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Gonioscopy and Ultrasound biomicroscopy in the Detection of Angle Closure in Patients with Shallow Anterior Chamber

Chinese Medical Sciences Journal 2014年第4期29卷 204-207页

作者： Shan-shan Cui Yan-hong Zou Qian Li Li-na Li Ning Zhang Xi-pu Liu Department of Ophthalmology First Hospital of Tsinghua University Medical Center Tsinghua University Department of Ophthalmology Sekwa Eye Hospital

Objective To assess the agreement between gonioscopy and ultrasound biomicroscopy(UBM) in detecting angle closure in Chinese patients with shallow anterior chamber. Methods An observational comparative study of the two different examination methods was conducted. Patients with normal intraocular pressure and temporal peripheral anterior chamber depth less than a quarter of corneal thickness based on slit lamp examination were included in this study from December 2007 to May 2009 in the outpatient clinic of First Hospital of Tsinghua University. Gonioscopy was performed with a Goldman goniolens in dark room first and followed by full beam light and indentation. If the filtering trabecular meshwork was invisible or any peripheral anterior synechia was found, that quadrant of the angle was considered closed. UBM was first undertaken in a darkened room then repeated with normal room lighting. If iridotrabecular apposition was showed, that quadrant of the angle was considered closed. The status of angle closure of each quadrant with different methods was recorded. Results 85 eyes of 46 patients were included in this study. The agreement between gonioscopy and UBM was poor(κ<0.4) with Kappa analysis in both dark and light conditions in each quadrant. The accordance of agreement between gonioscopy and UBM was hardly affected by age or sex, while in dark condition, eyes with deeper anterior chamber(P=0.005) or plateau iris configuration tended to produce different results(P=0.075) in the 2 methods. Conclusion Gonioscopy and UBM are both indispensable methods for detecting angle closure, neither can completely replace the other.

关键词： gonioscopy ultrasound biomicroscopy angle closure glaucoma

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Simultaneous follow-up of mouse colon lesions by colonoscopy and endoluminal ultrasound biomicroscopy

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World Journal of Gastroenterology 2013年第44期19卷 8056-8064页

作者： Rossana C Soletti Kelly Z Alves Marcelo AP de Britto Dyanna G de Matos Mnica Soldan Helena L Borges Joo C Machado Biomedical Engineering Program COPPE Federal University of Rio de Janeiro Rio de Janeiro RJ 21941-972 Brazil Post-Graduation Program in Surgical Sciences Department of Surgery School of Medicine Federal University of Rio de Janeiro Rio de Janeiro RJ 21941-972 Brazil Biomedical Science Institute Federal University of Rio de Janeiro Rio de JaneiroRJ 21941-972 Brazil Division of Gastroenterology Endoscopy UnitClementino Fraga Filho University Hospital Federal University of Rio de Janeiro RJ 21941-972 Brazil

AIM:To evaluate the potential use of colonoscopy and endoluminal ultrasonic biomicroscopy(eUBM)to track the progression of mouse colonic ***:Ten mice were treated with a single azoxy-methane intraperitoneal injection(week 1)followed by seven days of a dextran sulfate sodium treatment in their drinking water(week 2)to induce inflammationassociated colon *** was performed simultaneously with colonoscopy at weeks 13,17-20 and21.A 3.6-F diameter 40 MHz mini-probe catheter was used for eUBM *** ultrasound mini-probe catheter was inserted into the accessory channel of a pediatric flexible bronchofiberscope,allowing simultaneous acquisition of colonoscopic and eUBM *** image acquisition,the mice were anesthetized with isoflurane and kept in a supine position over a stainless steel heated surgical waterbed at 37℃.Both eUBM and colonoscopic images were captured and stored when a lesion was detected by colonoscopy or when the eUBM image revealed a modified colon wall *** the procedure,the colon was irrigated with water that was injected through a flush port on the mini-probe catheter and that acted as the ultrasound coupling medium between the transducer and the colon *** the acquisition of the last eUBM/colonoscopy section for each animal was completed,the colons were fixed,paraffin-embedded,and stained with hematoxylin and *** images acquired at the first time-point for each mouse were compared with subsequent eUBM/colonoscopic images of the same sites obtained in the following acquisitions to evaluate lesion ***:All 10 mice had eUBM and colonoscopic images acquired at week 13(the first time-point).Two animals died immediately after the first imaging acquisition and,consequently,only 8 mice were subjected to the second eUBM/colonoscopy imaging acquisition(at the second time-point).Due to the advanced stage of colonic tumorigenesis,5 animals died after the second time-point image acquisition,and t

关键词： Ultrasound biomicroscopy Animal model Diagnostic imaging Colonic neoplasm Longitudinal study

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A doublet mechanics model for the ultrasound characterization of malignant tissues

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Journal of Biomedical Science and Engineering 2011年第5期4卷 362-374页

作者： Francesco Gentile Jason Sakamoto Raffaella Righetti Paolo Decuzzi Mauro Ferrari 不详

Non invasive ultrasound-based imaging systems are being more commonly used in clinical bio-microscopy applications for both ex vivo and in vivo analysis of tissue pathological and physiological states. These modalities usually employ high-frequency ultrasound systems to overcome spatial resolution limits of conventional clinical diagnostic approaches. Biological tissues are non continuous, non homogeneous and exhibit a multiscale organization from the sub-cellular level (￡1 mm) to the organ level (31 cm). When the ultrasonic wavelength used to probe the tissues becomes comparable with the tissue's microstructure scale, the propagation and reflection of ultrasound waves cannot be fully interpreted employing classical models developed within the continuum assumption. In this study, we present a multiscale model for analyzing the mechanical response of a non-continuum double-layer system exposed to an ultrasound source. The model is developed within the framework of the Doublet Mechanics theory and can be applied to the non-invasive analysis of complex biological tissues.

关键词： Nanomechanics Doublet Mechanics Ultrasound Spectroscopy Biopsy Microscopic Elastography Ultrasound biomicroscopy

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Primary Pupillary Margin Cyst of the Iris Pigment Epithelium

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Chinese Medicine 2011年第1期2卷 16-19页

作者： R. Dammacco G. Giancipoli S. Guerriero D. Piscitelli N. Cardascia 不详

Purpose: Description of a patient with a solitary cyst of the pupillary margin iris pigment epithelium (IPE). Methods: A 63-year-old man referred a suspected iris-ciliary body melanoma in his left eye. Based on both clinical examination and ultrasound biomicroscopy, melanoma was considered unlikely. Surgery was under-taken to correct recurrent deterioration of vision due to movement of the lesion across the visual axis. Results: The lesion was excised completely. Ultrasound biomicroscopy and histopathological examination ruled out melanoma and allowed a final diagnosis of primary pupillary margin cyst of the IPE, characterized of pig-mented epithelium, with no connective tissue or vessels. No recurrences or fresh lesions appeared during a one-year follow-up. Conclusions: Primary epithelial iris cysts are usually benign. Treatment is required only in symptomatic patients and those with an uncertain diagnosis. Ultrasound biomicroscopy is indispensable to confirm the clinical diagnosis, follow the clinical course and intervene if surgery is required.

关键词： Melanoma Iris Pigment Epithelium Pupillary Cyst Surgical Excision Ultrasound biomicroscopy

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