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Technological Feasibility of biodiesel production from Bioaugmented Hydrolysate of Waste Sludge in a Municipal Wastewater Treatment Plant

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Transactions of Tianjin University 2013年第5期19卷 332-337页

作者：王怡赵景婵曲鹏程张百鑫彭党聪夏四清 School of Environmental and Municipal Engineering Xi’an University of Architecture and Technology College of Chemistry & Materials Science Northwest University School of Environmental Science and Engineering Tongji University

The hydrolysate of waste sludge was used as the feedstock of biodiesel production,and its technological feasibility was *** sludge,collected from No.3 Municipal Wastewater Treatment Plant of Xi’an,was hydrolyzed in two parallel reactors *** was added into one reactor for bioaugmentation,and the other reactor without yeast was used as a *** an acid-catalyzed in situ esterification process was carried out to convert the hydrolysate to *** results of hydrolysis showed that the reactor bioaugmented with yeast could promote hydrolysis compared with the control one because of an obvious variance in total suspended solid(TSS),volatile suspended solid(VSS)and soluble chemical oxygen demand(SCOD).Furthermore,gas chromatography(GC)analysis exhibited that the total volatile fatty acid(VFA)was low in the hydrolysate of bioaugmentation reactor;however,its yield of the fatty acid methyl esters(FAMEs)by in situ esterification was obviously higher when compared with the control ***,it may be inferred that the hydrolysate of bioaugmentation was mainly inclined to longer-chain fatty acid rather than to ***,an FAMEs yield of 9.24%(wt%)from dried sludge was attained after the 12-d bioaugmentation hydrolysis and succedent *** value was not only higher than that of the control one but also higher than that reported in previous *** above results illuminated that it was feasible to produce biodiesel from the bioaugmented hydrolysate of waste sludge.

关键词： waste sludge hydrolysis bioaugmentation biodiesel production feasibility

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Reaction-controlled microgel-lipase co-stabilized compartmentalized emulsion for recyclable biodiesel production

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Aggregate 2023年第3期4卷 153-161页

作者： Xin Guan Zhili Wan To Ngai Department of Chemistry The Chinese University of Hong KongHong KongChina Laboratory of Food Proteins and Colloids School of Food Science and EngineeringSouth China University of TechnologyGuangzhouChina

Pickering emulsions have been widely used for biphasic catalysis in the past ***,it remains a great challenge to achieve simple product collection and enzyme ***(N-isopropylacrylamide)(PNIPAM)-based microgels can endow Pickering emulsions with stimuli-responsiveness,while most microgelstabilized emulsions are oil-in-water(O/W)type and not ideal for interfacial ***,altering temperature or pH value for demulsification is time-and energy-consuming and may cause irreversible deactivation of *** this work,inverse water-in-oil(W/O)Pickering emulsions were formed using hexanoic acidswollen microgels as the sole *** lipase was added in the water phase,stable oil-in-water-in-oil(O/W/O)Pickering double emulsions could be formed through one-step emulsification,owing to the synergistic effect of the hydrophobic microgels and hydrophilic lipase at the *** with other biphasic systems,such double emulsion systems represent a desirable platform for highly efficient biodiesel production because of the ultra-high interfacial areas and fast mass transport between two *** importantly,the switchable transition between hydrophobicity/hydrophilicity of microgels is controlled by the catalytic ***,double emulsions demulsify spontaneously when substrates are used up without the need for energy input or loss of enzymatic activity,enabling the facile collection of products and demonstrating the excellent recyclability of the biphasic catalysis system.

关键词： biodiesel production lipase microgel Pickering double emulsion recyclability

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biodiesel production by Enzymatic Catalysis Process Using Two Analytical Ways： Gas Chromatography and Total Glycerol Determination

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Journal of Chemistry and Chemical Engineering 2014年第7期8卷 717-726页

作者： Ignacio Contreras Andrade Jonathan Parra Santiago Jose Ricardo Sodre Joseph Sebastian Pathiyamattom Carlos Alberto Guerrero-Fajardo Doctor Chemical Engineering Universidad Autonoma de Sinaloa Culiacan 80040 Mexico Chemical Engineering (C) Universidad Nacional de Colombia Bogota11001 Colombia Coordinator of the Postgraduate Program in Mechanical Engineering Pontifical Catholic University of Minas Gerais BeloHorizonte 30130 Brazil Center forEnergy Research Universidad Nacional Autonoma de Mexico Temixco 62580 Mexico Chemical Engineering and Mechanical Universidad Nacional de Colombia Bogota 11001 Colombia

Currently, biodiesel is presented as one of the best alternatives for gradually replacing the use of fossil fuels, but it has some factors that make it economically impractical if it does not have a government support. For this reason, research efforts focused on this area have been responsible for optimizing the process of biodiesel production by different catalytic routes to achieve greater efficiency at a lower cost. In this case, the biggest problem has been the high cost generated by an investigation, which in many occasions is the main factor to decide if an investigation could be carried out. Trying to reduce these costs, in the current study, we are using a technique of glycerol quantification by volumetric methods and comparing obtained results with the chromatographic method, which is conventionally used and comparatively much more expensive. biodiesel employee was obtained by an enzymatic catalysis process varying one of three process variables：oil：alcohol molar ratio, temperature and proportion of catalyst. The numerical differences obtained between the two quantification methods generated relative errors lower than 10%, resulting in some occasions lower than 1%. By gas chromatography analysis the best yield was obtained at the same conditions of the volumetric method, a temperature of 45 ℃, an oil：alcohol ratio 1：4 and 8 wt.% of catalyst, but a yield of 95.5% and 97.1%, respectively. Due to the high precision of gas chromatography, this method is used to carry out a surface response analysis obtaining as ideal operating conditions a temperature of 43.5 ℃, 8.9 wt.%. of catalyst and an oil：alcohol ratio 1：4.

关键词： biodiesel production glycerol determination gas chromatography enzymatic catalysis.

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Potential of <i>Dictyosphaerium</i>sp. LC172264 Concomitant Remediation of Cassava Wastewater and Accumulation of Lipids for biodiesel production

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Advances in Bioscience and Biotechnology 2021年第8期12卷 257-274页

作者： Innocent Okonkwo Ogbonna Joseph Ikwebe Otsai Otsima Okpozu Chijioke Nwoye Eze James Chukwuma Ogbonna Department of Microbiology Federal University of Agriculture Makurdi Nigeria Department of Biochemistry Federal University Wukari Nigeria Environmental Science Unit Federal University of Agriculture Makurdi Nigeria Department of Science Laboratory Technology University of Nigeria Nsukka Nigeria Department of Microbiology University of Nigeria Nsukka Nigeria

As a way of making algal feedstock feasible for biofuel production, simultaneous utilization of microalga Dictyosphaerium sp. LC172264 for cassava wastewater remediation and accumulation of lipids for biodiesel production was investigated. The algal biomass, lipid contents and composition were measured from the autotrophic, heterotrophic and mixotrophic cultured algal cells. Physicochemical parameters of the cassava wastewater and bioremediation potentials were measured. biodiesel properties were deduced and compared with the standards. The results showed that mixotrophic culture was the best for both biomass accumulation (1.022 g/L) and lipid contents (24.53%). Irrespective of the culture condition, the predominant fatty acids were similar and included 11-Octadecenoic acid (vaccenic acid (C19H36O2), oleic acid (C18H34O2) and 14-methyl pentadecanoic acid (isopalmitic acid (C17H34O2). The percentage reduction of total dissolved solids was 79.32% and 89.78% for heterotrophy and mixotrophy respectively. Biochemical oxygen demand was 72.95% and 89.35%, chemical oxygen demand was 72.19% and 84.03% whereas cyanide contents reduced from the initial value of 450 mg/L to 93.105 (79.31%) and 85.365 mg/L (81.03%) respectively. Dictyosphaerium sp. showed good growth and lipid production under mixotrophic condition and produced good quality biodiesel under the three cultivation modes. Even though both mixotrophic and heterotrophic conditions had good promise of cassava wastewater remediation by Dictyosphaerium sp., mixotrophy showed superiority.

关键词： biodiesel production Cassava Wastewater Dictyosphaerium sp. Fatty Acid Profile

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