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bioassay-guided in vitro study of the antileishmanial and cytotoxic properties of Bixa orellana seed extract

Journal of Coastal Life Medicine 2014年第6期 484-489页

作者： Marley García Ramón Scull Osmany Cuesta Gaëlle Boulet Louis Maes Paul Cos Lianet Monzote Department of Parasitology Institute of Tropical Medicine“Pedro Kourí”HavanaCuba Department of Chemistry Institute of Pharmacy and FoodHavana UniversityHavanaCuba Laboratory of Microbiology Parasitology and Hygiene(LMPH)Faculty of PharmaceuticalBiomedical and Veterinary SciencesAntwerp UniversityAntwerpBelgium

Objective:To investigate the leishmanicidal effect of the Bixa orellana crude seed extract and its fractions against Leishmania ***:Four main fractions(BO-A,BO-B,BO-C and BO-D)were obtained by exhaustion with solvent with increased polarity from the Bixa orellana crude seed extract and 28 *** antileishmanial activity was evaluated in intracellular amastigotes and the cytotoxicity was assessed in murine intraperitoneal ***:The BO-A and BO-B fractions showed a good antileishmanial activity with IC50 values of(12.9±4.1)and(12.4±0.3)μg/mL,*** sub-fractions BO-B1(IC_(50)=(11.8±3.8)μg/mL)and BO-B3[IC_(50)=(13.6±4.7)μg/mL]also proved to have a good leishmanicidal *** general,the sub-fractions showed a lower toxicity than the crude extract.A selectivity index of 9 indicated a moderate selectivity of the BO-A,BO-B and BO-C fractions and BO-B1 ***:Potential of this plant against cutaneous leishmaniasis should be further investigated.

关键词： Bixa orellana Traditional medicine bioassay-guided fractionation Leishmania amazonensis

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The Cytotoxic Constituents from Marine-derived Streptomyces 3320^(#)

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Journal of Ocean University of China 2006年第1期5卷 75-81页

作者： REN Hong GU Qianqun CUI Chengbin ZHU Weiming Key Laboratory of Marine Drug Chinese Ministry of Education Institute of Marine Drug and Food Ocean University of China Qingdao 266003 P. R. China Science and Technology College of Chemistry and Biology Yantai University Yantai 264005 P.R. China Science and Technology College of Chemistry and Biology Yantai University Yantai 264005 P. R. China Beijing Institute of Pharmacology and Toxicology AMMS Beijing 100850 P.R. China

The present work studies the chemical constituents from marine-derived streptomyces 3320^# and their antitumor activities. The n-BuOH extract of the ferment broth of 3320^# was chromatographed on silica gel, Sephadex LH-20, ODS columns and HPLC to separate the compounds with antitoumor activities. Their structures were identified using IR, UV, NMR, MS spectroscopic techniques and compared with published data. The antitumor activities of the isolates were assayed using SRB method and flow cytometry assay, accompanied with the morphological observation of the cells under light micro- scope against mammalian tsFT210 cells. Ten compounds, cyclo-（Ala-Leu） 1, cyclo-（Ala-Ile） 2, cyclo-（Ala-Val） 3, cyclo- （Phe-Pro） 4, cyclo-（Phe-Gly）5, cyclo-（Leu-Pro）6, 1-methyl-1, 2, 3, 4-tetrahydro-β-carboline-3-carboxylic acid 7, N-（4- hydroxyphenethyl） acetamide 8, 4-methyoxy-l-（2-hydroxy） ethylbenzene 9 and uridine 10, were isolated from the ferment broth of streptomyces 3320^# . Among them, compounds 6, 7, 8 and 10 showed potent cytotoxicity against the tsFT210 cell with the IC50 values of 3.6, 7.2, 5.2 and 1.6 mmol L-1, respectively. Compounds 8, 10 also exhibited apoptosis inducing activity under 2.0 mmol L-1. Compounds 6, 7, 8 and 10 are the principle bioactive constituents responsible for the antitumor activities of marine streptomyces 3320^#. Compound 7 was isolated from this species for the first time.

关键词： marine-derived streptomyces secondary metabolites structural identify bioassay-guided fractionation antitumor activity

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猫爪藤细胞毒活性成分跟踪筛选和分离以及抗氧化活性研究（英文）

猫爪藤细胞毒活性成分跟踪筛选和分离以及抗氧化活性研究（英文）

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第二届国际抑郁共病暨第十二届中国中西医结合基础理论学术研讨会

作者：陈良华谌迪郑志忠刘韶松童庆宣明艳林林河通福建省亚热带植物研究所厦门华侨亚热带植物引种园福建农林大学食品科学学院

Objective:Macfadyena unguis-cati L.(MU) is widely used in folk medicine as anti-inflammatory,anti-malarial and *** study was to evaluate the anticancer and antioxidant potential of the aerial parts of MU and then to identify the main compounds responsible for these ***:Detailed phytochemical investigation on MU was carried out by column *** cytotoxicity of extracts or isolated compounds was assessed by MTT *** antioxidant activities of the samples were estimated by DPPH and ***:Results showed that the CHC13 fraction exhibited the greatest cytotoxic activity on human hepatoma cells SMMC7721 and Bel7402 by MTT *** EtoAc and n-BuOH fractions showed strong antioxidant activities on DPPH radical scavenging and ferric reducing power,which were almost comparable to capacities of the positive controls ***,bioassay-guided fractionation of CHC13 fraction led to the isolation of five known compounds,including decaffeoylacteoside(ML-1),lyoniresinol-3 a-O-P-D-glucopyranside(ML-2),cirsimarin(ML-3),cirsimaritin(ML-4),Ursolic Acid(ML-5).Compounds ML-1,ML-2,and ML-5 were isolated firstly from the title *** compounds were assayed for their cytotoxic activities against three human hepatoma cells SMMC7721,HepG2 and ***:Ursolic Acid was found to be the most potent cytotoxic constituents in *** data provide new insight into the chemical constituents and anticancer activity of ***-cati.

关键词： Macfadyena unguis-cati active constituents anti-cancer anti-oxidation bioassay-guided fractionation ursolic Acid

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