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biochanin a attenuates spinal cord injury in rats during early stages by inhibiting oxidative stress and inflammasome activation

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Neural Regeneration Research 2024年第9期19卷 2050-2056页

作者： Xigong Li Jing Fu Ming Guan Haifei Shi Wenming Pan Xianfeng Lou Department of Orthopedics The First Affiliated Hospital of Zhejiang UniversityHangzhouZhejiang ProvinceChina Department of Stomatology Xixi HospitalHangzhouZhejiang ProvinceChina Department of Orthopedics and Spine Surgerythe Affiliated Hospital of Xuzhou Medical Schoolthe Second People’s Hospital of ChangshuChangshuJiangsu ProvinceChina

Previous studies have shown that biochanin a,a flavonoid compound with estrogenic effects,can serve as a neuroprotective agent in the context of cerebral ischemia/reperfusion injury;howeve r,its effect on spinal cord injury is still unclea r. In this study,a rat model of spinal cord injury was established using the heavy o bject impact method,and the rats were then treated with biochanin a(40 mg/kg) via intrape ritoneal injection for 14 consecutive *** res ults showed that biochanin a effectively alleviated spinal cord neuronal injury and spinal co rd tissue injury,reduced inflammation and oxidative stress in spinal cord neuro ns,and reduced apoptosis and *** addition,biochanin a inhibited the expression of inflammasome-related proteins(ASC,NLRP3,and GSDMD)and the Toll-like receptor 4/nuclear factor-κB pathway,activated the Nrf2/heme oxygenase 1 signaling pathway,and increased the expression of the autophagy markers LC3 Ⅱ,Beclin-1,and *** r,the therapeutic effects of biochanin a on early post-s pinal cord injury were similar to those of *** findings suggest that biochanin a protected neurons in the injured spinal cord through the Toll-like receptor 4/nuclear factor κB and Nrf2/heme oxygenase 1 signaling *** findings suggest that biochanin a can alleviate post-spinal cord injury at an early stage.

关键词： apoptosis autophagy biochanin a heme oxygenase 1 inflammation Nrf2 protein nuclear factor kappa-B oxidative stress spinal cord injury Toll-like receptor 4

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Effects of biochanin a on lactational performance, nitrogen metabolism, and blood metabolites in dairy cows

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ANIMAL NUTRITION 2024年第3期18卷 441-449页

作者： Xiong, Zhanbo Li, Yanjun Zhang, Xiaoyin Zhang, Shiqi Li, Kexin Zheng, Nan Zhao, Shengguo Wang, Jiaqi Chinese Acad Agr Sci Inst Anim Sci State Key Lab Anim Nutr Beijing 100193 Peoples R China

Optimizing nitrogen utilization efficiency and mitigating nitrogen losses in cows plays a pivotal role in fostering economic sustainability within contemporary agricultural systems. biochanin a (BCA), a natural component in red clover, has the potential to improve nitrogen metabolism in dairy cows. The primary objective of this study was to probe the impact of biochanin a supplementation on lactational performance, nitrogen metabolism, and blood metabolites in dairy cows. A complete randomized block design experiment was conducted over 28 d, involving 36 multiparous Holstein cows (comparable milk yield = 37.1 +/- 2.90 kg, BW = 642 +/- 70.0 kg, days in milk = 92 +/- 8.0 d, and parity = 2.4 +/- 0.50), which were allocated to three treatment groups: the Control group (with 0 g/d BCA), the Low group (with 10 g/d per cow BCA), and the High group (with 40 g/d per cow BCA). biochanin a supplementation improved the lactational performance of cows by increasing milk yield by 6.3% (P = 0.007) and feed efficiency by 12.7% (P = 0.009). Total intestinal apparent digestibility was unaffected by BCA supplementation (P > 0.05), but microbial nitrogen was increased by 30.0% (P = 0.002) for promoting nitrogen utilization efficiency by 20.7% (P = 0.004). Milk competent yields (protein, lactose, and non-fat milk solid) were increased with increasing BCA supplementation (P 0.05). BCA did not affect body health of dairy cows. Additionally, none of the plasma endocrine hormones were affected (P > 0.05). A total of 95 significantly different metabolites were screened from the plasma metabolites of cows in the BCA-added and non-added groups. After performing an enrichment analysis of the metabolic pathways associated with the different metabolites, six specific pathways were iden

关键词： biochanin a Lactational performance Nutritional metabolism Blood metabolite RED-CLOVER SILAGE FED DIETS ALFALFA SILAGE CRUDE PROTEIN EFFICIENCY RUMINANT GRASS RUMEN MATURITY SHEEP

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biochanin a,as the Lrg1/TGF-β/Smad2 pathway blockade,attenuates blood-brain barrier damage after cerebral ischemiareperfusion by modulating leukocyte migration patterns

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BIOCELL 2023年第8期47卷 1869-1883页

作者： LONGSHENG FU JINFANG HU FENG SHAO YAOQI WU WEI BAI MINGJIN JIANG HAO CHEN LIHUA CHEN YANNI LV Department of Pharmacy The First Affiliated Hospital of Nanchang UniversityNanchang330006China Key Laboratory of Modern Preparation of TCM Ministry of EducationJiangxi University of Traditional Chinese MedicineNanchang330006China Translational Medicine Institute of Jiangxi The First Affiliated Hospital of Nanchang UniversityNanchang330006China Neurology Department The First Affiliated Hospital of Nanchang UniversityNanchang330006China

Background:biochanin a is an excellent dietary isoflavone that has the concomitant function of both medicine and *** attenuation function of biochanin a on blood-brain barrier(BBB)damage induced by cerebral ischemia-reperfusion remains ***:C57BL/6 mice were subjected to 1 h middle cerebral artery occlusion(MCAO)followed by 24 h *** infarct volume of the brain was stained by TTC,while leakage of the brain was quantitatively stained by Evans blue,and the neurologic deficit score was ***-induced morphologic changes were observed via immunofluorescence staining,and rolling and adhering leukocytes in venules were observed via two-photon imaging,while the inner fluorescein isothiocyanate-albumin of venules were compared with those of surrounding interstitial area through venular albumin ***:The attenuation effect of biochanin a on tight junction injury was compared in ischemia-reperfusion mice or conventional knockdown of leucine-richα2-glycoprotein 1(Lrg1)*** A could ameliorate BBB injury in mice with cerebral ischemiareperfusion in a dose-dependent manner by strengthening the immunostaining volume of occludin,claudin-5,and zonula *** amoeba morphologic changes of microglial combined with the elevated expression of Lrg1 could be relieved under the treatment of biochanin *** A played a countervailing role on the rolling leukocytes in the vessel,while the leakage of blood vessels was *** A diminished its functions to further improved attenuation for tight junction injury on conventional Lrg1-knockout mice,as well as the inhibition effects on TGF-β1,and the phosphorylation of suppressor of mothers against decapentaplegic 2(Smad2)/Smad2 via western blot ***:biochanin a could alleviate tight junction injury induced by cerebral ischemiareperfusion and blocked the Lrg1/TGF-β/Smad2 pathway to modulate leukocyte migration patterns.

关键词： biochanin a Blockade Leukocyte migration Blood-brain barrier damage Ischemia-reperfusion Lrg1

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biochanin a and CPe-Ⅲ Peptide Improved Hepatic Infl ammation by Regulating the Hepatic Lipid Metabolic Pathways in Diet-Induced Obese Mice

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Transactions of Tianjin University 2018年第3期24卷 234-243页

作者： Zhaohui Xue Ru Wang Haichao Wen Wancong Yu Xiaohong Kou School of Chemical Engineering and Technology Tianjin University Medical Plant Laboratory Tianjin Research Center of Agricultural Biotechnology

biochanin a(BCA) and CPe-Ⅲ peptide, which both exist in chickpea(Cicer arietinum L.), possess significant antihyperlipidemic properties. However, the actualmechanisms ofthose compounds in inhibiting the dysregulation oflipid metabolism and complicated inflammation have not been wellcharacterized. This study investigated the effects ofBCA, CPe-Ⅲ peptide, and combined BCA and CPe-Ⅲ peptide(BC) on the expression ofgenes involved in hepatic lipid and inflammation metabolism. Results demonstrated that BCA, CPe-Ⅲ peptide, and BC significantly attenuated hepatitis and hyperlipidemia by downregulating those genes involved in pro-inflammatory cytokines(TNF-α), hepatic fatty acid(FA) synthesis(ACC1 and FAS), cholesterolmetabolism(SREBP2, HMGCR, and PCSK9), and upregulating key regulators involved in FA oxidation(PPARα and FABP1), lipolysis(ATGL), LDLR, reverse cholesteroltransport(ABCA1, SR-B1, and LXRα), and cholesterolcatabolism(CYP7 A1). Moreover, they also altered the expression oflipid metabolism-related proteins, including SREBP2, PCSK9, LDLR, ABCA1, and CYP7 A1. Finally, these results revealed that the combination treatment ofBCA and CPe-Ⅲ peptide resulted in greater antihyperlipidemic activity compared with individualcompounds.

关键词： biochanin a CPe-Ⅲ peptide Lipid metabolism Infl ammation Gene expression Protein expression

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Preparation of National CRMs of Active Compounds from Natural Products

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China Standardization 2010年第3期55卷 19-25页

作者： Duan Wenjuan Geng Yanling Wang Daijie Zhao Xianen Liu Jianhua Wang Xiao Shandong Analysis and Test Center

According to the standard of GB/T1500.3-2008 and the guidelines of ISO/REMCO, the preparation of National Certified Reference Materials （CRMs） of active compounds was composed of the following two aspects： preparation and certification. This paper presents an example of the preparation of biochanin a to illustrate the preparation of National CRMs of active compounds from natural products. Firstly, the target compound was extracted and purified from crude materials. Then, its chemical structure was identified by UV spectrum, IR spectrum, ESI-MS, and NMR. The purity of the compound was determined by HPLC with the area normalization method. The certified value assessed by comparing the purity values from six independent qualified laboratories and the value of uncertainty were given. Thermal analysis, residual solvent, homogeneity testing and stability testing was tested in the process.

关键词： CRMs biochanin a natural products preparation certification

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