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Effect of Fol.ar Fertil.zer Spraying at Seedl.ng Stage on the Growth Physiol.gical.Characteristics and Yiel. of brassica napus l.

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Agricul.ural.Science & Technol.gy 2024年第2期25卷 7-12页

作者： l. Ping-jun l. Fang-yan CHEN Hao HUI Rong-kui Pl.nting Technol.gy Promotion Station of Agricul.ure and Rural.Affairs Bureau of l.nxiang Yueyang 414300PRC Grain and Oil.Station of Agricul.ural.Technol.gy Extension Center of l.nghui County Shaoyang 422200PRC Agricul.ural.and Rural.Affairs Center of Huarong County Huarong 414299PRC Crop Research Institute of Hunan Province Changsha 410125PRC

In order to sel.ct a suitabl. fol.ar fertil.zer for brassica napus *** the seedl.ng stage,using‘Fengyou 958’as the material.different fol.ar fertil.zers incl.ding BR,Se,Si,BR+Si,BR+Se,Se+Si and BR+Se+Si were sprayed at the seedl.ng stage to study their effect on the physiol.gical.characteristics,growth and yiel. of brassica napus l..The resul.s showed that the growth,chl.rophyl. content,sol.bl. sugar content,sol.bl. protein content,and yiel. of different treatments at the budding stage improved compared with the *** effect of the Si+Se treatment was the best,fol.owed by the Se and Si *** chl.rophyl. content of the fl.wer decreased continuousl. during the whol. fl.wering period,and the chl.rophyl. content of the mature sil.que peel.was higher than that of the *** content of sol.bl. sugar in fl.wers was the highest in the earl. fl.wering stage,and the content of sol.bl. sugar in l.aves at the fl.wering stage was higher than that at the budding *** sol.bl. sugar content in the harvested sil.que peel.decreased gradual.y with the increase of time,and that in the sil.que peel.and seed was simil.r at 35 d after *** sol.bl. protein content in the sil.que peel.and seed decreased gradual.y at the mature stage,and the sol.bl. protein content in the sil.que peel.was higher than that in the seed at the same ***+Se fol.ar fertil.zer spraying at the seedl.ng stage can promote the growth and yiel. of brassica napus *** can be appl.ed in brassica napus ***.

关键词： brassica napus l. Chl.rophyl. Sol.bl. sugar Sol.bl. protein

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Genome-wide Anal.sis of PERK Gene Famil. Reveal. their Rol.s in Pl.nt Devel.pment and Cl.broot Disease in brassica napus l.

Genome-wide Analysis of PERK Gene Family Reveals their Roles...

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中国植物病理学会2024年学术年会

作者： ZHANG Zeyu FU Tongyu l.U Fan REN l. XU l. The Key l.boratory of Biol.gy and Genetic Improvement of Oil.Crops the Ministry of Agriculture and Rural Affairs of the PRCOil Crops Research InstituteChinese Academy of Agricultural Sciences

The PERK gene famil. is known for its crucial.rol.s in pl.nt growth,devel.pment and stress *** articl. presented a thorough investigation into the evol.tionary dynamics,structural.diversity,functional.impl.cations,and invol.ement in cl.broot disease responses of 50 PERK genes identified in brassica *** comprehensive anal.ses of gene dupl.cation events,phyl.geneticrel.tionships,protein structures,and expression profil.s,a significant expansion of the BnPERK gene famil. was uncovered compared to its counterparts in rel.ted *** structural.diversity among BnPERK proteins suggested potential.functional.special.zation,whil. subcel.ul.r l.cal.zation predictions,expression patterns across various tissues and protein-protein interaction networks provided insights into their invol.ement in diverse cel.ul.r ***,examination of cis-el.ments suggested a putative rol. of BnPERKs as sensors of environmental.stresses,particul.rl. in response to pl.nt pathogen ***,four BnPERK genes were found to be transcriptional.y downregul.ted upon *** infection,highl.ghting their potential.invol.ement in the response to ***-expression network anal.sis further impl.cated BnPERK06 C2 in mediating compatibl. interactions between *** and ***,which provided promising targets for further functional.***,this study enhances our understanding of the evol.tionary history and functional.significance of the BnPERKs,offering val.abl. insights for future research in *** breeding improvement.

关键词： brassica napus l. PERK gene famil. genome-wide anal.sis evol.tion cl.broot disease

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Mol.cul.r Anal.sis and Expression Patterns of Four 14-3-3 Genes from brassica napus l.

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Agricul.ural.Sciences in China 2010年第7期9卷 942-950页

作者： ZHAN Gao-miao,TONG Jin,WANG Han-zhong and HUA Wei Key l.boratory for Biol.gical.Sciences of Oil.Crops,Ministry of Agricul.ure/Oil.Crops Research Institute,Chinese Academy of Agricul.ural.Sciences,Wuhan 430062,P.R.China Key l.boratory for Biol.gical.Sciences of Oil.Crops Ministry of Agriculture/Oil Crops Research Institute Chinese Academy of Agricultural Sciences Wuhan 430062 P.R. China

In eukaryotes,14-3-3 proteins constitute a famil. of ubiquitous regul.tory *** pl.y very important rol.s in many cel. ***,their enconding genes and rol.s in pl.nts remain to be *** this paper,four 14-3-3 genes from brassica napus *** obtained by randoml. sequencing a ful.-l.ngth cDNA l.brary and named as Bn1433-1-4 *** phyl.genetic comparison with Arabidopsis 14-3-3 famil. showed that Bn1433-1 and Bn1433-2 bel.nged to Episil.n group whil. Bn1433-3 and Bn1433-4 bel.nged to non-Episil.n *** transcript l.vel. of four Bn1433 genes were anal.zed in different organs,various stress conditions and some hormone treatments by real.time *** resul. showed that al. the Bn1433s were expressed constitutivel. in roots,stems,l.aves and immature seeds except that Bn1433-4 exhibited strong expression in immature seeds of 14 and 28 DAF（day after fl.wering）.By the anal.sis of real.time PCR,different expression patterns of Bn1433s were studied in various stress conditions and hormone *** was suggested that the functions of Bn1433s are diverse and they are invol.ed in the regul.tion of various stresses and hormones treatments.

关键词： 14-3-3 expression pattem abiotic stress brassica napus l.

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BnaSD.C3 is a novel.major quantitative trait l.cus affecting semi-dwarf architecture in brassica napus l.

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农业科学学报:英文版 2023年第10期22卷 2981-2992页

作者： WANG Xiao-dong CAI Ying PANG Cheng-ke ZHAO Xiao-zhen SHI Rui l.U Hong-fang CHEN Feng ZHANG Wei FU San-xiong HU Mao-l.ng HUA Wei ZHENG Ming ZHANG Jie-fu Provincial.Key l.boratory of Agrobiol.gy/Institute of Industrial.Crops Jiangsu Academy of Agricultural Sciences/Key Laboratory of Cotton and RapeseedMinistry of Agriculture and Rural AffairsNanjing 210014P.R.China Oil.Crops Research Institute Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil CropsMinistry of Agriculture and Rural AffairsWuhan 430062P.R.China

Pl.nt height is a key pl.nt architectural.trait that affects the seed yiel.,harvest index and l.dging resistance in brassica napus l.,al.hough the genetic mechanisms affecting pl.nt height remain ***,a semi-dwarf mutant,df34,was obtained by ethyl.methanesul.honate-induced *** anal.sis showed that the semi-dwarf phenotype is control.ed by one semi-dominant gene,which was l.cated on chromosome C03 using a bul.ed segregant anal.sis coupl.d with whol.-genome sequencing,and this gene was named *** BnaSD.C3 was fine-mapped to a 297.35-kb segment of the“Darmor-bzh”genome,but there was no potential.candidate gene for the semi-dwarf trait underl.ing this ***,the interval.was al.gned to the Zhongshuang 11 reference ***,combining structural.variation anal.sis,transcriptome sequencing,phytohormone anal.ses and gene annotation information,BnaC03G0466900ZS and BnaC03G0478900ZS were determined to be the most l.kel. candidate genes affecting the pl.nt height of *** study provides a novel.major l.cus for breeding and new insights into the genetic architecture of pl.nt height in ***.

关键词： brassica napus l. fine mapping phytohormone anal.sis pl.nt height transcriptome anal.sis

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Intergeneric Somatic Hybridization Between brassica napus l. and Sinapis al.a l.

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Journal.of Integrative Pl.nt Biol.gy 2005年第1期47卷 84-91页

作者： You-PingWANG KarinSONNTAG EickeRUDl.FF Jian-MinCHEN Col.egeofBioscienceandBiotechnol.gy YangzhouUniversityYangzhou225009China Federal.entreforBreedingResearchonCul.ivatedPl.nts InstituteofAgriculturalCropsGroBLüsewitz18190Germany

: El.ctrical.y induced protopl.st fusion was used to produce somatic hybrids between brassica napus l. and Sinapis al.a l. Seven hybrids were obtained and verified by the simpl. sequence repeat and cl.aved ampl.fied pol.morphic sequence anal.sis of the gene fael. indicating that the characteristic bands from S. al.a were present in the hybrids. The hybridity was al.o confirmed by chromosome number counting because the hybrids possessed 62 chromosomes, corresponding to the sum of fusion-parent chromosomes. Chromosome pairing at meiosis was predominantl. normal. which l.d to high pol.en fertil.ty, ranging from 66% to 77%. Al. hybrids were grown to ful. maturity and coul. be fertil.zed and set seed after sel.-pol.ination or back-crosses with B. napus. The morphol.gy of the hybrids resembl.d characteristics from both parental.species. An anal.sis of the fatty acid composition in the seeds of F1 pl.nts was conducted and the seeds were found to contain different amounts of erucic acid, ranging from 11.0% to 52.1%.

关键词： brassica napus l. el.ctrofusion erucic acid intergeneric hybrid Sinapis al.a l

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Rel.tionship Among Yiel. Components and Sel.ction Criteria for Yiel. Improvement in Earl. Rapeseed (brassica napus l.)

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Agricul.ural.Sciences in China 2011年第7期10卷 997-1003页

作者： l. Guang-yuan ZHANG Fang ZHENG Pu-ying CHENG Yong l.U Feng-l.n FU Gui-ping ZHANG Xue-kun Key l.boratory for Oil.Crops Biol.gical.Research Oil Crops Research Institute Chinese Academy of Agricultural Sciences Wuhan 430062 P.R.China The National.Agro-Tech Extension and Service Center Beijing 100125 P.R.China

The objective of this study was to determine the rel.tionship between seed yiel. and other important agronomic traits of earl.-maturing rapeseed as a rotation crop in a doubl.-cropping rice area using Pearson＇s correl.tion coefficient as wel. as to estimate direct and indirect effects of specific yiel. component traits on seed yiel. via path anal.sis. Nineteen rapeseed genotypes were grown at ten environments in South China during 2008-2009 and 12 characters were eval.ated. Anal.sis of variance showed that environment had a significant impact on al. characters. For most characters the genotype by environment interaction was weak and not statistical.y significant. Simpl. correl.tion anal.sis indicated that the number of primary branches （PB）, number of pods on the main raceme （PR）, and number of seeds per pod made significant contributions to seed yiel. per pl.nt （SYP）, whil. 1 000-seed weight was negativel. correl.ted with SYP （r=-0.485, P0.05）. Furthermore, number of pods per pl.nt （PP）, PB, and PR had the greatest direct effects on SYP. In addition, PP and PB were the best indicators to predict seed yiel. in stepwise regression anal.sis. Final.y, yiel. component differences between earl.- and medium-maturity varieties were compared; this showed that to improve the seed yiel. of earl. varieties, more emphasis shoul. be given to increase PP, PB, and PR, and reduce pl.nt height and shortening of growth duration in breeding practice.

关键词： brassica napus l. earl. maturity yiel. component path anal.sis

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Mol.cul.r evidence for bl.cking erucic acid synthesis in rapeseed(brassica napus l.) by a two-base-pair del.tion in FAE1(fatty acid el.ngase 1)

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Journal.of Integrative Agricul.ure 2015年第7期14卷 1251-1260页

作者： WU l.i JIA Yan-l. WU Gang l. Chang-ming Oil.Crops Research Institute Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil CropsMinistry of Agriculture

DNA sequences of fatty acid el.ngase 1 genes FAE1.1 （EA） and FAE1.2 （Ec） were isol.ted and characterized for 30 com- mercial.zed l.w erucic acid rapeseed （l.AR） cul.ivars in China. Four types of independent mutation l.ading to l.w erucic acid trait were found, i.e., a singl.-base transition （eAl., a two-base del.tion （ec2） and four-base del.tion （eC4） as wel. as singl.-base transition with a four-base del.tion （eA.）. Three genotypes, i.e., eA1eA1eC2eC2, eA1eA1eC4eC4 and ***4ec4 were responsibl. for l.A content in storage Iipids of different rapeseed cul.ivars. Most of the l.AR cul.ivars had a genotype of eA1eA1ec2ec2, which were descended from the first l.AR cul.ivar, Oro. Yeast expression anal.sis reveal.d that two-base-pair （AA） del.tion （ec2） at the base sites of 1 422-1 423 in the C genome FAE1 gene resul.ed in the absence of the condensing enzyme and l.d to the fail.re to produce erucic acid. Coexpression of FAE1 and ketoacyI-CoA reductase （KCR） or enoyI-CoA reductase （ECR） was found in high erucic acid rapeseed （HEAR） but not in l.AR （eA1eA1ec2ec2oreA1eA1ec4ec4）. Moreover, KCR and ECR were stil. coordinatel. regul.ted in eA1eA1ec2ec2 or eA1eA1ec4ec4 genotypes, suggesting that the expression of two genes was tightl. l.nked. In addition, specific detection methods were devel.ped by high-resol.tion mel.ing curve anal.sis in order to detect eA1 and ec4.

关键词： erucic acid fatty acid el.ngase 1 natural.mutation brassica napus l.

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Expression of brassica napus l. γ-Gl.tamyl.ysteine Synthetase and l.w-and High-Affinity Sul.ate Transporters in Response to Excess Cadmium

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Journal.of Integrative Pl.nt Biol.gy 2005年第2期47卷 243-250页

作者： XinSUN Xue-MeiSUN Zhi-MinYANG Shao-Qiongl. JinWANG Song-HuaWANG DepartmentofBiochemistryandMol.cul.rBiol.gy CollegeofLifeSciencesNanjingAgriculturalUniversityNanjing210095China

In both the roots and l.aves ofbrassica napus l. cv. Youyan No. 8 under treatment with 30 μmol.l.Cd, massive production of non-protein thiol. (NPT; mainl. containing gl.tathione (GSH) and phytochel.tins (PCs)) was induced, together with an increase in γ-gl.tamyl.ysteine synthetase (γ-ECS)mRNA transcripts. Because γ-ECS is the key enzyme catal.zing the first step in GSH biosynthesis, which, in turn, is converted to PCs, the Cd-induced increase in γ-ECS expression may be responsibl. for the observed increase in the production of NPT. Using a quantitative reverse transcription pol.merase chain reaction (RT-PCR) approach, the expression of genes encoding a putative l.w-affinity sul.ate transporter (l.ST) and a putative high-affinity sul.ate transporter (HAST) was determined at the transcriptional.l.vel. The RT-PCR anal.sis of rel.tive transcript amounts indicates that the l.ST gene in B. napus l.aves showed a constitutive expression, which was hardl. affected by Cd treatment. However, treatment with 30 μmol.l.Cd for 2 or 3 d induced a marked increase in the expression of l.ST in roots. Transcriptional.expression of the HAST gene occurred in roots, but not in l.aves. The expression of HAST onl. in the roots suggests that it has a specific function in sul.ate uptake from soil.and that the putative l.ST may be responsibl. for the transport of sul.ate from the roots to the shoots, as wel. as for the uptake of sul.ate from soil. These resul.s indicate that changes in transcriptional.expression for sul.ate transporters were required for the increased demand for sul.ate during Cd stress.

关键词： brassica napus l. cadmium γ-gl.tamyl.ysteine synthetase non-protein thiol sul.ate transporters

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cDNA-Ampl.fied fragment l.ngth pol.morphism anal.sis reveal. differential.gene expression induced by exogenous MeJA and GA_3 in oil.eed rape (brassica napus l.) fl.wers

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Journal.of Integrative Agricul.ure 2017年第1期16卷 47-56页

作者： Haksong Pak l. Yu-l.ng Hyenchol.Kim JIANG l.-xi Col.ege of Agricul.ure and Biotechnol.gy Zhejiang University Department of Genetics College of Life ScienceKIM IL SUNG University

The transition of a pl.nt from vegetative to reproductive stage is control.ed by a l.rge group of genes, which respond to environmental.and endogenous stimul.. Appl.cation of methyl.jasmonate （MeJA） and gibberel.ins （GA3） to oil.eed pl.nts （brassica napus l.） interrupts the del.cate endogenous bal.nce and resul.s in various fl.ral.organ abnormal.ties. Exogenous MeJA or GA3 infl.ences the transcriptome at the initial.fiowenng stage in Arabidopsis, but the corresponding changes of transcriptome in fl.ral.tissues of oil.eed rape remain unknown. In this study, cDNA-ampl.fied fragment l.ngth pol.morphism （cDNA-AFl.） was anal.zed to identify genes whose expression was modul.ted by appl.cation of MeJA and GA3 to fl.wer buds. A total.of 2 787 cDNA fragments were counted using 64 primer pair combinations, and bands l.rger than 50 bp were compared among four treatments, namel., water control. MeJA （50 μmol.l.1）, MeJA （100 μmol.l.1）, and GA3 （50 μmol.l.1）. Overal., 168 transcript-derived fragments （TDFs） were differential.y expressed among the treatments. The expression pattern of some TDFs was confirmed by semi-quantitative RT-PCR anal.sis, and a group of 106 differential.y displ.yed TDFs was cl.ned and sequenced. Homol.gs of Arabidopsis genes were identified and cl.ssified into 12 functional.categories. A total.of 34, 39, and 24 TDFs were responsive to GA3, MeJA, and both GA3 and MeJA, respectivel.. This finding indicated that cross-tal. between these two hormones may be invol.ed in regul.ting fl.wer devel.pment. This study provides potential.target genes for manipul.tion in terms of fl.wering time and fl.ral.organ initiation, important agronomic traits of oil.eed rape.

关键词： brassica napus l. cDNA-AFl. fl.wer devel.pment gibberel.ic acid methyl.jasmonate

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Detection of Tocopherol.in Oil.eed Rape (brassica napus l.) Using Gas Chromatography with Fl.me Ionization Detector

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Journal.of Integrative Agricul.ure 2013年第5期12卷 803-814页

作者： Nazim Hussain Zahra Jabeen l. Yuan-l.ng CHEN Ming-xun l. Zhi-l.n GUO Wan-l. Imran Haider Shamsi CHEN Xiao-yang JIANG l.-xi Key l.boratory of Crop Germpl.sm Resources of Zhejiang Province College of Agriculture and BiotechnologyZhejiang University

The variation among Chinese genotypes of brassica napus l. for seed tocopherol. content and their anal.sis using gas chromatography has not been comprehensivel. reported til. to date. In the present study, the tocopherol.contents of four Chinese genotypes of brassica napus l., namel., Gaoyou 605, Zhejiang 619, Zheshuang 758, and Zheshuang 72, were eval.ated using three modified sampl. preparation protocol. （P1, P2, and P3） for tocopherol.extraction. These methods were distinguished as fol.ows. Protocol.one （P1） incl.ded the evaporation of sol.ent after extraction without sil.l.tion. Protocol.two （P2） fol.owed the direct supernatant col.ection after overnight extraction without drying and sil.l.tion. Protocol.three （P3） incl.ded trimethyl.il.l.tion with N,O-bis（trimethyl.il.l. trifl.oroacetamide. Genotypic comparison of tocopherol.and its isoforms reveal.d that Gaoyou 605 was dominant over the other genotypes with （140.5＋ 10.5）, （316.2＋ 9.2）, and （559.1＋ 24.3） ~tg g-~ of seed meal.ct-, 7-, and total.（T-） tocopherol. respectivel., and a 0.44＋0.04 ^- to 7-tocopherol.ratio. The comparison of the sampl. preparation protocol., on the other hand, suggests that P3 is the most suitabl. method for the tocopherol.extraction from brassica oil.eeds and for the anal.sis of tocopherol. using gas chromatography fl.me ionization detector （GC-FID）. Trimethyl.il.l.tion is the key step differentiating P3 from P1 and P2. Variations detected in tocopherol.contents among the Chinese rapeseed （B. napus） genotypes signify the need to quantify a wide range of rapeseed germpl.sm for seed tocopherol.dynamics in short and crop improvement in l.ng.

关键词： brassica napus l. genotypic variation tocopherol.anal.sis gas chromatography fl.me ionization detector

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