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Cocaine Detection in Blood Serum Using Aptamer Biosensor on Gold Nanoparticles and Progressive Dilution

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Chinese Journal of Chemistry 2012年第9期30卷 2023-2028页

作者：葛静刘肇芳赵新生 Beijing National Laboratory for Molecular Sciences State Key Laboratory for Structural Chemistry of Unstable and Stable SpeciesDepartment of Chemical BiologyCollege of Chemistry and Molecular Engineeringand Biodynamic Optical Imaging CenterPeking UniversityBeij'ing 100871China

To fight against the illegal usage of cocaine it is necessary to develop various analytical methodologies. We report here an aptamer-based biosensor for the determination of cocaine using gold nanoparticles as the fluorescence quencher. By employing the progressive dilution （PD） strategy, simultaneous qualitative and quantitative analysis of cocaine in blood serum was achieved without pretreatment of the sample. The method described in this paper of- fers significant improvement in the detection accuracy and can be used to quantify cocaine levels in complex bio- logical samples such as serum with a simple procedure.

关键词： aptamer biosensors cocaine serum progressive dilution

Overcome Debye Length Limitations for Biomolecule Sensing Based on Field Effective Transistors

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Chinese Journal of Chemistry 2021年第4期39卷 999-1008页

作者： Zhi Zheng Hongyuan Zhang Tianyou Zhai Fan Xia Engineering Research Center of Nano-Geomaterials of Ministry of Education Faculty of Materials Science and ChemistryChina University of GeosciencesWuhanHubei430074 China State Key Laboratory of Materials Processing and Die&Mould Technology School of Materials Science and EngineeringHuazhong University of Science and TechnologyWuhanHubei 430074Chino

biosensors based on field effective transistor(FET)have aroused tremendous attention in the past few years owning to their huge application in drug discovery,disease diagnosis and environmental *** FET biosensors possess small volume,high sensitivity at ultra-low concentration,considerable mechanical strength,as well as excellent stability in solution,which plays a vital role in the point of care testing(POCT)*** advances have summarized some progress involved in the improvement of morphology and structure of channel materials,the functionalization of organic molecule,the influence of device operation and sensing environment on the detecting ***,for FET biosensors,the charge screening phenomena were inevitable in the solution,which seriously degrade the device *** this article,we summarize recent advances to overcome debye length limitations for biomolecule sensing based on *** will firstly describe the charge screening mechanism,then focous on the strategy to overcome charge screening,including synthesizing special channel materials with crumpled morphology,designing aptamer binding mode,and modulating device ***,we discuss the major challenges and perspectives about overcoming debye length limitations of FET *** summaries provide further insights to realize real-time,lable-free,high-sensitivity FET sensors for medical healthcare.

关键词： Debye length Interactions Field effective transistors biosensors Biomolecule

Multiplexed Biosensing Diagnostic Platforms Detecting Autoantibodies to Tumor-Associated Antigens from Exosomes Released by CRC Cells and Tissue Samples Showed High Diagnostic Ability for Colorectal Cancer

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Engineering 2021年第10期7卷 1393-1412页

作者： Ana Montero-Calle Itziar Aranguren-Abeigon María Garranzo-Asensio Carmen Poves María Jesús Fernández-Aceñero Javier Martínez-Useros Rodrigo Sanz Jana Dziaková Javier Rodriguez-Cobos Guillermo Solís-Fernández Eloy Povedano Maria Gamella Rebeca Magnolia Torrente-Rodríguez Miren Alonso-Navarro Vivian de los Ríos J.Ignacio Casal Gemma Domínguez Ana Guzman-Aranguez Alberto Peláez-García JoséManuel Pingarrón Susana Campuzano Rodrigo Barderas Chronic Disease Program(UFIEC) Carlos III Health InstituteMadrid E-28220Spain Department of Biochemistry and Molecular Biology Faculty of Optics and OptometryComplutense University of MadridMadrid E-28040Spain Gastroenterology Unit San Carlos Clinical HospitalMadrid E-28040Spain Surgical Pathology Department San Carlos Clinical HospitalMadrid E-28040Spain Translational Oncology Division OncoHealth InstituteJimenez Diaz Foundation University HospitalMadrid E-28040Spain Department of Biochemistry Faculty of MedicineAlberto Sols Institute of Biomedical ResearchCSIC-UAMMadrid E-28029Spain Department of Analytical Chemistry Faculty of Chemical SciencesComplutense University of MadridMadrid E-28040Spain Center for Biological Research Spanish National Research Council(CSIC)MadridE-28040Spain Molecular Pathology and Therapeutic Targets Group La Paz University Hospital(IdiPAZ)Madrid E-28046Spain

Colorectal cancer(CRC)is the second leading cause of cancer-related death *** five-year survival rate of CRC patients depends on the stage at diagnosis,being higher than 80%when CRC is diagnosed in the early stages but lower than 10%when CRC is diagnosed in advanced *** against specific CRC autoantigens(tumor-associated antigens(TAAs))in the sera of patients have been widely demonstrated to aid in early ***,we herein aim to identify autoantigens target of autoantibodies specific to CRC that possess a significant ability to discriminate between CRC patients and healthy individuals by means of liquid *** that end,we examined the protein content of the exosomes released by five CRC cell lines and tissue samples from CRC patients by means of immunoprecipitation coupled with mass spectrometry analysis.A total of 103 proteins were identified as potential autoantigens specific to *** bioinformatics and meta-analysis,we selected 15 proteins that are more likely to be actual CRC autoantigens in order to evaluate their role in CRC prognosis by Western blot(WB)and immunohistochemistry(IHC).We found dysregulation at the protein level for 11 of these proteins in both tissue and plasma exosome samples from patients,along with an association of nine of these proteins with CRC *** validation,all but one showed a statistically significant high diagnostic ability to distinguish CRC patients and individuals with premalignant lesions from healthy individuals,either by luminescence Halotag-based beads,or by a multiplexed biosensing platform involving the use of magnetic microcarriers as solid support modified with covalently immobilized Halotag fusion proteins constructed for CRC *** together,our results highlight the usefulness of the approach defined here to identify the TAAs specific to chronic diseases;they also demonstrate that the measurement of autoantibody levels in plasma against the TAAs identified here

关键词： Autoantibodies Diagnosis Colorectal cancer Exosomes Tumor microenviroment Humoral immune response Point of care biosensors

Peptide aptamers:The versatile role of specific protein function inhibitors in plant biotechnology

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Journal of Integrative Plant Biology 2015年第11期57卷 892-901页

作者： Monica Colombo Chiara Mizzotti Simona Masiero Martin M.Kater Paolo Pesaresi Research and Innovation Centre Fondazione Edmund MachSan Michele all'Adige(Trento) Department of Biosciences University of Milan

In recent years, peptide aptamers have emerged as novel molecular tools that have attracted the attention of researchers in various fields of basic and applied science, ranging from medicine to analytical chemistry. These artificial short peptides are able to specifically bind, track, and inhibit a given target molecule with high affinity, even molecules with poor immunogenicity or high toxicity, and represent a remarkable alternative to antibodies in many different applications. Their use is on the rise, driven mainly by the medical and pharmaceutical sector. Here we discuss the enormous potential of peptide aptamers in both basic and applied aspects of plant biotechnology and food safety. The different peptide aptamer selection methods available both in vivo and in vitro are introduced, and the most important possible applications in plant biotechnology are illustrated. In particular, we discuss the generation of broad- based virus resistance in crops, ＂reverse genetics＂ and aptasensors in bioassays for detecting contaminations in food and feed. Furthermore, we suggest an alternative to the transfer of peptide aptamers into plant cells via genetic transformation, based on the use of cell-penetrating peptides that overcome the limits imposed by both crop transformation and Genetically Modified Organism commercialization.

关键词： biosensors functional genomics peptide aptamers resistance

Developing a Nano-Biosensor for DNA Hybridization Using a New Electroactive Label

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Chinese Journal of Chemistry 2011年第11期29卷 2541-2551页

作者： Raoof, Jahan Bakhsh Ojani, Reza Ebrahimi, Maryam Hamidi-Asl, Ezat Electroanalytical Chemistry Research Laboratory Department of Analytical Chemistry Faculty of Chemistry University of Mazandran Babolsar Iran

Development of electrochemical DNA hybridization biosensors based on carbon paste electrode （CPE） and gold nanoparticle modified carbon paste electrode （NGMCPE） as transducers and ethyl green （EG） as a new electroac- tive label is described. Electrochemical impedance spectroscopy and cyclic voltammetry techniques were applied for the investigation and comparison of bare CPE and NGMCPE surfaces. Our voltammetric and spectroscopic studies showed gold nanoparticles are enable to facilitate electron transfer between the accumulated label on DNA probe modified electrode and electrode surface and enhance the electrical signals and lead to an improved detection limit. The immobilization of a 15-mer single strand oligonucleotide probe on the working electrodes and hybridiza- tion event between the probe and its complementary sequence as a target were investigated by differential pulse voltammetry （DPV） responses of the EG accumulated on the electrodes. The effects of some experimental variables on the performance of the biosensors were investigated and optimum conditions were suggested. The selectivity of the biosensors was studied using some non-complementary oligonucleotides. Finally the detection limits were calculated as 1.35×10^-10 mol/L and 5.16×10^-11 mol/L on the CPE and NEGCPE, respectively. In addition, the bio-sensors exhibited a good selectivity, reproducibility and stability for the determination of DNA sequences.

关键词： ethyl green hybridization detection DNA recognition gold nanoparticle modified carbon paste elec- trode biosensors nucleotides

Insight into the effects of electrochemical factors on host-guest interaction induced signature events in a biological nanopore

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纳米技术与精密工程（英文） 2020年第1期3卷 2-8页

作者： XiaojunWei Zehui Zhang XiaoqinWang Brian Lenhart Roberto Gambarini Jonathan Gray Chang Liu Biomedical Engineering Program University of South CarolinaColumbiaSC 29208USA Department of Chemical Engineering University of South CarolinaColumbiaSC 29208USA

The signature events caused by host-guest interactions in the nanopore system can be used as a novel and characteristic signal in quantitative detection and analysis of various ***,the effect of several electrochemical factors on the host-guest interactions in nanopore still remains ***,we systematically studied host-guest interactions,especially oscillation of DNA-azide adamantane@cucurbit[6]inα-Hemolysin nanopore under varying pH,concentration of electrolytes and counterions(Li+,Na+,K+).Our results indicate correlations between the change of pH and the duration of the oscillation *** addition,the asymmetric electrolyte concentration and the charge of the counterions affects the frequency of signature events in oscillation signals,and even the integrity of the protein *** study provides insight into the design of a future biosensing platform based on signature oscillation signals of the host-guest interaction within a nanopore.

关键词： Nanopore Host-guest interaction biosensors Electrochemistry

New Dopa-AIE Compound Used as Fluorescence Sensor Material： Specificity and Quantification for Cu（Ⅱ）

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Chinese Journal of Chemistry 2017年第3期35卷 335-340页

作者： Siwei Liu Jing Cheng Jiarui Xu PCFMLab and GDHPPC Lab School of Chemistry Sun Yat-sen University Guangzhou Guangdong 510275 China Department of Materials Soience and Engineering and Bioengineering Univer~s'ity of California Berkeley CA 94702 U. S. A.

As a paramagnetic metal ion, Cu(II) is challenging to be detected by conventional fluorescence sensor because of its unselective paramagnetic quenching effects on common fluorophores. By combining catechol with tetraphenylethene, a typical aggregation‐induced emission (AIE) moiety, we have successfully synthesized a new kind of catechol, the dopa‐AIE compound. Due to the properties of AIE core, the catechol has the “turn on” mechanism as a fluorescence chemosensor. Specifically, the new catechol has the unique detection selectivity on Cu(II) in solution. The fluorescence intensity has the maximum value when the molar ratio of the catechol to Cu(II) is 1∶1. Before and after this point, the fluorescence intensity shows a linear relationship with the copper ion concentration, increasing or decreasing. Thus, the quantitative calculation for Cu(II) or the dopa‐AIE compound could be done according to this response process.

关键词： dopamines biosensors copper fluorescence redox

Recent advances in electrogenerated chemiluminescence biosensing methods for pharmaceuticals

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Journal of Pharmaceutical Analysis 2019年第1期9卷 9-19页

作者： Yu Zhang Rui Zhang Xiaolin Yang Honglan Qi Chengxiao Zhang Medpace Bioanalytical Laboratories School of Informatics Computing and Engineering Indiana University Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province School of Chemistry and Chemical Engineering Shaanxi Normal University

Electrogenerated chemiluminescence(electrochemiluminescence, ECL) generates species at electrode surfaces, which undergoes electron-transfer reactions and forms excited states to emit light. It has become a very powerful analytical technique and has been widely used in such as clinical testing, biowarfare agent detection, and pharmaceutical analysis. This review focuses on the current trends of molecular recognition-based biosensing methods for pharmaceutical analysis since 2010. It introduces a background of ECL and presents the recent ECL developments in ECL immunoassay(ECLIA), immunosensors, enzyme-based biosensors, aptamer-based biosensors, and molecularly imprinted polymers(MIP)-based sensors. At last, the future perspective for these analytical methods is briefly discussed.

关键词： Electrogenerated chemiluminescence Pharmaceutical analysis Immunoassay biosensors

A Review on Graphene-Based Nanomaterials in Biomedical Applications and Risks in Environment and Health

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Nano-Micro Letters 2018年第3期10卷 164-197页

作者： Thabitha P.Dasari Shareena Danielle McShan Asok K.Dasmahapatra Paul B.Tchounwou RCMI Center for Environmental Health Jackson State University

Graphene-based nanomaterials(GBNs) have attracted increasing interests of the scientific community due to their unique physicochemical properties and their applications in biotechnology, biomedicine, bioengineering, disease diagnosis and therapy. Although a large amount of researches have been conducted on these novel nanomaterials, limited comprehensive reviews are published on their biomedical applications and potential environmental and human health effects. The present research aimed at addressing this knowledge gap by examining and discussing:(1) the history, synthesis,structural properties and recent developments of GBNs for biomedical applications;(2) GBNs uses as therapeutics,drug/gene delivery and antibacterial materials;(3) GBNs applications in tissue engineering and in research as biosensors and bioimaging materials; and(4) GBNs potential environmental effects and human health risks. It also discussed the perspectives and challenges associated with the biomedical applications of GBNs.

关键词： Graphene-based nanomaterials Biomedical Delivery biosensors Tissue engineering Bioimaging Health and environment risks