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Investigation of biobutanol Efficiency of Chlorella sp. Cultivated in Municipal Wastewater

Journal of Geoscience and Environment Protection 2018年第10期6卷 40-50页

作者： Melih Onay Computational & Experimental Biochemistry Lab. Department of Environmental Engineering Van Yuzuncu Yil University Van Turkey

Many strains of microalgae can grow in wastewaters through their ability to utilize inorganic nitrogen and phosphorus in wastewater. The content of municipal wastewater changes from a location to others. Biofuel production from municipal wastewater has gained huge importance due to progresses in cultivation of microalgae in wastewaters. biobutanol is produced by the acetone-butanol-ethanol (ABE) fermentation. In this study, we examined the biobutanol production efficiency of Chlorella sp. DEE006 which is cultivated in the municipal wastewater in flat-photobioreactor. Growth of microalgae was monitored at 680 nm using spectrophotometer and the biomass was also pre-treated with acidic hydrolysis (1 M H2SO4). Total carbohydrate and protein contents were measured. Fermented microalgae samples were taken for calculation of biobutanol concentration. We obtained both high biobutanol content (6.23 ± 0.19 g·L−1) and high bioethanol yield 0.16 ± 0.005 g (g sugar)−1. 50% wastewater had the highest biomass concentration (1930 ± 11 mg/L) among the wastewaters with five various concentrations. It had the highest biomass productivity with 0.28 ± 0.001 g L−1d−1. Also, it obtained the highest carbohydrate and protein concentration with 0.80 ± 0.02 gL−1 and 0.95 ± 0.01 gL−1, respectively. According to our results, Chlorella sp. DEE006 can be used for large scale biobutanol production in the future.

关键词： Microalgae Chlorella biobutanol Biofuel Municipal Wastewater

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Greener approach to the comprehensive utilization of algal biomass and oil using novel Clostridial fusants and bio-based solvents

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Engineering Microbiology 2023年第2期3卷 16-23页

作者： Asma Fiayaz Yaser Dahman Department of Chemical Engineering Toronto Metropolitan University350 Victoria StTorontoOntario M5B 2K3Canada

A greener method has been tested to utilize algal biomass as a feedstock to produce bio-oil in addition to acetone,butanol,and ethanol(ABE)*** hydrolysis treatments were used prior to fermentation including combination of thermal,chemical,and enzymatic,which resulted in maximum sugar release of 27.78 g/***-based terpenes was used instead of common toxic chemicals together with Clostridial fustants to produce bio-alcoholic *** fusion technique were used to produce the novel Clostridia fusants(***+*** and ***+***).Fused strains were then subjected to UV radiation for strain *** fusansts showed clear improvement in thermal stability and resistance to biobutanol *** experiments showed maximum biobutanol final production of 7.98 g/L using CbCt versus 7.39 g/L using *** extraction from virgin algae was tested using a green,bio-based approach using ter-penes with ultrasonication and green Bligh and Dyer method,*** preliminary study on algal biomass,the combinations of ultrasonication followed by the green Bligh and Dyer have resulted in oil yield of 46.27%(d-limonene)and 39.85%(p-cymene).Oil extraction from an algae sample following fermentation using the combined extraction method resulted in significantly higher oil yield of 65.04%.

关键词： Algal biomass biobutanol Biofuels Simultaneous hydrolysis and fermentation Renewable sources Green products Separate hydrolysis and fermentation Green oil extraction

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Improved Acetone-Butanol-Ethanol (ABE) Solution Analysis Using HPLC: Chromatograph Spectrum Deconvolution Using Asymmetric Gaussian Fit

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American Journal of Analytical Chemistry 2014年第16期5卷 1078-1089页

作者： Niloofar Abdehagh Mehran Bagheri Fatma Handan Tezel Jules Thibault Chemical and Biological Engineering Department University of Ottawa Ottawa Canada Department of Physics University of Ottawa Ottawa Canada

Currently, the analysis of acetone-butanol-ethanol (ABE) broths is performed using both High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC) for each sample since GC cannot be used in quantifying sugars and HPLC methods are not yet efficient enough to detect all components separately. In this study, a novel method was developed to quantify all main components present in ABE model solutions (acetone, butanol, ethanol, butyric acid, acetic acid, glucose and xylose) using only HPLC. Although the HPLC operating conditions were optimized to obtain the best possible resolution in HPLC chromatograms, it was observed that the peaks for butyric acid, acetone and ethanol overlapped. The same trend was observed for glucose and xylose. Using the asymmetric Gaussian fit, a program was written in MATLAB to detect the overlapped peaks, deconvolute them and calculate the area of each separated peak. The concentrations of each component were then calculated using the areas and the calibration curves for each component. Experimental results show that this method works well for the ABE model solutions and can be used to quantify all components in the solution when there are some overlapped peaks in the HPLC chromatograms.

关键词： High Performance Liquid Chromatography Overlapped Peaks Deconvolution ABE Broths Analysis biobutanol

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