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bromelain and Cardiovascular Risk Factors in Diabetes:An Exploratory Randomized,Placebo Controlled,Double Blind Clinical Trial

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Chinese Journal of Integrative Medicine 2016年第10期22卷 728-737页

作者：黎七妹倪青廖星高怀林 Nicola Robinson School of Health and Social Care London South Bank University Department of Endocrinology Guang'anmen Hospital China Academy of Chinese Medical Sciences Institute of Basic Research in Clinical Medicine China Academy of Chinese Medical Sciences Department of Endocrinology Yiling Hospital Affiliated to Hebei Medical University

Objective： To assess whether the dietary supplement （bromelain） has the potential to reduce plasma fibrinogen and other cardiovascular disease （CVD） risk factors in patients with diabetes. Methods： This randomized placebo controlled, double blind, parallel design, efficacy study was carried out in China and investigated the effect of 12 weeks of bromelain （1,050 mg/day） on plasma fibrinogen, This randomized controlled trial （RCT） recruited 68 Chinese diabetic patients [32 males and 36 females; Han origin, mean age of 61.26 years （standard deviation （SD）, 12.62 years）] with at least one CVD risk factor. Patients were randomized into either bromelain or placebo group. While bromelain group received bromelain capsule, the placebo group received placebo capsule which consisted inert ingredient and has no treatment effect. Subjects were required to take 1,050 mg （3× 350 mg） of either bromelain or starch-tiffed placebo capsules, two to be taken （2 × 350 mg） after breakfast and another （350 mg） after dinner, daily for 12 weeks. Plasma fibrinogen, CVD risk factors and anthropometric indicators were determined at baseline and at 12 weeks. Results： The change in the flbrinogen level in the bromelain group at the end of the study showed a mean reduction of 0.13 g/L （standard deviation （SD） 0.86g/L） compared with the mean reduction of 0.36 g/L （SD 0.96 g/L） for the placebo group. However, there was no significant difference in the mean change in fibrinogen between the placebo and bromelain groups （mean difference=0.23g/L （SD 0.22 g/L）, P=0.291）. Similady, the difference in mean change in other CVD risk factors （blood lipids, blood pressure）, blood glucose, C-reactive protein and anthropometric measures between the bromelain and placebo groups was also not statistically significant. Statistical differences in fibrinogen between bromelain and placebo groups before the trial despite randomization may have influenced the results of this study. Conclusion： This RCT failed to show a

关键词： cardiovascular disease fibrinogen diabetes bromelain randomized control trial

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Invulnerability of bromelain against oxidative degeneration and cholinergic deficits imposed by dichlorvos in mice brains

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Frontiers in Biology 2018年第1期13卷 56-62页

作者： Bharti Chaudhary Sonam Agarwal Renu Bist Department of Bioscience and Biotechnology Banasthali University Banasthali Rajasthan India

BACKGROUND： The present study elucidates the protective potential ofbromelain against dichlorvos intoxication in mice brains. Dichlorvos induces the oxidative stress by disproportionating the balance between free radicals generation and their scavenging in neurons which leads to neuronal degeneration. METHODS： In this study, mice were divided into four groups- group I （control）, group II （dichlorvos treated）, group III （bromelain treated） and group IV （exposed to both bromelain and dichlorvos both）. RESULTS： Dichlorvos treatment increased the levels ofthiobarbituric acid reactive substances （TBARS） and protein carbonyl content （PCC） which indicate the increased oxidative stress. Meanwhile, brain endogenous antioxidants and cholinesterases level was decreased after dichlorvos exposure. Levels of TBARS and PCC decreased whereas cholinesterases level was recorded to be elevated after bromelain exposure. CONCLUSION： bromelain offered neuroprotection by decreasing oxidative stress and augmenting cholinesterases in mice brains. This study highlights the invulnerability of bromelain against oxidative and cholinergic deficits in mice brains.

关键词： oxidative stress dichlorvos bromelain neuroprotection neurotransmitter

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Kinetic studies on recombinant stem bromelain

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Advances in Enzyme Research 2013年第3期1卷 52-60页

作者： Muntari Bala Maizirwan Mel Mohamed Saedi Jami Azura Amid Hamzah Mohd Salleh Bioprocess and Molecular Engineering Research Unit Department of Biotechnology Engineering International Islamic University Malaysia Kuala Lumpur Malaysia International Institute for Halal Research & Training (INHART) International Islamic University Malaysia Kuala Lumpur Malaysia

Stem bromelain is a plant thiol protease with several industrial and therapeutic applications. This current work presents kinetic studies of recombinant bromelain (recBM) expressed in Escherichia coli BL21-AI on foursynthetic substrates, N-α-carbobenzoxy-L-alanyl-p-nitrophenylester (ZANPE), N-α-carbobenzoxy-L-arginyl-L-ar-ginine-p-nitroanilide (ZAANA), N-α-carbobenzo-xy-L-phenylalanyl-L-valyl-L-arginine-p-nitroanili-de (ZPVANA) and L-pyroglutamyl-L-phenylalanyl-L-leucine-p-nitroanilide (PFLNA). Hydrolytic activities of recBM at various pH and temperature conditions were compared to that of commercial bromelain (cBM). Both enzymes demonstrated high activities at 45o C and pH 5 - 8 for recBM and pH 6 - 8 for cBM. recBM showed marginally lower Kmand slightly higher kcat/Kmfor ZAANA, ZANPE and ZPVANA in comparison to *** Epoxysuccinyl-L-leucylamido {4- guanidino}butane (E-64) severely affected recBM and cBM hydrolysis of the synthetic substrates by competitive inhibition with Kivalues of 3.6 - 5.1 μM and 5.5 - 6.9 μM for recBM and cBM, respectively. The evaluated properties of recBM including temperature and pH optima, substrate specificity and sensitivity to inhibitors or activators, satisfy the requisites required for food industries.

关键词： Protease Recombinant bromelain Substrates Inhibitors Activators

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Application of bromelain Extract for Muscle Foods Tenderization

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Food and Nutrition Sciences 2011年第5期2卷 393-401页

作者： Sunantha Ketnawa Saroat Rawdkuen Food Technology Program School of Agro-Industry Mae Fah Luang University Muang Chiang Rai Thailand.

This study investigates the tenderizing effects of bromelain extract (BE) obtained from the top phase of an aqueous two-phase system comprising of 18% PEG 6000–17% MgSO4 in muscle foods. Uniform-sized chunks of beef, chicken, and squid were marinated with different concentrations of BE (0, 3, 7%, and 20%, w/w) for 1 h at room temperature. The physico-chemical and quality characteristics of the marinated samples were determined. Decreases in pH and moisture content were observed in the samples BE treated, but the TCA-soluble peptides content significantly increased (p bromelain extract derived from the two-phase extraction of a pineapple peel could be used as an effective meat tenderizer.

关键词： bromelain Pineapple Peel Aqueous Two Phase System Muscle Food Meat Tenderization

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Optimisation of beef tenderisation treated with bromelain using response surface methodology (RSM)

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Agricultural Sciences 2013年第5期4卷 65-72页

作者： S. Zainal K. Z. Nadzirah A. Noriham I. Normah Department of Food Science and Technology Faculty of Applied Sciences Universiti Teknologi MARA Shah Alam Selangor Malaysia

The purpose of this study is to determine the optimum condition for the tenderization of beef by bromelain using Response Surface Methodology (RSM). Initially, bromelain powder was produced from pineapple crown of variety N36. Production of the bromelain powder involves several process steps such as extraction, purification, desalting and freeze drying. The cube size beef of round part was treated with bromelain at different pHs of beef, immersion temperatures, bromelain solution concentrations, and immersion times according to the experimental design which was recommended by RSM of MINITAB software version 15. Beef tenderness was then measured by Texture Analyser. The MINITAB software Version 15 was used to optimise the tenderisation of beef by bromelain. The determination coefficient R2 was 99.97% meaning that the experimental data were acceptable. It was found that beef could be optimize tenderised 89.907% at the optimum condition at pH of beef of 5.4, immersion temperature of60℃, bromelain solution concentration of 0.1682% and immersion time of 10 minutes. The verification value of beef ten-derisation at the feasible optimum condition which was determined by experiment was 89.571%. Since the difference between the veri- fication and predicted values was less than 5%, therefore, the optimum condition for the tenderisation of beef predicted by MINITAB software Version 15 could be accepted.

关键词： Beef bromelain Tenderisation Response Surface Methodology (RSM)

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bromelain对HL-60细胞的诱导分化作用

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癌症 1997年第6期16卷 401-404页

作者：颜青杨善民朱林彬王重钦周红厦门大学抗癌研究中心厦门大学生物系

目的：为获得对ＨＬ－６０细胞具有高效低毒作用的天然诱导分化剂而进行的研究。方法：以菠萝酶Ｂｒｏｍｅｌａｉｎ为药物，对人前髓细胞性白血病细胞株ＨＬ－６０进行作用，通过不同浓度及不同作用时间的药物作用，统计ＨＬ－６０细胞... 详细信息

目的：为获得对ＨＬ－６０细胞具有高效低毒作用的天然诱导分化剂而进行的研究。方法：以菠萝酶Ｂｒｏｍｅｌａｉｎ为药物，对人前髓细胞性白血病细胞株ＨＬ－６０进行作用，通过不同浓度及不同作用时间的药物作用，统计ＨＬ－６０细胞的增殖率、生化变化及形态学的变化。结果：发现Ｂｒｏｍｅｌａｉｎ在高浓度下（８μｇ／ｍｌ）作用４天即可诱导ＨＬ－６０细胞发生显著的诱导分化，其ＮＢＴ阳性率及形态学指标均达到６０％以上，电镜下核异染色质浓聚，出现特异性颗粒。结论：Ｂｒｏｍｅｌａｉｎ对ＨＬ－６０细胞具有明显的诱导分化作用，但其作用机理仍需进一步研究。

关键词： bromelain 诱导分化蛋白水解酶药物疗法

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A Study of the Enzymatic Hydrolysis of Fish Frames Using Model Systems

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Food and Nutrition Sciences 2011年第6期2卷 575-585页

作者： Aristotelis T. Himonides Anthony K. D. Taylor Anne J. Morris Technological Educational Institute Thessaloniki Greece

A model system was employed to study the operating conditions and primary parameters of enzymic hydrolysis of cod proteins. Pancreatin, papain, and bromelain were used to hydrolyse minced cod fillets under controlled conditions and with the rate of hydrolysis being continually monitored via both the pH-stat and TNBS method. The two methods were compared and evaluated. The rate of protein solubilisation was plotted against the degree of hydrolysis (DH). Dry fish protein hydrolysate (FPH) powders having short, medium and high degrees of hydrolysis (DH of approximately 8%, 11% and 16% respectively) were produced and analysed for their molecular weight distribution, using size exclusion chromatography. Almost complete protein solubilisation (75 g soluble protein per kg hydrolysis solution) could be achieved within an hour, at 40oC, at 1% enzyme/substrate ratio (w/w) with papain and bromelain. The pH-stat was found capable of continuously following the rate of hydrolysis but only at low DH. The TNBS could be accurately used even at high DH to estimate the percentage of the peptide bonds cleaved, but required chemical analysis of withdrawn samples.

关键词： Enzymatic Hydrolysis Papain bromelain Pancreatin Fish Frames Cod Fish Protein Hydrolysates

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An updated review of pineapple and its bioactive compounds in breast cancer

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Cancer Advances 2023年第5期6卷 1-5页

作者： Virender Kumar Davinder Kumar Vandana Garg Harish Dureja Department of Pharmaceutical Sciences Maharshi Dayanand UniversityRohtakHaryana 124001India.

Women are more likely than men to develop cancer of the *** breast cancer drugs are highly toxic,and treatment can cause side *** is imperative to find safe alternative medicines in the pursuit of a cure for breast *** extract of pineapple contains cysteine proteases known as *** general,pineapples are regarded as safe *** the fruit,stem,and of pineapples,bromelain is produced by multiple *** well as reducing the growth of tumors locally,bromelain severely impaired the cytotoxicity of monocytes in the immune system in the fight against ***,we investigated pineapple’s possible mechanisms of action and its bioactive compounds in breast cancer.

关键词： Ananas comosus proteinases bromelain pineapple plant phototherapeutic applications

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