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The biomass dosage Influences the Effects of Diethyl Aminoethyl Hexanoate on Micropropagation of Echinacea purpurea (L.) Moench

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Journal of Biosciences and Medicines 2016年第12期4卷 97-110页

作者： Xiaolu Chen Dongliang Li Junjie Zhang Qingling Li Yuesheng Yang Hong Wu Tropical Crops Genetic Resources Institute Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China Ministry of Agriculture Danzhou China College of Life Science South China Agricultural University Guangzhou China Institute of Tropical Horticulture Research Hainan Academy of Agricultural Science Haikou China

The plant growth regulator diethyl aminoethyl hexanoate (DA-6) has proved highly effective on micropropagation of the medicinal plant purple coneflower (Echinacea purpurea (L.) Moench), however, sharp variation of the effects existed among explants in the same treatment, making the application of DA-6 in micropropagation difficult. In order to clarify factors that influencing the treating results of DA-6, explants with different biomass dosage were prepared and inoculated onto medium supplemented with different concentrations of DA-6. It was found that among the three kinds of biomass dosage explants, the lowest biomass explants required the lowest concentration of DA-6, and the highest biomass explants required the highest concentration of DA-6 for the best results on adventitious buds regeneration. Similar results were obtained when regenerated buds of three different biomass dosages were cultured. It could be concluded from the above experimental results that for achieving better DA-6 application results, the concentration of DA-6 should be determined not only by the types but also by the biomass dosage of the explants. The present finding might help to improve the micropropagation efficiency in E. purpurea, and might be applicable for other species

关键词： Plant Growth Regulator DA-6 Micropropagation biomass dosage Echinacea purpurea

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Biosorption of Cd (Ⅱ) and Pb (Ⅱ) Ions by Aqueous Solutions of Novel Alkalophillic Streptomyces VITSVK5 spp. biomass

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Journal of Ocean University of China 2011年第1期10卷 61-66页

作者： Kumar Saurav Krishnan Kannabiran Biomolecules and Genetics Division School of Biosciences and TechnologyVIT University

Discharge of heavy metals from metal processing industries is known to have adverse effects on the ***-sorption of heavy metals by metabolically inactive biomass of microbial organisms is an innovative and alternative technology for removal of these pollutants from aqueous *** search of marine actinobacteria with potential heavy metal biosorption ability resulted in the identification of a novel alkalophilic Streptomyces VITSVK5 *** biosorption property of Streptomyces VITSVK5 *** investigated by absorbing heavy metals Cadmium (Cd) and Lead (Pb).Physiochemical characteristics and trace metal concentration analysis of the backwater showed the concentrations of different metals were lead 13±2.1 μg L-1,cadmium 3.1±0.3μg L-1,zinc 8.4±2.6μg L-1 and copper 0.3±0.1μg L-1,whereas mercury was well below the detection *** effect of pH and biomass dosage on removal efficiency of heavy metal ions was also *** optimum pH for maximal biosorption was 4.0 for Cd (II) and 5.0 for Pb (II) with 41% and 84% biosorption *** biosorbent dosage was optimized as 3 g L-1 for both the trace *** transform infrared absorption spectrum results indicated the chemical interactions of hydrogen atoms in carboxyl (–COOH),hydroxyl (–CHOH) and amine (–NH2) groups of biomass with the metal *** could be mainly involved in the biosorption of Cd (II) and Pb (II) onto Streptomyces VITSVK5 *** results of our study revealed Streptomyces metabolites could be used to develop a biosorbent for adsorbing metal ions from aqueous environments.

关键词： heavy metals biosorption Streptomyces VITSVK5 spp pH biomass dosage

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