检索结果-南通市图书馆

Inhibition of <i>Ehrlichia canis</i>and <i>Babesia canis</i>transmission among ticks fed together on dogs vaccinated with Bm86 antigen

引用

Open Journal of Animal Sciences 2013年第3期3卷 24-32页

作者： Alina Rodríguez-Mallon Gervasio H. Bechara Rosangela M. Zacarias Efrain Benavides-Ortiz Jose Luis Soto-Rivas Arlen Patricia Gómez-Ramírez Javier Andrés Jaimes-Olaya Mario Pablo Estrada-García Centro de Ingeniería Genética y Biotecnología Habana Cuba Facultad de Ciencias Agrarias Universidad de Ciencias Comerciales Altamira Nicaragua Facultad de Ciencias Agropecuarias Universidad de La Salle Bogotá DC Colombia Universidade Estadual Paulista-UNESP Jaboticabal Brazil

GAVAC (Heber Biotec S.A, Havana, Cuba) is a commercially available vaccine developed with the Rhipicephalus (Boophilus) microplus Bm86 recombinant antigen. Bm86 is a “concealed” antigen that is present in the plasmatic membrane of tick gut epithelial cells with unknown function so far. It is well known that after vaccination in the last fifteen years in Cuba, there was a significant decrease of babesiosis (Babesia bovis and Babesia bigemina) and anaplasmosis (Anaplasma marginale) in cattle. A reduced transmission capacity of ticks fed on tick-immune animals and humans has been reported for several tick-borne pathogens. Recent experiments have demonstrated that an anti-tick vaccine may contribute to the control of tick-borne pathogens not only by decreasing the exposure of susceptible hosts to ticks, but also by reducing the vector capacity of ticks. In this study, the potential of Bm86 vaccination to interfere with pathogen transmission among ticks was evaluated by using as experimental model the brown dog tick Rhipicephalus sanguineus and the tickborne Babesia canis and Ehrlichia canis pathogens. Dogs, vaccinated and not vaccinated, were infested with pathogen-infected ticks and noninfected nymphs of R. sanguineus. After feeding, the pathogen transmission to newly molted adults from co-feeding uninfected nymphs was studied by conventional PCR and qPCR. Results suggest that the anti-Bm86 antibodies could be able to block the transmission of B. canis and/or E. canis from infected to non-infected ticks.

关键词： Rhipicephalus sanguineus Ehrlichia canis Babesia canis Tick-Borne Diseases Dogs Bm86

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Changes in the Ovarian Polyamine Content and Seed Set Efficiency of Cotton by the Plant Growth Regulator Bm86

引用

American Journal of Plant Sciences 2012年第9期3卷 1328-1335页

作者： Androniki C. Bibi Derrick M. Oosterhuis Evangelos D. Gonias Department of Crop Soil and Environmental Sciences University of Arkansas Fayetteville USA

Naturally occurring polyamines in plants have been implicated in floral initiation, and fruit development. The plant growth regulator Bm86 was formulated to stimulate seed production and fruit growth by enhancing polyamine synthe-sis. The objectives of this study were to determine the effect of Bm86 on free polyamine content in cotton (Gos-sypium hirsutum L.) ovaries and on seed set efficiency, and also to investigate genotypic differences in ovarian polyamine content. Bm86 was applied at the first flower stage and two weeks later at 2.34 mL/ha. This study showed that application of Bm86 had a significant positive effect on ovarian polyamine content of cotton. Putrescine and sper-midine one week after the 1st Bm86 application and putrescine two week after the 1st Bm86 application, were significantly increased compared to the untreated control. Higher seed set efficiency with the Bm86 application was observed when the total number of seeds was used for the calculation. However, when the number of harvestable seeds was used to calculate seed set efficiency Bm86 had no significant effect. In addition, application of Bm86 did not significantly alter seedcotton yield of the crop. Only small differences in ovarian polyamine content were detected among the genotypes tested, possibly due to the narrow genetic pool of the commercial cotton genotypes used. Application of Bm86 can significantly increase cotton seed number by enhancing polyamines biosynthesis, but further research is needed to determine how to capitalize on the increased potential number of harvestable seeds.

关键词： Putrescine Spermidine Spermine Bm86 Gossypium hirusutm L. Ovaries

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

微小牛蜱Bm86基因的克隆及重组表达载体的构建

微小牛蜱Bm86基因的克隆及重组表达载体的构建

引用

中国畜牧兽医学会家畜寄生虫学分会第五次代表大会暨第八次...

作者：李文卉罗建勋党志胜高金亮关贵全马米玲刘志杰刘爱红任巧云孙红武殷宏中国农业科学院兰州兽医研究所

微小牛蜱(Boophilus microplus)在我国分布广泛,不仅可大量吸食动物的血液从而影响宿主的健康与生长性能,更重要的是它可作为牛巴贝斯虫和双芽巴贝斯虫的传播媒介,而这两种病原是我国乃至世界范围牛巴贝斯虫病的罪魁祸首,因此对微小牛... 详细信息

微小牛蜱(Boophilus microplus)在我国分布广泛,不仅可大量吸食动物的血液从而影响宿主的健康与生长性能,更重要的是它可作为牛巴贝斯虫和双芽巴贝斯虫的传播媒介,而这两种病原是我国乃至世界范围牛巴贝斯虫病的罪魁祸首,因此对微小牛蜱进行防制不仅不仅可预防其本身造成的直接危害,更重要的是还可防制牛的巴贝斯虫病。目前,我国对蜱的防治仍主要依靠化学药物,近年来,国外在蜱的免疫防制上进展非常迅速,已形成了以Bm86基因表达产物制备的2种针对微小牛蜱的商品化疫苗,并在实际应用中取得了较好结果。本试验根据国外已发表的Bm86基因序列设计适当的引物,通过RT-PCR 扩增出Bm86基因片段,通过序列测定证实谊序列完全正确,并已构建了重组表达载体pPIC9K-Bm86,目前该项工作仍在进行中。

关键词：基因克隆重组表达载体构建微小牛蜱 Bm86

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：