hepatocellular carcinoma cells HepG2 in a time-and dose-dependent *** induced apoptosis,with the percentage of cells treated with 50–150μg/mL BJO increasing from 8.01%to 28.02%in a concentration-dependent manner(P<0.05,when 50μg/mL of BJO group compared with the control group;P<0.001,when 100 or 150μg/mL of BJO group compared with the control group).After exposed to BJO,the expression of C-caspase3,C-caspase9 and Bax upregulated while that of Bcl-2 *** suppressed the PI3K/AKT pathway and promoted phosphorylation of adenosine monophosphate-activated protein kinase,while repressing the phosphorylation of mechanistic target of *** with treatment by BJO alone,the PI3K/AKT agonist 740Y-P increased the survival rate of HepG2 cells(P<0.01)and attenuated the inhibitory effect of BJO on cell apoptosis(P<0.05).Conclusion:BJO is capable of inhibiting proliferation of HepG2 cells and inducing apoptosis via the PI3K/AKT pathway.

关键词： brucea javanica oil Hepatocellular carcinoma HepG2 cell Cell proliferation Cell apoptosis PI3K/AKT

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Formulation development and evaluation of gastroretentive floating beads with brucea javanica oil using ionotropic gelation technology

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Chinese Journal of Natural Medicines 2018年第4期16卷 293-301页

作者： ZHANG Yue ZHANG Xi-Tong ZHANG Qi WANG Bing ZHANG Tong Experiment Center for Teaching and Learning Shanghai University of Traditional Chinese Medicine School of Pharmacy Shanghai University of Traditional Chinese Medicine

In the present study, a gastric retention floating system for brucea javanica oil, composed of alginate and carrageenan, was prepared using ionotropic gelation. Parameters for floatability, drug load, encapsulation efficiency, bead morphology, in vitro release, and in vivo gastric retention were evaluated. The optimized formulation via Box–Behnken design consisted of 1.7% alginate(W/V), 1.02% carrageenan(W/V), 1.4% CaCO_3(W/V), and a gelling bath of pH 0.8. The alginate–carrageenan–brucea javanica oil beads had a porous structure and exhibited up to 24 h of in vitro floatability with a load capacity of 45%–55% and an encapsulation efficiency of 70%–80%. A 6-h sustained release was observed in vitro. The beads had a prolonged gastric retention(> 60% at 6 h) in fasted rats, compared to non-floating beads(15% at 6 h), as measured by gamma scintigraphy with single-photon emission tomography/computed tomography(SPET/CT). In conclusion, the alginate–carrageenan–brucea javanica oil system showed enhanced oil encapsulation efficiency, excellent floating and gastric retention abilities, and a favorable release behavior.

关键词： brucea javanica oil Simaroubaceae Alginate–carrageenan beads Gastric retention Box–Behnken design SPET/CT

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Preparation and evaluation of paclitaxel and brucea javanica oil core-matched nanoemulsions to treat cancer in vitro and in vivo

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Chinese Herbal Medicines 2018年第3期10卷 310-317页

作者： Shu-qing Cao Kuan-yun Zhang Xia Yan Yan Ma School of Pharmacy Guangzhou University of Chinese Medicine College of Medical Jiaying University

Objective: Developed the core-matched nanoemulsions(CMNEs) to co-delivery paclitaxel-oleic acid(PTXOA) prodrug and brucea javanica oil(BJO) for increasing the antitumor ***: Antitumor effects and mechanism of PTX-OA/BJO CMNEs that the combination therapy which based on core-matched technology(CMT) were evaluated in vitro and in ***: The PTX-OA/BJO CMNEs were of nanoscale particle size(108.7 ± 2.3) nm and with entrapment efficiency of >95%. The PTX-OA/BJO CMNEs displayed concentration and time-dependent cytotoxicity against HepG-2 cells and increased G2/M phase block. More importantly, a significant reduction of the tumor volume with no obvious toxicity was observed in nude mice model following administration of PTX-OA/BJO CMNEs compared with the control treated with normal saline(P < 0.05), which suggested the excellent efficacy in vivo. It was further found that the enhanced effectiveness of PTX-OA/BJO CMNEs were associated with the ability of inducing apoptosis of the tumor cells, as well as obviously inhibiting tumor cell proliferation and the activity of TOPOⅡ.Conclusion: Co-encapsulation of two drugs with different mechanisms allows simultaneous interruption of diverse anticancer pathways, resulting in increased therapeutic response and lower toxicity.

关键词： brucea javanica oil cancer combination therapy core-matched nanoemulsion paclitaxel

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