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Development and evaluation of immunoassay for zeranol in bovine urine

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第12期8卷 900-905页

作者： LIU Yuan ZHANG Cun-zhen YU Xiang-yang ZHANG Zhi-yong ZHANG Xiao LIU Rong-rong LIU Xian-jin GONG Zhen-ming Institute of Food Safety Jiangsu Academy of Agricultural Sciences Nanjing 210014 China School of Agriculture and Biology Shanghai Jiao Tong University Shanghai 200030 China

A high affinity polyclonal antibody-based enzyme linked immunosorbent assay （ELISA） was developed for the quantification of zeranol in bovine urine. On the basis of urine matrix studies, the optimized dilution factors producing insignificant matrix interference were selected as 1：5 in pretreatment. In the improved ELISA, the linear response range was between 0.02 and 1 μg/ml, and the detection limit was 0.02 μg/ml for the assay. The overall recoveries and the coefficients of variation （CVs） were in the range of 82%-127% and 3.5%-8.8%, respectively. Thirty-six bovine urine samples spiked with zeranol （ranging from 0.2 to 10 μg/ml） were detected by the ELISA and liquid chromatography （LC） method, and good correlations were obtained between the two methods （R^2=0.9643）. We conclude that this improved ELISA is suitable tool for a mass zeranol screening and can be an altemative for the conventional LC method for zeranol in bovine urine.

关键词： Zeranol Enzyme linked immunosorbent assay (ELISA) bovine urine

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液相色谱-串联质谱法测定牛尿中多种非类固醇同化激素残留量

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分析测试学报 2007年第z1期26卷 93-95页

作者：许泓庞国芳林安清古珑何佳张曼张骏林娜天津出入境检验检疫局天津300201 秦皇岛出入境检验检疫局河北秦皇岛066002

bovine urine was *** the internal standard(ZER-d4 and DES-d8) into the *** extract was cleaned up by two immunoaffinity columns respectively.A C8 analytical column was used and the analytes were chromatographed by acetonitrile-water(70:30,v/v).With HPLC-MS/MS,data acquisition was achieved by using atmospheric pressure chemical ionization (APCI) in negative scan mode and multiple reaction monitoring (MRM) determination *** was made by internal standarad *** limits of determination of DES and DEN are 0.05 ng·g-1,the recovery level was 80 %-106% between 0.5 and 10 ng·*** limits of determination of HEX and ZER are 0.025 ng·g-1,the recovery level was 83%-104% between 0.25 and 5 ng·g-1.

关键词： HPLC-MS/MS bovine urine Nonsteroid anabolic hormones Residue

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