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Automated detection of parasitized Cadra cautella eggs by Trichogramma bourarachae using machine vision

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International Journal of Agricultural and biological Engineering 2018年第3期11卷 94-101页

作者： Mohammed S.El-Faki Yuqi Song Naiqian Zhang Hamadttu A.El-Shafie Pan Xin Date Palm Research Center of Excellence King Faisal UniversityAl-AhsaKingdom of Saudi Arabia Department of biological and Agricultural Engineering College of EngineeringKansas State UniversityManhattanKS 66506USA College of Computer and Information Engineering Inner Mongolia Agricultural UniversityHohhot 010018Inner MongoliaChina

Cadra(Ephestia)cautella(Walker)is a moth that attacks dates from ripening stages while on tree,throughout storage,and until consumption,causing enormous qualitative and quantitative damages,resulting in economic ***-processing algorithms were developed for detecting and differentiating between three Cadra egg categories based on the success of Trichogramma bourarachae(Pintureau and Babaul)*** categories were parasitized(black and dark red),unparasitized fertile unhatched(yellow),and unparasitized hatched(white)***,light intensity,and shape information was used to develop detection *** image processing methods were developed based on three randomly selected images and were tested on a larger validation image set of 40 images:(i)segmentation and extractions of color and morphological features followed by Watershed delineation,and is referred to as Algorithm 1(ALGO1),(ii)finding circular objects by Hough Transformation followed by convolution filtering,and is referred to as Algorithm 2(ALGO2).ALGO1 and ALGO2 achieved correct classification rates(CCRs)for parasitized eggs of 92%and 96%,*** CCRs for unhatched eggs were 48%and 94%,and for hatched eggs were 42%and 73%,*** parasitized eggs,both methods performed satisfactorily,but,in general,ALGO2 outperformed *** results ensure automatic evaluation of the efficiency of biological control of Cadra cautella by the egg parasitoid Trichogramma bourarachae by quantifying the rate of *** developed detection methods can be used by producers of biocontrol agents for online monitoring of Trichogramma and similar insect natural enemies during mass production and before release against crop ***,with few adjustments these methods can be used in similar applications such as detecting plant diseases.

关键词： Trichogramma parasitization Cadra eggs detection machine vision date fruit image processing objects recognition algorithms biological control

Soil fungi of three native tree species inhibit biomass production and shift biomass allocation of invasive Mikania micrantha Kunth

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Science Bulletin 2013年第7期58卷 757-765页

作者： GAO Lei ZAN QiJie LI MingGuang GUO Qiang HU Liang JIANG Lu ZHOU Sheng LIU HaiJun State Key Laboratory of Biocontrol Sun Yat-sen University Guangdong Key Laboratory of Plant Resources Shenzhen Wild Animal Rescue Center School of Life Sciences Shenzhen University Shenzhen Wildlife Protecting Administration 6. School of Geographical Science and Planning Sun Yat-sen University

Soil microbes contribute to native plant species successful resistance against invasive *** native tree species,Heteropanax fragrans (HF),Cinnamomum burmanii (CB),and Macaranga tanarius (MT) were effective in controlling the notorious invasive vine Mikania micrantha (MM).Biomass production and allocation patterns (shoot/root biomass ratio (shoot/root)) are important indicators of MM climbing coverage and competitive light-capturing *** investigation was conducted to test the role of soil microbes associated with the three native tree species to inhibit MM biomass production and shift MM shoot/*** soils originating from preculture HF,CB,MT,and MM plots were collected separately for use as *** inocula were mixed with sterilized river sand at a 1:9 (w/w) ratio to grow *** fungicide carbendazim (methyl benzimidazol-2-ylcarbamate) was applied to half the treatments to kill pathogenic soil *** nutrient levels were established based on the natural soil nutrient concentration from a field stand invaded by *** were grown from seeds in a glasshouse,harvested 15 weeks after sowing,and separated into shoot and root *** showed that under interaction of soil origin and nutrient levels,MM biomass production was unchanged,but biomass allocation patterns were significantly *** biomass production grown in the three native tree soils under two nutrient levels was similar or higher than MM biomass production in MM conspecific soil,indicating the absence of species-specific pathogens that inhibited MM biomass production in native tree ***,in both conspecific and tree soils,MM biomass production was significantly reduced in the presence of pathogenic soil fungi,*** experienced significant fungal inhibition,demonstrating the pathogenic soil fungi promoted native tree resistence to *** exhibited decreased shoot biomass allocation when cultivated in native tree soil relative to MM conspecific soil under fie

关键词： biological control biotic resistance Cinnamomum burmanii Heteropanax fragrans invasion ecology Macaranga tanarius Mikania micrantha nutrient levels pathogenic fungi

Screening of Bacillus subtilis HAAS01 and Its Biocontrol Effect on Fusarium wilt in Sweet Potato

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Phyton-International Journal of Experimental Botany 2022年第8期91卷 1779-1793页

作者： Chengyang Li Lianjun Wang Shasha Chai Yinghua Xu Chong Wang Yi Liu Jian Lei Xiaojie Jin Xianliang Cheng Yuanyuan Yang Xinsun Yang Wenying Zhang Food Crops Institute of Hubei Academy of Agricultural Sciences Hubei Engineering and Technology Research Center of Sweet PotatoHubei Key Laboratory of Food Crop Germplasm and Genetic ImprovementWuhan430064China Research Center of Crop Stresses Resistance Technologies Yangtze UniversityJingzhou434025China

Fusarium wilt,a disease caused by Fusarium oxysporum *** batatas(Fob)is an important disease in sweet potato *** endophytic bacteria for biological control of sweet potato diseases is one of the important ways.A Bacillus subtilis with antagonistic effect on Fusarium wilt of sweet potato was isolated from soil by confrontation *** to the biological characteristics,16S rDNA sequence analysis,and physiological and biochemical analysis,the Bacillus subtilis HAAS01 was named.A pot experiment was conducted for the biological control experiment of strain HAAS01,and the endogenous hormone content,antioxidant enzyme activity,soluble protein content,and related gene expressions of sweet potato plants were *** results showed that the HAAS01 strain could promote the production of endogenous hormones and resist the infection of plant diseases together with defensive enzymes and upregulation of related gene *** summary,Bacillus subtilis HAAS01 was effective in controlling Fusarium wilt of sweet potato and has potential for application and development.

关键词： Bacillus subtilis HAAS01 Ipomoea batatas(L.)Lam. Fusarium wilt Fusarium oxysporum f.sp batatas biological control

control Alternatives for Damping-Off in Tomato Seedling Production

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Phyton-International Journal of Experimental Botany 2019年第3期88卷 325-333页

作者： A.C.Michel-Aceves J.F.Díaz-Nájera R.Ariza-Flores M.A.Otero-Sánchez R.Escobar-Martínez C.H.Avendaño-Arrazate Centro de Estudios Profesionales del Colegio Superior Agropecuario del Estado de Guerrero Av.Vicente Guerrero No.81Colonia CentroIgualaGuerreroC.P.40000México Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias(INIFAP)Campo Experimental IgualaCarr.Iguala-TuxpanKm.2.5 IgualaGuerreroMéxico INIFAP-Campo Experimental Rosario Izapa.Carr.Tapachula-Cacahoatán Tuxtla chicoChiapasC.P.30870México

In two tomato genotypes,we assessed control alternatives for damping-off with combinations of chemical fungicides and native/commercial strains of biological *** treatments consisting of 19 levels of mixing products,chemical fungicides,native strains and commercial products from biological control agents,and untreated treatment were used onto Ramsés and Toro *** were distributed on an incomplete block design in divided plots arrangement,where genotypes constitute the larger ones and the 8-repetition mixed products,the smaller *** 180 mL of fungal complexes,made of spores and mycellium Fusarium-solani(2×106 UFC),Rhizoctonia-solani(1×106 UFC),Phytophthora-capsici(1×105 UFC)and Sclerotium-rolfsii(mycellium-sclerotia)on each seedling trays,made inoculation *** mixtures of(Bacillus spp.+Streptomyces spp.+Trichoderma spp.)+(Propamocarb+Fosetyl);(Bacillus spp.+Streptomyces spp.+Trichoderma spp.)+(Metalaxyl+Chlorothalonil);Pseudomonas fluorescens+Streptomyces+Micromonospore+Sporideamium+Aminoacidos,Péptidos,Carbohidratos)+(Propamocarb+Fosetyl);the native strain of Trichoderma asperellum+(Propamocarb+fosetil)and the native strains Trichoderma asperellum+Bacillus subtilis,diminished damping-off,prevented its appearance and had most significant agronomic *** contrast to this,combination of(Mancozeb)+(Free iodine)+(Metalaxyl+Chlorothalonil)+(Methyl thiophanate)produced some toxicity in the *** addition,Ramsés presented the best agronomic parameters,while Toro had the utmost fresh and dry weight in root.

关键词： Trichoderma harzianum T.lignorum biological control

In vitro evaluation of native entomopathogenic fungi and neem(Azadiractha indica)extracts on Spodoptera frugiperda

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Phyton-International Journal of Experimental Botany 2019年第1期88卷 47-54页

作者： Hernandez-Trejo A B Estrada-Drouaillet JA Lopez-Santillan C Rios-Velasco SE Varela-Fuentes R Rodriguez-Herrera E Osorio-Hernandez Postgraduate Studies and Research Division Autonomous University of TamaulipasSchool of Engineering and Sciences.Centro Universitario Adolfo Lopez MateosCd.VictoriaTamaulipasC.P.87000Mexico Center for Research in Food and Development A.C.Cuauhtemoc Unit.Laboratory of Postharvest PhysiologyPlant Pathology and Biological Control.Av.Rio Conchos s/n Industrial ParkCd.CuauhtemocChihuahuaC.P.31570Mexico School of Chemistry Autonomous University of CoahuilaBlvd.V.Carranza y Jose Cardenas s/nSaltilloCoahuilaC.P.25000Mexico

The control of Spodoptera frugiperda is based on synthetic insecticides,so some alternatives are the use of entomopathogenic fungi(EF)and neem *** objective of the study was to evaluate in vitro effectiveness of native EF and neem extracts on *** *** EF were identified by DNA sequencing of ITS regions from three EF(Fusarium solani,Metarrhizium robertsii,Nigrospora spherica and Penicillium citrinum).They were evaluated in concentrations of 1×10⁸spores/*** addition,a second bioassay was carried out evaluating only ***,*** and *** and the addition of vegetable *** the other hand,extraction of secondary metabolites from neem seed(Azadirachta indica)was carried out by performing,mass(g)and solvent volume(mL ethanol and water)combinations,which were subjected to microwaves and ***,these extracts were evaluated in concentrations of 3%,4%and 5%.A survival analysis was performed for each of the *** respect to the results of the first bioassay,*** obtained a probability of survival of 0.476 on the seventh day,while in the second bioassay,*** obtained 0.488 survival *** suggests that the expected percentage of larvae that stay alive on the sixth day is 48.8%.However,in the evaluation of the neem extract the combination 1:12/70%to 4%caused 84% mortality of *** use of native HE and neem extracts has potential for the control of ***.

关键词： Bioinsecticide biological control Fall armyworm Extracts Seed.

Two new species of Eurytoma(Hymenoptera:Eurytomidae),parasitoids of Bradybatus sp.(Coleoptera:Curculionidae)damaging seeds of Acer truncatum in China

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Zoological Systematics 2023年第3期48卷 264-276页

作者： Huihui Liu Liangming Cao Xiaoyi Wang Xuebing Xin Yingxia Bao Zhongqi Yang Experimental Center of Forestry in North China CAF/National Permanent Scientific Research Base for Warm Temperate Zone Forestry of Jiulong Mountain in BeijingBeijing 102300China Ecology and Nature Conservation Institute Chinese Academy of Forestry&Key Laboratory of Forest Protection of National Forestry and Grassland AdministrationBeijing 100091China Wujiaofeng Nature Reserve of Horqinyouyizhongqi Xing'an LeagueInner Mongolia 137400China

Two new species of Eurytoma,*** Yang,Liu&Cao,*** *** Yang,Liu&Cao,***.,are described and illustrated,as the solitary larval and pupal ectoparasitoid of Bradybatus sp.(Coleoptera:Curculionidae),a serious pest of purpleblow maple,Acer truncatum Bunge(Aceraceae).Photographs of the characters of the new species were *** materials were deposited in Insect Museum,Chinese Academy of Forestry,*** biological characters observed and their biocontrol potential were also discussed.

关键词： Eurytoma seed-eater parasitoids biological control Acer truncatum

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Sclerodermus alternatusi(Hymenoptera:Bethylidae),a new species from China,parasitizing Monochamus alternatus(Coleoptera:Cerambycidae)

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Zoological Systematics 2024年第3期49卷 258-266页

作者： Zhongqi Yang Xiaoyi Wang Zhaoyao Duan Yanlong Zhang Yi’nanZhang Liangming Cao Ke Wei Key Lab of Forest Protection National Forestry and Grassland AdministrationEcology and Nature Conservation InstituteChinese Academy of ForestryBeijing 100091China Research Institute of biological control Beijing Vocational College of AgricultureBeijing 102442China Research Institute of Forest Protection Yunnan Academy of Forestry and GrasslandKunming 650201China

A new species,Sclerodermus alternatusi Yang,***.(Hymenoptera:Bethylidae),is described and *** cerambycid host,Monochamus alternatus Hope,a severe wood borer attacking many pine trees(Pinus spp.),is also a vector of pine wood nematode,Bursaphelencus xylophilus,which cause severe damages to pine forests in *** new species is a gregarious ectoparasitoid of junior larva of *** number of adult wasps reared from a single host larva ranges from 6 to *** ratio of female to male is 20:*** new parasitoid species has a high potential in using as a biocontrol agent for the wood *** of the new species with comparisons to its related species,*** Yang&Yao and a key to known species of Sclerodermus from China is provided.

关键词： Ectoparasitoid larval parasitoid taxonomy biological control

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Life history and life table analysis of the whitefly predator Delphastus catalinae （Coleoptera： Coccinellidae） on collards

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Insect Science 2005年第2期12卷 129-135页

作者： TONG-XIANLIU Vegetable IP Mlaboratory Department of EntomologyTexas Agricultural Experiment StationTexas A&M UniversityWeslacoTexasUSA

The ladybeetle, Delphastus catalinae (Horn), is one of the most commonly usedpredacious natural enemies being commercially reared for controlling whiteflies, including Bemisiatabaci (Gennadius) biotype B (= B. argentifolii Bellows and Perring), on various ornamental andvegetable crops under greenhouse conditions. The development, survivorship, and fecundity of *** feeding on B. tabaci biotype B on collard plants were determined in the laboratory, andthe age-specific life table parameters were analyzed based on the life history data. Developmentaltime was 4.0,1.9,1.1,1.4,5.2, and 5.3 days for eggs, first, second, third, fourth instars, andpupae, respectively, with an average of 18.9 days from oviposition to adult emergence for bothsexes, 19.0 days for females, and 18.8 days for males. Adult longevities averaged 146.6 days forboth sexes, 122.6 days for females, and 170.5 days for males. After an average 4.9 dayspreoviposition period, females laid a mean of 5.6 eggs per day over a 97.0-day period. Netreproductive rate (R_0) and gross reproductive rate (Σm_x) were estimated by life table analysis at276.8 and 325.1, respectively. Generation time (T) and doubling time (DT) were 35.6 and 4.8 daysrespectively, and the intrinsic rate of natural population increase (r_m) was estimated at 0.158, orl= 1.171 for the finite rate of increase. The r_m value of D. catalinae is similar to or higherthan those of the whitefly feeding on most vegetable and ornamental crops, indicating that theladybeetle is capable of regulating populations of B. tabaci biotype B and other whiteflies undergreenhouse conditions.

关键词： coccinellids whiteflies biological control greenhouse life table predation

Isolation and Characterization of Bacillus Amyloliquefaciens L-1 for Biocontrol of Pear Ring Rot

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Horticultural Plant Journal 2017年第5期3卷 183-189页

作者： SUN Pingping CUI Jianchao JIA Xiaohui WANG Wenhui Institute of Pomology Chinese Academy of Agricultural Sciences

Considering the heavy losses caused by pear ring rot(Botryosphaeria berengeriana) disease, the potential biocontrol agent against B. berengeriana was isolated and characterized in this work. Bacteria, isolated from pear orchard rhizosphere soil, were screened for the biological control of pear ring rot caused by B. berengeriana. Among them, strain L-1 showed noticeable inhibitory activities against B. berengeriana and six other commonly occurring postharvest pathogens. Molecular methods indicated strain L-1 was Bacillus amyloliquefaciens. The potential of strain L-1 as an effective biocontrol agent was further estimated. Results showed strain L-1 could successfully colonize in pear wounds, its colonies reached 142.35 folds on 4 days post inoculation, and maintained at a high level during storage. In addition, strain L-1 caused abnormal hyphae growth of B. berengeriana,and its inhibitory percentage against pear ring rot reached 76.55% in vivo on 11 days post inoculation. Strain L-1 significantly induced the peroxidase(POD) and catalase(CAT) activities and delayed the accumulation of malondialdehyde(MDA) in pears. What's more, strain L-1 did not impair the fruit quality. All these results suggest that B. berengeriana L-1 is a promising agent for the biocontrol of pear ring rot.

关键词： pear ring rot Bacillus amyloliquefaciens biological control fruit quality parameters

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