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Chinese Chemical Letters 2019年第3期30卷 545-548页

作者： Keying Bi Rui Tan Ruiting Hao Lanxi Miao Yanqi He Xianghua Wu Junfeng Zhang Rui Xu College of Chemistry and Chemical Engineering Yunnan Normal University College of Energy and Environment Science Yunnan Normal University College of Chemical Science and Technology Yunnan University

bisulfite(HSO_3) is an important sulfur dioxide(SO_2) derivative, which plays a major role in many physiological processes and is also closely associated with a variety of diseases. Thus the development of highly selective and sensitive fluorescent probes is essential to detect HSO_3 in living cells. In this work,we report the synthesis and analysis of a ratiometric fluorescent probe for selective detection of HSO_3 based on the 1,4-nucleophilic addition reaction with the carbazole as an electron donor(D) and aldehyde group as an electron acceptor(A). The addition of HSO_3 and other ions to our probe can be observed by UV vis and fluorescence spectrometry. Our investigation proved that the probe is highly selective and sensitive for HSO_3 and ratiometric changes. Moreover, the probe has good cell permeability and was successfully applied to the detection of exogenous HSO_3 in Hela cells and C. elegans.

关键词： bisulfite Fluorescent probe Ratiometric Colorimetric Fluorescence imaging

The effectiveness of bisulfite-activated permanganate technology to enhance the coagulation efficiency of Microcystis aeruginosa

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Chinese Chemical Letters 2020年第6期31卷 1545-1549页

作者： Yating Zhu Jinfeng Ling Lei Li Xiaohong Guan State Key Laboratory of Pollution Control and Resources Reuse College of Environmental Science and EngineeringTongji UniversityShanghai 200092China Shanghai Institute of Pollution Control and Ecological Security Shanghai 200092China International Joint Research Center for Sustainable Urban Water System Tongji UniversityShanghai 200092China

The effects of bisulfite-activated permanganate technology(PM/BS)as a pre-oxidation process on enhancing Microcystis aeruginosa(***)removal by post coagulation were *** results demonstrated that pretreatment with PM/BS process effectively promoted the algae removal by coagulation with Al2(SO4)3 as the coagulant and this phenomenon was more obvious with the increase of water *** to the sole coagulation,PM/BS pre-oxidation combing with coagulation could neutralize the zeta potential of algal cells effectively,decrease the algal cell size,and lead to the formation of more compact flocs due to the in-situ generated *** effect of oxidant dosages on algal organic matter(AOM)was also studied and no obvious release of macromolecular substances was observed with the dosage of KMn04 increasing from 3.0 mg/L to 7.0 mg/L,suggesting the integrity of algal cells at a high KMn04 ***,PM/BS pre-oxidation could lead to the decrease of most analyzed disinfection by-products(DBPs)at a Al2(SO4)3 dosage of 40.0 mg/*** algae removal efficiency was also significantly enhanced by PM/BS pre-oxidation in the test using real algae-laden *** study indicated that PM/BS process might be a potential assistant technology for algae removal by subsequent coagulation.

关键词： Microcystis aeruginosa Permanganate bisulfite Pre-oxidation Coagulation

The bisulfite genomic sequencing protocol

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Advances in Lung Cancer 2013年第1期2卷 21-25页

作者： Jane J. Pappas André Toulouse Walter Edward Clarke Bradley Department of Anatomy and Neuroscience University College Cork Cork Ireland Department of Pharmacology and Therapeutics McGill University Montreal Canada

The bisulfite genomic sequencing (BGS) protocol has gained worldwide popularity as the method of choice for analyzing DNA methylation. It is this popular because it is a powerful protocol and it may be coupled with many other applications. However, users often run into a slew ofproblems, including incomplete conversion,overly degraded DNA, sub-optimal PCR amplifications, false positives, uninformative results, or altogether failed experiments. We pinpoint the reasons why these problems arise and carefully explain the critical steps toward accomplishing a successful experiment step-by-step. This protocol has worked successfully (>99.9% conversion) on as little as 100 ng of DNA derived from nearly 10-year-old DNA samples extracted from whole blood stored at -80°C and resulted in enough converted DNA for more than 50 PCRreactions. The aim of this article is to makelearning and usage of BGS easier, more efficient and standardized for all users.

关键词： bisulfite Genomic Sequencing DNA Methylation Epigenetics Cancer

Efficient degradation of orange II by ZnMn2O4 in a novel photo-chemical catalysis system

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Frontiers of Chemical Science and Engineering 2020年第6期14卷 956-966页

作者： Qingzhuo Ni Hao Cheng Jianfeng Ma Yong Kong Sridhar Komarneni School of Environmental and Safety Engineering Changzhou UniversityChangzhou 213164China Guangxi Key Laboratory of Green Processing of Sugar Resources College of Biological and Chemical EngineeringGuangxi Science University of and TechnologyLiuzhou 545006China Jiangsu Key Laboratory of Oil-Gas Storage and Transportation Technology Changzhou UniversityChangzhou 213164China Department of Ecosystem Science and Management and Materials Research Institute The Pennsylvania State UniversityUniversity ParkPA 16802USA

A ZnMn2O4 catalyst has been synthesized via a sucrose-aided combustion method and characterized by various analytical *** is composed of numerous nanoparticles(15-110 nm)assembled into a porous structure with a specific surface area(SSA)o f 19.1 m^2.g^-*** catalytic activity has been investigated for the degradation of orange II dye using three different systems,i.e.,the photocatalysis system with visible light,the chemocatalysis system with bisulfite,and the photochemical catalysis system with both visible light and *** last system exhibits the maximum degradation efficiency of 90%,much higher than the photocatalysis system(15%)and the chemocatalysis system(67%).The recycling experiments indicate that the ZnMn204 catalyst has high stability and reusability and is thus a green and eximious ***,the potential degradation mechanisms applicable to the three systems are discussed with relevant theoretical analysis and scavenging experiments for *** active species such as Mn(III),o2^-,H^+,eaq,So4^- and Ho^-are proposed to be responsible for the excellent degradation results in the photo-chemical catalysis system with the ZnMn2o4 catalyst.

关键词： ZnMn2O4 photo-chemical catalysis bisulfite dye degradation

MGMT is down-regulated independently of promoter DNA methylation in rats with all-trans retinoic acidinduced spina bifida aperta

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Neural Regeneration Research 2019年第2期14卷 361-368页

作者： He-Nan Zhang Yi Guo Wei Ma Jia Xue Wei-Lin Wang Zheng-Wei Yuan Key Laboratory of Health Ministry for Congenital Malformation Shengjing Hospital China Medical University Department of Pediatric Surgery Shengjing Hospital China Medical University

O6-methylguanine DNA methyltransferase(MGMT), a DNA repair enzyme, has been reported in some congenital malformations, but it is less frequently reported in neural tube defects. This study investigated MGMT mRNA expression and methylation levels in the early embryo and in different embryonic stages, as well as the relationship between MGMT and neural tube defects. Spina bifida aperta was induced in rats by a single intragastric administration of all-trans retinoic acid on embryonic day(E) 10, whereas normal control rats received the same amount of olive oil on the same embryonic day. DNA damage was assessed by detecting γ-H2 A.X in spina bifida aperta rats. Real time-polymerase chain reaction was used to examine mRNA expression of MGMT in normal control and spina bifida aperta rats. In normal controls, the MGMT mRNA expression decreased with increasing embryonic days, and was remarkably reduced from E11 to E14, reaching a minimum at E18. In the spina bifida aperta model, γ-H2 A.X protein expression was increased, and mRNA expression of MGMT was markedly decreased on E14, E16, and E18. bisulfite sequencing polymerase chain reaction for MGMT promoter methylation demonstrated that almost all CpG sites in the MGMT promoter remained unmethylated in both spina bifida aperta rats and normal controls, and there was no significant difference in methylation level between the two groups on either E14 or E18. Our results show that DNA damage occurs in spina bifida aperta rats. The mRNA expression of MGMT is downregulated, and this downregulation is independent of promoter DNA methylation.

关键词： nerve regeneration neural tube defects spina bifida aperta spinal cord all-trans retinoic acid O6-methylguanine DNA methyltransferase gene expression DNA methylation promoter bisulfite sequencing polymerase chain reaction neural regeneration

A prototype of benzobis(imidazolium)-embedded conjugated polyelectrolyte: Synthesis by direct C–H arylation and fluorescent responses to anions

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Chinese Chemical Letters 2022年第5期33卷 2425-2428页

作者： Chuangui Yu Qinze Zheng Linhua Wang Tianbao Wang Xuesong Zheng Ge Gao Key Laboratory of Green Chemistry and Technology of Ministry of Education College of ChemistrySichuan UniversityChengdu 610064China

We report the convenient synthesis of a benzobis(imidazolium)-embedded conjugated polyelectrolyte pBBI by a Cu-catalyzed direct C–H arylation of a cationic benzobis(imidazolium)monomer with a di-iodide *** shows weak fluorescence in solution due to rotation of the repeat units in the conjugated backbone,and enhanced fluorescence when electrostatically interacting with a variety of an-ions to form ***,pBBI responds to the bisulfite anion with intensified unique deep-blue fluorescence easily discriminated by naked eye.

关键词： Conjugated polyelectrolyte(CPE) Benzobis(imidazolium) C–H arylation Fluorescent anion sensing bisulfite

Recent progress in Michael addition-based fluorescent probes for sulfur dioxide and its derivatives

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Chinese Chemical Letters 2018年第10期29卷 1456-1464页

作者： Junchao Xu Houqun Yuan Lintao Zeng Guangming Bao Institute of Veterinary Drug Jiangxi Agricultural University/Jiangxi Provincial Key Laboratory for Animal Health School of Chemistry and Chemical Engineering Tianjin University of Technology

Sulfur dioxide（SO_2） is a harmful environmental pollutant. Inhaled SO_2 can be rapidly hydrated into its derivatives, bisulfite（HSO_3^-） and sulfite（SO_3^（2-））. SO_2 derivatives are well known as preservatives and antioxidants, which are used in food and beverages to prevent oxidation and bacterial growth. Although SO_2 can be endogenously generated in mammals and exhibits unique bioactivities in regulating cardiovascular function, excessive SO_2 and its derivatives have toxic effects on humans and animals for triggering adverse reactions and diseases. A large number of fluorescent probes for SO_2 and its derivatives have been designed and reported due to their high sensitivity and selectivity, high temporal and spatial resolution, non-invasive and non-destructive detection as well as real-time visualization in situ. In this review, we have summarized the recent progress of Michael addition-based fluorescent probes for SO_2 and its derivatives. These probes are categorized and concluded according to the different α,β-unsaturated compounds（i.e., Michael acceptors）. The design strategies, sensing performances, detection mechanisms and applications of these probes are discussed in detailed. Finally, a general overview about the design of probes for SO_2 and its derivatives is provided, which will facilitate the development of ideal probes for SO_2 and its derivatives.

关键词： Fluorescent probes Michael addition Sulfur dioxide bisulfite Sulfite Application

Eagles report:Developing cancer biomarkers from genomewide DNA methylation analyses

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World Journal of Clinical Oncology 2011年第1期2卷 1-7页

作者： Wolfgang A Schulz Wolfgang Goering Department of Urology Medical FacultyHeinrich Heine University40225 DuesseldorfGermany

Analyses of DNA methylation in human cancers have identified hypermethylation of individual genes and diminished methylation at repeat elements as common alterations,and have thereby provided important mechanistic insights into cancer biology as well as biomarkers for cancer detection,prognosis and prediction of therapy *** techniques available in the past were best suited for investigations of individual candidate genes and sequences,whereas recently developed high-throughput techniques promise to generate unbiased and comprehensive surveys of DNA methylation states across entire *** this minireview we give a short overview of established and novel techniques and outline some major questions that can now be addressed to develop further cancer biomarkers and therapies based on DNA methylation.

关键词： bisulfite CpG-island Deep sequencing DNA hypermethylation Hypomethylation Methylcytosine-binding domain

Application of Silver Nanoparticles in Detection of DNA Methylation

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Application of Silver Nanoparticles in Detection of DNA Meth...

A stage-scanning laser confocal microscope and protocol for DNA methylation sequencing

第十一届全国化学生物学学术会议

作者： Ting Pi Xiao-Meng Tang Xiang-Juan Zheng Zhi-Mei Li Department of Chemistry School of ChemistryNanchang University

DNA methylation has been identified as one of the important causes of tumorigenesis,so it is important to develop some advanced methods for detecting and quantifying DNA *** this study,a label-free and enzyme-free colorimetric detection of DNA methylation based on unmodified Ag NPs has been *** strategy employs the difference in electrostatic attraction of single-stranded DNA and double-stranded DNA against salt-induced aggregation of Ag NPs after negative charged silver nanoparticles prepared with sodium borohydride as reducing agent are added to the hybrid system of DNA strand *** and quantitative analysis of gene methylation can be performed according to the color change and ultraviolet absorption spectrum change of Ag NPs *** experiment combines DNA bisulfite conversion technology to establish a marker-free,efficient and rapid colorimetric analysis method for gene methylation,which has broad application *** sensitive,simple and economical stratagem also provides a convenient and high-throughput platform for methylated p53 tumor suppressor gene detection in human serum samples,which displays a huge potential for clinical diagnosis and treatment of oncological diseases.

关键词： DNA methylation Ag NPs bisulfite Hybridization

来源： cnki会议评论

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Journal of Biomedical Science and Engineering 2010年第5期3卷 496-500页

作者： Vaithilingam Vaishnavi Litty Varghese Baquir Mohammed Jaffar Ali Life Sciences Division AU-KBC Research Centre Anna University

Recent understanding of the role of epigenetic regulation in health and disease has necessitated the development of newer and efficient methods to map the methylation pattern of target gene. In this article we report construction of a stage-scanning laser confocal microscope (SLCM) and associated protocol that determines the methylation status of target gene. We have adapted restricted Sanger’s sequencing where fluorescine labeled primers and dideoxy guanine fraction alone are used for target amplification and termination at cytosine positions. Amplified ssDNA bands are separated in 6% denaturing PAGE and scanned using SLCM to sequence the positions of methylated cytosines. We demonstrate that our me- thodology can detect < 100 femtomoles of DNA, and resolve the position of cytosine within ± 2 nucleotide. In a calibration run using a designer DNA of 99 bases, our methodology had resolved all the 11 cytosine positions of the DNA. We have further demonstrated the utility of apparatus by mapping methylation status in the Exon-1 region of a gene, E-Cadherin, in the plasma DNA sample of a healthy subject. We believe our approach constitute a low cost alternative to conventional DNA sequencers and can help develop methylation based DNA biomarkers for the diagnosis of disease and in therapeutics.

关键词： Scanning Confocal Microscope Fluorescence Detection Methylation bisulfite Sequencing DNA Sequencer