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Pijx confers broad-spectrum seedling and panicle blast resistance by promoting the degradation of ATP β subunit and OsRbohC-mediated ROS burst in rice

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Molecular Plant 2023年第11期16卷 1832-1846页

作者： Ning Xiao Yunyu Wu Xiaoxiang Zhang Zeyun Hao Zichun Chen Zefeng Yang Yue Cai Ruyi Wang Ling Yu Zhiping Wang Yue Lu Wei Shi Cunhong Pan Yuhong Li Changhai Zhou Jianju Liu Niansheng Huang Guangqing Liu Hongjuan Ji Shuhao Zhu Shuai Fang Yuese Ning Aihong Li Institute of Agricultural Sciences for Lixiahe Region in Jiangsu Yangzhou Rice Experiment Station of the China Agricultural Research SystemJiangsu Key Laboratory of Crop Genomics and Molecular BreedingYangzhou 225009China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Key Laboratory of Plant Functional Genomics Ministry of EducationJiangsu Key Laboratory of Crop Genomics and Molecular BreedingAgricultural College of Yangzhou UniversityYangzhou 225009China

Rice blast,caused by the fungal pathogen Magnaporthe oryzae,is one of the most important diseases of rice.Utilization of blast-resistance genes is the most economical,effective,and environmentally friendly way to control the disease.However,genetic resources with broad-spectrum resistance(BSR)that is effective throughout the rice growth period are rare.In this work,using a genome-wide association study,we identify a new blast-resistance gene,Pijx,which encodes a typical CC-NBS-LRR protein.Pijx is derived from a wild rice species and confers BSR to M.oryzae at both the seedling and panicle stages.The functions of the resistant haplotypes of Pijx are confirmed by gene knockout and overexpression experiments.Mechanistically,the LRR domain in Pijx interacts with and promotes the degradation of the ATP synthase β subunit(ATPb)via the 26S proteasome pathway.ATPb acts as a negative regulator of Pijx-mediated panicle blast resistance,and interacts with OsRbohC to promote its degradation.Consistently,loss of ATPb function causes an increase in NAPDH content and ROS burst.Remarkably,when Pijx is introgressed into two japonica rice varieties,the introgression lines show BSR and increased yields that are approximately 51.59%and 79.31%higher compared with those of their parents in a natural blast disease nursery.In addition,we generate PPL^(Pijx Pigm) and PPL^(Pijx Piz-t) pyramided lines and these lines also have higher BSR to panicle blast compared with Pigm-or Piz-t-containing rice plants.Collectively,this study demonstrates that Pijx not only confers BSR to M.oryzae but also maintains high and stable rice yield,providing new genetic resources and molecular targets for breeding rice varieties with broad-spectrum blast resistance.

关键词： panicle blast broad-spectrum resistance Pijx ATPb ROS burst

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Achieving broad-spectrum resistance against rice bacterial blight through targeted promoter editing and pathogen population monitoring

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aBIOTECH 2020年第2期1卷 119-122页

作者： Jiyang Wang Yuese Ning Irene N.Gentzel Guo-Liang Wang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Department of Plant Pathology The Ohio State UniversityColumbusOH 43210USA

Plant diseases severely reduce crop yields and threaten global food security.broad-spectrum resistance(BSR)is a desirable trait because it confers resistance against more than one pathogen species or the majority of races/strains of the same pathogen.To control plant diseases,breeders have selected BSR to reduce disease occurrence and prolong the life-span of newly released cultivars in the last several decades(Mundt,Phytopathology 108(7):792–802,2018).Although effective,breeding of BSR cultivars in crop plants is still time-consuming and technically challenging.Recently,new gene-editing technologies such as CRISPR/Cas9 have dramatically accelerated the process of plant breeding and provided an approach for rapidly creating new varieties with BSR and other beneficial traits(Borrelli et al.,Front Plant Sci 9:1245,2018).In addition,close surveillance of pathogen populations in the field can provide useful information for the deployment of appropriate resistance genes in the target regions.In this mini-review,we focus on the significance and application of the exciting results from two recent companion papers published in Nature Biotechnology that provide new strategies to develop crop plants with BSR against pathogens through targeted promoter editing of susceptibility genes in plants as well as pathogen population monitoring.

关键词： CRISPR/Cas9 broad-spectrum resistance Rice bacterial blight Stacking editing Pathogen population monitoring

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Research Progress in Molecular Mechanism of broad-spectrum Disease resistance in Rice

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Agricultural Biotechnology 2021年第4期10卷 4-10,15页

作者： Jing SU Jinqi FENG Kailing CHEN Shen CHEN Xiaoyuan ZHU Plant Protection Research Institute Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of High Technology for Plant ProtectionGuangzhou 510640China

Yield loss caused by crop diseases seriously restricts global food security.Breeding disease-resistant varieties with resistance(R)genes is acknowledged as the most economical and effective way to control diseases.Therefore,exploring broad-spectrum resistance(BSR)genes to pathogens and clarifying the underlying mechanism are the basis for effective utilization of BSR resources and finding new approaches for disease control.In the past 20 years,significant progress has also been made in plant immune mechanism and remarkable achievements have been made in researches on broad-spectrum disease resistance of major grain crops,and the potential molecular mechanisms of some BSR have been revealed.Here,the advances of broad-spectrum disease resistance in rice(including the representative cloned BSR genes and their molecular mechanisms)were reviewed.In addition,the problems,opportunities and challenges encountered by BSR were analyzed,and the development of BSR research and its application in rice production were prospected.

关键词： Rice Disease broad-spectrum resistance Molecular mechanism

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Screening of broad-spectrum resistance Resources against Rice Bacterial Leaf Streak

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Plant Diseases and Pests 2017年第3期8卷 16-20页

作者： Hong Dengwei Zhao Yan Luo Dengjie Shi Lijun Li Rongbai Liu Fang College of Agriculture Guangxi University State Key Laboratory for Protection and Utilization of Subtropical Agro-bioresources

[ Objectives] Rice materials with broad-spectrum resistance against several pathogenic strains of Xanthomans oryzae pv. Oryzicola （Xooc） at multiple growth stages were screened, in order to provide reliable resistance sources for variety breeding against rice bacterial leaf streak. [ Methods] A total of 1 100 rice lines with rich genetic background were offered as selective objects, and highly susceptible cultivar Jingang 30 was used as the control. Inoculation was carried out with five strong pathogenic strains of Xooc through acupuncture method at various growth stages for resistance identification. [ Results] Fourteen disease-resistant materials were obtained through preliminary screening, accounting for 1.27% of the total materials. Nine materials moderately resistant to bacterial leaf streak were obtained via secondary screening, accounting for 0.82% of the total materials. Besides, three of them （ RL6, RL9 and RLL4） were resistant to a number of pathogenic strains of Xooc at three growth stages. In particular, RL16 presented broad-spectrum resistance to multi-strains with high resistance levels. Six of them （ RI2, RIA, RIS, RL8, RL11 and RL12） were resistant to single strain at single growth stage. [ Conclusions] The obtained three materials have broad-spectrum resistance to bacterial leaf streak at multiple growth stages, which can be served as an important source to cultivate disease-resistant rice varieties. RL6 maintains high broad-spectrum resistance at all growth stages, which can be used as a prior resistance source for rice variety breeding. Six materials obtained with resistance against bacterial leaf streak at particular growth stages can be used as candidate resistance sources.

关键词： Rice Bacterial leaf streak broad-spectrum resistance resistance resources

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Ortholog Alleles at Xa3/Xa26 Locus Confer Conserved Race-Specific resistance against Xanthomonas oryzae in Rice

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Molecular Plant 2012年第1期5卷 281-290页

作者： Hong-Jing Li Xiang-Hua Li Jing-Hua Xiao Rod A.Wing Shi-Ping Wang National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research(Wuhan)Huazhong Agricultural UniversityWuhan 430070China Arizona Genomics Institute School of Plant SciencesBIO5 InstituteUniversity of ArizonaTucsonAZ 85721USA

The rice disease resistance （R） gene Xa3/Xa26 （having also been named Xa3 and Xa26） against Xanthomonas oryzae pv. oryzae （Xoo）, which causes bacterial blight disease, belongs to a multiple gene family clustered in chromosome 11 and is from an AA genome rice cultivar （Oryza sativa L.）. This family encodes leucine-rich repeat （LRR） receptor kinase- type proteins, Here, we show that the orthologs （alleles） of Xa3/Xa26, Xa3/Xa26-2, and Xa3/Xa26-3, from wild Oryza spe- cies O. officinalis （CC genome） and O. minuta （BBCC genome）, respectively, were also R genes against Xoo. Xa3/Xa26-2 and Xa3/Xa26-3 conferred resistance to 16 of the 18 Xoo strains examined. Comparative sequence analysis of the Xa3/Xa26 families in the two wild Oryza species showed that Xa3/Xa26-3 appeared to have originated from the CC genome of O. minuta. The predicted proteins encoded by Xa3/Xa26, Xa3/Xa26-2, and Xa3/Xa26-3 share 91-99% sequence identity and 94-99% sequence similarity. Transgenic plants carrying a single copy of Xa3/Xa26, Xa3/Xa26-2, or Xa3PXa26-3, in the same genetic background, showed a similar resistance spectrum to a set of Xoo strains, although plants carrying Xa3/Xa26-2 or Xa3/Xa26-3 showed lower resistance levels than the plants carrying Xa3/Xa26. These results suggest that the Xa3/Xa26 locus predates the speciation of A and C genome, which is approximately 7.5 million years ago. Thus, the resistance spec- ificity of this locus has been conserved for a long time.

关键词： Bacterial blight broad-spectrum resistance durable resistance Oryza officinalis Oryza minuta Oryza sativa.

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Engineering broad-spectrum disease-resistant rice by editing multiple susceptibility genes

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Journal of Integrative Plant Biology 2021年第9期63卷 1639-1648页

作者： Hui Tao Xuetao Shi Feng He Dan Wang Ning Xiao Hong Fang Ruyi Wang Fan Zhang Min Wang Aihong Li Xionglun Liu Guo-Liang Wang Yuese Ning College of Agronomy Hunan Agricultural UniversityChangsha 410128China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Department of Plant Pathology The Ohio State UniversityColumbusOH43210USA Institute of Agricultural Sciences for Lixiahe Region in Jiangsu Yangzhou 225009China

Rice blast and bacterial blight are important diseases of rice(Oryza sativa)caused by the fungus Magnaporthe oryzae and the bacterium Xanthomonas oryzae pv.oryzae(Xoo),respectively.Breeding rice varieties for broadspectrum resistance is considered the most effective and sustainable approach to controlling both diseases.Although dominant resistance genes have been extensively used in rice breeding and production,generating diseaseresistant varieties by altering susceptibility(S)genes that facilitate pathogen compatibility remains unexplored.Here,using CRISPR/Cas9 technology,we generated loss-of-function mutants of the S genes Pi21 and Bsr-d1 and showed that they had increased resistance to M.oryzae.We also generated a knockout mutant of the S gene Xa5 that showed increased resistance to Xoo.Remarkably,a triple mutant of all three S genes had significantly enhanced resistance to both M.oryzae and Xoo.Moreover,the triple mutant was comparable to the wild type in regard to key agronomic traits,including plant height,effective panicle number per plant,grain number per panicle,seed setting rate,and thousand-grain weight.These results demonstrate that the simultaneous editing of multiple S genes is a powerful strategy for generating new rice varieties with broadspectrum resistance.

关键词： broad-spectrum resistance CRISPR/Cas9 gene editing Magnaporthe oryzae susceptibility gene Xanthomonas oryzae pv.oryzae

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Transcriptome analysis of Dlm mutants reveals the potential formation mechanism of lesion mimic in wheat

Transcriptome analysis of Dlm mutants reveals the potential ...

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中国植物病理学会第十二届青年学术研讨会

作者： Lei Li Xuan Shi Fei Zheng Di Wu Ai'ai Li Fayu Sun Changcheng Li Jincai Wu Tao Li Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops College of Plant Protection Yangzhou University

Previous investigations demonstrated that Chinese wheat cv.Ning7840 confers a high level of broad-spectrum resistance to rusts and powdery mildew diseases at the adult plant stage mediated by a spontaneously occurring hypersensitive reactionlike phenotype（also called lesion mimic,LM）.In this study,two EMS-induced mutants of cv.Ning7840,Dlml and Dlml,showed the LM deficiency phenotype.These two mutants were susceptible to powdery mildew.Transcription de novo sequencing of wheat flag leaves of Dlml,Dlml and wild type at heading stage when the LM trait could be observed on the leaves of wild-type were performed to decipher the molecular mechanisms underlying the phenotypes contrasting in the LM.The results suggest that the diterpenoid-related stress response acts as an indirect regulator of the LM phenotype and broad-spectrum resistance in Ning7840.Oxidative phosphorylation,proteasome,and chlorophyll were involved in the suppression of the LM expression in Dlm mutants.This provides new insight into understanding the molecular mechanism of LM and broad-spectrum resistance in wheat.

关键词： wheat Lesion mimic deficiency mutant broad-spectrum resistance diterpenoid biosynthesis oxidative phosphorylation

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