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Effect of leachate recycling and inoculation on the biochemical characteristics of municipal refuse in landfill bioreactors

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Journal of Environmental Sciences 2002年第3期14卷 406-412页

作者： ShenDS HeR DepartmentofEnvironmentandResource ZhejiangUniversityHangzhou310029China.E－mail：shends@*** DepartmentofEnvironmentandResource ZhejiangUniversityHangzhou310029China.E－mail：shends@mail.hz.zj

Activity development of key groups of enzymes involved in municipal refuse decomposition was measured in laboratory landfill bioreactors with and without leachate recycling and inoculation for about 210 days. The results showed that the enzymes (amylase, protease, cellulase, lipase and pectinase) were present in fresh refuse but at low values and positively affected by leachate recycling and refuse inoculation. The total average of cellulase activity in digesters D3 operated with leachate recycling but no inoculation, D4 and D5 operated with leachate recycling and inoculation was much higher than that in digesters D1 and D2 without leachate recycling and inoculation by 88%—127%, 117%—162% and 64%—98%. The total average of protease activity was higher in digester D4 than that in digesters D1, D2, D3 and D5 by 63%, 39%, 24% and 24%, respectively, and the positive effect of leachate recycling and inoculation on protease activity of landfilled refuse mainly was at the first two months. The total average of amylase activity was higher in digesters D3, D4 and D5 than that in digesters D1 and D2 by 83%—132%, 96%—148% and 81%—129%. During the early phase of incubation, the stimulatory effect of inoculation on lipase activity was measured, but refuse moisture was the main factor affecting lipase activity of landfilled refuse. The inoculation, initial and continuous inoculation of microorganisms existing in leachate, was the mainly stimulatory factor affecting pectinase activity of landfilled refuse.

关键词： municipal refuse landfill bioreactor leachate recycle inoculation enzyme activity biochemical characteristics

Comparative studies on the biochemical characteristics of natural actomyosin isolated from PSE and normal pork

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Journal of Zhejiang University Science 2004年第6期5卷 684-688页

作者：丁玉庭桑卫国陈艳 CollegeofBiology＆EnvironmentEngineering ZhejiangUniversityofTechnologyHangzhou310014China FacultyofLifeScience＆Biotechnology NingboUniversityNingbo315211China

biochemical changes of natural actomyosin from fresh pale, soft, exudative (PSE) and normal pork were studied,and the effects of different storage temperatures and different incubation temperature and times on sample superprecipitation,total sulfhydryl (-SH) content, and ATP (adenosine triphosphate) sensitivity were investigated. The results demonstrated that ATPase activity and thermal stability of PSE actomyosin were lower than those of normal pork; and that PSE actomyosin had higher -SH content than that of normal pork at all incubation temperatures and times tested.

关键词： PSE Pork Actomyosin biochemical characteristics

Physiological and biochemical characteristics in Flag Leaves of the C Liangyou Series of Hybrid Rice Combinations at Late Growth Stages

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Rice science 2010年第4期17卷 319-325页

作者： TANG Wen-bang ZHANG Gui-lian XIAO Ying-hui DENG Hua-bing FAN Ke LIU Guo-hua CHEN Li-yun Rice Research Institute Hunan Agricultural University Changsha 410128 China

The contents of chlorophyll, soluble sugars, soluble proteins and thiobarbitudc acid reaction substance （TBARS）, chlorophyll fluorescence parameters, net photosynthetic rate as well as the activities of superoxide dismutase （SOD） and peroxidase （POD） of flag leaves at the late growth stages were studied by using C Liangyou series of hybrid rice combinitions as material and Shanyou 63 as control. The C Liangyou series of hybrid rice combinations used in the experiment included C Liangyou 396, C Liangyou 87, C Liangyou 755 and C Liangyou 34, which all used C815S as male sterile line. The contents of chlorophyll, soluble sugars and soluble proteins in flag leaves of the C Liangyou series combinations at the late growth stages were higher than those of the control, whereas the TBARS content was lower than that of the control. The activities of SOD and POD were significantly higher than those of the control on the 7th day after heading, and then decreased slowly. ~bps, value and qp value of flag leaves decreased at the late growth stages, and these two parameters in flag leaves of the C Liangyou series combinations were higher than those of the control, while the qN value increased at the late growth stages and was lower than that of the control. The net photosynthetic rate of flag leaves at the late growth stage was higher compared with the control. These results suggest that slow senescence and strong photosynthetic capability in flag leaves at the late growth stages are the physiological basis of the C Liangyou series combinations.

关键词： hybrid rice physiological characteristics biochemical characteristics functional leaf

Physiological Responses of Dendrobium officinale under Exposure to Cold Stress with Two Cultivars

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Phyton-International Journal of Experimental Botany 2020年第3期89卷 599-617页

作者： Shuaishuai Zhang Jin Li Yongchang Shen Linda Nartey Korkor Qian Pu Jin Lu Balock Shakeela Dedong Kong Ou Li Guohong Zeng Xiufang Hu Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation College of Life ScienceZhejiang Sci-Tech UniversityHangzhou310018China College of Agriculture and Biology Zhejiang UniversityDeqing Ecoagriculture Biotechnology Co.Ltd.Hangzhou310018China

This study aimed to explore the cold tolerance of two cultivars of Dendrobium officinale(MG1,MG2)grown in different regions of ***-2℃ incubation,cultivar MG1 remained active after 3 d,and continued to grow after returning to room ***,MG2 could only maintain its activity after 2 d treatment at−2℃,and the seedlings died with the low temperature treatment *** of the characteristics of the plants grown in the south(Hangzhou)or north(Zhengzhou)of China indicated that the leaves of MG1 also had reduced stomatal density,the highest thickness,and a compact *** contents of proline and soluble sugars were higher in MG1 than those in *** cultivar MG1 had higher SOD enzyme activity than MG2,while CAT and POD activities in samples from Zhengzhou were higher than those from *** contents of polysaccharides and alkaloids in stems of in MG1 were higher than those in MG2,while the content of flavonoids in the Zhengzhou samples was higher than that in the Hangzhou *** addition,plant heights,stem diameters,and chlorophyll content were higher in ***,MG1 had better cold resistance than ***1 is a cold tolerant cultivar with thick leaves and reduced stomatal density,higher contents of soluble sugars,proline,CAT,POD,polysaccharides,flavonoids and alkaloids,which together make it more adaptable to low ***,the cultivar MG1,with its demonstrated cold tolerance,can accordingly be grown on a large scale in cold regions,thereby expanding the available planting area for this important traditional medicinal plant to meet the increasing commercial demand for it.

关键词： Dendrobium officinale cold stress microstructure characteristics physiological characteristics biochemical characteristics

Clinical Features and Genetic Analysis of Pediatric Patients with Alagille Syndrome Presenting Initially with Liver Function Abnormalities

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Current Medical Science 2018年第2期38卷 304-309页

作者： Yan LIU Hong WANG Chen DONG Jie-xiong FENG Zhi-hua HUANG Department of Pediatrics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Genetic Diagnostic Centre Department of Internal Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Department of Pediatric Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Alagille syndrome （AGS） is a multisystem disorder and caused by mutations in JAG1 or NOTCH2 gene. The diagnosis of AGS is hampered by its highly variable clinical manifestations. We performed a retrospective analysis on 16 children diagnosed as having AGS in recent five years in our hospital. Cholestasis was seen in 15 patients （93.8%）, heart disease in 12 （75%）, characteristic facies in 7 （43.8%）, and butterfly vertebrae in 7 （43.8%）. Ophthalmology examination was not performed on all the patients. Further, serum biochemical parameters were compared between AGS and 16 biliary atresia （BA） patients who were confirmed by surgery. Elevated liver enzymes were seen in all the patients. Serum total cholesterol （TC）（P=0.0007）, alanine aminotransferase （ALT）（P=0.0056）, aspartate aminotransferase （AST）（P=0.0114）, gamma-glutamyl transferase （GGT）（P=0.035） and total bile acid （TBA） levels （P=0.042） were significantly elevated in AGS patients compared to those in BA cases. However, there were no significant differences in serum total bilirubin （TB）, conjugated bilirubin （CB） and albumin （ALB） between the two groups. We identified 14 different JAG1 gene variations and 1 NOTCH2 gene mutation in 16 Chinese AGS patients. Our study suggested clinical features of AGS are highly variable and not all patients meet the classical diagnostic criteria. It was suggested that hypercholesterolaemia and significantly elevated GGT, TBA and ALT may be helpful to diagnose AGS. Genetic testing is integral in the diagnosis of AGS.

关键词： Alagille syndrome cholestasis biochemical characteristics gene infant

Clinical characteristics in Patients with Liver Cirrhosis Induced by HBV Infection and Combined with Mild Alcohol Intake

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国际感染病学（电子版） 2014年第3期3卷 97-102页

作者： Ha-lida Xiaerfuhazi Hai-lin Ma Xiu-jiang Shi Xiao-tang Fan Xi-ernayi Abuduheilili Fang-ping He Department of Hepatology the First Hospital Affiliated to Xinjiang Medical University

Objective To investigate the differences of clinical and biochemical characteristics between patients with liver cirrhosis induced by HBV infection combined with and without mild alcohol *** Data of patients with liver cirrhosis who were hospitalized in the First Hospital Affiliated to Xinjiang Medical University were retrospectively analyzed. Patients were divided into three groups: patients with liver cirrhosis induced by HBV infection and combined with mild alcohol intake, patients with HBV-related cirrhosis, and patients with alcohol-related cirrhosis. biochemical detections including liver function, fasting lipid profiles, lipoprotein, kidney function, glucose, uric acid and regular blood tests were carried out and results were compared among three groups. Data were analyzed through STATA software and co-variant analysis. Results Total of 2 350 patients with liver cirrhosis were included, 732 patients had cirrhosis induced by HBV infection combined with mild alcohol intake, 1 316 patients had HBV-related liver cirrhosis, 302 patients had alcohol-related cirrhosis. The highest mean level of white cell count, mean corpuscular volume, γ-glutamyltranspeptidase and uric acid were observed in HBV infection combined with mild alcohol intake group. Multivariate regression analysis revealed that HBV infection, excessive alcohol intake, male and age were risk factors for hepatocellular carcinoma(HCC) in patients with liver cirrhosis. Conclusions HBV infection combined with mild alcoholic-related liver cirrhosis group showed the highest oxidative stress compared with alcoholic liver cirrhosis group, which suggested that mild alcohol intake may increase the incidence of liver cirrhosis in HBV infected patients and may not increase the incidence of HCC.

关键词： Liver cirrhosis Oxidative stress Hepatitis B virus Mild alcohol biochemical characteristics

Functional characterization of an invertase gene,PstINV1 from Puccinia striiformis ***

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Functional characterization of an invertase gene,PstINV1 fro...