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Solvent-solvent fractionations of Combretum erythrophyllum(Burch.) leave extract: Studies of their antibacterial, antifungal, antioxidant and cytotoxicity potentials

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Asian Pacific Journal of Tropical Medicine 2017年第7期10卷 733-741页

作者： Fanyana M.Mtunzi Ikechukwu P.Ejidike Imelda Ledwaba Aroke Ahmed Vusumzi E.Pakade Michael J.Klink Sekomeng J.Modise Department of Chemistry Vaal University of Technology Phytomedicine Programme Department of Paraclinical SciencesFaculty of Veterinary SciencesUniversity of Pretoria ICBT Vaal University of Technology

Objective: To evaluate the biological activities of Combretum erythrophyllum(C. erythrophyllum) leaf extracts against infectious diseases' pathogenesis and their cytotoxicity potentials. Methods: Powdered leaf material(300 g) of C. erythrophyllum was extracted(1:10 w/v) using acetone to obtain the crude extract. Liquid-liquid fractionation was performed on the crude acetone extract(30 g) using solvents of different polarity. The bioautographic method was used to detect the inhibition of bacterial and fungal growth by active compounds present in the crude and fractions. The extracts were then tested on bacterial strains: Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa; fungal strains: Candida albicans(C. albicans), Cryptococcus neoformans, and Aspergillus fumigatus, by microtitre dilution method for MIC determination. Results: The extracts MIC values ranged between 0.08–2.50 mg/m L against the tested pathogens. Water fraction had the highest activity against bacteria strains, while the fungal assay revealed crude acetone extract and ethyl acetate fraction to be active against C. albicans(1.25 mg/m L), dichloromethane extract against C. albicans and Aspergillus fumigatus(0.16 mg/m L). Extract fractions showed a good antioxidant activity via DPPH, ABTS and hydroxyl radical scavenging assays, in the order: ethyl acetate > water > acetone > dichloromethane > hexane. The toxicity level of crude extract and fractions evaluated in Vero monkey kidney cells ranged from 34–223 μg/m L, while doxorubicin(IC_(50) = 7.19 μg/m L) served as the positive control. Conclusions: It can be concluded that the extracts of C. erythrophyllum are safe for medicinal use in folk medicine for treating infectious and stress related diseases.

关键词： Combretum erythrophyllum bioautography Antibacterial Antifungal Antiradical Cytotoxicity

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Antifungal Activity of Poncirus trifoliata Roots against Colletotrichum Species

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Agricultural Sciences 2023年第3期14卷 346-355页

作者： Kumudini M. Meepagala Tamang Prabin USDA-ARS Natural Products Utilization Research Unit University MS USA

Fruits and vegetables are an essential part of a healthy diet, providing humans with vitamins, phytonutrients, and minerals. They are significantly vulnerable, however, to post-harvest diseases caused by numerous fungal and bacterial pathogens. These pathogens can cause significant quantitative and qualitative losses from harvest to consumption during the handling and storage processes. Chemical fungicides are commonly used but are likely to leave residues on the produce, rendering short shelf-life produce, such as berries, unsuitable for human consumption. Identifying eco-friendly methods to control post-harvest disease is, therefore, of utmost importance. The presence of antifungal constituents in the roots of Poncirus trifoliata extracts was detected by thin layer chromatography-based bioautography. The active constituents were isolated and identified by bioautography assay-guided fractionation using flash chromatography followed by spectroscopic techniques. In this study, xanthoxyletin, demethylsuberosin, dentatin, nordentatin, ponfolin, and clausarin were isolated from the root extracts. The antifungal activity of these compounds was moderate to weak compared to the commercial fungicide captan. This study reports the isolation and identification of natural compounds from Poncirus trifoliata that exhibited antifungal activity against Colletotrichum fragariae and Botrytis cinerea, two major post-harvest pathogens.

关键词： Poncirus trifoliata bioautography Colletotrichum fragariae Fungicide

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Bioassay Directed Identification of Royal Jelly’s Active Compounds against the Growth of Bacteria Capable of Infecting Cutaneous Wounds

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Advances in Microbiology 2013年第2期3卷 138-144页

作者： Mariana C. Garcia Mónica S. Finola Juan M. Marioli Departamento de Química Universidad Nacional de Río Cuarto Río Cuarto Argentina

Antibiotic-resistant bacteria continue to be of major health concern world-wide. Thus, it is of great interest to study the biological properties and determine active compounds in natural products likely to be used as new health remedies. Therefore, the main objective of this work is to test the antibacterial activity of royal jelly samples, defatted royal jelly samples and their ethyl ether extracts against bacteria capable of infecting cutaneous wounds in humans and animals, and to recognize major bioactive compounds by using bioassay directed identification. The microorganisms used in the study were Staphylococcus aureus (including Methicillin-resistant and sensitive strains), Staphylococcus epidermidis, Micrococcus luteus, Streptococcus uberis, Enterococcus faecalis, Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae. The activity of royal jelly samples to inhibit bacterial growth was assessed by using well-difussion tests. Direct bioautography was used to identify bioactivity, and uv-visible spectroscopy and gas chromatography-mass spectrometry were used to identify bioactive compounds. Overall, royal jelly samples showed higher growth inhibition activity against Gram positive bacteria as compared to Gram negative bacteria. The growth of bacterial strains belonging to the Enterococcus and Streptococcus genders was less affected by the presence of royal jelly than bacterial strains of the Staphylococcus gender did. Compounds with antibacterial activity were found in the ethyl ether extract of royal jelly samples. 10-hydroxy-2-decenoic acid was the major component identified in the purified fraction obtained by bioassay guided fractionation of the ethyl ether extract. In conclusion, bioactivity of royal jelly samples is mainly due to their 10-hydroxy-2-decenoic acid content.

关键词： Royal Jelly Antibacterial Activity 10-Hydroxy-2-decenoic Acid bioautography

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