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Field Trapping Technology of Sex Attractants against Major Pests of broccoli

Plant Diseases and Pests 2020年第1期11卷 22-29页

作者： Liu Weiming Wang Enguo Liu Yenan He Xianbiao Taizhou Vocational College of Science&Technology Taizhou 318020China Linhai Agricultural and Rural Bureau Linkai 317000China Taizhou Academy of Agricultural Sciences Linhai 317000China

[Objective]The paper was to explore the comprehensive sexual attraction control technology of main pests in broccoli,and to improve the green control technology of main pests in broccoli.[Method]The comprehensive control technology of sex attractants against major pests in broccoli was studied by using special sex attractants,lures and traps.[Result]The mixed quantities of Spodoptera litura,Spodoptera exigua and Plutella xylostella were fluctuated in a"W"shape in broccoli growing season in coastal area of Taizhou,central Zhejiang province,including seedling growth period(July to August),rosette and bud growth period(late September to early November),late harvest period of late-ripening broccoli(after early march).*** and *** occurred severely at seedling stage;***,*** and *** occurred simultaneously at rosette and bud growth period;*** damaged florets of lateral branches in late harvest *** trapping effects of combination A and E against ***,*** and *** were the best;more than 1260 individuals of ***,*** and *** were daily trapped per hectare,and the occurrence and damage of the three insect pests could be basically *** the sex attract produced in Ningbo as an example,the combination of *** S type∶*** S type∶*** blue type=1∶1∶4 distributed in five stars of clubs(space 10-20 cm)showed good comprehensive trapping effect in large area demonstration;traps were hung at the height of 0.5 m(0.2 m for ***),and lures were replaced once every 30-40 d.[Conclusion]Using special sex attractants,the comprehensive effects on ***,*** and *** were determined at rosette and bud growth period of broccoli through combination modes,and supporting application technology were put forward,making great progress in exploring and improving sex attractant combination against insect pests.

关键词： broccoli Sex attractant Spodoptera litura Spodoptera exigua Plutella xylostella

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Effects of broccoli extract and various essential oils on intestinal and faecal microflora and on xenobiotic enzymes and the antioxidant system of piglets

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Open Journal of Animal Sciences 2012年第2期2卷 78-98页

作者： Kristin Mueller Nicole M. Blum Holger Kluge Rolf Bauerfeind Julia Froehlich Anneluise Mader Karola R. Wendler Andreas S. Mueller Delacon Biotechnik Ges.m.b.H. Steyregg Austria Institute of Agricultural and Nutritional Sciences Preventive Nutrition Group Martin Luther University of Halle-Wittenberg Halle Germany Institute of Animal Nutrition Free University of Berlin Berlin Germany Institute of Veterinary Hygiene and Infectious Diseases of Animals Justus Liebig University Giessen Giessen Germany

Objective: Since the ban of antibiotics as growth promoting feed additives in the EU in 2006 research in alternatives has gained importance. Phytogenic feed additives represent a heterogenous class of different plant derived substances that are discussed to improve the health of farm animals by direct and indirect antioxidant effects and by influencing microbial eubiosis in the gastrointestinal tract. Consequently our study aimed to investigate the influence of broccoli extract and the essential oils of tur- meric, oregano, thyme and rosemary, as selected individual additives, on intestinal and faecal microflora, on xenobiotic enzymes, and on the antioxidant system of piglets. Methods: 48 four weeks old male weaned piglets were assigned to 6 groups of 8. The piglets were housed individually in stainless steel pens with slatted floor. The control group (Con) was fed a diet without an additive for 4 weeks. The diet of group BE contained 0.15 g/kg sulforaphane in form of a broccoli extract. 535, 282, 373 and 476 mg/kg of the essential oils of turmeric (Cuo), oregano (Oo), thyme (To) and rosemary (Ro) were added to the diets of the remaining 4 groups to stan-dardise supplementation to 150 mg/kg of the oils’ key terpene compounds ar-turmerone, carvacrol, thymol and 1,8-cineole. The composition of bacterial microflora was examined by cultivating samples of jejeunal and colonic mucosa and of faeces under specific conditions. The mRNA expression of xenobiotic and antioxidant enzymes was determined by reversing transcrip- tase real time detection PCR (RT-PCR). Total antioxidant status was assayed using the Trolox Equivalent Antioxidant Capacity (TEAC), and lipid peroxidation was determined by measuring thiobarbioturic acid reactive substances (TBA- RS). Results: Compared to Con piglets all additives positively influenced weight gain and feed conversion in week 1. Over the whole trial period no significant differences in performance parameters existed between the experimental

关键词： Pigs Phytogenic Feed Additives broccoli Extract Essential Oils Xenobiotic Enzymes Antioxidant System

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Identification of Resistance to Blackrot in broccoli

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Journal of Northeast Agricultural University(English Edition) 2002年第2期9卷 106-110页

作者： WANG Chao,ZHANG Gui ling,WU Shi chang (Department of Horticulture,Northeast Agricultural University,Harbin,Heilongjiang,150030,PRC) Department of Horticulture Northeast Agricultural UniversityHarbinHeilongjiang150030PRC

The screening for Black rot(Xanthomonas campestris ***)resistance in broccoli (Brassica oleracea *** Planch) lines plays an important role in breeding for Black rot resistance and is of great value to practical utilization. The broccoli in bred line"91-9318-4"was used as the experimental material in the trial.X 0-2 strain of Xanthomonas campestris *** was and purified from the infected broccoli plants in the *** spraying method was adopted in the *** trial was designed as the randomized blocks of two factors with three *** A was the foliar age and factor B was the concetrations of *** A had three levels A 1,A 2 and A 3 (the 2nd 4th and 6th true leaf stage),factor B had three levels B 1,B 2 and B 3(10 6 counts/mL,10 7 counts/mL and 10 8 counts/mL). Results of this experiment showed that the best method for screening Black rot resistance in broccoli is ①foliar age:the 4th true leaf stage;②concetration of inoculums;10 7 counts/mL;③temperature at 20～30℃ (from the end of June to the end of July at he local climate condition.) 26 breeding materials of broccoli were identified for resistance to Black rot by means of the acquired *** resistant breeding material was obtained,the identifying results showed that this screening method is practical.

关键词： broccoli black rot spray resistance

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The Effect of Exogenous Sodium Nitroprusside Treatment on broccoli Quality During the Storage Process

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Chinese Food Science 2012年第3期1卷 13-15,19页

作者： WANG Qing SHI Jun-yan YANG Na GAO Li-pu PANG Jie Vegetable Research Center Beijing Academy of Agriculture and Forestry Sciences College of Food Science Fujian Agriculture and Forestry University

[Objective] The aim was to provide certain theoretical basis for the protection of broccoli. [Method] Exogenous sodium nitroprusside was used to in the experiment. Under the condition of 20 ℃ and RH 95%, the optimal SNP concentration of broccoli was determined. [Result] The exogenous SNP could effectively maintain the quality of broccoli, inhabited the yellowing of broccoli and the increasing of cell membrane permeability, and maintain the soluble solids content, decreased the degradation rate of chlorophyll and delayed the loss of VC. [Conclusion] Exogenous sodium nitroprusside can effectively maintain the quality of broccoli to keep it fresh.

关键词： broccoli Sodium nitroprusside(SNP) Storage Quality China

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Effect of Different Growth State of broccoli (Brassica oleracea var. italica) on Low Temperature Induction

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Journal of Northeast Agricultural University(English Edition) 2005年第1期12卷 20-23页

作者： JIANGXin-mei LIYue-fang YUXi-hong HorticultureCollege NortheastAgriculturalUniversityHarbinHeilongjiang150030PRC

In order to study the responding of different growth state on low temperature in broccoli (Brassica oleracea var. italica), we took prematurity broccoli hybrid as the objects. It was found that growth state was varied under different sowing time , moreover, stem became wider with the increasing of light density at the same leaf age. Seedling age of responding to low temperature vernalization only when they grew five leaves above and with over 3.03 ±0.07 centimeter stalk width in 'Qingfeng broccoli 103'. The older leaf age was and the stronger plant was, the more sensitive and the shorter demanded duration time on low temperature was, and the shorter time required when the plants entered into critical period of floral bud differentiation.

关键词： broccoli low temperature leaf age floral bud differentiation

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Occurrence and Damage Regularity of Spodoptera litura in broccoli

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Plant Diseases and Pests 2021年第4期12卷 5-8页

作者： Weiming Liu Enguo Wang Yenan Liu Taizhou Vocational College of Science&Technology Taizhou 318020China Linked Plant Protection Station Linked 317000China Taizhou Academy of Agricultural Sciences Linhai 317000China

[Objective] The paper was to explore the occurrence and damage regularity of Spodoptera litura in broccoli,and to reveal the annual movement regularity and seasonal fluctuation regularity of *** population.[Method] The monitoring data of sexual attractants at fixed points for 11 consecutive years were summarized and analyzed.[Result] The large occurrence frequency of *** in Taizhou was 36.4%;the annual movement showed a downward-opening parabolic form,and the movement trajectory was N =-18.416 t^(2)+182.51 t +179.71,t ={1,2,3,…,n}(n =11;r =0.691 7*).The seasonal fluctuation showed a trend of peak oscillation attenuation curve,and the total moth quantity gradually decreased from sowing to heading *** functional model of average daily quantity was m=0.000 2 d^(2)-0.067 5 d+6.797 3,d={1,2,3,…,n}(n =186,r=0.955 2**).Air temperature was the main external factor affecting the seasonal variation of quantity,and there was a significant correlation be-tween the number of ten-day moth trapping quantity and ten-day average temperature:M =0.243 T^(2)-6.935 1 T +46.681(n =108,r =0.560 2**)or M=2.734 4 T-35.446(n=108,r=0.500 3**).Therefore,the moth quantity in the field increased with the increase of ten-day average temperature,and the period from July to September with high temperature and less rainfall was the peak occurrence period of ***.[Conclusion] These models can be used to improve the prediction and early warning levels of *** in broccoli,and has good guiding significance for decision-making and prevention.

关键词： broccoli Spodoptera litura Seasonal fluctuation Impact factor

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Analysis on Composition and Content of Glucosinolates in broccoli Flowers and Leaves

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Asian Agricultural Research 2017年第4期9卷 81-84页

作者： Guangmin LIU Yue MA Yunhua DING Liping HU Xuezhi ZHAO Hongju HE Beijing Vegetable Research Center Beijing Academy of Agriculture and Forestry Sciences/Beijing Key Laboratory of Fruits and Vegetables Preservation and Processing/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China)Ministry of Agriculture/Key Laboratory of Urban Agriculture(North)Ministry of Agriculture

Glucosinolate composition and content were evaluated in flowers and leaves of 12 different broccoli varieties. The results indicated that there were 9 glucosinolates in broccoli,namely Glucoiberin(IBE); Progoitrin(PRO);Sinigrin(SIN);Glucoraphanin(RAA);Gluconapin(NAP);4-Hydroxyglucobrassicin(4OH);Glucobrassicin(GBC);4-Methoxyglucobrassicin(4ME);Neoglucobrassicin(NEO).Total glucosinolate content in flowers was 1-5 times higher than in leaves. The predominant glucosinolate in broccoli was glucoraphanin.

关键词： broccoli Flowers Leaves Glucosinolates

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Effects of Humic Acid and PASP Compound Fertilizer Application on Growth,Quality and Yield of broccoli

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Agricultural Science & Technology 2016年第3期17卷 535-538页

作者：代明陈庆飞胡兆平李新柱张金涛王建康冯海娟金正大生态工程集团股份有限公司国家缓控释肥工程技术研究中心山东省新型肥料创制与养分资源高效利用重点实验室山东临沂276700

[Objective] This study was conducted to analyze the effects of humic acid and PASP on the growth of broccoli to provide a basis for fertilizer application.[Method] A field experiment was carried out to investigate broccoli growth, yield and quality by using humic acids and PASP as fertilizer synergists in the mixture with compound fertilizer. [Results] The treatment of 5‰ humic acid ＋ 3‰ PASP ＋ compound fertilizer exhibited the plant height, stem diameter and yield increased by8.3%, 13.3% and 10.3%, respectively, and thus was the best; and the treatment showed a single curd weight of 478 g and a commodity rate of 89.4%, and had lower economic cost than treatment of 10‰ humic acid ＋ 6‰ PASP ＋ compound fertilizer. [Conclusion] In summary, 5‰ humic acid ＋ 3‰ PASP ＋ compound fertilizer is suitable for wide application in broccoli culture.

关键词： Humic acid PASP broccoli Growth Yield

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Plant Male Sterility Induced by Anti-Gene CYP86MF in Brassica oleracea var. italica

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Agricultural Sciences in China 2005年第11期4卷 806-810页

作者： HUANG Ke CAO Jia-shu YU Xiao-lin YE Wan-zhi LU Gang XIANG Xun Institute of Vegetable Science Zhejiang University Hangzhou 310029 P.R.China Research Center of Vegetable Science Fujian Academy of Agricultural Sciences Fuzhou 350013 P.R.China

An anti-gene CYP86MF was introduced into hypocotyls of broccoli （Brassica oleracea ***. italica Plenck） with Agrobacterium tumefaciens, and the transgenic plants were obtained by kanamycin selection. The results of PCR, Southern blot and Northern blot indicated that the anti-CYP86MF has been integrated into chromosome of the transgenic plant. And also, plants with hypogenetic stamina or ungerminated pollen were observed. The transgenic male sterility plant could fructify via artificial pollination with normal pollen. Thus it was proved that the pistil of male sterility plant was normally developed, and the sterility originated from anti-CYP86MF.

关键词： Brassica oleracea broccoli Anti-gene Male sterility

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Discovery and development of sulforaphane as a cancer chemopreventive phytochemical

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Acta Pharmacologica Sinica 2007年第9期28卷 1343-1354页

作者： Yuesheng ZHANG~2,Li TANGDepartment of Cancer Prevention and Control,Roswell Park Cancer Institute,Buffalo,New York 14263,USA Department of Cancer Prevention and Control Roswell Park CancerInstituteBuffaloNew York 14263USA

Sulforaphane (SF) is a phytochemical that displays both anticarcinogenic andanticancer *** modulates many cancer-related events,including suscep-tibility to carcinogens,cell death,cell cycle,angiogenesis,invasion and *** review its discovery and development as a cancer chemopreventive agentwith the intention of encouraging further research on this important compoundand facilitating the identification and development of new phytochemicals forcancer prevention.

关键词： chemoprevention phytochemical cyanate sulforaphane broccoli

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