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Influence of Glyphaea brevis twig extract on nucleus, tight junctions and expression of inhibin-β, stem cell factor, and androgen binding protein in TM4 Sertoli cells

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Asian pacific Journal of Reproduction 2019年第4期8卷 157-166页

作者： Janet Olayemi Olugbodi Oladipupo David Oluwafemi Adeleke Ojo Afolabi Clement Akinmoladun Department of Biochemistry Bingham UniversityAbuja-Keffi RoadKaruNigeria Department of Medical Bioscience University of the Western CapeBellvilleCape TownSouth Africa Phytomedicine and Biochemical Toxicology Unit Department of BiochemistryAfe Babalola UniversityPMB 5454Ado-EkitiNigeria Phytomedicine Biochemical Pharmacology and Toxicology LaboratoriesDepartment of BiochemistrySchool of SciencesPMB 704The Federal University of TechnologyAkureNigeria

Objective: To examine the influence of Glyphaea (G.) brevis twig extract on the mitochondrial dehydrogenase activity, integrity of the tight junctions between adjacent cells, mitochondria, apoptosis, nucleus and expression of inhibin-β, stem cell factor, and androgen binding protein in TM4 Sertoli cells. Methods: TM4 cell line was used in this study as it exhibited properties similar to the Sertoli cells. TM4 Sertoli cells were exposed to G. brevis twig extract (0.1, 1.0, 10.0, 100.0, or 1000.0μg/mL) for 24, 48 and 72 h. Parameters studied included cell viability [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay], mitochondrial membrane potential (tetra methyl rhodamine ethyl ester dye), transepithelial electrical resistance, apoptosis (Annexin V Alexa Fluor?488/propidium iodide assay) and mRNA expression (quantitative reverse transcription polymerase chain reaction). Results: G. brevis twig extract had no cytotoxic impact on cell viability, thus, considerably increasing the activity of mitochondrial dehydrogenase enzyme after 24 and 72 h exposure. Transepithelial electrical resistance values revealed substantial (Pbrevis twig extract when compared to controls. In addition, G. brevis twig extract significantly reduced necrosis and apoptosis of TM4 Sertoli cells when compared to control. Nevertheless, fluorescence microscopy revealed that the nuclei were egg-shaped and marked uniformly with consistent cell shape at the middle of the TM4 Sertoli cells. Significant stimulatory effects were observed on mRNA levels of inhibin-β, androgen binding protein and stem cell factor. Conclusions: G. brevis twig extract may increase the secretory roles of TM4 Sertoli cells, cells proliferation, as well as cell-cell tight junction integrity. Thus, G. brevis twig may enhance spermatogenesis

关键词： Glyphaea brevis twig TM4 Sertoli cell line Inhibin-β Androgen binding protein

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TRANSPOSITION OF FLEXOR POLLICIS brevis MUSCLE FOR RECONSTRUCTION OF T

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Chinese Medical Journal 1996年第6期 21-24页

作者：朱伟王澍寰张友乐扬克非胡琪王海华

ＴＲＡＮＳＰＯＳＩＴＩＯＮＯＦＦＬＥＸＯＲＰＯＬＬＩＣＩＳＢＲＥＶＩＳＭＵＳＣＬＥＦＯＲＲＥＣＯＮＳＴＲＵＣＴＩＯＮＯＦＴＨＵＭＢＯＰＰＯＳＩＴＩＯＮＡＮＡＴＯＭＩＣＡＬＳＴＵＤＹＡＮＤＣＬＩＮＩＣＡＬＡＰＰＬＩＣＡ... 详细信息

ＴＲＡＮＳＰＯＳＩＴＩＯＮＯＦＦＬＥＸＯＲＰＯＬＬＩＣＩＳＢＲＥＶＩＳＭＵＳＣＬＥＦＯＲＲＥＣＯＮＳＴＲＵＣＴＩＯＮＯＦＴＨＵＭＢＯＰＰＯＳＩＴＩＯＮＡＮＡＴＯＭＩＣＡＬＳＴＵＤＹＡＮＤＣＬＩＮＩＣＡＬＡＰＰＬＩＣＡＴＩＯＮＺｈｕＷｅｉ朱伟，Ｗａｎｇ...

关键词：朱伟 MUSCLE POLLICIS OF RECONSTRUCTION FLEXOR brevis

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When free flaps are not the first choice: is the distally based peroneus brevis still an option for foot and ankle reconstruction in the era of microsurgery?

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Plastic and Aesthetic Research 2018年第7期5卷 26-34页

作者： Raymund E.Horch Ingo LudoIph Marweh Schmitz Anja M.Boos UIrich Kneser Justus P.Beier Andreas Arkudas1 Department of Plastic and Hand Surgery University Hospital ErlangenFriedrich-Alexander University Erlangen-Nuernberg FAUErlangen D-91054Germany Department of Hand Plastic and Reconstructive Surgery-Burn Center-BG Trauma Center LudwigshafenDepartment of Plastic SurgeryUniversity of HeidelbergLudwigshafen D-67071Germany Department of Plastic Hand and Burn Surgery University Hospital RWTHAachen D-52074Germany

Aim: Soft tissue defects with or without exposed bones in the lower extremity, ankle and the foot-with or without bone defects or exposed hardware-often require coverage with vascularized flaps. Free flaps, which add healthy tissue especially to the lower extremity instead of further injuring a limb, are the first choice in high volume microsurgical centres. Nevertheless, in some instances pedicled flaps may have indications when free flaps are not suitable. Methods: The distally based peroneus brevis flap is harvested from the lateral compartment of the leg based on the distal perforating arterial supply and covered with split skin. Results: We performed a total of 69 peroneus flaps between 2003 and 2017. Minor flap necroses at the distal tip were noted in 8% of the peroneus brevis reconstructions. Total flap loss occurred in 1 peroneus flap. Defect etiology and patient age were not associated with surgical outcome. Conclusion: While nowadays the first choice of lower extremity reconstruction is an appropriate free flap solution,the peroneus brevis muscle flap can also be seen as a valuable tool to reconstruct small to medium sized defects at the ankle, distal tibia, and the heel with an acceptable donor site morbidity. Despite the easily available variety of free flaps to achieve this purpose, still proper indications remain where a local flap can be a viable option in the hand of experienced plastic surgeons. However, caution is advisable in patients with peripheral arterial occlusive disease or venous insufficiency.

关键词： Free flaps peroneus brevis muscle flap lower extremity reconstruction

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Distally based peroneus brevis muscle flap: A single centre experience

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Chinese Journal of Traumatology 2019年第2期22卷 108-112页

作者： Subhash Sahu Amish Jayantilal Gohil Shweta Patil Shashank Lamba Kingsly Paul Ashish Kumar Gupta Department of Plastic & Reconstructive Surgery Christian Medical College & Hospital Vellore Tamilnadu India

Purpose: Defects aro und the distal one third of the leg and ankle are difficult to man age by con servative measures or simple split thickness skin graft. Distally based peroneus brevis muscle flap is a well described flap for such defects. Methods: This is a retrospective analysis conducted on 25 patients with soft tissue and bony defects of distal third of lower leg and an kle, which were treated using distally based per on eus brevis muscle flap from January 2013 to January 2018. Information regarding patient demographics, etiology, size and location of defects and complications were collected. All patients were followed up for at least 3 months after surgery. Results: There were 21 males and 4 females with the mean age of 39 (5-76) years. The most comm on cause of injuries was road traffic accident, followed by complicated open injury. The average size of defects was 20 (4—50) cm^2. The mean operating time was 75 (60-90) min for flap harvest and inset. We had no patient with complete loss of the flap. Five patients (20%) had marginal necrosis of the flap and two patie nts have graft loss due to un derlyi ng hematoma and required sec on dary split thickness skin grafting. Conclusion: The distally based peroneus brevis muscle flap is a safe optio n w 让h reliable an atomy for small to moderate sized defects following low velocity injury around the ankle. The commonest complication encountered is skin graft loss which can be reduced by primary delayed grafting.

关键词： Peroneus brevis flap Reverse sural artery Tendoachilles Lower leg defects

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Effect of <i>Lactobacillus brevis</i>SBC8803 on Gamma-Glutamyl Transferase in Japanese Habitual Drinkers: A Double-Blind, Placebo-Controlled Study

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Food and Nutrition Sciences 2012年第5期3卷 678-684页

作者： Yoshihisa Wakita Hajime Kanda Chikako Shimizu Yasukazu Nakakita Hirotaka Kaneda Shuichi Segawa Moeko Ozaki Tatsuro Shigyo Takaaki Ohtake Mikihiro Fujiya Yutaka Kohgo Corporate Planning Department Sapporo Holdings Ltd. Tokyo Japan Division of Gastroenterology and Hematology/Oncology Department of Medicine Asahikawa Medi- cal University Hokkaido Japan Frontier Laboratories of Value Creation Sapporo Breweries Ltd. Shizuoka Japan

A randomized, double-blind, placebo-controlled clinical trial in Japanese habitual drinkers was conducted to evaluate the efficacy of Lactobacillus brevis SBC8803 to alleviate adverse effect of alcohol. Subjects who drank habitually and had moderately higher levels of gamma-glutamyl transferase (GGT) (50 - 100 IU/L) were enrolled. The levels of transaminases in these subjects were almost within normal levels (aspartate transaminase (AST) brevis SBC8803 (n = 22) per day were administered for the continuous eight weeks (56 days). During the period, the subjects both in test group and placebo groups have kept each drinking behavior as usual. Regarding lipid metabolism, triacylglycerol (TG) levels in the male test group significantly decreased at week 4 as compared with week 0. Biomarkers of hepatocytes-damage;AST and ALT levels showed no significant differences between the pla- cebo and test groups at both weeks 4 and 8. Oxidative stress marker;GGT at weeks 4 was significantly lower in the test group than that in the placebo group (p = 0.017), but not at weeks 8. However, taking a reduced rate of GGT at weeks 8 comparing with that at week 0, that in the test group showed larger value comparing with that in the placebo group. These data about TG and GGT suggest that, although efficacy of L. brevis SBC8803 is limited in this study, intake of the probiotic may alleviate alcoholic influence in lipid metabolism and oxidative stress.

关键词： Lactobacillus brevis Gamma-Glutamyl Transferase Alcoholic Related Disorder Double-Blind Placebo-Controlled Study

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Bacillus brevis DX01 Labeling by Green Fluorescent Protein Gene and Its Colonization in Rice Seedling Roots

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Journal of Shanghai Jiaotong university(Science) 2005年第S1期10卷 26-31页

作者：陈云鹏沈大棱 Dept. of Plant Science Shanghai Jiaotong Univ. Shanghai 201101 China Inst. of Genetics State Key Lab. of Genetic Eng. School of Life Sciences Fudan Univ. Shanghai 200433

A constitutive expression vector pHY300-F1gfp was constructed to test the function of a promoter F1 subcloned from a rice epiphyte Bacillus brevis strain DX01. The DX01 cells harboring plasmid pHY300-F1gfp were detected to produce bright green fluorescence. Subsequently, the gfp-tagged B. brevis strain was released into the soil and its survival was investigated by PCR and the detection of green fluorescence. The spatial location of in situ gfp-tagged bacterial cells on the root surface of rice seedlings was visualized. All these results indicated that green fluorescent protein is an ideal molecular marker for detection of the activities of promoter F1, and it is also a reliable probe to monitor specific B. brevis bacteria in the environment.

关键词： green fluorescent protein Bacillus brevis colonization

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Heat-Killed <i>Lactobacillus brevis</i>SBC8803 Induces Serotonin Release from Intestinal Cells

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Food and Nutrition Sciences 2013年第8期4卷 767-771页

作者： Yasukazu Nakaita Hirotaka Kaneda Tatsuro Shigyo Corporate Planning Department Sapporo Holdings Ltd. Tokyo Japan. Frontier Laboratories of Value Creation Sapporo Breweries Ltd. Shizuoka Japan

Previously, we reported that changes induced in autonomic neurotransmission in rats by Lactobacillus brevis SBC8803 may be mediated by serotonin 3 (5-HT3) receptors. In this study, we evaluated the effects of heat-killed L. brevis SBC8803 on serotonin (5-HT) releasing from intestinal cells. In the in vitro study, L. brevis SBC8803 stimulated 5-HT release from cultured rat endocrine RIN-14B cells (SBC8803 vs. sterile water;P in vivo study, 2 mg of heat-killed L. brevis SBC8803 was administered using a stomach sonde (feeding needle) to C57BL/6J mice. Analysis of plasma by ELISA showed gradually increase in 5-HT concentrations (0 min vs. 60 min;P ex vivo cultured intestinal loops composed of duodenum and part of the jejunum, from C3H/HeN and C57BL/6J male mice indicated that L. brevis SBC8803 effectively induced 5-HT release (SBC8803 vs. sterile water;P L. brevis SBC8803 may stimulate 5-HT release from mouse intestinal cells such as enterochromaffin cells.

关键词： Serotonin Lactobacillus brevis Mice Intestinal Cells 5-HT3 Receptors

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大孔树脂静态吸附番茄内生菌Brevibacillus brevis W4抑细菌成分

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农药 2011年第11期50卷 802-804页

作者：杨从军张新刚史冠莹杨凌云陶科侯太平四川大学生命科学学院成都610064

[目的]Brevibacillus brevis W4是从番茄茎中分离得到的1株对灰霉病菌有强烈抑制作用的内生菌。为明确其发酵液抑细菌活性,并探索抑细菌物质的大孔树脂吸附条件。[方法]采用管碟法测定了*** W4发酵液对6种病原细菌的抑制活性。并以金黄... 详细信息

[目的]Brevibacillus brevis W4是从番茄茎中分离得到的1株对灰霉病菌有强烈抑制作用的内生菌。为明确其发酵液抑细菌活性,并探索抑细菌物质的大孔树脂吸附条件。[方法]采用管碟法测定了*** W4发酵液对6种病原细菌的抑制活性。并以金黄色葡萄球菌为指示菌,选用大孔树脂对发酵液中的抑细菌活性物质进行静态吸附条件优化。[结果]*** W4发酵液对供试枯草芽孢杆菌、金黄色葡萄球菌、水稻白叶枯病菌、柑橘溃疡病菌、大白菜软腐病菌、姜青枯假单孢杆菌均有抑制活性,对金黄色葡萄球菌和枯草芽孢杆菌的抑菌圈突出,直径分别为21.7、20.1 mm。大孔树脂AB-8对活性物质的吸附效果最好,其较优的静态吸附条件为上样液pH值为8.0,上样量为树脂质量的15倍,最佳的洗脱液体积分数为40%甲醇。[结论]*** W4发酵液具有抑制细菌作用,对金黄色葡萄球菌和枯草芽孢杆菌的抑制作用最强。AB-8可以作为抑细菌活性成分的吸附树脂。

关键词： Brevibacillus brevis W4 抑细菌活性大孔树脂静态吸附

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菌株LPF-1(Brevibacillus brevis LPF-1)降解猪粪过程中降解物质的GS-MS分析

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福建农业学报 2011年第6期26卷 1056-1064页

作者：蓝江林刘波陈峥史怀栗丰福建省农业科学院农业生物资源研究所福建福州350003

利用气-质联用(GS-MS)分析猪粪污降解菌LPF-1(Brevibacillus brevis LPF-1)降解猪粪过程中挥发性物质的变化。以匹配度大于80(最大为100),含量大于5%为标准,在3个处理中共检测到各类物质23种,其中菌株LPF-1发酵液检测到的物质种类最多,... 详细信息

利用气-质联用(GS-MS)分析猪粪污降解菌LPF-1(Brevibacillus brevis LPF-1)降解猪粪过程中挥发性物质的变化。以匹配度大于80(最大为100),含量大于5%为标准,在3个处理中共检测到各类物质23种,其中菌株LPF-1发酵液检测到的物质种类最多,有16种;猪粪+水溶液处理检测到12种,其中苯类物质3种(1,2-二甲苯、对二甲苯、间二甲苯),酚类物质4种(2,4-二叔丁基苯酚、苯酚、2,5-二叔丁基酚、4-甲基苯酚);猪粪+LPF-1发酵液处理检测到物质10种,其中苯类物质1种(对二甲苯),酚类物质3种(2,4-二叔丁基苯酚、苯酚、4-甲基苯酚),菌株LPF-1能有效减少猪粪降解过程中苯类和酚类等有害物质种类的产生,具有进一步开发成粪污降解微生物菌剂潜力。

关键词： Brevibacillus brevis LPF-1 猪粪 GS-MS

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亚硝酸盐影响Lactobacillus brevis 4903发酵的研究

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微生物学杂志 2005年第3期25卷 25-27页

作者：张庆芳迟乃玉薛景珍魏毓棠大连大学生命科学工作室大连116622 大连大学生物工程学院大连116622 沈阳农业大学食品学院辽宁沈阳116622

通过研究可知,亚硝酸盐对Lactobacillusbrevis4903发酵有抑制作用,环境中亚硝酸盐一旦分解掉,这种抑制作用就会被解除。分析其原因:①亚硝酸盐抑制了乳酸菌生长,从而抑制了乳酸发酵;②在发酵初期可能因亚硝酸盐还原酶的作用,使亚硝酸盐... 详细信息

通过研究可知,亚硝酸盐对Lactobacillusbrevis4903发酵有抑制作用,环境中亚硝酸盐一旦分解掉,这种抑制作用就会被解除。分析其原因:①亚硝酸盐抑制了乳酸菌生长,从而抑制了乳酸发酵;②在发酵初期可能因亚硝酸盐还原酶的作用,使亚硝酸盐酶解生成NH3,NH3中和了乳酸菌生成的酸(H+),从而使环境pH值的下降和酸的积累变得缓慢。

关键词：亚硝酸盐 Lactobacillus brevis 4903 乳酸发酵

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