检索结果-南通市图书馆

Vascular endothelial growth factor-165b protects the blood-retinal barrier from damage after acute high intraocular pressure in rats

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2022年第8期15卷 1231-1239页

作者： Jing Shen Yi Li Min Li Wei-Xian Liu Hong-Liang Sun Quan-Peng Zhang Xi-Nan Yi Department of Ophthalmology First Affiliated Hospital of Hainan Medical UniversityHaikou 570102Hainan ProvinceChina Grade 18 undergraduate of First Clinical College Hainan Medical UniversityHaikou 571199Hainan ProvinceChina Department of Human Anatomy and Neurobiology School of Basic Medical SciencesCentral South UniversityChangsha 410013Hunan ProvinceChina Key Laboratory of Brain Science Research&Transformation in Tropical Environment of Hainan Province Hainan Medical UniversityHaikou 571199Hainan ProvinceChina Anatomy Laboratory of Hainan Medical University Haikou 571199Hainan ProvinceChina

AIM:To elucidate the role of vascular endothelial growth factor-165b(VEGF-165b)in blood-retinal barrier(BRB)injury in the rat acute glaucoma ***:In this study,the rat acute high intraocular pressure(HIOP)model was established before and after intravitreous injection of anti-VEGF-165b *** expression of VEGF-165b and zonula occludens-1(ZO-1)in rat retina was detected by double immunofluorescence staining and Western blotting,and the breakdown of BRB was detected by Evans blue(EB)***:The intact retina of rats expressed VEGF-165b and ZO-1 protein,which were mainly located in the retinal ganglion cell layer and the inner nuclear layer and were both co-expressed with vascular endothelial cell markers *** acute HIOP,the expression of VEGF-165b was up-regulated;the expression of ZO-1 was down-regulated at 12h and then recovered at 3d;EB leakage increased,peaking at *** intravitreous injection of anti-VEGF-165b antibody,the expression of VEGF-165b protein was no significantly changed;and the down-regulation of the expression of ZO-1 was more obvious;EB leakage became more serious,peaking at *** analysis also showed that EB leakage in the peripheral retina was greater than that in the central ***:The endogenous VEGF-165b protein may protect the BRB from acute HIOP by regulating the expression of *** differential destruction of BRB after acute HIOP may be related to the selective loss of retinal ganglion cells.

关键词： vascular endothelial growth factor-165b blood-retinal barrier high intraocular pressure Evans blue zonula occludens-1

Dynamic changes of inducible nitric oxide synthase expression in rat's retina and its role on blood-retinal barrier injury after acute high intraocular pressure

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2022年第7期15卷 1053-1061页

作者： Min Li Ju-Fang Huang Yi Li Jing Shen Lu-Jia Yang Qian Chen Quan-Peng Zhang Xi-Nan Yi College of Basic Medicine and Life Sciences Hainan Medical UniversityKey Laboratory of Tropical Brain Research and Translation of Hainan ProvinceHaikou 571199Hainan ProvinceChina Department of Human Anatomy and Neurobiology School of Basic Medical SciencesCentral South UniversityChangsha 410013Hunan ProvinceChina Grade 18 Clinical Bachelor of Clinical College of Hainan Medical University Haikou 571199Hainan ProvinceChina Department of Ophthalmology the First Affiliated Hospital of Hainan Medical UniversityHaikou 571199Hainan ProvinceChina

AIM: To clarify the role of inducible nitric oxide synthase(i NOS) in blood-retinal barrier(BRB) injury after acute high intraocular pressure(IOP) in ***: Forty-two Sprague-Dawley(SD) rats were randomized into 7 groups [control(Cont), 3, 6, 12, 24, 48, and 72 h, n=6]. Except Cont group, other groups’ retina tissue was obtained at corresponding time points after a model of acute high IOP have been established in rats. The expression of i NOS and tight junction protein zonula occludens(ZO)-1 was detected by Western blotting. Evans blue(EB;3%) was injected into the great saphenous vein to detect the leakage of EB by spectrophotometer. Nine rats were divided into Cont, 6 h, 12 h groups, the expression of i NOS was localized by immunofluorescence. In order to verify the role of i NOS in the damage to BRB, thirty-six rats were randomly divided into 4 groups [Cont, Cont+inhibitor(Inh), 6 h and 6 h+Inh, n=9]. After treatment with the i NOSspecific inhibitor 1400 W, the expression of i NOS and ZO-1 and the leakage of BRB were detected ***: The immunofluorescence results showed that the expression of i NOS was observed in the Cont group and 6 h group, but not in the 12 h group. i NOS was mainly expressed in the retinal nerve fiber layer, ganglion cell layer and inner nuclear layer and that it did not colocalize with the retinal ganglion cell marker Neu N but was co-expressed with the vascular endothelial cell marker CD31. Western blotting showed that in the early period(3 h, 6 h) after acute high IOP, the expression of i NOS was upregulated, then the down-regulation of i NOS were tested in the follow-up timing spots. ZO-1 expression showed a continuous downregulation after 6 h. The quantitative results for EB showed that the amount of EB leakage began to increase at 3 h after acute high IOP. At 6 h, the leakage of EB was lower, but at 12 h, the leakage of EB was highest, after which it gradually recovered but remained higher than that in the Cont group. The

关键词： inducible nitric oxide synthase acute high intraocular pressure blood-retinal barrier ZO-1

RNA interference targeting NOX4 protects visual function in an experimental model of retinal detachment by alleviating blood-retinal barrier damage

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2021年第1期14卷 50-56页

作者： Kai Dong Nan Yang Jie Ding Yuan-Ye Yan Li Lu Yi-Sai Wang Department of Ophthalmology Anhui Provincial HospitalAnhui Medical UniversityHefei 230001Anhui ProvinceChina Department of Ophthalmology the Second People’s Hospital of HefeiHefei 230001Anhui ProvinceChina Department of Ophthalmology Wannan Medical CollegeWuhu 241001Anhui ProvinceChina Department of Ophthalmology Eye Centerthe First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001Anhui ProvinceChina

AIM:To observe the effects of the inhibition of NADPH oxidase 4(NOX4)expression on the retinal vascular barriers and visual function after retinal detachment(RD).METHODS:RD model was established 3 wk after adenoassocianed virus vector *** retinal tissue was harvested 3 d after RD,and the death of retinal vascular endothelial cells and photoreceptors was observed using electron *** NOX4 expression was detected by Western *** microscopy was used to observe a retinal patch that had been perfused with Evans blue.A modified water maze test was used to detect the time required to find the platform on the water *** visual function of the rats was evaluated and reactive oxygen species(ROS)expression was detected by a fluorescence microplate ***:The retinal patch showed that NOX4 interference significantly reduced the destruction of the tight junctions between the retinal endothelium of RD rats and reduced *** blotting showed decreased expression of the NOX4 protein and decreased expression of ROS in retinal tissue;the Morris water maze test results showed that NOX4 interference significantly decreased the escape latency of the ***:NOX4 interference reduces the production of ROS in retinal vascular endothelial cells after experimental RD,thereby protecting the blood-retinal barrier and protecting visual function.

关键词： NOX4 adeno-associated virus retinal detachment blood-retinal barrier reactive oxygen species

Effect of pyridone agent on blood-retinal barrier in diabetic mice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2017年第6期10卷 890-895页

作者： Si-Qi Xiong Hai-Bo Jiang Hui-Zhuo Xu Xiao-Bo Xia Department of Ophthalmology Xiangya Hospital Central South University Changsha 410008 Hunan Province China

AIM： To evaluate the therapeutic effect of fluorofenidone on disrupted blood-retinal barrier in the diabetic mice and uncover its underlying mechanism. METHODS： db/db mice were randomly chosen for treatment with daily doses of fluorofenidone or placebo at 5-week-old, treatment continued until mice reach 24-week- old. Then, expression of transcriptiona factor insulin gene enhancer binding protein-1 （Islet-I） and vascular endothelial growth factor （VEGF） in murine retinas were evaluated. retinal vascular permeability was assessed by examining the level of albumin in db/db murine retinas. Furthermore, the retinal vessel tight junction was estimated by checking the level of occludin in the murine retinal tissues. RESULTS： After occurrence of diabetic retinopthy in db/ db mice, expressions of transcritpional factor Islet-1 was found to be upregulated in db/db murine retinas compared with non-diabetic controls. Similar to expression pattern of Islet-l, VEGF were also demonstrated to be increased in retinas of db/db mice, which was accompanied by increased retinal vascular leakage and decreased tight junction protein level. Systemetic administration of fluorofenidone repaired broken retinal vascular tight junction by restoring occludin expression in db/db retinal tissue. Consequently, retinal vascular premeability were indicated to be reduced by examining the transudative albumin level in diabetic retinal tissues. Both Islet-1 and VEGF expression were inhibited in the retinas of db/db mice after treatment with fluorofenidone. CONCLUSION： Fluorofenidone significantly protectes retinal tight junction and reduces retinal vascular leakage. The phenomenon can be partially attributed to reducing overexpression of Islet-1 and VEGF in diabetic retinal tissues.

关键词： pyridone agent diabetic retinopathy blood-retinal barrier

Upregulated inflammatory associated factors and blood-retinal barrier changes in the retina of type 2diabetes mellitus model

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2016年第11期9卷 1591-1597页

作者： Rui-Jin Ran Xiao-Ying Zheng Li-Ping Du Xue-Dong Zhang Xiao-Li Chen Shen-Yin Zhu Department of Ophthalmology the First Affiliated Hospital of Chongqing Medical University Chongqing Key Laboratory of Ophthalmology Chongqing Eye InstituteChongqing 400016 China University Hospital of Hubei University for Nationalities Enshi 445000 Hubei Province China

AIM： To examine the expression of high mobility group box-1（HMGB-1） and intercellular adhesion molecule-1（ICAM-1） in the retina and the hippocampal tissues; and further to evaluate the association of these two molecules with the alterations of blood-retinal barrier（BRB） and blood-brain barrier（BBB） in a rat model of type 2 ***： The type-2 diabetes mellitus（DM） model was established with a high-fat and high-glucose diet combined with streptozotocin（STZ）. Sixteen weeks after DM induction, morphological changes of retina and hippocampus were observed with hematoxylin-eosin staining, and alternations of BRB and BBB permeability were measured using Evans blue method. Levels of HMGB-1 and ICAM-1 in retina and hippocampus were detected by Western blot. Serum HMGB-1 levels were determined by enzyme-linked immunosorbent assay（ELISA）.RESULTS： A significantly higher serum fasting blood glucose level in DM rats was observed 2wk after STZ injection（P 〈0.01）. The serum levels of fasting insulin,Insulin resistance homeostatic model assessment（IRHOMA）,total cholesterol（TC）, total triglycerides（TG） and low density lipoprotein cholesterol（LDL-C） in the DM rats significantly higher than those in the controls（all P 〈0.01）.HMGB-1（0.96±0.03, P 〈0.01） and ICAM-1（0.76±0.12, P 〈0.05） levels in the retina in the DM rats were significantly higher than those in the controls. HMGB-1（0.83±0.13, P 〈0.01） and ICAM-1（1.15 ±0.08, P 〈0.01） levels in the hippocampal tissues in the DM rats were alsosignificantly higher than those in the controls. Sixteen weeks after induction of DM, the BRB permeability to albumin-bound Evans blue dye in the DM rats was significantly higher than that in the controls（P 〈0.01）.However, there was no difference of BBB permeability between the DM rats and controls. When compared to the controls, hematoxylin and eosin staining showed obvious irregularities in the DM ***： BRB permeability increases significantly in rats with type-2 DM, which may be as

关键词： blood-retinal barrier blood-brain barrier diabetes mellitus permeability: high mobility group box-1protein rats

The development of blood-retinal barrier during the interaction of astrocytes with vascular wall cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2014年第10期9卷 1047-1054页

作者： Huanling Yao Tianshi Wang Jiexin Deng Ding Liu Xiaofei Li Jinbo Deng Institute of Neurobiology College of Life ScienceHenan University

Astrocytes are intimately involved in the formation and development of retinal vessels. Astrocyte dysfunction is a major cause of blood-retinal barrier injury and other retinal vascular diseases. In this study, the development of the retinal vascular system and the formation of the blood-ret-inal barrier in mice were investigated using immunolfuorescence staining, gelatin-ink perfusion, and transmission electron microscopy. The results showed that the retinal vascular system of mice develops from the optic disc after birth, and radiates out gradually to cover the entire retina, taking the papilla optica as the center. First, the superifcial vasculature is formed on the inner retinal layer;then, the vasculature extends into the inner and outer edges of the retinal inner nuclear layer, forming the deep vasculature that is parallel to the superifcial vasculature. The blood-retinal barrier is mainly composed of endothelium, basal lamina and the end-feet of astrocytes, which become mature during mouse development. Initially, the naive endothelial cells were immature with few organelles and many microvilli. The basal lamina was uniform in thickness, and the glial end-feet surrounded the outer basal lamina incompletely. In the end, the blood-retinal barrier matures with smooth endothelia connected through tight junctions, rela-tively thin and even basal lamina, and relatively thin glial cell end-feet. These ifndings indicate that the development of the vasculature in the retina follows the rules of“center to periphery”and“superifcial layer to deep layers”. Its development and maturation are spatially and tempo-rally consistent with the functional performance of retinal neurons and photosensitivity. The blood-retinal barrier gradually becomes mature via the process of interactions between astro-cytes and blood vessel cells.

关键词： nerve regeneration retina growth development blood vessels blood-retinal barrier astrocytes immunofluorescence ultrastructure mouse collagen IV glial fibrillary acidic protein NSFC grant neural regeneration

Lycium barbarum polysaccharides related RAGE and Aβ levels in the retina of mice with acute ocular hypertension and promote maintenance of blood retinal barrier

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2020年第12期15卷 2344-2352页

作者： Xue-Song Mi Qian Feng Amy Cheuk Yin Lo Raymond Chuen-Chung Chang Sookja Kim Chung Kwok-Fai So Department of Ophthalmology the First Affiliated Hospital of Jinan UniversityGuangzhouGuangdong ProvinceChina Guangdong-Hong Kong-Macao Institute of CNS Regeneration Jinan UniversityGuangzhouGuangdong ProvinceChina Changsha Academician Expert Workstation Aier Eye Hospital GroupChangshaHunan ProvinceChina State Key Laboratory of Brain and Cognitive Sciences Hong Kong Special Administrative RegionChina Department of Ophthalmology The University of Hong KongHong Kong Special Administrative RegionChina Faculty of Medicine Macao University of Science and TechnologyMacao Special Administrative RegionChina School of Biomedical Science LKS Faculty of MedicineThe University of Hong KongHong Kong Special Administrative RegionChina

Our previous study verified the protective effects of Lycium barbarum polysaccharides(LBP)on retinal neurons and blood vessels in acute ocular hypertension(AOH)*** investigate the effect of LBP on the reactivity of retinal glial cells,an AOH mouse model was established in one eye by maintaining ocular hypertension of 90 mm Hg for 60 *** LBP solution(1 mg/kg)or phosphate-buffered saline was administrated to the mice by gavage daily,starting 7 days before the AOH insult and continuing until the mice were sacrificed for specimen collection on day 4 *** AOH insult,increased numbers of astrocytes and microglia were observed,together with decreased expression of the following glial cell biomarkers in the retinal ganglion cells of AOH mice:glial fibrillary acidic protein,glutamine synthetase,aquaporin-4,S-100 proteins,ionized calcium-binding adaptor molecule 1,amyloid precursor protein and receptor of advanced glycosylation *** intervention with LBP,the above changes were significantly ***,morphological remodeling of blood vessel-associated retinal astrocytes,marked by glial fibrillary acidic protein,was also *** results,taken together,suggest that LBP regulated the production of amyloid-βand expression of receptor of advanced glycosylation end-products,as well as mediating the activity of retinal glial cells,which may lead to the promotion of better maintenance of the blood-retinal barrier and improved neuronal survival in AOH *** study was approved by the Committee for the Use of Live Animals in Teaching and Research(approval ***-#1664-08).

关键词： astrocyte blood-retinal barrier glial cell Lycium barbarum microglia model plasticity remodel retina

Infl iximab relieves blood retinal barrier breakdown through the p38 MAPK pathway in a diabetic rat model

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2017年第12期10卷 1824-1829页

作者： Mao-Song Xie Yong-Zheng Zheng Li-Bin Huang Guo-Xing Xu Department of Ophthalm ology First Afi liated Hospital of Fujian Medical UniversityFuzhou 350005Fujian ProvinceChina Department of Ophthalmology Affiliated People’s Hospital of Fujian University of Traditional Chinese MedicineFuzhou 350005Fujian ProvinceChina

AIM： To clarify the mechanism of infliximab treatment in diabetic macular edema （DME） and to provide a new alternative therapy for DME. METHODS： Rats were randomly divided into the control group, the model group and the infliximab treatment group. A diabetic rat model was created. The concentration of TNF-α in the vitreous body was detected by ELISA. The expressions of B-Raf, p38, claudin-1 and occludin in the retina were detected by Western blot. The integrity of the blood retinal barrier （BRB） was measured using Evan＇s blue as a tracer. RESULTS： After three months and six months of the diabetes model, the vitreous TNF-α level in the model group was higher than that of the control group. It was also higher in treated group than that of the control group but was lower than that of the model group. The differences among the three groups were statistically significant （at 3mo, F=857.098, P〈0.001; 6mo, F=1261.897, P〈0.001）. The retina B-Raf and p38 levels in the model group were higher than that of the control group. They were also higher in treated group than that of the control group but were lower than that of the model group. The differences among the three groups were statistically significant （B-Raf at 3mo, F=106.596, P〈0.001 and at 6mo, F=200.681, P〈0.001; p38 at 3mo, F=41.662, P〈0.001 and at 6mo, F=67.979, P〈0.001）. The retina claudin-1 and occludin levels in the model group were lower than that of the control group. They were also lower in treated group than that of the control group but were higher than that of the model group. The differences among three groups were statistically significant （claudin-1 at3mo, F=-139.088, P〈0.001 and at 6mo, F=128.415, P〈0.001; occludin at 3mo, F=-92.733, P〈0.001 and at 6mo, F--104.478, P〈0.001）. The retinal Evans blue leakage in the model group was higher than that of the control group. It was also higher in treated group than that of the control group but was lower than that of the model group. The differences among the t

关键词： tumor necrosis factor-α blood-retinal barrier diabetic macular edema infliximab pathogenesis

Preventive effect of chrysin on experimental autoimmune uveitis triggered by injection of human IRBP peptide 1-20 in mice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2017年第8期14卷 702-711页

作者： Xiangda Meng Sijie Fang Zhuhong Zhang Yang Wang Caiyun You Jingkai Zhang Hua Yan Department of Ophthalmology Tianjin Medical University General HospitalTianjin 300052China

Uveitis is a common cause of blindness *** autoimmune uveitis(EAU)is an animal model of noninfectious ***(5,7-dihydroxyflavone)is a member of the flavonoid family and has anti-inflammatory *** immunized C57BL/6J mice with human interphotoreceptor retinoid-binding protein peptide 1–20 to induce *** was administered intragastrically at 25 mg/kg daily to the chrysin-treated mice from 3 days before immunization to 21 days after *** was administered to the mice in the control group according to the same *** clinical and histopathological scores,increased integrity of the blood–retinal barrier(BRB)and higher expression of tight junction proteins were observed in the chrysin-treated *** significantly decreased the proportions of Th1,Th17 and CD4^(+)CD3^(+)CD62L^(+)Th0 cells,and increased the proportion of Treg *** macrophage infiltration and the expression of inducible nitric oxide synthase in the retina were efficiently inhibited by chrysin *** chrysin-treated mice,the expression of interferon-γ,interleukin(IL)-17A,IL-6,IL-1βand tumor necrosis factor-αwas reduced in the retina,whereas higher levels of transforming growth factor-βwere ***,NF-κBp65 was downregulated after chrysin *** conclusion,as an anti-inflammatory molecule,chrysin exerts a preventive effect on EAU by modulating the balance among helper T-cell subsets and suppressing ocular inflammation,thereby maintaining the integrity of the BRB.

关键词： blood-retinal barrier chrysin experimental autoimmune uveitis NF-κB T helper-cell subsets