检索结果-南通市图书馆

Chinese Medical Sciences Journal 1995年第2期10卷 93-95页

作者：连伟罗慰慈 Peking Union Medical College Beijing.

Polymerase chain reaction (PCR) was used to detect the presence of Barrelia burgdoferi DNA in biological samples from patients with sarcoidosis. The target DNA sequence was of chromosomal origin. The amplified DNA sequence was analyzed by agarose gel electrophoresis, PAGE with silver staining, and the identity of amplified DNA was confirmed by restriction enzyme cleavage and DNA-DNA hybridization with a 32P-labelled probe. The assay was sensitive to fewer than two copies of B. burgdorferi genome, even in the presence of a 104-fold excess of human eukaryotic DNA , and was also specific to different B. burgdorferi strains tested. Sera serologically positive to B. burgdcrferi (n=26), bronchoalveolar lavage fluid and supernatant of BALF (n=26) and peripheral blood (n=9) from sarcoidosis patients were tested. The positive rate was low (4/26, 2/26. and 0/9, respectively). It was considered that DNA from B. burgdor ferimay be identified in a minority of patients with sarcoidosis, and it may play a pathogenetic role in such cases. More studies need to be done before advancing the hypothesis of an etiologic role of B. burgdorferi in sarcoidosis.

关键词： sarcoidosis borrelia burgdorferi polymerase chain reaction (PCR)

维普期刊数据库

Nitroxoline Drug Combinations Are More Active Than Lyme Antibiotic Combination and Can Eradicate Stationary-Phase borrelia burgdorferi

在线全文

学校读者我要写书评

暂无评论

Infectious Microbes & Diseases 2022年第3期4卷 124-130页

作者： Hector S.Alvarez-Manzo Yumin Zhang Ying Zhang Department of Molecular Microbiology and Immunology Bloomberg School of Public HealthJohns Hopkins UniversityBaltimoreMarylandUSA State Key Laboratory for the Diagnosis and Treatment of Infectious Diseases The First Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiangChina

Lyme disease(LD),caused by borrelia burgdorferi,is themost common vector-borne disease in the United States and *** the standard 2–4 weeks'antibiotic treatment,approximately 10%–20%of patients will develop posttreatment LD syndrome,a condition that is poorly *** of the probable causes is thought to be the presence of *** persister forms that are not effectively killed by the current LD *** this study,we evaluated nitroxoline,an antibiotic used to treat urinary tract infections,for its activity against a stationary-phase culture enriched with persister forms of *** was found to be more active than doxycycline and equally active as cefuroxime(standard LD antibiotics)against ***,the nitroxoline two-drug combinations nitroxoline+cefuroxime and nitroxoline+clarithromycin,as well as the nitroxoline three-drug combination nitroxoline+cefuroxime+clarithromycin,were as effective as the persister drug daptomycin-based positive control three-drug combination cefuroxime+doxycycline+daptomycin,completely eradicating stationary-phase *** in the drug-exposure experiments and preventing regrowth in the subculture *** studies should evaluate these promising drug combinations in a persistent LD mouse model.

关键词： Lyme disease borrelia burgdorferi treatment nitroxoline drug repurposing

Introduction, Sign, Symptoms, Prevention and Management of Lyme Disease Caused by borrelia burgdorferi Channeled through Ixodes Ticks as Vector. A Review

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Entomology 2016年第5期4卷 249-259页

作者： Fakhra Tabbasam Muhammad Faheem Malik Usman Asghar Kamaran Saeed Paracha Takhmina Nazir University of Gujrat Ghakkhar Pakistan

God has created many miraculous creature;ticks are one of them. borrelia burgdorferi is a bacterium that lives into the gut of ticks. When such ticks feed upon the blood of Ticks and other livestock, bacteria are transferred into their blood stream of these hosts. A disease is caused into human beings if such infected tick bites humans that are called as Lyme disease. This review will help to have a keen study on the cause, sign, symptoms, prevention and management of this disease.

关键词： borrelia burgdorferi Livestocks

Genetic Characterization of Four Strains borrelia burgdorferi Isolated in China

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Microbiology and Immunology 2004年第1期2卷 6-9页

作者：曾霞王树声张涛毕胜利周永东 Guangxi Center for Disease Control and Prevention Institute of viral Disease Control Chinese Center for Disease Control and Prevention

To study the genetic characterization of four strains of borrelia burgdorferi isolated in China. PCR technique was used to amplify the 5S-23S rRNA intergenic spacer DNA from the whole cellular DNA of isolated GXLD-4, 9, 18 and Chang 14, and then the amplified products were cloned into plasmid pGEM-T Easy and sequenced. It was found that the 5S-23S rRNA intergenic spacer DNA of the four isolates was 242?bp, revealing the nucleotide sequence identity of more than 99%. The four isolates had higher sequence identify with borrelia valaisiana than with other genetic groups. These four isolates most likely belong to borrelia valaisiana genomic group.

关键词： borrelia burgdorferi The 5S-23S rRNA intergenic spacer DNA Cloning Sequence analysis

维普期刊数据库

Lyme Disease in Iraq： First Detection of IgM Antibodies to borrelia burgdorferi in Human Sera

在线全文

学校读者我要写书评

暂无评论

Journal of Life Sciences 2013年第11期7卷 1143-1146页

作者： Khalis A. Hamad Ameen Basima A. Abdullah Riyad A. Abdul-Razaq Department of Plant Protection College of Agriculture University of Salahaddin-Erbil Erbil Iraq Department of Biology (Microbiology) College of Science University of Mosul Mosul Iraq Department of Biology College of Science University of Mosul Mosul Iraq

The sera of 180 human samples were tested for the presence of antibodies against borrelia burgdorferi using the ELISA （enzyme-linked immunosorbent assay） technique. Out of 180 sero-samples, 46 （25.55%） were positive. Females of the age range 18-35 years had the highest rate of sero-positive samples 14 （38.88%）, while the highest percentage of sero-negative samples was found in males of the age range 50-80 years. The other sero-positive samples were： 6 （26.08%）, 6 （25%） and 3 （11.53%） in males of ages between 18-35, 35-50 and 50-80 years, respectively, and 11 （29.72%） and 6 （17.64%） in females in the age ranges 35-50 and 50-80 years, respectively. The mean concentration of Anti- B. burgdorferi antibody was higher （16.7 U/mL） when compared with mean concentration of normal value （5.5 U/mL）, P 〈 0.001.

关键词： borrelia burgdorferi IgM antibodies Erbil Iraq ELISA

Identification and molecular survey of borrelia burgdorferi in sika deer from Jilin Province in China

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Identification and molecular survey of Borrelia burgdorferi ...

脑脊液中borrelia burgdorferi螺旋体的抗原

中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会

作者： Bintao Zhai Qingli Niu Jifei Yang Zhijie Liu Hong Yin Qiaoying Zeng The College of Veterinary Medicine Gansu Agricultural University State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences

Background: Lyme disease is caused by borrelia burgdorferi. Various animals and wildlife are natural reservoirs of the disease. It is reported that sika deer may as an important reservoir hosts for several species of borrelia burgdorferi and transmit them to domestic humans and animals. However, little is known about the presence of borrelia pathogens in sika deer in China. The prevalence of borrelia spp. in sika deer from Jilin Province in China was assessed. Methods: Seventy-one blood samples of sika deer were collected at four sites from Jilin Province during June and July, 2015, and tested by nested PCR based on the 5s-23 s rRNA, Osp A, Fla and 16 S rRNA genes of the borrelia burgdorferi. The PCR results were confirmed by DNA sequencing based on long fragment of 16 S rRNA gene. The identification of borrelia burdorferi species were determined according to obtained gene sequences of selected positive samples from each sampling area. Results: 12 samples were positive for borrelia spp.(16.9%, 95% CI=10.5-28.8). After sequences alignment, three representative positive sequences from three sites were identified as being infected with borrelia spp. that have high similarities(96.7-99.6%) with B. garinii species of the strain T-MDJ(HM007279) from China. Conclusion: This is the first report of borrelia garinii in sike deer from Jilin Province, in China. The data obtained indicates that sika deer might be a nature host and a potential source spreads this pathogen to domestic animals, even human in China.

关键词： borrelia burgdorferi sika deer identification survey

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

国际神经病学神经外科学杂志 1994年第6期 326-327页

作者：于丽崔子平

可疑神经型莱姆病(Neurologic Lyme Disease,NLD)患者的活动性感染是很难证明的,与大多数神经系统细菌感染不同,很难培养或染色CSF中该病原体。诊断主要依靠临床表现及实验室查到特异性抗体,然而此类抗体只能证实以往接触过包柔氏(Borre... 详细信息

可疑神经型莱姆病(Neurologic Lyme Disease,NLD)患者的活动性感染是很难证明的,与大多数神经系统细菌感染不同,很难培养或染色CSF中该病原体。诊断主要依靠临床表现及实验室查到特异性抗体,然而此类抗体只能证实以往接触过包柔氏(borrelia burgdorferi,BB)螺旋体而非急性感染。从CSF中检测到BB成份是神经系统感染的直接证据。用PCR扩增CSF中BB核酸虽然灵敏,但易被污染和出现假阳性。一种可靠、敏感的酶联免疫吸附试验(ELISA),在神经系统感染数小时内就能证实CSF中BB抗原(BBa)的存在,它对疑有NLD的病人是一项有用的辅助检查。该试验可分成两部

关键词：螺旋体 CSF 夺获抗原 borrelia burgdorferi

Production of reactive oxygen species and expression of inducible nitric oxide synthase in rat isolated Kupffer cells stimulated by Leptospira interrogans and borrelia burgdorfen

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2006年第19期12卷 3077-3081页

作者： Antonella Marangoni Silvia Accardo Rita Aldini Massimo Guardigli Francesca Cavrini Vittorio Sambri Marco Montagnani Aldo Roda Roberto Cevenini Sezione di Microbiologia DMCSS University of Bologna BolognaItaly Istituto di Scienze Chimiche University of Bologna BolognaItaly Dipartimento di Scienze Farmaceutiche University of Bologna BolognaItaly Dipartimento di Medicina Interna e Gastroenterologia University of Bologna BolognaItaly

AIM： To evaluate the production of reactive oxygen species （ROS） and the expression of inducible nitric oxide synthase （iNOS） in rat isolated Kupffer cells （KCs） stimulated by Leptospira interrogans and borrelia burgdorferi. METHODS： Rat Kupffer cells were separated by perfusion of the liver with 0.05% collagenase, and purified by Percoll gradients. Pudfied Kupffer cells were tested in vitro with alive *** and B. burgdorferi preparations. The production of ROS was determined by chemiluminescence, whereas iNOS protein expression was evaluated by Western blot assay using anti-iNOS antibodies. RESULTS： B. burgdorferi and to a less extent L. interrogans induced ROS production with a peak 35 min after infection. The chemiluminescence signal progressively diminished and was undetectable by 180 min of incubation. Leptospirae and borreliae induced an increased iNOS expression in Kupffer cells that peaked at 6 hours and was still evident 22 h after infection. CONCLUSION： Both genera of spirochetes induced ROS and iNOS production in rat Kupffer cells. Since the cause of liver damage both in leptospiral as well as in borrelial infections are still unknown, we suggest that leptospira and borrelia damage of the liver can be initially mediated by oxygen radicals, and is then maintained at least in part by nitric oxide.

关键词： Leptospira interrogans borrelia burgdorferi Inducible nitric oxide synthase Nitric oxide Reactive oxygen species Kupffer cells Chemiluminescence

Seroepidemiological Investigation of Lyme Disease and Human Granulocytic Anaplasmosis among People Living in Forest Areas of Eight Provinces in China

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2013年第3期26卷 185-189页

作者： HAO Qin GENG Zhen HOU Xue Xia TIAN Zhen YANG Xiu Jun JIANG Wei Jia SHI Yan ZHAN Zhi Fei LI Guo Hua YU De Shan WANG Hua Yong XU Jian Guo WAN Kang Lin National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention State Key Laboratory for Infectious Disease Prevention and Control Henan Oil Prospecting Bureau Jilin Provincial Centre for Disease Control and Prevention Guizhou Provincial Centre for Disease Control and Prevention Qinghai Provincial Centre for Disease Control and Prevention Hunan Provincial Centre for Disease Control and Prevention Shanxi Provincial Centre for Disease Control and Prevention Gansu Provincial Centre for Disease Control and Prevention Miyun Centre for Disease Control and Prevention

Objective Lyme disease and Human granulocytic anaplasmosis are tick-borne diseases caused by borrelia burgdorferi and Anoplasrna phagocytophilum respectively. We have investigated infection and co-infection of the two diseases in the population of forest areas of eight provinces in China by measuring seroprevalence of antibodies against B. burgdorferi and A. phagocytophilum. Methods Forest areas in 8 provinces were chosen for investigation using whole sampling and questionnaire survey methods. 3 669 serum samples from people in the forest areas were tested for the presence of antibodies by indirect immunofluorescent assay （IFA）. Results Seroprevalence against B. burgdorferi was 3% to 15% and against A. phagocytophilum was 2% to 18% in the study sites in the 8 provinces in China. We also found co-infection of B. burgdorferi and A. phagocytophilum in 7 of the 8 provinces （the exception being the Miyun area in Beijing）. The seroprevalence for both B. burgdorferi and A. phagocytophilum was significantly higher among people exposed to ticks than among people who were not exposed to ticks. Conclusion We conclude that both pathogens are endemic in the forest areas in the eight provinces, but the prevalence of B. burgdorferi and A. phagocytophilum differs between the provinces.

关键词： borrelia burgdorferi Anaplasma phagocytophilum Co-infection Residents of forest areas

维普期刊数据库

Clinical Evidence of Lyme Disease in Dogs and Disease Awareness Among Students and Veterinarians in Veracruz, Mexico

在线全文

学校读者我要写书评

暂无评论

Veterinary Science Research 2019年第2期1卷 10-17页

作者： Blanca Paola Boria-Gamboa JoséAlfredo Villagómez-Cortés David Itzcoatl MartiInez-Herrera Facultad de Medicina Veterinaria y Zootecnia Universidad VeracruzanaVeracruzMéxico

Background:Lyme disease is a relatively new and zoonotic canine pathology mostly unknown by people involved in the management and care of ***:to assess the knowledge about Lyme disease by veterinarians and veterinary students in Veracruz,***:three questionnaires were designed and applied to 290 individuals(40 small animal veterinarians,50 in other professional practice and 200 veterinary students).Results and discussion:in general,the three groups were unaware about the disease,although there is a high interest in learning about this *** from seven universities included in this study stated that Lyme disease was not part of the veterinary curriculum in their *** clinical cases suggestive of Lyme disease occurring in dogs in the area were detected at the time of this ***:although the presence of the agent causing Lyme disease has not been demonstrated in the studied area due to the lack of laboratory support and the apparent absence of the vector,it cannot be ruled out;furthermore,it is important that veterinary practitioners and student alike be aware of the potential presence of Lyme disease,paying particular attention to differential diagnosis when resembling clinical signs are observed.

关键词： borrelia burgdorferi Curricula Professional communication Survey Tick disease Veterinary education Zoonosis