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Development of elite restoring lines by integrating blast resistance and low amylose content using MAS

Journal of Integrative Agriculture 2018年第1期17卷 16-27页

作者： XIAO Wu-ming PENG Xin LUO Li-xin LIANG Ke-qin WANG Jia-feng HUANG Ming LIU Yong-zhu GUO Tao LUO Wen-long YANG Qi-yun ZHU Xiao-yuan WANG Hui CHEN Zhi-qiang National Engineering Research Center of Plant Space Breeding South China Agricultural University Plant Protection Research Institute Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of High Technology for Plant Protection

blast resistance and grain quality are major problems in hybrid rice production in China. In this study, two resistance （R） genes, Pi46 and Pita, along with the gene Wxb, which mainly affects rice endosperm amylose content （AC）, were introgressed into an elite indica restoring line, R8166, which has little blast resistance and poor grain quality through marker-assisted selection （MAS）. Eight improved lines were found to have recurrent genome recovery ratios ranging from 88.68 to 96.23%. Two improved lines, R163 and R167, were selected for subsequent studies. R167, which has the highest recovery ratio （96.23%）, showed no significant differences in multiple agronomic traits. In contrast, R163 with the lowest recovery ratio （88.68%） exhibited significant differences in heading date and yield per plant compared with the recurrent parent. At two developmental stages, R163 and R167 had greatly enhanced resistance to blast over the recurrent parent. Similar trends were also observed for agronomic traits and blast resistance in R163- and R167-derived hybrids when compared with the counterparts from R8166. In addition, R163, R167, and their derived hybrids significantly improved the grain quality traits, including amylose content （AC）, gel consistency （GC）, chalky grain rate （CGR）, and degree of endosperm chalkiness （DEC）. It confirmed the success of efficiently developing elite restoring lines using MAS in this study.

关键词： rice restoring line blast resistance grain quality MAS

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Improvement of Upland Rice Variety by Pyramiding Drought Tolerance QTL with Two Major blast resistance Genes for Sustainable Rice Production

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Rice science 2021年第5期28卷 493-500,I0033-I0036页

作者： Vishalakshi Balija Umakanth Bangale Senguttuvel Ponnuvel Kalyani Makarand Barbadikar Srinivas Prasad Madamshetty Sanjeeva Rao Durbha Hari Yadla Sheshu Madhav Maganti Biotechnology Division ICAR-Indian Institute of Rice ResearchRajendra NagarHyderabad 500030India Hybrid Rice Division ICAR-Indian Institute of Rice ResearchRajendra NagarHyderabad 500030India Plant Pathology Division ICAR-Indian Institute of Rice ResearchRajendra NagarHyderabad 500030India Plant Physiology Division ICAR-Indian Institute of Rice ResearchRajendra NagarHyderabad 500030India Regional Agricultural Research Station Professor Jayashankar Telangana State Agricultural UniversityWarangal 506006India Department of Biotechnology Acharya Nagajna UniversityGuntur 522510India

Varalu is an early maturing rice variety widely grown in the rainfed ecosystem preferred for its grain type and cooking ***,the yield of Varalu is substantially low since it is being affected by reproductive drought stress along with the blast *** genetic improvement of Varalu was done by introgressing a major yield QTL,qDTY_(12.1),along with two major blast resistance genes ***54 and Pi1 through marker-assisted backcross *** traits were transferred till BC_(2) generation and intercrossing was followed to pyramid the two *** foreground selection was carried out using linked markers as well as peak markers(RM28099,RM28130,RM511 and RM28163)for the targeted QTL(qDTY_(12.1)),RM206 for Pi54 and RM224 for *** background selection was done using genome-wide SSR *** best lines(MSM-36,MSM-49,MSM-53,MSM-57,MSM-60 and MSM-63)having qDTY_(12.1) and two blast resistance genes in homozygous condition with recurrent parent genome of 95.0%-96.5% having minimal linkage drag of about 0.1 to 0.7 Mb were *** lines showed yield advantage under drought stress as well as irrigated ***-36 showed better performance in the national coordinated trials conducted across India,which indicated that improved lines of Varalu expected to replace Varalu and may have an important role in sustaining rice *** present study demonstrated the successful marker-assisted pyramiding strategy for introgression of genes/QTLs conferring biotic stress resistance and yield under abiotic stress in rice.

关键词： blast resistance drought stress marker-assisted gene pyramiding rainfed ecosystem upland rice

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Transposon-Mediated NLR Exile to the Pollen Allows Rice blast resistance without Yield Penalty

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Molecular Plant 2017年第5期10卷 665-667页

作者： Stella Cesari Thomas Kroj INRA Biology and Genetics of Plant-Pathogen Interactions Campus International de Baillarguet 34398 Montpellier France CIRAD Biology and Genetics of Plant-Pathogen Interactions Campus International de Baillarguet 34398 Montpellier France Supagro Biology and Genetics of Plant-Pathogen Interactions Campus International de Baillarguet 34398 Montpellier France

INTRODUCTIONDisease resistance （R） genes are of major importance in crop pro-tection and breeding as they prevent potentially dramatic yield loss by plant pathogens. Most R genes encode immune recep- tors of the nucleotide-binding and leucine-rich repeat （LRR） domain protein （NLR） family （Jones et al., 2016）. They act inside plant cells by recognizing virulence factors called effectors that pathogens introduce into host cells to promote disease.

关键词： Transposon-Mediated NLR Exile Pollen Allows Rice blast resistance

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Improving blast resistance of a Thermo-Sensitive Genic Male Sterile Rice Line GD-8S by Molecular Marker-Assisted Selection

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Rice science 2008年第3期15卷 179-185页

作者： LIU Wu-ge JIN Su-juan ZHU Xiao-yuan WANG Feng LI Jin-hua LIU Zhen-rong LIAO Yi-long ZHU Man-shan HUANG Hui-jun LIU Yi-bai Rice Research Institute Guangdong Academy of Agricultural Sciences Guangzhou 510640 China Agronomy College Jiangxi Agricultural University Nanchang 330045 China Plant Protection Research Institute Guangdong Academy of Agricultural Sciences Guangzhou 510640 China

The broad-spectrum blast resistance gene Pi-1, from donor line BL122, was introduced into a thermo-sensitive genic male sterile rice line GD-8S, which possessed good grain quality but high susceptibility to rice blast, by using backcross breeding and molecular marker-assisted selection. Five elite improved male sterile lines, RGD8S-1, RGD8S-2, RGD8S-3, RGD8S-4 and RGD8S-5, were selected based on the results of molecular marker analysis, spikelet sterility, recovery rate of genetic background and agronomic traits. Thirty-three representative blast isolates collected from Guangdong Province, China were used to inoculate the improved lines and the original line GD-8S artificially. The resistance frequencies of the improved lines ranged from 76.47% to 100%, much higher than that of the original line GD-8S （9.09%）. On the agronomic characters, there were no significant differences between the improved lines and GD-8S except for flag leaf length and panicle number per plant. The improved lines could be used for breeding hybrid rice with high blast resistance.

关键词： rice blast resistance thermo-sensitive genic male sterile line molecular marker-assisted selection breeding

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Avoidance of Linkage Drag Between blast resistance Gene and the QTL Conditioning Spikelet Fertility Based on Genotype Selection Against Heading Date in Rice

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Rice science 2009年第1期16卷 21-26页

作者： LIU Wen-qiang FAN Ye-yang CHEN Jie SHI Yong-feng Wu Jian-li Chinese National Center for Rice Improvement/State Key Laboratory for Rice Biology China National Rice Research Institute Hangzhou 310006 China

Previous study showed that a linkage drag between a blast resistance gene Pi25（t） and QTLs conditioning spikelet fertility （qSF-6） and number of filled grains per panicle （qNFGP-6） was detected on the short arm of chromosome 6. A larger population was used for further verification, and the results confirmed the linkage drag between the blast resistance gene and QTL conditioning spikelet fertility, other than QTL conditioning number of filled grains per panicle. Breakdown or avoidance of the linkage drag could be achieved by selection against the genotype background of a heading-date gene （qHD-7） that resided in the region between RM2 and RM214 on chromosome 7. For further validation, two lines with almost identical genotypes on all chromosomal regions except the Pi25（t） region on chromosome 6 were chosen to develop a new population The results showed that qSF-6 could be further subdivided into qSF-6-1 and qSF-6-2. When the genotype of the region between RM2 and RM214 was from rice variety Zhong 156, the linkage drag between Pi25（t） and qSF-6-2 was detected and the allele of qSF-6-2 from rice variety Gumei 2 reduced the spikelet fertility. When the genotype of the region between RM2 and RM214 was from Gumei 2, no linkage drag was detected. This indicates that the linkage drag between the blast resistance gene and the QTL conditioning spikelet fertility could be broken down or avoided under a certain background genotype selection against heading-date and provides a marker aided solution for high level of blast resistance and yield breeding in rice and other crops as well.

关键词： rice (Oryza sativa) linkage drag blast resistance quantitative trait locus spikelet fertility heading date

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Relationship Between resistance Gene Analogue and blast resistance in Rice

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Rice science 2009年第2期16卷 99-105页

作者： CHEN Yu-min FAN Cheng-ming YANG Yan HE Yue-qiu Key Laboratory of Agricultural Biodiversity for Pest Management of China Education Ministry Yunnan Agricultural University Kunming 650201 China College of Agronomy and Biotechnology Yunnan Agricultural University Kunming 650201 China Quality Standard and Testing Technology Institute Yunnan Academy of Agricultural Sciences Kunming 650223 China Institute of Agricultural Economy ＆ Information Yunnan Academy of Agricultural Sciences Kunming 650205 China

DNA fragments of 43 rice varieties were amplified with 11 pairs of primers designed based on resistance gene analogue （RGA） of plants, and the blast resistance of the varieties was identified by inoculation with 33 isolates of Magnaporthe grisea collected from Yunnan Province, China. Clustering results revealed a significant correlation between the blast resistance and DNA bands with a correlation coefficient of 0.6117 （α=0.01）, indicating that the resistance analysis based on RGA-PCR clustering analysis coincided with that based on inoculation. The correlation coefficients, ranging from 0.1701 to 0.535, however, depended on the primers. Five pairs of primers, S1/AS3, S1 INV/S2 INV, XLRR For/XLRR Rev, Pto-Kinl IN/Pto-Kin2 IN, and NLRR For/NLRR Rev might be applied for blast resistance identification in consideration of their band numbers and polymorphisms, and their correlation coefficients with blast resistance were 0.5305, 0.4898, 0.4059, 0.3719 and 0.3524, respectively. Besides, indica and japonica rice except two highly susceptible varieties, CO39 and Lijiangxintuanheigu could be well classified by the 11 pairs of primers.

关键词： rice resistance gene analogue blast resistance correlation primer inoculation identification

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Clustering of Major Genes Conferring blast resistance in a Durable resistance Rice Cultivar Gumei 2

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Rice science 2004年第4期11卷 161-164页

作者： WuJian-li CHAIRong-yao FANYe-yang LIDe-bao ZHENGKang-le HeiLEUNG ZHUANGJie-yun StateKeyLaboratoryofRiceBiology ChineseNationalCenterforRiceImprovementChinaNationalRiceResearchInstituteHangzhou310006China／／InstituteofBiotechnologyZhejiangUniversityHangzhou310029China PlantProtectionInstitute ZhejiangAcademyofAgriculturalSciencesHangzhou310021China StateKeyLaboratoryofRiceBiology ChineseNationalCenterforRiceImprovementChinaNationalRiceResearchInstituteHangzhou310006China InstituteofBiotechnology ZhejiangUniversityHangzhou310029China EntomologyandPlantPathologyDivision InternationalRiceResearchInstituteDAPOP.O.Box7777.MetroManilaPhilippines

By using 304 recombinant inbred lines derived from indica rice cross Zhong 156/Gumei 2, a linkage map consisting of 177 marker loci and covering 12 rice chromosomes was constructed and employed for mapping genes conferring blast resistance in rice. Genomic location of gene Pi25(t) conferring neck blast resistance to the Chinese isolate 92-183 (race ZC15) was verified to be located between markers A7 and RG456 on chromosome 6, with genetic distances of 1.7 cM and 1.5 cM to A7 and RG456, respectively. Leaf blast resistance of Gumei 2 to the Philippine isolate Ca89 (lineage 4) was found to be controlled by a single gene. The gene tentatively designated as Pi26(\) was located between makers B10 and R674 on chromosome 6, with genetic distances of 5.7 cM and 25.8 cM to B10 and R674 respectively. Resistant alleles at both gene loci were derived from Gumei 2, indicating an existence of resistance gene cluster in Gumei 2.

关键词： blast resistance molecular mapping gene cluster rice

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Tagging blast resistance Gene Pi 1 in Rice (Oryza sativa) Using Candidate resistance Genes

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Rice Science 2004年第Z1期 251-254页

作者： Menchu BERNADO Entomology and Plant Pathology Division International Rice Research Institute DAPO P.O. Box 7777.Metro Manila Philippines

An F3 population derived from C101LAC/CO39 containing 90 lines was analyzed for blast resistance with 48 candidate genes developed from resistance gene analogs （RGA） and suppression subtractive library. Genetic analysis confirmed that blast resistance of the population was controlled by a single gene Pi 1. One of the candidate genes, R10 was identified as associated with the blast resistance gene on the long arm of chromosome 11 and mapped using a DH population derived from Azucena/IR64. A pair of PCR based primers was designed based on the sequence of R10 for marker-aided selection of the blast resistance gene. The recombination frequency between Pi 1 and the marker was estimated as 1.28%. It suggested that strategy of employing candidate genes is useful for gene identification and mapping. A new RFLP marker and the corresponding PCR marker for tagging of Pi 1 were provided.

关键词： rice candidate gene marker-aided selection blast resistance

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Identification of blast-resistance loci through genome-wide association analysis in foxtail millet(Setaria italica (L.)Beauv.)

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Journal of Integrative Agriculture 2021年第8期20卷 2056-2064页

作者： LI Zhi-jiang JIA Guan-qing LI Xiang-yu LI Yi-chu ZHI Hui TANG Sha MA Jin-feng ZHANG Shuo LI Yan-dong SHANG Zhong-lin DIAO Xian-min College of Life Sciences Hebei Normal UniversityShijiazhuang 050024P.R.China Institute of Crop Breeding Heilongjiang Academy of Agricultural SciencesHarbin 150086P.R.China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081P.R.China Institute of Plant Protection Heilongjiang Academy of Agricultural SciencesHarbin 150086P.R.China

blast disease caused by the fungus Magnaporthe grisea results in significant yield losses of cereal crops across the *** date,very few regulatory genes contributing to blast resistance in grass species have been identified and the genetic basis of blast resistance in cereals remains ***,a core collection of foxtail millet(Setaria italica)containing 888 accessions was evaluated through inoculation with the blast strain HN-1 and a genome-wide association study(GWAS)was performed to detect regulators responsible for blast disease resistance in foxtail *** phenotypic variation of foxtail millet accessions inoculated with the blast strain HN-1 indicated that less than 1.60% of the samples were highly resistant,35.25% were moderately resistant,57.09% were moderately susceptible,and 6.08% were highly *** geographical pattern of blast-resistant samples revealed that a high proportion of resistant accessions were located in lower latitude regions where the foxtail millet growing season has higher rain *** 720000 SNP markers covering the Setaria genome,GWAS showed that two genomic loci from chromosomes 2 and 9 were significantly associated with blast disease resistance in foxtail ***,eight putative genes were identified using rice blast-related transcriptomic *** results of this work lay a foundation for the foxtail millet blast resistance biology and provide guidance for breeding practices in this promising crop species and other cereals.

关键词： foxtail millet blast resistance core collection GWAS

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Improving the resistance of the rice PTGMS line Feng39S by pyramiding blast,bacterial blight,and brown planthopper resistance genes

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The Crop Journal 2022年第4期10卷 1187-1197页

作者： Dabing Yang Lizhong Xiong Tongmin Mou Jiaming Mi National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan 430070HubeiChina

Knowledge of rice(Oryza sativa L.)genes and various DNA markers can be used in genomic breeding programs aimed at developing improved elite rice *** used an efficient genomic breeding approach to pyramid four resistance genes(Pi2,Xa23,Bph14,and Bph15)in the popular photoperiod-and thermosensitive genic male sterile(PTGMS)rice line *** performed foreground selection for the target genes,followed by recombinant selection and background *** process reduced the sizes of the genomic segments harboring the target genes(566.8 kb for Pi2,1143.9 kb for Xa23,774.7 kb for Bph14,and 1574.9 kb for Bph15)and accelerated the recovery of the recurrent parent genome to proportions ranging from 98.77%to 99.16%,thus resulting in four near-isogenic *** assemble the four resistance genes in Feng39S,we performed a double-way cross combined with foreground and background selection to generate two improved lines of Feng39S(Pi2+Xa23+Bph14+Bph15)with a recurrent parent genome recovery of 98.98%.The two lines showed agronomic performance,grain quality,and fertility–sterility transition characteristics similar to those of the original Feng39S *** newly developed PTGMS lines and corresponding hybrid combinations were resistant to various field blast isolates and seven representative isolates of bacterial *** the seedling stage,the lines also showed resistance against brown *** study provides an efficient and accurate genomic breeding approach for introducing desirable traits into PTGMS lines.

关键词： Bacterial blight resistance blast resistance Brown planthopper resistance Genomic breeding PTGMS line Two-line hybrid rice

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