检索结果-南通市图书馆

Identification of (-)-bornyl diphosphate synthase from blumea balsamifera and its application for (-)-borneol biosynthesis in Saccharomyces cerevisiae

引用

Synthetic and Systems Biotechnology 2022年第1期7卷 490-497页

作者： Rui Ma Ping Su Qing Ma Juan Guo Suiqing Chen Baolong Jin Haiyan Zhang Jinfu Tang Tao Zhou Chenghong Xiao Guanghong Cui Luqi Huang School of Pharmacy Henan University of Chinese MedicineZhengzhouChina State Key Laboratory Breeding Base of Dao-di Herbs National Resource Center for Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijingChina School of Pharmacy Guizhou University of Traditional Chinese MedicineGuiyangChina

Borneol is a precious monoterpenoid with two chiral structures,(-)-borneol and(+)-*** diphosphate synthase is the key enzyme in the borneol biosynthesis ***(+)-bornyl diphosphate synthases have been reported,but no(-)-bornyl diphosphate synthases have been *** balsamifera leaves are rich in borneol,almost all of which is(-)-*** this study,we identified a high-efficiency(-)-bornyl diphosphate synthase(BbTPS3)from *** that converts geranyl diphosphate(GPP)to(-)-bornyl diphosphate,which is then converted to(-)-borneol after dephosphorylation in ***3 exhibited a K m value of 4.93±1.38μM for GPP,and the corresponding k cat value was 1.49 s−*** strategies were applied to obtain a high-yielding(-)-borneol producing yeast strain.A codon-optimized BbTPS3 protein was introduced into the GPP high-yield strain MD,and the resulting MD-B1 strain produced 1.24 mg⋅L^(-1)(-)-*** truncating the N-terminus of BbTPS3 and adding a Kozak sequence,the(-)-borneol yield was further improved by 4-fold to 4.87 mg⋅L^(-1).Moreover,the(-)-borneol yield was improved by expressing the fusion protein module of ERG20 F96W-N127W-YRSQI-t14-BbTPS3K2,resulting in a final yield of 12.68 mg⋅L^(-1) in shake flasks and 148.59 mg⋅L^(-1) in a 5-L *** work is the first reported attempt to produce(-)-borneol by microbial fermentation.

关键词： Metabolic engineering (-)-borneol (-)-bornyl diphosphate synthase blumea balsamifera Saccharomyces cerevisiae

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Microscopic Structural Comparison between Epidermal Trichomes in blumea balsamifera (L.) DC. and blumea laciniata (Roxb.) DC

引用

Journal of Biosciences and Medicines 2016年第12期4卷 70-75页

作者： Xiaolu Chen Yingbo Zhang Yuxin Pang Qian Jiang Tropical Crops Genetic Resources Institute Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China Ministry of Agriculture Danzhou China Hainan Provincial Engineering Research Center for blumea balsamifera Danzhou China

Immature leaves and stems, which were about one to three centimeters nearby the stem tips, of blumea balsamifera (L.) DC. (BB) and blumea laciniata (Roxb.) DC. (BL) were cut into sections under ?18?C with a frozen section machine, and observed under an optical microscope. Results show that the most significant difference is that the BB has only one kind of glandular trichomes, while the BL has two. The glandular trichomes found on BB terms were all short glandular hairs (SGH), which were not longer than 100 μm. On BL stems, besides the SGH, the long glandular hairs (LGH), which were longer than 200 μm, were also found. By the factors pointed out in present study, the BB and BL and be distinguished from each other.

关键词： blumea balsamifera blumea laciniata Sambong Ainaxiang Glandular Hair

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Morphological Development of Sambong (blumea balsamifera (L.) DC.) Leaf Studied by Frozen Section and Thin Section

引用

Journal of Biosciences and Medicines 2016年第4期4卷 10-13页

作者： Liwei Liu Xiaolu Chen Quan Yang Yuxin Pang Tropical Crops Genetic Resources Institute Chinese Academy of Tropical Agricultural Sciences /Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China Ministry of Agriculture Danzhou China Hainan Provincial Engineering Research Center for blumea balsamifera Danzhou China School of Traditional Chinese Medicine Guangdong Pharmaceutical University Guangzhou China

The sambong (blumea balsamifera (L.) DC.) leaf was investigated by applying frozen section and thin section technology, and observed through optical microscope. The results showed that the sambong leaf was firstly originated nearby the apical bud at the stem tip. The first leaf grows fast, that the mitosis happens frequently and the cytoplasm manufacture rapidly. The cells in the first few leaves have not yet differentiated into the spongy parenchyma and palisade parenchyma. In the mature leaf, the spongy parenchyma and palisade parenchyma were fully developed. The epidermal hairs were firstly developed on the abaxial side of the first leaf.

关键词： Sambong blumea balsamifera Ainaxiang Nalong Morphological Development Thin Section

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Effect of Magnesium on Accumulations of Primary Metabolites and Yield of blumea balsamifera

引用

Agricultural Biotechnology 2020年第4期9卷 56-59,67页

作者： Dan WANG Huiping LAN Yingbo ZHANG Xuan HU Xiaolu CHEN Yuxin PANG Fulai YU Tropical Crops Genetic Resources Institute Chinese Academy of Tropical Agricultural Sciences/Hainan Provincial Engineering Research Center for Blumea balsamiferaHaikou 571101China School of Chinese Materia Medica Resources Guangdong Pharmaceutical UniversityYunfu 527500China

[Objectives]This study was conducted to investigate the effects of magnesium on the yield of blumea balsamifera(L.)*** the accumulation of primary metabolites that affect yield of the medicinal material.[Methods]The annual seedlings of *** were selected as experimental *** treatment concentrations of magnesium(Mg)were set as 0,1.5,15 and 150 mg/ml supplied by MgSO4·*** yield of the medicinal material was measured *** the content of total sugar was determined by 3,5-dinitrosalicylic acid colorimetry;the content of crude protein was determined by the Kjeldahl method;the ash content was determined by the high-temperature burning method;the crude fat content was determined with a crude fat instrument;and the crude fiber content was determined by the acid-base washing and weighing method.[Results]Mg significantly increased the yield of *** medicinal material,especially 15 mg/ml *** was found that in September,October and November,1.5 mg/ml and 15 mg/ml Mg significantly increased the contents of primary metabolites including total sugar,ash,crude protein,crude fat and crude fiber,and 150 mg/ml of Mg increased the accumulation of total sugar,ash,crude protein and crude fiber to different degrees,but had certain inhibitory effect on the accumulation of crude *** December,the application of Mg inhibited the accumulation of total sugar,ash and crude protein to different degrees,but significantly promoted the accumulation of crude fat and fiber.[Conclusions]This study provides a theoretical basis for clarifying the effects of different concentrations of magnesium on plant growth.

关键词： Magnesium(Mg) blumea balsamifera Yield Primary metabolites Total sugar Ash Crude protein

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of Calcium Element on Biomass and Effective Constituents Contents in blumea balsamifera in Slow Growth Period of Winter

引用

Agricultural Science & Technology 2016年第2期17卷 358-361,437页

作者：王丹马青松范佐旺李小婷宛骏陈振夏庞玉新中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点开放实验室海南儋州571737 海南省艾纳香工程技术研究中心海南儋州571737

The one-year-old seedlings of blumea balsamifera （L.） DC. were applied with CaCl2.H2O that supplied Ca in slow growth period of winter three times. The heights, ground diameters, leaf lengths, leaf widths and biomasses of B. balsamifera plants were measured. In addition, the relative contents of total flavones in different parts of B. balsamifera were determined by UV spectrophotometry, and the absolute contents of total flavones were calculated. The relative contents of L-borneol in leaves of B. balsamifera were determined by GC, and the absolute contents of L- borneol were calculated. The results showed that calcium element significantly in- creased the biomasses in leaves, stems and roots of B. balsamifera in slow growth period of winter. The leaf biomass of B. balsamifera in the 5 g/L CaCl2-H2O treat- ment group was significantly higher than those in the other three treatment groups. The leaf biomasses of B. balsamifera in the 10 and 15 g/L CaCl2.H2O treatment groups were significantly higher than that in the CK, with 3.03 and 2.65 times, re- spectively. The application of Ca inhibited the accumulation of total flavones relative contents, but significantly increased the total flavones absolute contents in different parts of B. balsamifera. The relative and absolute contents of L-borneol in the 5 g/L CaCl2 .H2O treatment group were 0.22% and 0.22 g, which were increased by 37.50%, 22.22%, 37.50% and 100%, 100%, 450%, respectively compared with those in the 0, 10 and 15 g/L CaCl2.H2O treatment groups. The Ca element could signifi- cantly promote the accumulation of biomasses in leaves, stems and roots, as well as the absolute contents of total flavones and L-borneol in B. balsamifera in slow growth period of winter.

关键词： Calcium (Ca) blumea balsamifera Slow growth period L-borneol Total flavones: Biomass

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：