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Application of microwaves in Chemsitry and bioprocessing

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Application of microwaves in Chemsitry and Bioprocessing

Sustainable arabitol production by a newly isolated Debaryomyces prosopidis strain cultivated on biodiesel-derived glycerol

第十二届全国微波能应用学术会议

作者： G．S．Vijaya Raghavan Department of Bioresource Engineering McGill University 21111 Lakeshore RoadSte-Anne-de-BellevueQCH9X 3V9Canada

The special microwave-matter interaction mechanisms make microwaves a special format of energy that can bring many new characteristics into its various applications such as synthesis, extraction, drying, and culturing processes. The common characteristics in drying processes are the great reduction in processing time and better end-product quality. In the extraction process, processing time reduction is still an attractive point. Higher yield, and better quality extracts are also obtained using microwave-assisted extraction method. Microwaves also have some special biological effect such as increasing viability of seeds and stimulating the root formation from cotyledon, which can be used in culturing processes.

关键词： drying MAE culturaing bioprocessing microwaves synthesis extraction natural product

来源： cnki会议评论

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Carbon Resources Conversion 2022年第1期5卷 92-99页

作者： Rosanina Filippousi Erminta Tsouko Kerryane Mordini Dimitrios Ladakis Apostolis AKoutinas George Aggelis Seraphim Papanikolaou Department of Food Science and Human Nutrition Agricultural University of Athens75 Iera Odos11855 AthensGreece Unit of Microbiology Department of BiologyUniversity of Patras26504–Rio PatrasGreece

The purpose of this research was the bioconversion of biodiesel-derived glycerol to arabitol through the adoption of sustainable *** production by Debaryomyces prosopidis FMCC Y69 was significantly affected by initial glycerol *** highest arabitol concentration of 45.5 g/L was achieved in the case of 120 g/L initial glycerol combined with the highest yield(0.379 g arabitol/g initial glycerol).The yeast strain showed a non-typical oleaginous behavior with low accumulation of lipids(up to 20.9%w/w)despite nitrogen-limited condition that prevailed in the fermentation ***(IPs)were considerably high with a yield on the produced dry cell weight(YIPs/X)(=40.9%w/w)when 85 g/L of glycerol was utilized as the starting *** fermentation efficiency was further improved when the optimal carbon to nitrogen(C/N)ratio was *** specifically,arabitol production was maximized(reaching 56.5 g/L)at a C/N ratio of 158 moles/moles while yield and productivity were also enhanced with respective values of 0.48 g arabitol/g consumed glycerol and 0.112 g/*** increase in C/N ratios led to a decrease in cell dry weight(DCW)concentrations,which is rationally associated with decreased nitrogen availability in the growth ***/X values were remarkably high(23.1–26.1%w/w)at the end of each fermentation with the highest concentration in absolute values of 7.3 g/L achieved at the lowest C/N ratio employed(i.e.,79 moles/moles).This study demonstrated the efficient arabitol production along with other value-added metabolites,such as IPs and cellular lipids,implementing biotechnology and renewable resources and thus contributing to the development of circular economy technologies.

关键词： bioprocessing Renewable resources Newly isolated yeast Arabitol production Value added metabolites

Bioprocessed Black Rice Bran Potentiates the Growth Inhibitory Activity of an Immune Checkpoint Inhibitor against Murine Colon Carcinoma

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Food and Nutrition Sciences 2023年第12期14卷 1149-1171页

作者： Kyung Hee Lee Ki Sun Kwon Woon Sang Hwang Wha Young Lee Jeanman Kim Sang Jong Lee Sung Phil Kim Mendel Friedman STR Biotech Co. Ltd. Chuncheon Republic of Korea U.S. Department of Agriculture Western Regional Research Center Agricultural Research Service Albany CA USA

This study determined the effect of orally fed polysaccharide-rich bioprocessed (fermented) black rice bran produced by culturing with shiitake (Lentinus edodes) mushroom mycelium on CT-26 colon cancer cells in vivo in an intracutaneously transplanted mouse tumor alone and in combination with intraperitoneally administered anti-PD-1 immune checkpoint inhibitor. Analysis of the isolated tumor weights at the end of the study shows that the average tumor size in control mice is 3.78 grams, and the average tumor size in mice treated with anti-PD-1 antibody is 2.16 grams. The average tumor size in mice treated with BRB-F alone is 2.25 grams, and the average tumor size in mice treated with anti-PD-1 antibody BRB-F combination is 1.38 grams. Thus, BRB-F or anti-PD-1 antibody alone each reduce tumor size by 40.5% or 42.9%, whereas the combination of BRB-F and anti-PD-1 antibody reduces tumor size by 63.5%, with their cooperative effect being statistically significant. The observed anti-tumor effects were accompanied by a series of biomarkers associated with cancer formation and inhibition. These results indicate that the reported potentiation of cancer therapy using drug-based medical chemotherapies with added checkpoint inhibitors in human patients are mechanistically similar with the functional food evaluated in the present study. These beneficial effects in mice challenge clinicians to investigate if the black rice bran food product can also protect against human cancer.

关键词： Black Rice Bran Mushroom Mycelia bioprocessing Immune Checkpoint Inhibitor Mice Tumor Regression Cancer Prevention Biomarkers Mechanism Research Needs

Switchgrass (Panicum virgatum) Fermentation by Clostridium thermocellumand Clostridium beijerinckiiSequential Culture: Effect of Feedstock Particle Size on Gas Production

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Advances in Microbiology 2015年第5期5卷 311-316页

作者： Michael D. Flythe Noelia M. Elía Micah B. Schmal Sue E. Nokes USDA Agricultural Research Service Forage-Animal Production Research Unit Lexington USA Department of Animal and Food Sciences University of Kentucky Lexington USA Department of Biosystems and Agricultural Engineering University of Kentucky Lexington USA

Fermentation of cellulosic biomass can be done in a single step with cellulolytic, solventogenic bacteria, such as Clostridium thermocellum. However, the suite of products is limited in consolidated bioprocessing. Fortunately, the thermophilic nature of C. thermocellum can be exploited in sequential culture. Experiments were conducted to determine the effect of feedstock particle size on fermentation by sequential cultures and to demonstrate this effect could be shown by gas production. Dual-temperature sequential cultures were conducted by first culturing with C. thermocellum (63°C, 48 h) before culturing with C. beijerinckii (35°C, 24 h). Switchgrass (2, 5 or 15 mm particle size) was the feedstock in submerged substrate (10% w/v) fermentation. The extent of fermentation was evaluated by gas production and compared by analysis of variance with Tukey’s test post hoc. C. thermocellum alone produced 78 kPa cumulative pressure (approx. 680 mL gas) when the particle size was 2 or 5 mm. The C. thermocellum cultures with 15 mm feedstock particles had a mean cumulative pressure of 15 kPa after 48 h, which was less than the 2 and 5 mm treatments (P °C) and inoculated with C. beijerinckii, and the cumulative pressures were reset to ambient, cumulative pressure values as great as 70 kPa (equivalent to an additional 670 mL gas) were produced in 24 h. Again, the longer (15 mm) particle size produced less gas (P < 0.05). When the substrates were inoculated with C. beijerinckii without previous fermentation by C. thermocellum, the mean cumulative pressures were approximately 10 kPa. These results indicate that biological pretreatment with C. thermocellum increased the availability of switchgrass carbohydrates to C. beijerinckii, and that gas production is suitable method to show the effectiveness of a pretreatment.

关键词： Bioenergy Cellulosic Butanol Co-Culture Consolidated bioprocessing

Development of Medium and Kinetic Modeling for Enhanced Production of Cutinase from Pseudomonas cepacia NRRL B-2320

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Advances in Microbiology 2013年第6期3卷 479-489页

作者： Kasturi Dutta Veeranki Venkata Dasu Krishnamoorthy Hegde Biochemical Engineering Laboratory Department of Biotechnology Indian Institute of Technology Guwahati Guwahati India

The development of medium for the production of cutinase from Pseudomonas cepacia NRRL B 2320 was carried out using Plackett-Burman experimental design followed by central composite design. The medium components were screened by Plackett-Burman experimental design which suggested that cutin, peptone, KCl and MgSO4·7H2O have influenced the cutinase production significantly with very high confidence levels. The concentration levels of these four components were optimized using 24 full factorial central composite design. An optimum combination of 10.06 g·L-1 of cutin, 17.77 g·L-1 of peptone, 0.635 g·L-1 of KCl and 5.455 g·L-1 of MgSO4·7H2O in the medium gave a maximum cutinase activity of 336 U·mL-1. An overall 2 fold increase in the production of cutinase was observed in the optimized medium. Growth and production of cutinase from P. cepacia NRRL B 2320 have been studied in shake flask and batch bioreactor. Time course of cell growth and enzyme production was fitted to the existing kinetic models reported in the literature to estimate the biokinetic parameters. These models suggested that the production of cutinase is growth associated in shake flask and it is a mixed growth type in a batch bioreactor.

关键词： Cutinase Kinetics bioprocessing Optimization Parameter Identification